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| 1 | Novel functional proteins interact with midkine in hepatic cancer cells显示文摘BACKGROUND: Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration and differentiation of various target cells. Midkine plays an important role in tumorigenesis and tumor progression, and is overexpressed in many human malignant tumors. Patients with high tumor midkine expression frequently have a worse prognosis than those with low expression. The present study was designed to investigate the interaction network of midkine in hepatic cancer cells, and to elucidate its role in hepatocellular carcinoma. METHODS: DNA encoding full-length midkine was cloned into pDBLeu vector to serve as bait in yeast two-hybrid screening to identify interacting proteins. Candidate proteins were examined on SC-Leu-Trp-His+3-AT (20 mmol/L) plates and assayed for X-gal activity, then sequenced and classified according to the GenBank. Finally, identified proteins were expressed by the in vitro expression system pCMVTnT, and protein interactions were confirmed by co-immunoprecipitation. RESULTS: Using the yeast two-hybrid system, we found 6 proteins that interacted with midkine: NK-kappa-B inhibitor alpha (I-κ-B-α), Dvl-binding protein naked cuticle 2, granulin, latent active TGF-β binding protein 3, latent active TGF-β binding protein 4, and phospholipid scramblase 1. In vitro co-immunoprecipitation demonstrated that all identified proteins directly interacted with midkine.CONCLUSION: The identification of midkine-interacting proteins in hepatic cancer cells indicates that midkine is a multifunctional factor that may participate in cell migration, differentiation, and proliferation, and is also associated with the multicellular response feedback during the development of hepatocellular carcinoma. | Qiang Yan, Hui-Lian Huang, Xing Yao, Jing Li, Li-Qin Li, Jing Zhong, Li-Shan Min, Li-Cheng Dai and Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery, Huzhou Central Hospital (Yan Q and Yao X) Huzhou Key Laboratory of Molecular Medicine, Affiliated Central Hospital of Huzhou Teachers College (Huang HL, Li J, Li LQ, Zhong J, Min LS and Dai LC), Huzhou 313000, China Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Yan Q and Zheng SS) | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，3： | 7 |
| 2 | Improvement of Fracture Toughness Lanthanum Zirconate显示文摘La2Zr2O7 (LZ) is a promising thermal barrier coating material for the high temperature applications. The fracture toughness and microhardness of nanocrystalline LZ (n-LZ), microcrystalline LZ (m-LZ) and LZ-5mol%8YSZ (LZ-5-8YSZ) composite (8YSZ for zirconia stabilized by 8 mol% ytrria) were studied. The n-LZ had a thermal expansion coefficient of (9.6±0.4)×10 -6 K -1 (200~1000℃) and fracture toughness of (1.98±0.07) MPa·m 1/2 which are obviously higher than those of the m-LZ ( (9.1±0.4)×10 -6 K -1 and (1.40±0.23) MPa·m 1/2, respectively), indicating that nanofication was an efficient way to increase the toughness and thermal expansion coefficient of LZ. The composite LZ-5-8YSZ had a higher fracture toughness ((1.88±0.30) MPa·m 1/2) than LZ, which was close to that of 8YSZ densified by superhigh pressure (SHP). | LI J DAI H LI Q ZHONG X H CAO X Q | 2006 | 材料工程2006，34，5： | 7 |
| 3 | Mutation analysis of autosomal dominant polycystic kidney disease genes in Han Chinese显示文摘 | Zhang S Mei C Zhang D Dai B Tang B Sun T Zhao H Zhou Y Li L Wu Y Wang W Shen X Song J | 2005 | 第二军医大学学报2005，26，8： | 6 |
