|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Relative quantification of phosphoproteomic changes in grapevine (Vitis vinifera L.) leaves in response to abscisic acid显示文摘In a previous transcriptomic analysis,abscisic acid(ABA)was found to affect the abundance of a number of transcripts in leaves of Cabernet Sauvignon grapevines with roots that had been exposed to 10μM ABA for 2 h.Other work has indicated that ABA affects protein abundance and protein phosphorylation as well.In this study we investigated changes in protein abundance and phosphorylation of Cabernet Sauvignon grapevine leaves.Protein abundance was assessed by both label-free and isobaric-label quantitive proteomic methods.Each identified common proteins,but also additional proteins not found with the other method.Overall,several thousand proteins were identified and several hundred were quantified.In addition,hundreds of phosphoproteins were identified.Tens of proteins were found to be affected in the leaf after the roots had been exposed to ABA for 2 h,more than half of them were phosphorylated proteins.Many phosphosites were confirmed and several new ones were identified.ABA increased the abundance of some proteins,but the majority of the proteins had their protein abundance decreased.Many of these proteins were involved in growth and plant organ development,including proteins involved in protein synthesis,photosynthesis,sugar and amino-acid metabolism.This study provides new insights into how ABA regulates plant responses and acclimation to water deficits. | Supakan Rattanakan Iniga George Paul A Haynes Grant R Cramer | 2016 | Horticulture Research2016，3，1： | 3 |
| 2 | Cholangiocarcinoma, gone without the Wnt?显示文摘Cholangiocarcinoma(CCA) is a relatively rare malignancy of the intra- or extra-hepatic bile ducts that is classified according to its anatomical localization as intrahepatic, perihilar or distal. Overall, CCA has a dismal prognosis due to typical presentation at an advanced irresectable stage, lack of effective non-surgical treatments, and a high rate of disease recurrence. CCA frequently arises on a background of chronic liver inflammation and cholestasis. Chronic inflammation is accompanied by enhanced cell turnover with generation of additional inflammatory stimuli, and a microenvironment rich in pro-inflammatory mediators and proliferative factors that enable accumulation of mutations, transformation and expansion of mutated cells. A recent study by Boulter et al implicates the Wnt signaling cascade in cholangiocarcinogenesis. Wnt ligands Wnt7 B and Wnt10 A were found to be highly overexpressed in human CCA tissue. Wnt7 B protein was present throughout the tumor stroma, and often co-localized with a subset of CD68+ macrophages. To address in a direct manner whether Wnt signaling is engaged in development of CCA, Boulter et al explored the Wnt signaling pathway in an experimental model that recapitulates the multi-stage progression of human CCA.Wnt ligands found to be elevated in human CCA were also upregulated during the course of CCA development following thioacetamide treatment. Wnt10 a increased during the(pre-cancerous) regenerative phase, while Wnt7 b induction paralleled tumor growth. Along with upregulation of target genes, the findings demonstrate that the canonical Wnt pathway is progressively activated during cholangio-carcinogenesis. Macrophage depletion,eliminating a major source of Wnt7 b, prevented activation of the canonical Wnt cascade, and resulted in reduced number and volume of tumors in this model. Moreover,specific inhibitors of the canonical Wnt pathway(ICG-001 and C-59) caused reduction of tumor area and number,in xenograft and thioacetamide models of CCA. The aggregated findings show that experimental, and presumably human CCA, is a Wnt-driven tumor. Modulation of Wnt signaling, alone or in combination with surgicalor chemotherapy approaches, holds promise in the management of this fatal malignancy. | Anne T R Noll Thorsten Cramer Steven W M Olde Damink Frank G Schaap | 2016 | World Journal of Hepatology2016，8，26： | 3 |
