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| 1 | Novel functional proteins interact with midkine in hepatic cancer cells显示文摘BACKGROUND: Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration and differentiation of various target cells. Midkine plays an important role in tumorigenesis and tumor progression, and is overexpressed in many human malignant tumors. Patients with high tumor midkine expression frequently have a worse prognosis than those with low expression. The present study was designed to investigate the interaction network of midkine in hepatic cancer cells, and to elucidate its role in hepatocellular carcinoma. METHODS: DNA encoding full-length midkine was cloned into pDBLeu vector to serve as bait in yeast two-hybrid screening to identify interacting proteins. Candidate proteins were examined on SC-Leu-Trp-His+3-AT (20 mmol/L) plates and assayed for X-gal activity, then sequenced and classified according to the GenBank. Finally, identified proteins were expressed by the in vitro expression system pCMVTnT, and protein interactions were confirmed by co-immunoprecipitation. RESULTS: Using the yeast two-hybrid system, we found 6 proteins that interacted with midkine: NK-kappa-B inhibitor alpha (I-κ-B-α), Dvl-binding protein naked cuticle 2, granulin, latent active TGF-β binding protein 3, latent active TGF-β binding protein 4, and phospholipid scramblase 1. In vitro co-immunoprecipitation demonstrated that all identified proteins directly interacted with midkine.CONCLUSION: The identification of midkine-interacting proteins in hepatic cancer cells indicates that midkine is a multifunctional factor that may participate in cell migration, differentiation, and proliferation, and is also associated with the multicellular response feedback during the development of hepatocellular carcinoma. | Qiang Yan, Hui-Lian Huang, Xing Yao, Jing Li, Li-Qin Li, Jing Zhong, Li-Shan Min, Li-Cheng Dai and Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery, Huzhou Central Hospital (Yan Q and Yao X) Huzhou Key Laboratory of Molecular Medicine, Affiliated Central Hospital of Huzhou Teachers College (Huang HL, Li J, Li LQ, Zhong J, Min LS and Dai LC), Huzhou 313000, China Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Yan Q and Zheng SS) | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，3： | 7 |
| 2 | Improvement of Fracture Toughness Lanthanum Zirconate显示文摘La2Zr2O7 (LZ) is a promising thermal barrier coating material for the high temperature applications. The fracture toughness and microhardness of nanocrystalline LZ (n-LZ), microcrystalline LZ (m-LZ) and LZ-5mol%8YSZ (LZ-5-8YSZ) composite (8YSZ for zirconia stabilized by 8 mol% ytrria) were studied. The n-LZ had a thermal expansion coefficient of (9.6±0.4)×10 -6 K -1 (200~1000℃) and fracture toughness of (1.98±0.07) MPa·m 1/2 which are obviously higher than those of the m-LZ ( (9.1±0.4)×10 -6 K -1 and (1.40±0.23) MPa·m 1/2, respectively), indicating that nanofication was an efficient way to increase the toughness and thermal expansion coefficient of LZ. The composite LZ-5-8YSZ had a higher fracture toughness ((1.88±0.30) MPa·m 1/2) than LZ, which was close to that of 8YSZ densified by superhigh pressure (SHP). | LI J DAI H LI Q ZHONG X H CAO X Q | 2006 | 材料工程2006，34，5： | 7 |
| 3 | Cyclic adenosine 3',5'-monophosphate induces differentiation of mouse embryonic stem cells into cardiomyocytes显示文摘 | Chen Y Shao JZ Xiang LX Guo J Zhou Q J Yao X Dai LC Lu YL | 2006 | 中国生物学文摘2006，20，7： | 5 |
