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| 1 | Normal and disease-related biological functions of Twist1 and underlying molecular mechanisms显示文摘这篇文章在开发,基因疾病和癌症考察分子的结构,表达式模式,生理的函数,病理学的角色和 Twist1 的分子的机制。Twist1 是基本 helix-loop-helix 包含域的抄写因素。以便绑 Nde1 电子盒子元素并且激活或镇压形成 homo-dimers 或 hetero-dimers 它的目标基因。在开发期间, Twist1 为中层说明和区别是必要的。人的 Twist1 基因的异质接合的 loss-of-function 变化包括 Saethre-Chotzen 症候群引起几疾病。Twist1 空的老鼠胚胎与打开的头部的神经试管和有缺点的头间充组织死,体节和手足芽。Twist1 在胸被表示,肝,前列腺,胃并且癌症的另外的类型,和它的表示通常与侵略、变形的癌症显型被联系。在癌症房间, Twist1 是由包括 SRC-1 的多重因素的 upregulated, STAT3, MSX2, HIF-1α,连接 integrin 的 kinase 和 NF-κ B。Twist1 显著地提高上皮间充质的转变(EMT ) 和癌症房间移植和侵略,因此支持癌症转移。Twist1 由直接镇压部分地支持 EMT 由招募的 E-cadherin 表示改变的 nucleosome 和为基因压抑并且由 upregulating Bmi1 的 deacetylase 建筑群, AKT2, YB-1,等等。新兴的证据也建议 Twist1 在扩大和癌症干细胞的化学疗法的抵抗起一个作用。Twist1 由支持 Twist1 功能的调节的人的转移和鉴定的机制的进一步的理解可以为开发新策略禁止 EMT 和癌症转移保持诺言。 | Qian Qin Young Xu Tao He Chunlin Qin Jianming Xu | 2012 | Cell Research2012，22，1： | 66 |
| 2 | Modeling and Simulation on Axial Piston Pump Based on Virtual Prototype Technology显示文摘A particular emphasis is placed on the virtual prototype technology(VPT) of axial piston pump.With this technology it is convenient and flexible to build a complicated 3D virtual based on real physical model.The actual kinematics pairs of the parts were added on the model.The fluid characters were calculated by hydraulic software.The shape of the parts, the flexible body of parts, etc were improved in this prototype.So the virtual prototype of piston pump can work in computer like a real piston pump, and the flow ripple, pressure pulsation, motion principle, stress of parts, etc can be investigated.The development of the VPT is introduced at the beginning, and the modeling process of the virtual prototype is explained.Then a special emphasis is laid on the relationship between the dynamics model and the hydraulic model, and the simulations on the flow ripple, pressure pulsation, motion principle, the stress and strain distribution of the middle shaft and piston are operated.Finally, the advantages and disadvantages of the VPT are discussed.The improved virtual prototype of piston pump more tally with the real situation and the VPT has a great potential in simulation on hydraulic components. | ZHANG Bin XU Bing XIA Chunlin YANG Huayong | 2009 | Chinese Journal of Mechanical Engineering2009，22，1： | 29 |
| 3 | Combining CLUE-S and SWAT Models to Forecast Land Use Change and Non-point Source Pollution Impact at a Watershed Scale in Liaoning Province, China显示文摘Non-point source(NPS) pollution has become a major source of water pollution. A combination of models would provide the necessary direction and approaches designed to control NPS pollution through land use planning. In this study, NPS pollution load was simulated in urban planning, historic trends and ecological protection land use scenarios based on the Conversion of Land Use and its Effect at Small regional extent(CLUE-S) and Soil and Water Assessment Tool(SWAT) models applied to Hunhe-Taizi River Watershed, Liaoning Province, China. Total nitrogen(TN) and total phosphorus(TP) were chosen as NPS pollution indices. The results of models validation showed that CLUE-S and SWAT models were suitable in the study area. NPS pollution mainly came from dry farmland, paddy, rural and urban areas. The spatial distribution of TN and TP exhibited the same trend in 57 sub-catchments. The TN and TP had the highest NPS pollution load in the western and central plains, which concentrated the urban area and farm land. The NPS pollution load would increase in the urban planning and historic trends scenarios, and would be even higher in