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| 1 | Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma显示文摘BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor progression.Its prognosis is poor and early diagnosis is of utmost importance.This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.METHODS:A hepatoma model was induced in male Sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) expression.Total RNA was purified and transcribed into cDNA by reverse transcription.Fragments of the GPC-3 gene were amplified by nested RT-PCR,and confirmed by sequencing.GPC-3 was analyzed by immunohistochemistry,Western blotting or ELISA.RESULTS:Positive GPC-3 expression showed as brown granule-like staining localized in the cytoplasm.Histological examination of hepatocytes revealed three morphological stages of granule-like degeneration,atypical hyperplasia (precancerous),and cancer formation,with a progressive increase of liver total RNA and GGT expression.The incidence of liver GPC-3 mRNA and GPC-3,and serum GPC-3 was 100%,100% and 77.8% in the HCC group,100%,100%,and 66.7% in the precancerous group,83.3%,83.3%,and 38.9% in the degeneration group,and no expression in the liver or blood of the control group,respectively.There was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475,P<0.05) or liver GPC-3 (r=1.0,P<0.001) or serum GPC-3 (r= 0.994,P<0.001).CONCLUSION:Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC. | Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China (Yao M,Yao DF,Qiu LW,Wu W,Sai WL,Yang JL and Zhang HJ) Department of Oncology,Yancheng First People’s Hospital,Yancheng 224001,China (Bian YZ) Department of Oncology,Second Affiliated Hospital,Nanjing Medical University,Nanjing 210011,China (Zhang CG) | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 51 |
| 2 | Endonasal transsphenoidal removal of tuberculum sellae meningiomas: technical note(英文)显示文摘OBJECTIVE: Tuberculum sellae meningiomas traditionally have been removed through a transcranial approach. More recently, the sublabial transsphenoidal approach has been used to remove such tumors. Here, we describe use of the direct endonasal transsphenoidal approach for removal of suprasellar meningiomas. METHODS: Three women, aged 32, 34, and 55 years, each sought treatment for visual loss and headaches. In each patient, magnetic resonance imaging (MRI) showed a suprasellar mass causing optic chiasmal and optic nerve compression (average size, 2cm x2 cm). All three patients underwent tumor removal via an endonasal approach with | Cook SW Smith Z Kelly DF | 2004 | 中国神经肿瘤杂志2004，2，2： | 36 |
| 3 | 关联用户用途的试车技术显示文摘简单回顾了一些传统的试车方法及背景,着重介绍了一种考虑了用户用途的试车技术,该技术通过比例叠加试验场试验跑道环境下的汽车循环载荷输入,使其符合用户使用环境下的载荷特点,实现两者关联,最后介绍并总结了nCode公司所发展的用户关联试车软件及其特点。 | Ensor,DF 林晓斌 | 1998 | 中国机械工程1998，9，11： | 32 |