| 4 | Immobilization of hemoglobin on zirconium dioxide nanoparticles for preparation of a novel hydrogen peroxide biosensor 显示文摘 | Liu S Q Dai Z H Chen H Y | 2004 | Biosens Bioelectron2004，19，： | 1 |
| 5 | Adenovirus-mediated gene trans- fer to healing tendon--enhanced efficiency using a gelatin sponge显示文摘 | Dai Q Manfield L Wang Y | 2003 | J Orthop Res2003，21，4： | 1 |
| 6 | A short note on quenching phenomena for semilinear parabolic equations 显示文摘 | Dai Q Y Gu Y G | 1997 | J Differential Equations1997，137，： | 1 |
| 7 | Femoral neck frac-ture fixation:A biomechanical study of two cannulatedscrew placement techniques显示文摘 | Booth KC Donaldson TK Dai Q | 1998 | Orthopedics1998，21，11： | 1 |
| 8 | Performance simula- tion of high ahitude scientific balloons 显示文摘 | DAI Q M FANG X D LI X J | 2012 | Advances in Space Research2012，49，： | 1 |
| 9 | Mechanical properties and microstructure of nanocrystalline nickel-carbon nanotube composites produced by electrodeposition 显示文摘 | DAI P Q XU W C HUANG Q Y | 2008 | Materials Science and Engineering: A2008，，： | 1 |
| 10 | Preparation of porous carbon spheres from porous starch显示文摘 | Wang H Dai Q Li Q | 2009 | Solid State Ionics2009，180，2627： | 1 |
| 11 | Preparation of monodisper- se poly (methyl methacrylate) particles by radiation-induced dis- persion polymerization using vinyl terminus polysiloxane mac- romonomer as a polymerizable stabilizer显示文摘 | DAI Q WU D Z ZHANG Z | 2003 | Polymer2003，44，1： | 1 |
| 12 | Preparation of monodisperse poly (methyl methacrylate) particles by radiation-induced dispersion polymerization using vinyl terminus polysiloxane macromonomer as a polymerizable stabilizer显示文摘 | Dai Q Wu D Z Zhang Z | | 0，，01： | 1 |
| 13 | IrrE, a global regulator of extreme radiation resistance inDeinococcus radiodurans, enhances salt tolerance in Escherichia coli and Brassica napus显示文摘 | Pan J Wang J Zhou Z Yan Y Zhang W Lu W Ping S Dai Q Yuan M Feng B Hou X Zhang Y Ma R Liu T Feng L Wang L Chen M Lin M | 2009 | PloS One2009，4，2： | 1 |
| 14 | Using bulked extremes and recessive class to map genes for photoperiod - sensitive genic male sterility in rice 显示文摘 | Zhang Q F Shen B Z Dai X K | 1994 | Proc Natl Acad Sci USA1994，91，18： | 1 |
| 15 | IL-27 inhibits HIV-1infection in human macrophages by down-regulating host factor SPTBN1 during monocyte to macrophage differentiation显示文摘 | Dai L Lidie KB Chen Q | 2013 | Journal of Experimental Medicine2013，11，： | 1 |
| 16 | Synthesis of Bi6Mo2015sub-microwires via a molten salt method and enhancing thephotocatalytic reduction of CO2 into solar fuel through tuning the surface oxide vacancies by simple post-heating treatment显示文摘 | Li P Zhou Y Tu W Wang R Zhang C Liu Q Li H Li Z Dai H Wang J Yan S Zou Z | 2013 | CrystEngComm2013，15，46： | 1 |
| 17 | Performance of K and Ni substituted La1-xKxCo1-yNiyO3-δ perovskite catalysts used for soot combustion, NOx storage and simultaneous NOx- soot removal 显示文摘 | Li Z Q Meng M Dai F F | 2012 | Fuel2012，93，： | 1 |
| 18 | Expression pattern genomic struc- ture, and promoter analysis of the gene encoding stilbene synthase from Chinese wild Vitis pseudoreticulata显示文摘 | XU W R YU Y H ZHOU Q DING J H DAI L M XIE X Q XU Y ZHANG C H WANG Y J | 2011 | Journal of Experimen- tal Botany2011，62，8： | 1 |
| 19 | Do markets prefer open or proprietary standards for XML standardization? an event study显示文摘 | Aggarwal N Dai Q Z Walden E A | 2006 | International Journal of Electronic Commerce2006，，11： | 1 |
| 20 | Field Emission From Nanotube Bundle Emitters at Low Fields显示文摘 | Wang Q H Corrigan T D Dai J Y | 1997 | Appl Phys Lett1997，70，24： | 1 |
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