the urban planning scenario. However, the NPS pollution load decreased in the ecological protection scenario. The differences observed in the three scenarios indicated that land use had a degree of impact on NPS pollution, which showed that scientific and ecologically sound construction could effectively reduce the NPS pollution load in a watershed. This study provides a scientific method for conducting NPS pollution research at the watershed scale, a scientific basis for non-point source pollution control, and a reference for related policy making. | LIU Miao LI Chunlin HU Yuanman SUN Fengyun XU Yanyan CHEN Tan | 2014 | Chinese Geographical Science2014，24，5： | 15 |
| 4 | Enhanced efficiency of cadmium removal by Boehmeria nivea(L.) Gaud. in the presence of exogenous citric and oxalic acids显示文摘Boehmeria nivea(L.)Gaud.is a potential candidate for the remediation of Cd contaminated sites.The present investigation aims to explore Cd tolerance threshold and to quickly identify the role of exogenous organic acids in Cd uptake and abiotic metal stress damage.Elevated Cd levels(0–10 mg/L)resulted in an obvious rise in Cd accumulation,ranging from268.0 to 374.4 in root and 25.2 to 41.2 mg/kg dry weight in shoot,respectively.Citric acid at1.5 mmol/L significantly facilitated Cd uptake by 26.7%in root and by 1-fold in shoot,respectively.Cd translocation efficiency from root to shoot was improved by a maximum of66.4%under 3 mmol/L of oxalic acid.Citric acid exhibited more prominent mitigating effect than oxalic acid due to its stronger ligand affinity for chelating with metal and avoiding the toxicity injury of free Cd ions more efficiently.The present work provides a potential strategy for efficient Cd remediation with B.nivea. | Huaying Li Yunguo Liu Guangming Zeng Lu Zhou Xin Wang Yaqin Wang Chunlin Wang Xinjiang Hu Weihua Xu | 2014 | Journal of Environmental Sciences2014，26，12： | 10 |
| 5 | Comparable Characterization of Nanocellulose Extracted from BleachedSoftwood and Hardwood Pulps显示文摘In this study, the characteristics of nanocellulose extracted from bleached softwood and hardwood pulps by formic acid hydrolysis followed by TEMPO-mediated oxidation were compared using transmission electron microscopy(TEM), atomic force microscopy(AFM), Fourier transform infrared analysis(FT-IR), X-ray diffraction(XRD), and thermal gravimetric analysis(TGA). The experimental results showed that the nanocellulose products derived from spruce pulp exhibited a relatively larger particle size, higher crystallinity, and higher thermal stability, compared with the corresponding products obtained from aspen pulp under the same conditions.Furthermore, the study helped establish that the properties of the nanocellulose products were highly dependent on the nature of the starting materials under identical processing conditions. | Bin Li WenYang Xu Dennis Kronlund Jan-Erik Eriksson Anni Maattanen Stefan Willfor ChunLin Xu | 2018 | Paper And Biomaterials2018，3，4： | 7 |
| 6 | microRNA-218 suppresses the proliferation, invasion and promotes apoptosis of pancreatic cancer cells by targeting HMGB1显示文摘Objective: To detect the expression profiles of micro RNA-218(mi R-218) in human pancreatic cancer tissue(PCT) and cells and their effects on the biological features of human pancreatic cancer cell line PANC-1 and observe the effect of mi R-218 on the expression of the target gene high mobility group box 1(HMGB1), with an attempt to provide new treatment methods and strategies for pancreatic cancer.Methods: The expressions of mi R-218 in PCT and normal pancreas tissue as well as in various pancreatic cancer cell lines including As