|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Rat model of cholelithiasis with human gallstones implanted in cholestasis-induced virtual gallbladder显示文摘AIM:To facilitate translational research on cholelithiasis,we have developed a rat model of human gallstones by exploiting the unique biliopancreatic features of this species.METHODS:Under anesthesia,16 adult rats of equal genders underwent two times of abdominal surgery.First,their common bile duct(CBD)was ligated to cause cholestasis by total biliary obstruction(TBO).On day 0,1,3,7,14,21 and 28 after TBO,magnetic resonance imaging(MRI)was conducted to monitor the dilatation of the CBD,and blood was sampled to analyze total serum bilirubin(TSB).Secondly,on day 30,the abdomen was re-opened and gallstone(s)collected from human patients were implanted in the dilated CBD asa virtual gallbladder(VGB),which was closed by suture ligation.This rat cholelithiasis model was examined by MRI,clinical observation,microcholangiography and histology.RESULTS:All rats survived two laparotomies.After ligation,the CBD was dilated to a stable size of 4 to30 mm in diameter on day 21-28,which became a VGB.The rats initially showed signs of jaundice that diminished over time,which paralleled with the evolving TSB levels from 0.6±0.3 mg/d L before ligation,through a peak of 10.9±1.9 mg/dL on day 14,until a nearly normalized value after day 28.The dilated CBD with thickened wall allowed an incision for implantation of human gallstones of 1-10 mm in diameter.The rat cholelithiasis was proven by in vivo MRI and postmortem microcholangiography and histomorphology.CONCLUSION:A rat model cholelithiasis with human gallstones has been established,which proves feasible,safe,reliable,nontoxic and cost-effective.Given the gallstones of human origin,applications of this model may be of help in translational research such as optical detection and lysis of gallstones by systemic drug administration. | Marlein Miranda Cona Yewei Liu Ting Yin Yuanbo Feng Feng Chen Stefaan Mulier Yue Li Jian Zhang Raymond Oyen Yicheng Ni | 2016 | World Journal of Methodology2016，6，2： | 7 |
| 2 | An overview of translational(radio)pharmaceutical research related to certain oncological and non-oncological applications显示文摘Translational medicine pursues the conversion of scientific discovery into human health improvement.It aims to establish strategies for diagnosis and treatment of diseases.Cancer treatment is difficult.Radiopharmaceutical research has played an importantrole in multiple disciplines,particularly in translational oncology.Based on the natural phenomenon of necrosis avidity,Onco Ci Dia has emerged as a novel generic approach for treating solid malignancies.Under this systemic dual targeting strategy,a vascular disrupting agent first selectively causes massive tumor necrosis that is followed by iodine-131 labeled-hypericin(123IHyp),a necrosis-avid compound that kills the residual cancer cells by crossfire effect of beta radiation.In this review,by emphasizing the potential clinical applicability of Onco Ci Dia,we summarize our research activities including optimization of radioiodinated hypericin Hyp preparations and recent studies on the biodistribution,dosimetry,pharmacokinetic and,chemical and radiochemical toxicities of the preparations.Myocardial infarction is a global health problem.Although cardiac scintigraphy using radioactive perfusion tracers is used in the assessment of myocardial viability,searching for diagnostic imaging agents with authentic necrosis avidity is pursued.Therefore,a comparative study on the biological profiles of the necrosis avid 123I-Hyp and the commercially available 99mTc-Sestamibi was conducted and the results are demonstrated.Cholelithiasis or gallstone disease may cause gallbladder inflammation,infection and other severe complications.While studying the mechanisms underlying the necrosis avidity of Hyp and derivatives,their naturally occurring fluorophore property was exploited for targeting cholesterol as a main component of gallstones.The usefulness of Hyp as an optical imaging agent for cholelithiasis was studied and the results