| 4 | Cyclic adenosine 3',5'-monophosphate induces differentiation of mouse embryonic stem cells into cardiomyocytes显示文摘 | Chen Y Shao JZ Xiang LX Guo J Zhou Q J Yao X Dai LC Lu YL | 2006 | 中国生物学文摘2006，20，7： | 5 |
| 5 | Isolation and characterization of neural stem cells from human fetal striatum显示文摘 | Li X Xu J Bai Y Wang X Dai X Liu Y Zhang J Zou J Shen L Li L | 2006 | 中国生物学文摘2006，20，5： | 3 |
| 6 | Characterization of soil organic matter fractions of tundra soils in Arctic Alaska by carbon 13 nuclear magnetic resonance spectroscopy 显示文摘 | Dai X Y Ping C L Candler R | 2001 | Soil Science Society of America Journal2001，65，： | 2 |
| 7 | 收缩压和舒张压变异性对脑卒中患者血管事件的不同影响显示文摘较高的血压变异性(blood pressure variability,BPV)是心血管病的危险因素。然而,在引发不同的血管事件时,收缩压变异性(systolic blood pressure variability,SBPV)和舒张压变异性(diastolic blood pressure variability,DBPV)是否存在不同作用仍不清楚。本研究旨在探讨SBPV或DBPV对急性缺血性脑卒中(acute ischaemic stroke,IS)或短暂性脑缺血发作(transient ischaemic attack,TIA)患者血管事件的作用是否存在区别。 | 周卫(译) 袁源(摘) 叶鹏(审校) Dai L Cheng A Hao X Xu J Zuo Y Wang A Meng X Li H Wang Y Zhao X Wang Y | 2020 | 中华高血压杂志2020，28，11： | 2 |
| 8 | Targeting tumours by adoptive transfer of immune cells显示文摘 | Macary P A Too C T Dai X | 2006 | Clin Exp Pharmacol Physiol2006，33，56： | 1 |
| 9 | Dual effects of intrathecal BAM22 on nociceptive responses in acute and persistent pain-potential function of a novel receptor 显示文摘 | Hong Y Dai P Jiang J Zeng X | 2004 | Br J Pharmacol2004，141，： | 1 |
| 10 | A feature-based image registration algorithm using improved chain-code representation combined with invariant moments 显示文摘 | Dai X Khorram S | 1999 | IEEE Transactions on Geoseienee and Remote Sensing(S0196-2892)1999，37，5： | 1 |
| 11 | A Nonlinear Conjugate Gradient with a Strong Global Convergence Property 显示文摘 | Dai Y H Yuan Y X | 2000 | SIAM Journal of Optimization2000，10，： | 1 |
| 12 | Soil nutrient factors related to salidroside production of rhodiola sachalinensis distributed in chang bai mountain显示文摘 | YAN X F WU S X WANG Y SHANG X H DAI S J | 2004 | Environmental and Experimental Botsny2004，52，： | 1 |
| 13 | dsRNA-mediated in-nate immunity of epidermal keratinocytes显示文摘 | Tohyama M Dai X Sayame K | 2005 | Biochem Bio-phys Res Commun2005，335，2： | 1 |
| 14 | MIMO system invertibility and de- coupling control strategies based on ANN ath-order inversion显示文摘 | DAI X HE D ZHANG X | 2001 | IEE Proceedings-Control Theory and Applications2001，148，2： | 1 |
| 15 | Quasi-synchronous sampling algorithm and its applications显示文摘 | X Z Dai | 1993 | IEEE Trans1993，43，2： | 1 |
| 16 | Toxic effects of Zn2 + , Cu2 + , Cd2 + and NH3 on Chinese prawn显示文摘 | Zang W L Xu X C Dai X L | 1993 | Chinese J Oceanal Limol1993，11，3： | 1 |
| 17 | Synthesis and characterization of indodicarbocyanines显示文摘 | Dai Z F Peng B X | 1998 | Dyes and Pigments1998，36，2： | 1 |
| 18 | The physics and phenomenology of one atmosphere uniform glow discharge plasma (OAUGDP (TM)) reactors for surface treatment applications 显示文摘 | Roth J R Rahel J Dai X | 2005 | J Phys D: Appl Phys2005，38，4： | 1 |
| 19 | A combined HPLC-PDA and HPLC-MS method for quantitative and qualitative analysis of 10 major constituents in the traditional Chinese medicine Zuo Gui Wan 显示文摘 | LIANG X ZHANG X DAI W | 2009 | J Pharm Biomed Anal2009，49，4： | 1 |
| 20 | TSR and sour gas accumula-tion: A case study in the Sichuan basin,SW China显示文摘 | Zhang S C Zhu G Y Dai J X | 2005 | Geochemicaet Cosmochimica Acta2005，69，10： | 1 |
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