PC-1, Bx PC-3, and PANC-1 were determined with quantitative real-time reverse transcription polymerase chain reaction(q RT-PCR). The change of mi R-218 expression in PANC-1 cells was detected using qR T-PCT after the transfection of miR-218 mimic for 48 h. Cell Counting Kit-8(CCK-8) was applied for detecting the effect of mi R-218 on the activity of PANC-1 cells. The effects of mi R-218 on the proliferation and apoptosis of PANC-1 cells were analyzed using the flow cytometry. The effect of mi R-218 on the migration of PANC-1 cells was detected using the Trans-well migration assay. The HMGB1 was found to be a target gene of mi R-218 by luciferase reporter assay, and the effect of mi R-218 on the expression of HMGB1 protein in cells were determined using Western blotting.Results: As shown by q RT-PCR, the expressions of mi R-218 in PCT and in pancreatic cancer cell line significantly decreased when compared with the normal pancreatic tissue(NPT)(P<0.01). Compared with the control group, the miR-218 expression significantly increased in the PANC-1 group after the transfection of mi R-218 mimic for 48 h(P<0.01). Growth curve showed that the cell viability significantly dropped after the overexpression of mi R-218 in the PANC-1 cells for two days(P<0.05). Flow cytometry showed that the S-phase fraction significantly dropped after the overexpression of mi R-218(P<0.01) and the percentage of apoptotic cells significantly increased(P<0.01). As shown by the Trans-well migration assay, the enhanced mi R-218 expression was associated with a significantly lower number of cells that passed through a Transwell chamber(P<0.01). Luciferase reporter assay showed that, compared with the control group, the relative luciferase activity significantly decreased in the mi R-218 mimic group(P<0.01). As shown by the Western blotting, compared with the control group, the HMGB1 protein expression significantly decreased in the PANC-1 group after the transfection of mi R-218 mimic for 48 h(P<0.01).Conclusions: The mi R-218 expression decreases in human PCT and cell lines. mi R-218 can negatively regulate the HMGB1 protein expression and inhibit the proliferation and invasion of pancreatic cancer cells. A treatment strategy by enhancing the mi R-218 expression may benefit the patients with pancreatic cancer. | Zhe Liu Yuanhong Xu Jin Long Kejian Guo Chunlin Ge Ruixia Du | 2015 | Chinese Journal of Cancer Research2015，27，3： | 7 |
| 7 | Early hyperbaric oxygen therapy inhibits aquaporin 4 and adrenocorticotropic hormone expression in the pituitary gland of rabbits with blast-induced craniocerebral injury显示文摘In the present study, rabbits were treated with hyperbaric oxygen for 1 hour after detonator-blast-induced craniocerebral injury. Immunohistochemistry showed significantly reduced aquaporin 4 expression and adrenocorticotropic hormone expression in the pituitary gland of rabbits with craniocerebral injury. Aquaporin 4 expression was positively correlated with adrenocorticotropic hormone expression. These findings indicate that early hyperbaric oxygen therapy may suppress adrenocorticotropic hormone secretion by inhibiting aquaporin 4 expression. | Jian Huo Jiachuan Liu Jinbiao Wang Yongming Zhang Chunlin Wang Yanyan Yang WenjiangSun Shaonian Xu | 2012 | Neural Regeneration Research2012，7，22： | 6 |
