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| 1 | The Role of MedicationNoncompliance and Adverse Drug Reactions in Hospitalizations of the Elderly显示文摘 | Col N Fanale J E Kronholm P | 1990 | Arch Intern Med1990，150，4： | 1 |
| 2 | Condensation in a square minichannel: application of the VOF method 显示文摘 | Bortolin S Da Riva E Del Col D | 2014 | Heat Transfer Engineering2014，35，2： | 1 |
| 3 | Seven-year experience with chordal replacement with expanded poly-tetrafluoroethylene in floppy mitral valve 显示文摘 | Zussa C Polesel E Col UD | 1994 | J Thorac Cardiovase Surg1994，108，1： | 1 |
| 4 | Microalbuminuria in systolic and diastolic chronic heart failure patients显示文摘 | Orea-Tejeda A Colín-Ramírez E Hernández-Gilsoul T | 2008 | Cardiol J2008，15，： | 1 |
| 5 | Contribution of polymorphisms in the apolipoprotein AI-CⅢ-AIV cluster to hyperlipidaemia in patients with gout显示文摘 | Cardona F Tinahones FJ Col antes E | | 0，，： | 1 |
| 6 | Computer-assisted osteotomies for genu varum deformity:which osteotomy for which varus显示文摘 | Saragaglia D Mercier N Col e PE | | 0，，02： | 1 |
| 7 | Waist circumference and fat intake are associated with high blood pressure in Mexican children aged 8 to 10 years显示文摘 | Col(i)n-Ram(i)rez E Castillo-Mart(i)nez L Orea-Tejeda A | 2009 | J Am Diet Assoc2009，109，6： | 1 |
| 8 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 9 | The significance of microsaccades for vision and oculomotor control显示文摘 | Col ewijn H Kowler E | | 0，，14： | 1 |
| 10 | Effect of gravity during conden- sation of R134a in a circular minichannel VOF simulation of an- nular condensation 显示文摘 | RIVA E D COL D D | 2011 | Microgravity Science and Technology2011，，23： | 1 |
| 11 | The importance of turbulence during condensation in a horizontal cir- cular minichannel 显示文摘 | RIVA E D COL D D GARIMELLA S V | 2012 | International Journal of Heat and Mass Transfer2012，55，1314： | 1 |
| 12 | Condensation ina square minichannel: Application of the VOF method显示文摘 | BORTOLIN S RIVA E D COL 13 D | 2014 | Heat Transfer Engineering2014，，2： | 1 |
| 13 | Numerical simulation of laminar liquid film condensation in a horizontal circular minichannel 显示文摘 | RIVA E D COL D D | 2012 | Journal of Heat Transfer -Transactions of the Asme2012，，05： | 1 |
| 14 | Effect of gravity during conden- sation of R134a in a circular minichannel显示文摘 | RIVA E D COL D D | 2011 | Microgravity Sci- ence and Technology2011，，1： | 1 |
| 15 | Performance of a large capacity propane heat pump with low charge heat ex- changers 显示文摘 | Cavallini A Riva E D Col D D | 2010 | International Journal of Refrigeration2010，33，2： | 1 |
| 16 | Local recurrence and survival rates after extended systematic lymph-node dissection for surgical treatment of rectal cancer显示文摘 | Hasdemir O Col C Yalqin E | 2005 | Hepatogastroenterulogy2005，52，62： | 1 |
| 17 | The viral control of cellular acetylation signaling显示文摘 | Caron C Col E Khochbin S | 2003 | Bioessays2003，25，1： | 1 |
| 18 | Local recurrence and survival rates after extended systematic lymph-node dissection for surgical treatment of rectal cancer 显示文摘 | Hasdemir O Col C Yalcin E | 2005 | Hepatogastroenterology2005，52，62： | 1 |
| 19 | Incidence and reasons for reoperation after minimal y invasive unicompartmental knee arthroplasty显示文摘 | Hamilton WG Col ier MB Tarabee E | | 0，，2： | 1 |
| 20 | Performance of a largecapacity propane heat pump with low charge heat exchang-ers 显示文摘 | Cavallini A Da Riva E Del Col D | 2010 | International Journal of Refrigeration2010，33，2： | 1 |
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