| 3 | A rapid dehydration leaf assay reveals stomatal response differences in grapevine genotypes显示文摘A simple and reliable way of phenotyping plant responses to dehydration was developed.Fully-developed leaves were detached and placed in a closed plastic box containing a salt solution to control the atmospheric water potential in the container.Three hours of dehydration(weight loss of the leaf)was optimal for measuring changes in stomatal response to dehydration.Application of the plant hormone abscisic acid(ABA)prior to leaf detachment decreased the amount of water loss,indicating that the assay was able to detect differences based on a stomatal response to dehydration.Five different Vitis genotypes(V.riparia,V.champinii,V.vinifera cv.Shiraz,V.vinifera cv.Grenache and V.vinifera cv.Cabernet Sauvignon)with known differences in drought tolerance were screened for their dehydration response and the results obtained corresponded to previous reports of stomatal responses in the vineyard.Significant differences in stomatal density along with differences in the amount and rate of water lost indicate differences in dehydration sensitivity among the genotypes screened.Differences in stomatal response to ABA were also detected.Shiraz had the lowest stomatal density and the highest ABA sensitivity among the genotypes screened,yet Shiraz lost the most amount of water,indicating that it was the least sensitive to dehydration.Despite having the highest stomatal density and intermediate stomatal sensitivity to ABA,V.riparia lost the smallest amount of water,indicating that it was the most sensitive to dehydration.The assay presented here represents a simple and reliable phenotyping method for plant responses to leaf dehydration. | Daniel W Hopper Ryan Ghan Grant R Cramer | 2014 | Horticulture Research2014，1，1： | 2 |
| 4 | Modelling isotope fractionation during primary cracking of natural gas: a reation kinetic approach 显示文摘 | CRAMER B KROSS B M LITTKE R | 1998 | Chemical Geology1998，149，34： | 1 |
| 5 | Sclerosing of recurrent lymphangioma using OK-432显示文摘 | Mikhail M Kennedy R Cramer B | 1995 | J Pediatr Surg1995，30，8： | 1 |
| 6 | Modelling isotope fractionation during primary cracking of natural gas:a reaction kinetic approach显示文摘 | Cramer B Krooss B M Littke R | 1998 | Chemical Geology1998，149，： | 1 |
| 7 | Measuring special effects显示文摘 | Cramer W R Gabel P W | 2001 | Eu- ropean Coatings Journal2001，，78： | 1 |
| 8 | Signature scheme based on the strong RSA assumption显示文摘 | CRAMER R SHOUP V | 2000 | ACM Transactions on Information and System Security2000，3，3： | 1 |
| 9 | Displacement of Ca2+by Na+from the plasmalemms of root cells显示文摘 | Cramer G R Luchli André Polito V S | 1985 | Plant Physiol1985，79，1： | 1 |
| 10 | Displacement of Ca^2 + by Na^+ from the plasmalemma of root cells 显示文摘 | Cramer G R Lauchli A Polito V S | 1985 | Plant Physiol1985，79，： | 1 |
| 11 | Quantitative plant pro- teomics显示文摘 | BLINDSCHEDLER L V CRAMER R | 2011 | Proteomics2011，11，4： | 1 |
| 12 | Salinity-calciumn interactions on root growth and osmotic adjustment of two corn cultivars differing in salt tolerance显示文摘 | Cramer G R Abdel B R Seeman J R | 1990 | Joumal of Plant Nutrition1990，13，： | 1 |
| 13 | Design and analysis of practical public-key encryption schemes secure against adaptive chosen ciphertext attack显示文摘 | Cramer R Shoup V | 2003 | SIAM Journal on Computing2003，33，1： | 1 |
| 14 | Tissue-specific mRNA expression profiling in grape berry tissues显示文摘 | GRIMPLET J DELUC L G TILLETT R L WHEATLEY M D SCHLAUCH K A CRAMER G R CUSHMAN J C | 2007 | BMC Genomics2007，8，： | 1 |
| 15 | Novel tetranortriterpenoids with insect antifeeding activity from neem oil 显示文摘 | Kraus W Cramer R | 1981 | Liebigs Ann Chem1981，1981，1： | 1 |
| 16 | Evaluation of an ultra-wideband propagation channel显示文摘 | CRAMER R J M SCHOLTZ R A WIN M Z | 2002 | IEEE Trans on Antenna and Propagation2002，50，5： | 1 |
| 17 | Modelling isotope fractionation during pri mary cracking of natural gas: a reaction kinetic approach显示文摘 | Cramer B Krooss B M Littke R | 1998 | Chemical Geology1998，149，34： | 1 |
| 18 | Calcineurin traget crzA regulates conidial germination,hyphal growth, and pathogenesis of Aspergillus fumigatus 显示文摘 | Cramer R A Perfect B Z Pinchai N | 2008 | Eukaryotic Cell2008，7，7： | 1 |
| 19 | Randomized controlled trial of pulsating cupping (pneumatic pulsation therapy)for chronic neck pain显示文摘 | Cramer H Lauche R Hohmann C | | 0，，06： | 1 |
| 20 | Natural ecosystems:pattern and process in relation to local and landscape diversity in southwestern Australian woodlands显示文摘 | Hobbs R Cramer V A | 2003 | Plant and Soil2003，257，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员