| 8 | Listeria monocytogenes Mutants Carrying Newcastle Disease Virus F Gene Fused to its actA and plcB:In vitro Expression and Immunogenicity in Chickens显示文摘Recombinant Listeria monocytogenes 异种带纽卡斯尔疾病病毒(NDV ) 熔化蛋白质基因 F 被相应 recombination.NDV F 构造或它的截断的碎片 Fa 被用作他们的信号序列下游地集成于行为 A 或 plcB 的模型 heterologous 基因。插入的基因的正确取向被 F 的聚合酶链反应扩大验证或 Fa.The 插入了 F, Fa 在由钠 dodecylsulfate-polyacrylamide 凝胶电泳(SDS 页) 和蛋白质印迹出现的二 recombinantsLm 三角洲 actA-F 和 Lm 三角洲 plcB-Fa 被表示。展出的两 recombinants 作为与父母相比由大约 1.5-2.5 木头把毒力归结为受胎的鸡蛋和老鼠野紧张 10403S .Theywere 另外不比紧张 10403S (P<0.05 ) 侵略。因为对相应病毒紧张或 recombinant 的抗体乳头 ers 截断了熔化蛋白质,口头上地或 intraperitoneally 收到 recombinant 紧张的鸡部分由于非特定的免疫的改进可能被保护免受剧毒的 NDV 挑战的伤害是边缘的。进一步的研究在另外的动物模型被需要看低抗体反应是否由于 L.monocytogenes 或它的 recombinants 的失败源于 theheterologous 基因的不够的表示在鸡坚持或复制。 | Lingli JIANG Chunlin KE Jingjing XU Jianshun CHEN Xueyan CHEN Ning CHEN Jiangbing SHUAI and Weihuan FANG Institute of Preventive Veterinary Medicine,and Zhejiang Provincial Key Laboratory for Preventve Veterinary Medicine,Zhejiang University,Hangzhou 310029,China | 2007 | Acta Biochimica et Biophysica Sinica2007，39，1： | 6 |
| 9 | Optimization of working parameters for puddling and flatting machine in paddy field显示文摘In order to improve the soil preparation quality,a wide puddling and flatting machine for paddy field was designed with the functionality of tillage,soil crushing,puddling,burying stubble and flatting in one-time operation,which can be equipped on tractors with high power.This study focused on the design and analysis of key components of the machine including puddling equipment,flat shovel and balance structure.Optimal results with field trials and response surface analysis showed that the machine can operate optimally when the working speed,puddling depth and angle of flat shovel were 1.05 km/h,16 cm and 31.30°,respectively.The surface flatness standard deviation was 3.7 cm,and slurry degree was 1.03 g/cm3.Field validation test results were consistent with the optimal parameters,which was able to meet agronomic requirements of mechanical planting in China. | Xu Chunlin Zhang Chengliang Li Lianhao Li Mingjin | 2016 | International Journal of Agricultural and Biological Engineering2016，9，3： | 5 |
| 10 | A novel STING agonist-adjuvanted pan-sarbecovirus vaccine elicits potent and durable neutralizing antibody and T cell responses in mice,rabbits and NHPs显示文摘The emergence of SARS-CoV-2 variants and potentially other highly pathogenic sarbecoviruses in the future highlights the need for pan-sarbecovirus vaccines.Here,we discovered a new STING agonist,CF501,and found that CF501-adjuvanted RBD-FC vaccine(CF501/RBD-FC)elicited significantly stronger neutralizing antibody(nAb)and T cell responses than Alum-and cGAMP-adjuvanted RBD-FC in mice.Vaccination of rabbits and rhesus macaques(nonhuman primates,NHPs)with CF501/RBD-FC elicited exceptionally potent nAb responses against SARS-CoV-2 and its nine variants and 41 S-mutants,SARS-CoV and bat SARSr-CoVs.CF501/RBD-FC-immunized hACE2-transgenic mice were almost completely protected against SARS-CoV-2 challenge,even 6 months after the initial immunization.NHPs immunized with a single dose of CF501/RBD-FC produced high titers of nAbs.The immunized macaques also exhibited durable humoral and cellular immune responses and showed remarkably reduced viral load in the upper and lower airways upon SARS-CoV-2 challenge even at 108 days post the final immunization.Thus,CF501/RBD-Fc can be further developed as a novel pan-sarbecovirus vaccine to combat current and future outbreaks of sarbecovirus diseases. | Zezhong Liu Jie Zhou Wei Xu Wei Deng Yanqun Wang Meiyu Wang Qian Wang Ming Hsieh Jingming Dong Xinling Wang Weijin Huang Lixiao Xing Miaoling He Chunlin Tao Youhua Xie Yilong Zhang Youchun Wang Jincun Zhao Zhenghong Yuan Chuan Qin Shibo Jiang Lu Lu | 2022 | Cell Research2022，32，3： | 5 |
