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| 1 | Preventative effects of a probiotic, Lactobacillus salivarius ssp. salivarius, in the TNBS model of rat colitis显示文摘AIM: To investigate the intestinal anti-inflammatory effect and mechanism of a probiotic Lactobacillus sakuvarius ssp.salivariusCECT5713 in the TNBS model of rat colitis.METHODS: Female Wistar rats (180-200 g) were used in this study. A group of rats were administered orally the probiotic L. salivarius ssp. salivarius (5×108 CFU suspended in 0.5 mL of skimmed milk) daily for 3 wk. Two additional groups were used for reference, a non-colitic and a control colitic without probiotic treatment, which received orally the vehicle used to administer the probiotic. Two weeks after starting the experiment, the rats were rendered colitic by intracolonic administration of 10 mg of TNBS dissolved in 0.25 mL of 500 mL/L ethanol. One week after colitis induction, all animals were killed and colonic damage was evaluated both histologically and biochemically. The biochemical studies performed in colonic homogenates include determination of myeloperoxidase (MPO) activity,glutathione (GSH) content, leukotriene B4 (LTB4) and tumor necrosis factor α (TNF-α) levels, as well as inducible nitric oxide synthase (iNOS) expression. In addition, the luminal contents obtained from colonic samples were used for microbiological studies, in order to determine Lactobacilli and Bifidobacteria counts.RESULTS: Treatment of colitic rats with L. salivarius ssp.salivarius resulted in amelioration of the inflammatory response in colitic rats, when compared with the corresponding control group without probiotic treatment. This antiinflammatory effect was evidenced macroscopically by a significant reduction in the extent of colonic necrosis and/or inflammation induced by the administration of TNBS/ethanol (2.3+0.4 cm vs3.4+0.3 cm in control group,P<0.01) and histologically by improvement of the colonic architecture associated with a reduction in the neutrophil infiltrate in comparison with non-treated colitic rats. The latter was confirmed biochemically by a significant reduction of colonic MPO activity (105.3+26.0 U/g vs180.6±21.9 U/g, P<0.05), a marker of neutrophil infiltration. The beneficial effect was associated with an increase of the colonic GSH content (1 252±42 nmol/g vs 1 087+51 nmol/g, P<0.05),which is depleted in colitic rats, as a consequence of the oxidative stress induced by the inflammatory process. In addition, the treatment of colitic rats with L. salivarius resulted in a significant reduction of colonic TNF-α levels (509.4±68.2 pg/g vs782.9±60.1 pglg, P<0.01) and in a lower colonic iNOS expression, when compared to TNBS control animals without probiotic administration. Finally,treated colitic rats showed higher counts of Lactobacilli species in colonic contents than control colitic rats, whereas no differences were observed in Bifidobacteria counts.CONCLUSION: Administration of the probiotic L. salivarius ssp. salivarius CECT5713 facilitates the recovery of the inflamed tissue in the TNBS model of rat colitis, an effect associated with amelioration of the production of some of the mediators involved in the inflammatory response in the intestine, such as cytokines, including TNF-α and NO. This beneficial effect could be ascribed to its effect on the altered immune response that occurs in this inflammatory condition. | Laura Peran Desiree Camuesco Monica Comalada Ana Nieto Angel Concha Maria Paz Diaz-Ropero Monica Olivares Jordi Xaus Antonio Zarzuelo Julio Galvez | 2005 | World Journal of Gastroenterology2005，11，33： | 17 |
| 2 | Decorin inhibits macrophage colony-stimulating factor proliferation of macrophages and enhances cell survival through induction of p27 (Kipl) and p21 (Waf1)显示文摘 | Xaus J Comalada M Card ó M | | 0，，07： | 1 |
| 3 | In vivo quer- citrin anti-inflammatory effect involves release of querce- tin, which inhibits inflammation through down-regulation of the NF-~B pathway 显示文摘 | Comalada M Camuesco D Sierra S | 2005 | Eur J Immunol2005，35，2： | 1 |
