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| 1 | Bioengineering bacterial encapsulin nanocompartments as targeted drug delivery system显示文摘The development of Drug Delivery Systems(DDS)has led to increasingly efficient therapies for the treatment and detection of various diseases.DDS use a range of nanoscale delivery platforms produced from polymeric of inorganic materials,such as micelles,and metal and polymeric nanoparticles,but their variant chemical composition make alterations to their size,shape,or structures inherently complex.Genetically encoded protein nanocages are highly promising DDS candidates because of their modular composition,ease of recombinant production in a range of hosts,control over assembly and loading of cargo molecules and biodegradability.One example of naturally occurring nanocompartments are encapsulins,recently discovered bacterial organelles that have been shown to be reprogrammable as nanobioreactors and vaccine candidates.Here we report the design and application of a targeted DDS platform based on the Thermotoga maritima encapsulin reprogrammed to display an antibody mimic protein called Designed Ankyrin repeat protein(DARPin)on the outer surface and to encapsulate a cytotoxic payload.The DARPin9.29 chosen in this study specifically binds to human epidermal growth factor receptor 2(HER2)on breast cancer cells,as demonstrated in an in vitro cell culture model.The encapsulin-based DDS is assembled in one step in vivo by co-expressing the encapsulin-DARPin9.29 fusion protein with an engineered flavin-binding protein mini-singlet oxygen generator(MiniSOG),from a single plasmid in Escherichia coli.Purified encapsulin-DARPin_miniSOG nanocompartments bind specifically to HER2 positive breast cancer cells and trigger apoptosis,indicating that the system is functional and specific.The DDS is modular and has the potential to form the basis of a multi-receptor targeted system by utilising the DARPin screening libraries,allowing use of new DARPins of known specificities,and through the proven flexibility of the encapsulin cargo loading mechanism,allowing selection of cargo proteins of choice. | Alexander Van de Steen Rana Khalife Noelle Colant Hasan Mustafa Khan Matas Deveikis Saverio Charalambous Clare M.Robinson Rupali Dabas Sofia Esteban Serna Diana A.Catana Konstantin Pildish Vladimir Kalinovskiy Kenth Gustafsson Stefanie Frank | 2021 | Synthetic and Systems Biotechnology2021，6，3： | 2 |
| 2 | Concurrent clarithromycin and cyclosporine A treatment显示文摘 | Sádaba B López de Ocáriz A Azanza J R | 1998 | J Antimicrob Chemother1998，42，3： | 1 |
| 3 | Simple method of purification and sequencing of a bacteriocin produced by Pediococcus ac/rf/7ac^yUL5显示文摘 | Daba H Lacroix C Huang J | 1994 | JAppl Bacteriol1994，77，6： | 1 |
| 4 | A comparative study of the pharmacokinetics of ibuprofen arginate versus dexibuprofen in healthy volunteers显示文摘 | Sádaba B Campanero MA Munoz-Juarez MJ | 2006 | Eur J Clin Pharmacol2006，62，10： | 1 |
| 5 | ABC multidrug transporter Cdr1p of Candida albicans has divergent nucleotide-binding domains which display functional asymmetry显示文摘 | Jha S Dabas N Karnani N | 2004 | FEMS Yeast Res2004，5，1： | 1 |
| 6 | Ibuprofen-arginine pharmacology and role in the treatment of acute pain显示文摘 | Garcia-Quetglas E Sádaba B Azanza JR | 2006 | Dolor2006，21，1： | 1 |
| 7 | ABC multidrug transporter Cdr1p of Candida albicans has divergent nucleotide-binding domains which display functional asymmetry显示文摘 | JHA S DABAS N KARNANI N | 2004 | FEMS Yeast Res2004，5，1： | 1 |
| 8 | Value of helical computed tomography in the management of upper esophageal foreign bodies显示文摘 | Marco De Lucas E Sádaba P Lastra García-Barón P | | 0，，04： | 1 |
| 9 | Chemical modification of wheat 13-amylase by trinitrobenzenesulfonic acid, methoxypolyethylene glycol, and glutaraldehyde to improve its thermal stability and activity 显示文摘 | DABA T KOJIMA K INOUYE K | 2013 | Enzyme and Microbial Technology2013，53，67： | 1 |
| 10 | Screening of potential lacto- bacilli protein-bound exopolysaccharides显示文摘 | MOSTAFA H DABA A el MEZAWY A | 2006 | Deutsche Lebensmittel- Rundschau2006，102，： | 1 |
| 11 | Screening of potential hctobacilli protein-bound exopolysaccharides显示文摘 | MOSTAFA H DABA A EL-MEZAWY A | | 0，，： | 1 |
| 12 | Platelet rich fibrin: a new covering material for oral mucosal defects 显示文摘 | Mohanty S Pathak H Dabas J | 2014 | J Oral Biol Craniofac Res2014，4，2： | 1 |
| 13 | SimpLe method of purification and sequencing of a baeteriocin produced by Pediococcus acidilactici UL5显示文摘 | DABA H LACROIX C HUANG J | 1994 | J Appl Bacteriol1994，77，： | 1 |
| 14 | Design of highly birefringent chalcogenide glass PCF:A simplest design 显示文摘 | DABAS B SINHA R K | 2011 | Optics Communications2011，284，： | 1 |
| 15 | Simulation in the time domain for heterodyne coherent laser radar显示文摘 | Salamitou P Dabas A Flamant P H | 1995 | Appl Opt1995，34，3： | 1 |
| 16 | Repression of nitrogenase by ethanol in nitrogen-deprived cultures of Rhodovu]um sulfdophilum 显示文摘 | Maeda I Daba M Hirose H | 1999 | FEMS Microbiol Lett1999，171，： | 1 |
| 17 | Optimized prodrug approach:A means for achieving enhanced anti-inflammatory potential in experimentally induced colitis显示文摘 | Philip AK Dabas S Pathak K | | 0，，03： | 1 |
| 18 | Early differential diagnosis of multiple sclerosis using a new oligoclonal band test显示文摘 | Villar LM Masjuan J Sádaba MC | 2005 | Arch Neurol2005，62，： | 1 |
| 19 | Effects of L-carnitine and ginkgo biloba extract ( EG b 761 ) in experimental bleomycin-induced lung fibrosis 显示文摘 | Daba MH Abdel-Aziz AA Moustafa AM | 2002 | Pharmacol Res2002，45，6： | 1 |
| 20 | Kinetic and thermodynamic analysis of the inhibitory effects of maltose, glucose, and related carbohydrates on wheat β-amylase显示文摘 | DABA T KOJIMA K INOUYE K | 2013 | Enzyme and Microbial Technology2013，52，： | 1 |
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