| 4 | Microbial community structure of ethanol type fermentation in biohydrogen production显示文摘 | Ren NQ Xing DF Rittmann BE Zhao LH Xie TH Zhao X | 2007 | 中国生物学文摘2007，21，7： | 16 |
| 5 | Endonasal Versus Supraorbital Keyhole Removal of Craniopharynglomas and Tuberoulumsellae Meningiomas显示文摘 | Fatemi N Dusick JR de Paiva Neto MA Malkasian D Kelly DF | 2009 | 中国神经肿瘤杂志2009，7，1： | 12 |
| 6 | 大学生饮酒模式调查分析显示文摘目的分析影响大学生饮酒模式的因素,针对饮酒教育及酒精政策提出建议。方法采用整群抽样方法,选择来自北京和郑州的530名大学生完成有效问卷调查。采用Epidata录入数据,SAS 12.0和R 2.7.2进行数据描述和分析。结果 74.5%的大学生在过去1年内饮过酒,啤酒是饮酒者的主要选择(85.9%),餐馆和家里是饮酒比较频繁的场所,饮用酒多来自同学/朋友及家庭成员提供,25.3%饮酒者并无特别原因饮酒,各有约1/5的饮酒者是出于社交目的或喜欢饮酒的感觉。认为'饮酒有助于社交'或'饮酒促进交流'者、父母及同学/朋友饮酒者、来自农村地区或小城镇者及吸烟者更容易发生饮酒行为(均P<0.05),男生大学生、规律饮酒者更容易遭遇强迫劝酒场面、更易发生醉酒(均P<0.05)。超过一半饮酒者出现酒后脸红、肌肉软弱无力等生理反应,饮酒对被调查大学生产生的社会心理影响依次是记忆丧失(23.9%)、宿醉(14.6%)、做了后悔的事(低于10%)等。结论目前大学生仍保持着一种低风险饮酒模式。大学生饮酒教育应提供关于饮酒利弊的科学信息,使其对饮酒保持一种现实的期望。应制定针对性酒精政策和教育鼓励和支持低风险且满足社交功能的饮酒模式,控制高风险饮酒模式。 | Newman IM Huang Zhaoqing Shell DF 钱玲 | 2014 | 中国社会医学杂志2014，31，2： | 10 |
| 7 | 常规脂蛋白检测和载脂蛋白在心血管病预测中的比较显示文摘总胆固醇和高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)测量值对于心血管病风险评估至关重要,但对于其他脂质用于风险预测的效用一直存在争议。该研究纳入来自英国生物银行(Biobank)的无基线心血管病、未服用他汀类药物、有相关的脂质指标(n=346 686)的受试者。 | Welsh C Celis-Morales CA Brown R Mackay DF Lewsey J Mark PB Gray SR Ferguson LD Anderson JJ Lyall DM Cleland JG Jhund PS Gill J MR Pell JP Sattar N Welsh P 刘青(译) 叶鹏(摘、审校) | 2019 | 中华高血压杂志2019，27，7： | 10 |
| 8 | Interleukin-6 alters the cellular responsiveness to clopidogrel, irinotecan, and Oseltamivir by suppressing the expression of Carboxylesterases HCE1 and HCE2显示文摘 | Yang,j shi,D Yang,DF Song,XL Yan,BF | 2007 | 南京医科大学学报(自然科学版)2007，27，11： | 9 |
| 9 | Byakangelicin induces cytochrome P450 3A4 expression via transactivation of pregnane X receptors in human hepatocytes显示文摘 | Yang, JA Luan, XF Gui, HY Yan, P Yang, DF Song, XL Liu, W Hu, G Yah, BF | 2011 | 南京医科大学学报(自然科学版)2011，31，4： | 8 |
| 10 | Pineal clock gene oscillation is disturbed in Alzheimer's disease, due to functional disconnection from the "master clock"显示文摘 | Wu YH Fischer DF Kalsbcck A Garidou-Boof ML van der Vliet J van Heijningen C Liu RY Zhou JN Swaab DE | 2006 | 中国生物学文摘2006，20，9： | 7 |
| 11 | Application of carbazole compounds in study of hydrocarbon migration显示文摘IN recent years, studies on nitrogen compounds have been widely carried out by overseas schol-are, and achievements have been obtained spec1ally in the application of carbazoles to thestudy of hydrocarbon migration. But in China, few people work on this research area. | Liu, LF Xu, XD Mao, DF Yu, HJ | 1997 | Chinese Science Bulletin1997，42，23： | 6 |