| 4 | In vivo quercitrin anti inflammatory effect involves release of quercctin,which inhibits inflammation through down-regulation of the NF-kappaB pathway显示文摘 | Comalada M Camuesco D Sierra S | | 0，，02： | 1 |
| 5 | MKP-1 : a critical phosphatase in the biology of macrophages controlling the switch between proliferation and activation 显示文摘 | Comalada M Lloberas J Celada A | 2012 | Eur J Immunol2012，42，8： | 1 |
| 6 | In vivo quercitrin an- ti-inflammatory effect involves release of quercetin, which inhibits inflammation through down-regulation of the NF-KB pathway显示文摘 | Comalada M Camuesco D Sierra S | 2005 | Eur J Immunol2005，35，2： | 1 |
| 7 | Preventativeeffects of lactulose in the trinitrobenzenesulphonic acid model of ratcolitis显示文摘 | CAMUESCO D PERAN L COMALADA M | 2005 | Inflammatory Bowel Diseases2005，11，3： | 1 |
| 8 | Intestinal anti-inflammatory activity of combined quercitrin and dietary dive oil supplemented with fish oil,rich in EPA and DHA(N-3) polyunsaturated fatty acids,in rats with DSS-induced colitis显示文摘 | Camuesco D Comalada M Concha A | 2006 | Clin Nutr2006，25，3： | 1 |
| 9 | Preventative effects of lactulose in the trinitrobenzenesulphonic acid model of rat colitis 显示文摘 | Camuesco D Peran L Comalada M | 2005 | Inflammatory Bowel Diseases2005，11，3： | 1 |
| 10 | Granulocyte-macrophage colony-stimulating factor increases L-arginine transport through the induction of CAT2 in bone marrow-derived macrophages显示文摘 | Martin L Comalada M Marti L | 2006 | Am J Physiol Cell Physiol2006，290，： | 1 |
| 11 | Cyclophilin A is required for M-CSF-dependent macrophage proliferation显示文摘 | Sanchez-Tillo E Wojciechowska M Comalada M | 2006 | Eur J Immunol2006，36，9： | 1 |
| 12 | A comparative study of the pre- ventative effects exerted by three probiotics,Bifidobacterium lactis, LactobaciUus casei and Lactobacillus acidophilus,in the TNBS model of rat colitis显示文摘 | Peran L Camuesco D Comalada M | 2007 | J Appl Microbiol2007，103，4： | 1 |
| 13 | The combination of fructooligosaccharides and resistant starch shows prebiotic additive effects in rats显示文摘 | Maria Elena Rodríguez-Cabezas Desirée Camuesco Belén Arribas Natividad Garrido-Mesa Mònica Comalada Elvira Bailón Margarita Cueto-Sola Pilar Utrilla Eduardo Guerra-Hernández Carlos Pérez-Roca Julio Gálvez Antonio Zarzuelo | 2010 | Clinical Nutrition2010，，6： | 1 |
| 14 | Preventativeeffects of lactulose in the trinitrobenzenesulphonic acid model of ratcolitis显示文摘 | CAMUESCO D PERAN L COMALADA M | 2005 | Inflamm Bowel Dis2005，11，3： | 1 |
| 15 | In vivo quercitrin anti-inflammatory effect involves release of quercetin, which inhibits inflammation through down-regulation of the NF-kappa B pathway显示文摘 | Comalada M Camuesco D Sierra S | 2005 | Eur J Immunol2005，35，58： | 1 |
| 16 | Inhibition of pro - inflammatory markers in primary bone marrow - derived mouse macrophages by naturally occurring fla- vonoids:Analysis of the structure - activity relationship 显示文摘 | Comalada M Ballester I Bailon E | 2006 | Biochemical Pharmacology2006，72，8： | 1 |
| 17 | Macrophage col- ony - stimulating factor - dependent macrophage proliferation is medi- ated through a calcineurin - independent but immunophilin - depend- ent mechanism that mediates the activation of external regulated kina- ses显示文摘 | Comalada M Valledor AF Sanchez - Tillo E | 2003 | Eur J Immunol2003，33，11： | 1 |
| 18 | In vivo quercitrin anti-inf lammatory effect involves release of quercetin,which inhibits inflammation through down-regulat ion of the NF-kappa B pathway显示文摘 | Comalada M Camuesco D Sierra S | 2005 | Eur J Immunol2005，35，2： | 1 |
| 19 | Decorin inhibits macrophage colony-stimulating factor proliferation of macrophages and enhances cell survival through nduction of p27Kip1 and p21 Waf显示文摘 | Jordi X Comalada M | 2001 | BLOOD2001，98，7： | 1 |
| 20 | Lactobacillus fermentum,a probiotic capable to release glutathione,prevents colonic inflammation in the TNBS model of rat colitis 显示文摘 | PERAN L CAMUESCO D COMALADA M | 2005 | Int J Colorectal Dis2005，29，： | 1 |
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