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| 1 | Up-regulation of tumor necrosis factor-a pathway survival genes and of the receptor TNFR2 in gastric cancer显示文摘BACKGROUND Gastric carcinogenesis can be induced by chronic inflammation triggered by Helicobacter pylori(H. pylori) infection. Tumor necrosis factor(TNF)-α and its receptors(TNFR1 and TNFR2) regulate important cellular processes, such as apoptosis and cell survival, and the disruption of which can lead to cancer. This signaling pathway is also modulated by microRNAs(miRNAs), altering gene expression.AIM To evaluate the mRNA and miRNAs expression involved in the TNF-α signaling pathway in gastric cancer(GC) tissues and its relationship.METHODS Quantitative polymerase chain reaction(qPCR) by TaqMan? assay was used to quantify the RNA transcript levels of TNF-α signaling pathway(TNF, TNFR1,TNFR2, TRADD, TRAF2, CFLIP, NFKB1, NFKB2, CASP8, CASP3) and miRNAs that targets genes from this pathway(miR-19 a, miR-34 a, miR-103 a, miR-130 a,miR-181 c) in 30 GC fresh tissue samples. Molecular diagnosis of H. pylori was performed by nested PCR for gene HSP60. A miRNA:mRNA interaction network was construct using Cytoscape v3.1.1 from the in silico analysis performed using public databases.RESULTS Up-regulation of cellular survival genes as TNF, TNFR2, TRADD, TRAF2, CFLIP,and NFKB2, besides CASP8 and miR-34 a was observed in GC tissues, whereas mediators of apoptosis such as TNFR1 and CASP3 were down-regulated. When the samples were stratified by histological type, the expression of miR-103 a and miR-130 a was significantly increased in the diffuse-type of GC compared to the intestinal-type. However, no influence of H. pylori infection was observed on the expression levels of mRNA and miRNAs analyzed. Moreover, the miRNA:mRNA interaction network showed several interrelations between the miRNAs and their target genes, highlighting miR-19 a and miR-103 a, which has as predicted or validated target a large number of genes in the TNF-α pathway,including TNF, TNFR1, TNFR2, CFLIP, TRADD, CASP3 and CASP8.CONCLUSION Our findings show that cell survival genes mediated by TNF/TNFR2 binding is up-regulated in GC favoring its pro-tumoral effect, while pro-apoptotic genes as CASP3 and TNFR1 are down-regulated, indicating disbalance between apoptosis and cell proliferation processes in this neoplasm. This process can also be influenced by an intricate regulatory network of miRNA:mRNA. | Ana Flavia Teixeira Rossi Julia Cocenzo Contiero Fernanda da Silva Manoel-Caetano Fabio Eduardo Severino Ana Elizabete Silva | 2019 | World Journal of Gastrointestinal Oncology2019，11，4： | 5 |
| 2 | Rhamnolipid Production by Pseudomonas aeruginosa LBI Growing on Soapstock as the Sole Carbon Source 显示文摘 | BENINCASA M CONTIERO J MANRESA M A | 2002 | J Food Eng2002，54，4： | 1 |
| 3 | The use of response surface methodology in optimization of lactic acid production: focus on medium supplementation, temperature and pH control显示文摘 | de LIMA C J B COELHO L F CONTIERO J | 2010 | Food Technology and Biotechnology2010，48，2： | 1 |
| 4 | Rhamnolipid production by Psedudomonas aeruginosa LBI growing on soapstock as the sole carbon source显示文摘 | Benincasa M Contiero J Manresa M A | 2002 | Journal of Food Engineering2002，54，： | 1 |
| 5 | Electrophoretic analysis of non human primates hair keretin显示文摘 | Contiero E | 1996 | Forensic Science In ternational1996，83，： | 1 |
| 6 | Prolonged observation and modelling of milk coagulation,curd firming,and syneresis显示文摘 | BITTANTE G CONTIERO B CECCHINATO A | 2013 | International Dairy Journal2013，29，： | 1 |
| 7 | Production, purification and characterization of an extravellular inulinase from Kluyveromyc marxianus var buigaricus 显示文摘 | Kushi Rt Monti R Contiero J | 2002 | Microbiol Biotechnol2002，25，2： | 1 |
| 8 | Rhamnolipid production by Pseudomonas aeruginosa LBI growing on soapstock as the sole carbon source 显示文摘 | Benincasa M Contiero J Manresa M A | 2002 | Journal of Food Engineering2002，54，4： | 1 |
| 9 | Does stocking density modify affective state in pigs as assessed by cognitive bias, behavioural and physiological parameters? 显示文摘 | SCOLLO A GOTTARDO F CONTIERO B | 2014 | Appl Anim Behav Sci2014，，153： | 1 |
| 10 | Effects of mutations in ace- tate metabolism on hlgh-cell-density growth of Escherichia coil 显示文摘 | Contiero J Beatty C Kumari S | 2000 | J Ind Microb Biotechnol2000，24，6： | 1 |
| 11 | Rhamnolipid surfactants: an update on the general aspects of these remarkable biomole- cules显示文摘 | Nitsehke M Costa S G Contiero J | 2005 | Biotechnol Prog2005，21，6： | 1 |
| 12 | Comparison with manual registration reveals satisfactory completeness and effieieney of a computerized cancer registration system显示文摘 | Paolo Contiero A Andrea Tittarelli A Maghini A | 2008 | J Biomed Inform2008，41，： | 1 |
| 13 | Structure, properties and applications of rhamnolipids produced by Pseudomonas aeruginosa L2-1 from cassava wastewater显示文摘 | Siddhartha G.V.A.O. Costa Marcia Nitschke Fran?ois Lépine Eric Déziel Jonas Contiero | 2010 | Process Biochemistry2010，，9： | 1 |
| 14 | Trace element determination in humans 显示文摘 | Folin M Contiero E Vaselli GM | 1991 | Biological Trace Element Research1991，31，2： | 1 |
| 15 | Genetic evaluation of type traits in hypertrophic Piemontese cows显示文摘 | Mantovani R Cassandro M Contiero B | 2010 | Journal of Animal Science2010，88，11： | 1 |
| 16 | Trace element determination in humans 显示文摘 | Folin M Contiero E Vaselli GM | 1991 | Biological Trace Element Reasearch1991，31，2： | 1 |
| 17 | Depletion of chloramphenicol in trout after a hypothetic therapeutic treatment 显示文摘 | G Biancotto L Contiero C Benetti | 2008 | Analytica Chimica Acta2008，08，： | 1 |
| 18 | Structure and Applications of a Rhamnolipid Surfactant Produced in Soybean Oil Waste显示文摘 | Marcia Nitschke Siddhartha G. V. A. O. Costa Jonas Contiero | 2010 | Applied Biochemistry and Biotechnology2010，，7： | 1 |
| 19 | Structure and applications of a rhamnolipid surfactant produced in soybean oil waste显示文摘 | Nitschke M Costa S G Contiero J | | 0，，07： | 1 |
| 20 | Glycerol: A Promising and Abundant Carbon Source for Industrial Microbiology显示文摘 | Gervasio D S P Matthias M Contiero J | 2009 | Biotechnology Advances2009，27，1： | 1 |
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