| 11 | Gut microbiota contributes to the development of endometrial glands in gilts during the ovary-dependent period显示文摘Background:The hyper-prolificacy Meishan gilts achieved a superior endometrial gland development(EGD)than white crossbred gilts during the ovary-independent period(before 60 d of age).Then,the EGD continues under the management of ovary-derived steroid hormones that regulated by gut microbiota(after 60 d of age).However,whether Meishan gilts’superiority in EGD lasting to the ovary-dependent period(after 60 d of age)and the role of gut microbiota in this period both remain unclear.Methods:Meishan gilts and Landrace x Yorkshire(LxY)gilts were raised under the same housing and feeding conditions until sexual maturity and then we compared their EGD and gut microbiota.Meanwhile,we transplanted fecal microbiota from Meishan gilts to L×Y gilts to explore the role of gut microbiota in EGD.We sampled plasma every 3 weeks and collected the uterus,ovary,liver,and rectal feces after the sacrifice.We then determined the hormone concentrations and expressions of the EGD-related genes.We also profiled the gut microbiota using 16S rDNA sequencing and metabolites of plasma and liver tissue using untargeted metabolomics.Finally,the correlation analysis and significant test was conducted between FMT-shifted gut microbes and EGD-related indices.Results:Meishan gilts have larger endometrial gland area(P<0.001),longer uterine horn length(P<0.01)but lighter uterine horn weight(P<0.05),a distinctive gut microbiota compared with L×Y gilts.Fecal microbiota transplantation(FMT)increased endometrial gland area(P<0.01).FMT markedly shifted the metabolite profiles of both liver and plasma,and these differential metabolites enriched in steroid hormone biosynthesis pathway.FMT increased estradiol and insulin-like growth factor 1 but decreased progesterone dynamically.FMT also increased the expression of the EGD-related genes estrogen receptor 1 gene,epithelial cadherin,and forkhead box protein A2.There is a significant correlation between FMT-shifted gut microbes and EGD-related indices.Conclusion:Sexually matured Meishan gilts achieved a superior EGD than LxY gilts.Meanwhile,gut microbiota contribute to the EGD potentially via regulating of steroid hormones during the ovary-dependent period. | Baoyang Xu Wenxia Qin Yiqin Yan Yimei Tang Shuyi Zhou Juncheng Huang Chunlin Xie Libao Ma Xianghua Yan | 2021 | Journal of Animal Science and Biotechnology2021，12，4： | 4 |
| 12 | Optimized design and experiment of a fully automated potted cotton seedling transplanting mechanism显示文摘In order to improve the accuracy and stability of transplanting machine seedling picking,a seedling pick-up mechanism was designed,which was controlled by a controller and driven by brushless DC servo motor.At the same time,the parameters of the seedling manipulator were optimized:the mathematical model for the seedling pick-up mechanism was established.According to the predetermined trajectory requirements,the objective function and constraint conditions were proposed,and then the optimal size was obtained by a multi-objective genetic algorithm.At last,Automatic Dynamic Analysis of Mechanical Systems(ADAMS)software was used to simulate and analyze the kinematics and trajectory of the seedling pick-up mechanism,and the mechanism was tested to verify the effectiveness of the mechanism prototype.The experiments showed that the success rate of seedling picking was 94.32%,the rate of acceptably planted seedlings was 96.67%,and the rate of excellently planted seedlings was 63.48%. | Xianglei Xue Lianhao Li Chunlin Xu Enquan Li Yujie Wang | 2020 | International Journal of Agricultural and Biological Engineering2020，13，4： | 3 |
