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| 1 | Construction and selection of the natural immune Fab antibody phage display library from patients with colorectal cancer显示文摘AIM: To construct the natural immune Fab antibody phage display libraries of colorectal cancer and to select antibodies related with colorectal cancer.METHODS: Extract total RNA from tissue of local cancer metastasis lymph nodes of patients with colorectal cancer.RT-PCR was used to amplify the heavy chain Fd and light chain к and the amplification products were inserted successively into the vector pComb3 to construct the human libraries of Fab antibodies. They were then panned by phage display technology. By means of Dot immunoblotting and ELISA, the libraries were identified and the Fab phage antibodies binding with antigens of colorectal cancer were selected.RESULTS: The amplified fragments of Fd and к gained by RT-PCR were about 650bp. Fd and к PCR products were subsequently inserted into the vector pComb3, resulting in a recombination rate of 40% and the volume of Fab phage display library reached 1.48 x 106. The libraries were enriched about 120-fold by 3 cycles of adsorption-elution- multiplication (panning). Dot immunoblotting showed Fab expressions on the phage libraries and ELISA showed 5clones of Fab phage antibodies which had binding activities with antigens of colorectal cancer.CONCLUSION: The natural immune Fab antibody phage display libraries of colorectal cancer were constructed. They could be used to select the relative antibodies of colorectal cancer. | Bao-Ping Wu~1 Bing Xiao~1 Tian-Mo Wan~1 Ya-Li Zhang~1 Zhen-Shu Zhang~1 Dian-Yuan Zhou~1 Zhuo-Sheng Lai~1 Chun-Fang Gao~2 1 Institute for Digestive Diseases,Nanfang Hospital,Guangzhou 510515,Guangdong Province,China2 Surgical Department of Colon and Rectum,150 Central Hospital,Luoyang 471031,Henan Province,China | 2001 | World Journal of Gastroenterology2001，7，6： | 9 |
| 2 | Redefining Budd-Chiari syndrome:A systematic review显示文摘AIM:To re-examine whether hepatic vein thrombosis(HVT)(classical Budd-Chiari syndrome)and hepatic vena cava-Budd Chiari syndrome(HVC-BCS)are the same disorder.METHODS:A systematic review of observational studies conducted in adult subjects with primary BCS,hepatic vein outflow tract obstruction,membranous obstruction of the inferior vena cava(IVC),obliterative hepatocavopathy,or HVT during the period of January2000 until February 2015 was conducted using the following databases:Cochrane Library,CINAHL,MEDLINE,Pub Med and Scopus.RESULTS:Of 1299 articles identified,26 were included in this study.Classical BCS is more common in women with a pure hepatic vein obstruction(49%-74%).HVCBCS is more common in men with the obstruction often located in both the inferior vena cava and hepatic veins(14%-84%).Classical BCS presents with acute abdominal pain,ascites,and hepatomegaly.HVC-BCS presents with chronic abdominal pain and abdominalwall varices.Myeloproliferative neoplasms(MPN)are the most common etiology of classical BCS(16%-62%)with the JAK2V617-F mutation found in 26%-52%.In HVCBCS,MPN are found in 4%-5%,and the JAK2V617-F mutation in 2%-5%.Classical BCS responds well to medical management alone and 1st line management of HVC-BCS involves percutaneous recanalization,with few managed with medical management alone.CONCLUSION:Systematic review of recent data suggests that classical BCS and HVC-BCS may be two clinically different disorders that involve the disruption of hepatic venous outflow. | Naomi Shin Young H Kim Hao Xu Hai-Bin Shi Qing-Qiao Zhang Jean Paul Colon Pons Ducksoo Kim Yi Xu Fei-Yun Wu Samuel Han Byung-Boong Lee Lin-Sun Li | 2016 | World Journal of Hepatology2016，8，16： | 6 |