| 12 | Effects of pigment glands and gossypol on somatic cell culture of upland cotton (Gos-sypium hirsutum L.)显示文摘The effects of pigment glands and gossypol on the somatic cell culture of upland cotton were studied, using the materials as follows: three pairs of glanded and gland less upland cotton near isogenic lines, TM-1, and Coker 312. The results showed that the pigment glands and gossypol contents in the explants had great inhibiting effect on the induction and growth of callus in somatic cell culture of upland cotton, and the induction rate of callus and the single callus weight of glandless cotton were much higher than those of their glanded near isogenic lines. It was easier to obtain regeneration plants from glandless cotton than from their glandednear isogenic lines. There was a significant inverse correlation between the gossypol contents in the explants and callus induction rate, with the correlation coefficient of -0.84. The vitro gossypol in the medium had some inhibiting effect on the induction and growth of callus, especially for the gland-less cotton. However, a certain concentration of vitro | Zhu, SJ Ji, DF | 2001 | Chinese Science Bulletin2001，46，23： | 3 |
| 13 | 激光扫描共焦显微镜在内耳形态学研究中的应用显示文摘激光扫描共焦显微镜(LSCM)可直接、无创性地对组织进行连续光学切片,观察同一标本表面和内部结构。为探索LSCM在内耳形态学研究领域的应用前景,应用LSCM结合免疫荧光组织化学双标记技术,观察5只豚鼠耳蜗毛细胞及支持细胞上部构像。通过毒伞素标记毛细胞肌动蛋白纤维,以抗角质蛋白单克隆抗体标记支持细胞。在LSCM下可清楚观察到毛细胞静纤毛、表皮板以及毛细胞顶部无表皮板区;可同时观察到外柱细胞头板、Deiter细胞指状突顶板以及位于网状层下方的Deiter细胞指状突。LSCM为研究Corti器形态增加了一种新的手段。 | 孔维佳 Dolan,DF | 1996 | 中华耳鼻咽喉科杂志1996，31，4： | 3 |
| 14 | 降低驾驶员血液酒精浓度限值对道路交通事故发生率和酒精消费量的影响:一项自然实验显示文摘酒后驾驶是造成全球范围道路交通事故高发生率和死亡率的重要危险因素。降低驾驶者血液酒精浓度是一项常见的公共卫生干预措施,已在许多国家和地区实行。2014年12月5日,苏格兰将驾驶员血液酒精浓度上限值从0.08g/dL降低到0.05g/dL,本研究旨在评估这一政策变化对道路交通伤害和酒精消费的影响。 | Haghpanahan H Lewsey J Mackay DF 李婕(编译) 胡国清(编译) | 2019 | 中华预防医学杂志2019，53，9： | 3 |
| 15 | δ-aminolevulinic acid dehydratase isozymes and lead toxicity显示文摘 | Astrin KH Bishop DF Wetmur JG | 1987 | Ann N Y Acad Sci1987，514，： | 3 |
| 16 | 艾滋病病毒相关性原发性脑淋巴瘤的放疗疗效显示文摘目的:探讨艾滋病病毒相关性原发性脑淋巴瘤放射治疗的疗效。方法:从 1995年1月至 1999年 12月法国巴黎第十二大学Henri Mondor医院放疗科共收治艾滋病病毒相关性原发性脑淋巴瘤病人8例,均为男性,中位年龄为32岁(21~57岁)。8例病人均接受全脑照射30 Gy,后有4例缩野照射原发肿瘤 15~20 Gy,中位照射剂量为 37 .5 Gy。所有病人均未接受化疗。结果:放疗结束时,肿瘤缩小4例,肿瘤无变化 3例,肿瘤增大 1例;病人对急性放射反应耐受性尚好。病人中位生存期为7.1个月(3~19个月)。结论:艾滋病病毒相关性原发性脑淋巴瘤病人的预后差,而放射治疗可在一定程度上延长病人的生存期。 | 曹卡加 Luo DF Kirova Y Le Bourguois JP | 2001 | 癌症2001，20，6： | 3 |
| 17 | Transformed mammalian cells secrete specific proteins and phosphoproteins显示文摘 | Senger DR Wirth DF Hynes RO | | 0，，04： | 2 |
| 18 | Tissue inhibitors of metalloproteinases: Structure regulation and biological functions显示文摘 | Gomez DE Alonso DF Yoshiji H | 1997 | Eur J Cell Biol1997，74，2： | 2 |
| 19 | 胰十二指肠切除术中有血管切除的结果:来自欧洲两个中心对于生存率及无瘤生存的详细因素分析显示文摘该研究的目的是报道欧洲两个肝胰胆外科中心在胰十二指肠切除过程中进行血管切除的经验,并且评估结果及预后因素。1989-2002年两中心共45例因胰腺肿瘤行胰十二指肠切除术的病人,其中男21例、女24例,平均年龄(60±13)岁,(26~82岁);行Whipple's 33例,全胰切除10例,胰体尾、脾脏切除2例。同时切除的血管包括静脉39例、动脉1例、静脉加动脉5例。结果显示:无手术死亡,术后死亡率为2.2%。34例术后病程无大的波动。 | Adham M Mirza DF Chapuis F 黄辉(摘) 邹一平(校) | 2007 | 中华肝胆外科杂志2007，13，9： | 2 |
| 20 | Pharmacokinetics of 9-nitro-20(S) -camptothecin in rats 显示文摘 | Zhong DF Li K Xu JH | 2003 | Acta Pharmacol Sin2003，24，3： | 2 |
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