are presented.Multiple uses of automatic contrast injectors may reduce costs and save resources.However,cross-contaminations with bloodborne pathogens of infectious diseases may occur.We developed a radioactive method for safety evaluation of a new replaceable patient-delivery system.By mimicking pathogens with a radiotracer,we assessed the feasibility of using the system repeatedly without septic risks.This overview is deemed to be interesting to those involvedin the related fields for translational research. | Marlein Miranda Cona Peter de Witte Alfons Verbruggen Yicheng Ni | 2013 | World Journal of Methodology2013，3，4： | 2 |
| 3 | Plant amine oxidases 'on the move': An update显示文摘 | Angelini R Cona A Federico R | 2010 | Plant Physiology and Biochemistry2010，48，7： | 1 |
| 4 | Polymine oxidase,a hydrogen peroxide-producing enzyme,is upregulated by light and down-regulated by auxin in the outer tissues of the maize mesocoty显示文摘 | Cona A Cenci F Cervelli M | 2003 | Plant Physiol2003，131，： | 1 |
| 5 | The polyamines and their catabolic products are significant players in the turnover of nitrogenous molecules in plants显示文摘 | P.N. Moschou J. Wu A. Cona P. Tavladoraki R. Angelini K.A. Roubelakis-Angelakis | 2012 | Journal of Experimental Botany2012，，14： | 1 |
| 6 | A dual- targeting anticancer approach: soil and seed principle 显示文摘 | Li J Sun Z Zhang J Shao H Cona MM Wang H | 2011 | Radiolo- gy2011，260，3： | 1 |
| 7 | A safety study on single intravenous dose of tetrachloro-diphenyl glycoluril dissolved in dimethyl sulphoxide (DMSO)显示文摘 | Cona M M Li J Chert F | 2013 | Xenobiotica2013，1，2： | 1 |
| 8 | Electrophysiological Correlates of Strategic Monitoring in Event Based and Time Based Prospective Memory显示文摘 | Cona G Arcara G Tarantino V Bisiacchi P S | 2012 | PLoS One2012，7，31： | 1 |
| 9 | Agmrelated Differences in the Neural Correlates of Remembering Time based In tentions显示文摘 | Cona G Arcara G Tarantino V Bisiacchi P S | 2012 | Neuropsychologia2012，50，11： | 1 |
| 10 | Functions of amine oxidases in plant development and defense 显示文摘 | CONA A REA A ANGELINi R | 2006 | Trends in Plant Science2006，11，2： | 1 |
| 11 | Polyamine oxidase,a hydrogen Peroxide-Producing enzyme,15 uP-regulated by Iight and down-regulated by auxin in the outer tissues of the maize mesocotyl显示文摘 | Cona A Cenci F Francesco | 2003 | Plant Physiol2003，，3： | 1 |
| 12 | The polyamines and their catabolic products are significant players in the turnover of nitrogenous molecules in plants显示文摘 | MOSCHOU P N WU J CONA A | 2012 | Journal of Experimental Botany2012，63，14： | 1 |
| 13 | Diet of a fossrial rodent ( Oct- odontidae), abovegroundfood availability, and changes related to cattle grazing in the Central M (Arentina) 显示文摘 | Rosi M Puig S Cona M | 2009 | Journal of Arid Environments2009，73，3： | 1 |
| 14 | Cellular re-distribution of flavin-containing polyamine oxidase in differentiating root and mesocotyl of Zea mays L. seedlings显示文摘 | Alessandra Cona Sandra Moreno Francesco Cenci Rodolfo Federico Riccardo Angelini | 2005 | Planta2005，，2： | 1 |
| 15 | PoLyamine oxidase, 15 uP-reguLated by light and down-reguLated by auxin in the outer tissues of the maize mesocotyl显示文摘 | Cona A Cenci F Francesco | 2003 | PLant Physiol2003，，3： | 1 |
| 16 | A review on various targeted anti- cancer therapies显示文摘 | Li J Chen F Cona MM | 2012 | Target Oncol2012，7，1： | 1 |
| 17 | Contingency constrained optimal reactive power flow procedures for voltage control in planning and operation显示文摘 | Cona B Losignore N Marannino P | 1995 | IEEE Trans on Power Systems1995，10，2： | 1 |
| 18 | Differential effects of emotional cues on components of prospec- tive memory:An ERP study 显示文摘 | CONA G KLIEGEL M BISIACCHI P | 2015 | Frontiers in HumanNeuroscience2015，9，10： | 1 |
| 19 | A safety study on single intravenous dose of tetrachloro-diphenyl glycolurildissolved in dimethyl sulphoxide(DMSO)显示文摘 | Cona M M Li J Chen F | 2013 | Xenobiotica2013，1，2： | 1 |
| 20 | Long-term drought stress induces structural and functional reorganizatio of photosystⅡ显示文摘 | GIARDI M T CONA A GEIKEN B | 1996 | Planta1996，199，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员