| 3 | Insights into the role of jasmonic acid-mediated defenses against necrotrophic and biotrophic fungal pathogens显示文摘Jasmonic 酸(JA ) 是涉及开发,对伤害的回答和病原体攻击的一个自然荷尔蒙管理者。在病原体的感觉之上, JA 被综合并且调停开始各种各样的防卫回答的发信号的串联。传统地, necrotrophic 真菌被显示了是通过 JA 受体的 JAdependent 防卫的主要使活跃之物, COI1。相反地,感染 biotrophic 真菌的植物古典主义地与压制调停 JA 的回答被联系了。然而,最近的证据显示出那某些 biotrophic 真菌的种类也触发调停 JA 的回答的激活,在 JA 发信号缺乏的异种在危险性显示出增加到某些 biotrophic 真菌的病原体。这些调查结果对真菌的 biotrophs 在防卫为 JA 建议一个新角色。这评论将集中于推进我们对 biotrophic 和 necrotrophic 真菌涉及防卫的 JA 家属回答的知识的最近的研究。 | Christopher J. ANTICO Chad COLON Taylor BANKS Katrina M. RAMONELL | 2012 | Frontiers in Biology2012，7，1： | 5 |
| 4 | 无线局域网802.11a标准展望显示文摘 | Sean Colon | 2002 | 信息技术与标准化2002，，1： | 2 |
| 5 | 显示文摘 | Colon G Hidalgo M C Navio J A | 2002 | Appl Cata A2002，231，： | 2 |
| 6 | Survival after laparoscopic surgery versus open surgery for colon cancer: long-term outcome of a randomised clinical trial 显示文摘 | Colon Cancer Laparoscopic or Open Resection Study Group Buunen M Veldkamp R | 2009 | Lancet Oncoi2009，10，1： | 1 |
| 7 | Requirements for the processing of antigens by antigen -presenting B cells显示文摘 | Chestnut R Colon S Grey H | 1982 | J Immunol1982，129，： | 1 |
| 8 | Mammoscopy: the endoscopic intracapsular evaluation of mammary prosthesis显示文摘 | | 1993 | Plast Reconstr Surg1993，91，2： | 1 |
| 9 | Membranoproliferative glomerulonephritis and Mycoplasma pneumoniae infection 显示文摘 | Cochat P Colon S Bosshard S | 1985 | Arch Fr Pediatr1985，42，1： | 1 |
| 10 | Photocatalytic deactivation of commercial TiO2 samples during simultaneous photoreduction of Cr(Ⅵ) and photooxidation of salicylic acid显示文摘 | Colon G Hidalgo M C Navio J A | 2001 | Journal of Photochemistry and Photobiology A:Chemistry2001，138，1： | 1 |
| 11 | Influence of carboxylic acid on the photocatalytic reduction of Cr(Ⅵ) using commercial TiO2显示文摘 | Colon G Hidalgo M C Navio J A | | 0，，： | 1 |
| 12 | Visible-light driven TiO2 photocatalysis from Ti-oxychloride precursors 显示文摘 | Sampedro P Colon G Fernandez-garci M | 2008 | Chemistry2008，199，23： | 1 |
| 13 | Mammoscopy and endoscopic implant and breast tissue evaluation显示文摘 | | 1995 | Clin Plast Surg1995，22，4： | 1 |
| 14 | Osteopontin dysregulationand lytic bone lesions in multiple myeloma 显示文摘 | Robbiani DF Colon K Ely S | 2007 | Hematol Onco2007，25，1： | 1 |
| 15 | Edema from intracerebral hemorrhage:the role of thrombin显示文摘 | Lee KR Colon GP Betz AL | 1996 | J Neurosurg1996，84，： | 1 |
| 16 | Effects of radio-therapy and of differences in the extent of surgery for earlbreast cancer on local recurrence and 15-year survival: anoverview of the randomised trials 显示文摘 | CLARKE M COLONS R DARBY S | 2005 | Lancet2005，366，9503： | 1 |
| 17 | A study of 10 medical centres of the safety and efficacy of 48 flexible sigmoidoscopies and 8 colonoscupies during pregnancy with follow-up of fetal outcome and with comparison to control groups显示文摘 | Cappell M S Colon V J Sidhom O A | 1996 | Dig Dis Sci1996，41，12： | 1 |
| 18 | Advancing insulin thera-py in type 2 diabetes previously treated with glargine plus oral agents :prandial premixed ( insulin lispro protamine suspension/li?pro) versusbasal/bolus ( glargine/lispro) therapy 显示文摘 | Rosenstock J Ahmann AJ Colon O | 2008 | Diabetes Care2008，31，1： | 1 |
| 19 | Survival after laparoscopic surgery versus open surgery for colon cancer: long- term outcome of a randomised clinical trial 显示文摘 | Colon Cancer Laparoscopic or Open Resection Study Group Buunen M Veldkamp R Hop WC | 2009 | Lancet Oncol2009，10，： | 1 |
| 20 | Edema from intraeerebralhemorrhage:the role of thrombin显示文摘 | Colon GP Betz AL | 1996 | J Neurosnrg1996，84，： | 1 |
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