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| 1 | Using the polymerase chain reaction coupled with denaturing gradient gel electrophoresis to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis显示文摘AIM: To investigate the use of PCR and DGGE to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted severe AP.METHODS: Patients with biochemical and clinical evidence of acute pancreatitis and an APACHE Ⅱ score ≥8 were enrolled. PCR and DGGE were employed to detect bacterial translocation in blood samples collected on d1,3, and 8 after the admission. Standard microbial blood cultures were taken when there was clinical evidence of sepsis or when felt to be clinically indicated by the supervising team.RESULTS: Six patients were included. Of all the patients investigated, only one developed septic complications;the others had uneventful illness. Bacteria were detected using PCR in 4 of the 17 collected blood samples. The patient with sepsis was PCR-positive in two samples (taken on d 1 and 3), despite three negative blood cultures. Using DGGE and specific primers, the bacteria in all blood specimens which tested positive for the presence of bacterial DNA were identified as E coli.CONCLUSION: Our study confirmed thatunlike traditional microbiological techniques, PCR can detect the presence of bacteria in the blood of patients with severe AP. Therefore, this latter method in conjunction with DGGE is potentially an extremely useful tool in predicting septic morbidity and evaluating patients with the disease. Further research using increased numbers of patients, in particular those patients with necrosis and sepsis, is required to assess the reliability of PCR and DGGE in the rapid diagnosis of infection in AP. | Callum B Pearce Vitaly Zinkevich Iwona Beech Viera Funjika Ana Garcia Ruiz Afraa Aladawi Hamish D Duncan | 2005 | World Journal of Gastroenterology2005，11，45： | 11 |
| 2 | Genetic transformation of wheat mediated by Agrobacterium tumefaciens显示文摘 | Cheng M Fry J E Pang S Zhou H Hironaka C M Duncan D R Conner T W Wan Y | 1997 | Plant Physiol1997，，： | 2 |
| 3 | 2008 estimate of worldwide rotavirus-associated mortality in children younger than 5 years before the introduction of universal rotavirus vaccination programmes: a systematic review and meta-analysis显示文摘 | Jacqueline E Tate Anthony H Burton Cynthia Boschi-Pinto A Duncan Steele Jazmin Duque Umesh D Parashar | 2012 | The Lancet Infectious Diseases2012，，2： | 2 |
| 4 | Comparison of methods to detect biosurfactant production by diverse microorganisms 显示文摘 | Youssef N H Duncan K E Nagle D P | 2004 | Journal of Microbiological Methods2004，56，3： | 1 |
| 5 | Proline accumulaion and its implication incold tolerance of regenerable maize callus显示文摘 | DUNCAN D R Widho W | 1987 | Plant Physiol1987，83，： | 1 |
| 6 | The solar X-ray imaget for GOES显示文摘 | Lemen J R Duncan D Edwards C | 2004 | SPIE2004，5171，： | 1 |
| 7 | Lethal and sub-lethal effects of the salmon delousing agent dichlorvos on the larvae of the lobster (Homarus gammarus L) and herring (Clupea harengus L)显示文摘 | JOHN G M DEREK S DUNCAN D S | 1991 | Aquaculture1991，98，： | 1 |
| 8 | Heterologous protein secre- tion from yeast显示文摘 | Smith R A Duncan M J Moir D T | 1985 | Science1985，20，： | 1 |
| 9 | Delayed sternal closure after cardiac operations in a pediatric population 显示文摘 | Tabbutt S Duncan BW McLaughlin D | 1997 | Thorae Cardio- vase Surg1997，113，5： | 1 |
| 10 | Osmotic induced stimulation of the reduction of the viability dye 2,3,5 triphenyltetrazolium chlo ride by maize roots and callus cultures 显示文摘 | Duncan D R Widholm J M | 2004 | Journal of Plant Physiology2004，161，： | 1 |
| 11 | A novel approach for lignin modification and degradation 显示文摘 | TIAN Min WEN Jiali DUNCAN M D | 2010 | Electrochemistry Communications2010，12，： | 1 |
| 12 | Experimental study of inequality and unpredictability in an artificial cuitural market显示文摘 | MATTHEW J S PETER S D DUNCAN J W | 2006 | Science2006，311，5762： | 1 |
| 13 | A randomized,controlled pilot study of a home-based exercise program for individuals with mild and moderate stroke显示文摘 | Duncan PW Richards L Wallace D | 1998 | Stroke1998，29，10： | 1 |
| 14 | Quality of life,mucositis,and xerostomia from radiotherapy for head and neck cancers:a report from the NCIC CTG HN2 randomized trial of an antimicrobial lozenge to prevent mucositis显示文摘 | Duncan GG Epstein JB Tu D | 2005 | Head Neck2005，27，5： | 1 |
| 15 | Web space reconstruction with a M-V flap显示文摘 | LEWIS R C NORDYKE M D DUNCAN K H | 1988 | J Hand Surg Am1988，13，1： | 1 |
| 16 | Targeting the TLR4/MD - 2 complex for imaging inflammation by SPECT/CT显示文摘 | Smith A Duncan H Duan D | 2011 | J Nucl Med2011，52，1： | 1 |
| 17 | Multiple range and multiple F tests显示文摘 | Duncan D B | 1955 | Biometrics1955，11，： | 1 |
| 18 | The application of infrared mieroimaging for the determination of the distribution, penetration depth,and diffusion profile of methyl centralite and dibutyl phthalate deterrents in nitrocellulose monoperforated propellant 显示文摘 | Louden J D Duncan I A Kelly J | 1993 | Journal of Applied Polymer Science1993，49，： | 1 |
| 19 | Phylogenetic analysis of Phytophthora species based on ITS1 and ITS2 sequences of the ribosomal RNA gene repeat显示文摘 | Cooke D E L Duncan J M | 1997 | Mycological Research1997，101，： | 1 |
| 20 | Construction of a homodimeric dihydrofolate reductase - thymidylate synthase bifunctional en- zyme 显示文摘 | Trujillo M Duncan R Santi D | 1997 | Protein Eng1997，10，5： | 1 |
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