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| 1 | Black soldier fly and gut health in broiler chickens:insights into the relationship between cecal microbiota and intestinal mucin composition显示文摘Background:The relationship between diet and intestinal microbiota and mucin composition appears to be fundamental for poultry gut health.The effects of insect meal(whose role as alternative feed ingredient is now well recognized)on gut microbiota and mucin composition have recently been reported in Tenebrio molitor-fed free-range and broiler chickens,but no data are currently available for Hermetia illucens(HI)-fed broilers.The present study evaluated the effects of dietary HI meal inclusion on cecal microbiota and intestinal mucin composition of broiler chickens.Results:A total of 256 male broiler chickens were allotted to 4 dietary treatments(control diet[C]and 5%,10%and 15%HI meal inclusion,with 8 replicate pens/treatment and 8 birds/pen)and slaughtered at 35 d of age(2 animals/pen,16 birds/diet).The cecal microbiota assessment by 16S rRNA amplicon based sequencing showed lower alpha diversity in HI15 chickens(Shannon,P<0.05)and higher beta diversity(Adonis and ANOSIM,P<0.001)in birds fed HI diets than C.Furthermore,HI15 birds displayed significant increase of the relative abundance of Proteobacteria phylum(False Discovery Rate[FDR]<0.05)when compared to HI10.L-Ruminococcus(Ruminococcus from Lachnospiraceae family),Faecalibacterium,Blautia and Clostridium genera were found to be characteristic of HI5 cecal microbiota(FDR<0.05),while broiler chickens fed HI10 and HI15 diets were characterized(FDR<0.05)by Lactobacillus and Ruminococcus(HI10)and Bacteroides,Roseburia and Helicobacter genera(HI15).Periodic-acid Schiff,Alcian Blue pH 2.5 and high iron diamine staining on small and large intestine also demonstrated lower mucin staining intensity in the intestinal villi of HI10 and HI15 birds than C(P<0.05).Conclusions:Dietary HI meal utilization at low inclusion levels(i.e.,5%)positively influenced either the cecal microbiota or the gut mucin dynamics in terms of selection of potentially beneficial bacteria and increase in villi mucins.However,high inclusion levels(in particular the 15%)may have a negative influence in terms of partial reduction of microbial complexity,reduction of potentially beneficial bacteria,selection of bacteria with mucolytic activity and decrease in villi mucins. | Ilaria Biasato Ilario Ferrocino Sihem Dabbou Rocchina Evangelista Francesco Gai Laura Gasco Luca Cocolin Maria Teresa Capucchio Achille Schiavone | 2020 | Journal of Animal Science and Biotechnology2020，11，3： | 4 |
| 2 | The rpoB gene as a target for PCR-DGGE analysis to follow lactic acid bacterial population dynamics during food fermentations显示文摘 | RANTSIOU K COMI G COCOLIN L | 2004 | Food Microbiology2004，21，4： | 1 |
| 3 | Direct profiling of the yeast dynamics in wine fermentations 显示文摘 | Cocolin L Bisson L F Mills D A | 2000 | FEMS Microbi- ology Letters2000，189，1： | 1 |
| 4 | Ecology of lactic acid bacteria in Italian fermented sausages:Isolation,identification and molecular characterization显示文摘 | URSO R COMI G COCOLIN L | 2006 | Systematic and Applied microbiology2006，29，8： | 1 |
| 5 | Lac-tic acid bacteria ecology of three traditional fermented sau-sages produced in the North of Italy as determined by mo-lecular methods显示文摘 | Luca Cocolin Paola Dolci Kalliopi Rantsiou | 2009 | Meat Science2009，82，1： | 1 |
| 6 | Evaluation of PCR primers for denaturing gradient gel electrophoresis analysis of fungal communities in compost 显示文摘 | Marshall M N Cocolin L Mills D A | 2003 | Journal of Applied Microbiology2003，95，： | 1 |
| 7 | A nested PCR method to detect Listeria momocytogenes in artificially contaminated blood specimens显示文摘 | Cocolin L Manzano M Cantomi C | 1997 | Res Microbiol1997，148，： | 1 |
| 8 | An application of PCR- DGGE analysis to profile the yeast populations in raw milk 显示文摘 | Cocolin L Aggio D Manzano M | 2002 | International Dairy Journal2002，12，5： | 1 |
| 9 | An application of PCR-DGGE Analysis to profile the yeast populations in raw milk显示文摘 | Cocolin L Aggio D Manzano M | 2002 | a2002，12，： | 1 |
| 10 | Studyof the ecology of fresh sausages and characterization of populations oflactic acid bacteria by molecular methods 显示文摘 | Cocolin L Rantsiou K lacumin L Urso R Cantoni C Comi G | 2004 | Appl Environ Microbiol2004，70，4： | 1 |
| 11 | Development of a PCR microplate-capture hybridization method for simple,fast and sensitive detection of Salmonella serovars in food显示文摘 | Manzano M Cocolin L Astori G | 1998 | Molecular and Cellular Probes1998，12，： | 1 |
| 12 | Temperature gradient gel electrophoresis of the amplified product of a small 16S rRNA gene fragment for the identification of Listeria species isolated from food 显示文摘 | MANZANO MARISA COCOLIN LUCA CANTONI CARLO | 2000 | J Food Protect2000，63，5： | 1 |
| 13 | Denaturing gradient gel electrophoresis analysis of the 16S rRNA gene V1 region to monitor dynamic changes in the bacterial population during fermentation of Italian sausages显示文摘 | COCOLIN L MANZANO M CANTONI C | 2001 | Applied and Environmental Microbiology2001，67，11： | 1 |
| 14 | Denaturing Gradient Gel Electrophoresis Analysis of the 16S rRNA Gene V1 Region To Monitor Dynamic Changes in the Bacterial Population during Fermentation of Italian Sausages 显示文摘 | Cocolin L Manzano M Cantoni C | 2001 | Appl Environ Microbiol2001，67，11： | 1 |
| 15 | Analysis of PCR-based methods for characterization of Listeria monocytogenesstrains isolated from different sources显示文摘 | COCOLIN L STELLA S NAPPI R | 2005 | Int J Food Microbiol2005，1，： | 1 |
| 16 | Yeast diversity and persistence in Botrytis-affected wine fermentations 显示文摘 | Mills D A Johannsen E A Cocolin L | 2002 | Appl Environ Microbiol2002，68，10： | 1 |
| 17 | Denaturing gradient gel electrophoresis analysis of the 16s rDNA geneV1 region to monitor dynamic changes in the bacterial population during fermentation of Italian sausage显示文摘 | Cocolin L Manzano M Cantoni C | 2001 | Appl Environ Microbiol2001，67，11： | 1 |
| 18 | Use of Polymerase Chain Reaction and Restriction Enzyme Analysis to Direcdy Detect and Identify Salmonella Typhimurium in Food 显示文摘 | COCOLIN L MANZANO M CANTOH C | 1998 | Journal Applied Microbiology1998，85，4： | 1 |
| 19 | The late blowing in cheese: a new molecular approach based on PCR and DGGE to study the microfloral ecology of the alteration process显示文摘 | COCOLIN L INNOCENTE N BIASUTTI M | 2004 | International Journal of Food Microbiology2004，90，： | 1 |
| 20 | The rpoB gent as a target for PCR-DGGE analysis to follow lactic acid bacterial population dynamics during food fermentations显示文摘 | RANTSIOU K COMI G COCOLIN L | 2004 | Food Microbiology2004，21，4： | 1 |
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