| 13 | Synthesis of Amphiphilic Starch Derivatives Using One-pot Synthesis Procedure显示文摘Amphiphilic starch derivatives with high content of functional groups were prepared from potato starch using a one-pot synthesis method with a single reaction medium for the entire procedure. Potato starch was benzylated, followed by the introduction of hydroxypropyltrimethylammonium(HPMA) moieties without the purification of intermediates. The synthesis was performed under heterogeneous conditions, leading to the formation of benzyl 2-hydroxypropyltri methylammonium starch chloride(BnHPMAS) with a total degree of substitution(DS) of up to 1.4. This process improved the efficiency of the preparation of amphiphilic starch derivatives and reduced the time and resources consumed by avoiding a separation process and purification of the intermediate compounds.The DS of BnHPMAS was in the range of 0.36 to 1.4, which could be tuned by varying the molar ratio of the reagents to repeating unit or by changing the reaction temperature, time, and medium. The structure of the amphiphilic starches was characterized using elemental analysis, size exclusion chromatography,fourier transform infrared spectroscopy(FT-IR), and nuclear magnetic resonance(NMR) spectroscopy. Moreover, the surface tension and turbidity of the solutions of the products were measured for their potential application in the removal of dissolved and colloidal substances in paper cycling water. | Tao Song FengXia Yue XueZhu Xu ChunLin Xu Yi-Chen Li FaChuang Lu HaiSong Qi | 2018 | Paper And Biomaterials2018，3，1： | 3 |
| 14 | Potassium sodium niobate based lead-free ceramic for high-frequency ultrasound transducer applications显示文摘The BiAlO_(3)(BA)and(Bi_(0.5)Na_(0.5))ZrO_(3)(BNZ)are selected to form a solid solution with(K_(0.48)Na_(0.52))NbO_(3) via traditional solid state technique to optimize the electrical performance and temperature stability of KNNbased lead-free ceramics,simultaneously.Here we show that doped BA has a great influence on phase structure,morphologies,and electrical properties.The XRD patterns and dielectric constant versus temperature curves reveal that an increase in the BA content results in a transform of phase structures from a coexistence state of rhombohedral,orthorhombic and tetragonal phases to pseudocubic phase.Owing to the construction of R-O-T phase boundary,optimized performances(T_(C)~336℃,d_(33)~306 pC/N,kp=0.48)are obtained in 0.962(K_(0.48)Na_(0.52))NbO_(3)-0.003BiAlO_(3)-0.035(Bi_(0.5)Na_(0.5))ZrO_(3)(KNN-3)ceramics.Based on the sintered KNN-3 ceramic samples,high-frequency ultrasound imaging transducers are designed and fabricated,which exhibits a high center frequency of 24.5 MHz,a broad -6 dB bandwidth of 97% and a high-sensitivity.Finally,the imaging characteristic of the lead-free transducers is demonstrated via ex vivo imaging of biological tissue structure.As environment friendly materials,the excellent electrical and acoustic performance of developed KNN-based ceramics has great potential for practical applications. | Jie Xing Laiming Jiang Chunlin Zhao Zhi Tan Qian Xu Jiagang Wu Qiang Chen Dingquan Xiao Jianguo Zhu | 2020 | Journal of Materiomics2020，6，3： | 3 |
| 15 | Integrated analysis of multi-tissues lipidome and gut microbiome reveals microbiota-induced shifts on lipid metabolism in pigs显示文摘Lipid metabolism is very important for meat quality in pigs.Accumulating evidence shows that gut microbiota can contribute to this physiological process.However,the gut microbiota that function in lipid metabolism and adipogenesis remains unclear.Here,we compared the characteristics of fat deposition and gut microbial community between Laiwu pigs and Duroc(LandraceYorkshire)(DLY)pigs.Fecal microbiota transplantation(FMT)was performed to determine the possible impact of gut microbiota on lipid metabolism in pigs.An integrated analysis of the gut microbiome and lipidome of the small intestine,plasma,and liver was conducted to investigate the effects of FMT on host lipid metabolism.The comparative analysis of the gut microbiome showed higher abundance of Bacteroidetes(P=0.0018)while lower abundance of Firmicutes(P=0.012)in Laiwu pigs,and the microbial composition can be transferred from Laiwu pigs into DLY pigs.Transmission electron microscope and Oil red-O staining were performed to analyze the effects of FMT on lipid deposition in liver,the main target organ for lipid metabolism.The results showed that FMT significantly increased the number of lipid droplets(P=0.0035)and lipid accumulation(P=0.0026)in liver.Furthermore,integrated multi-tissues lipidome analysis demonstrated that the fatty acyls and glycerophospholipids were significantly increased(P<0.01)in intestine and liver,while glycerolipids and fatty acyls were reduced(P<0.01)in plasma.In the small intestine,FMT increased(P<0.01)the relative abundance of polyketides and prenol lipids but reduced(P<0.01)the saccharolipids.Correlation analysis revealed the potential interactions between microbiota and lipid metabolites.Together,our results indicated that the gut microbiota may regulate the lipid metabolism and enhance the accumulation of lipid droplets in the liver of pigs. | Chunlin Xie Xiaoyan Zhu Baoyang Xu Yaorong Niu Xuelei Zhang Libao Ma Xianghua Yan | 2022 | Animal Nutrition2022，，3： | 2 |
| 16 | microRNA-218 promotes gemcitabine sensitivity in human pancreatic cancer cells by regulating HMGB1 expression显示文摘Objective: The purpose of this study was to examine the effect of gemcitabine(GEM) on micro RNA-218(mi R-218) expression in human pancreatic cancer cells.Methods: Quantitative reverse transcription polymerase chain reaction(q RT-PCR) was performed to examine the differences in mi R-218 expression between the GEM-sensitive Bx PC-3 pancreatic cancer cells and GEM-resistant PANC-1 cells. The effect of GEM on the expression of mi R-218 in PANC-1 cells was also investigated. PANC-1 cells were transfected either with HMGB1 si RNA to knock down the expression of HMGB1 or with the recombinant HMGB1 expression vector(pc DNA3.1-HMGB1) to overexpress HMGB1. The effect of ectopic expression of HMGB1 on the apoptosis of mi R-218-transfected and GEMtreated PANC-1 cells was examined by flow cytometric analysis.Results: The mi R-218 expression level was lower in GEM-resistant PANC-1 cells compared to GEMsensitive Bx PC-3 cells(P<0.05). The percentage of apoptotic PANC-1 cells was significantly increased in the mi R-218 mimic + GEM group compared to the mimic ctrl + GEM group and the normal control group(P<0.01). The HMGB1 expression level was markedly decreased in PANC-1 cells transfected with HMGB1 si RNA but was significantly increased in PANC-1 cells transfected with the recombinant HMGB1 expression vector, pc DNA3.1-HMGB1(P<0.01). The proportion of apoptotic PANC-1 cells was significantly lower in the mi R-218 mimic + GEM + pc DNA3.1-HMGB1 group compared to the mi R-218 mimic + GEM + HMGB1 si RNA group(P<0.01).Conclusions: The expression level of mi R-218 was downregulated in the GEM-resistant cell line. mi R-218 promoted the sensitivity of PANC-1 cells to GEM, which was achieved mainly through regulating the expression of HMGB1 in PANC-1 cells. | Zhe Liu Ruixia Du Jin Long Kejian Guo Chunlin Ge Shulong Bi Yuanhong Xu | 2015 | Chinese Journal of Cancer Research2015，27，3： | 2 |
| 17 | Band tillage with fertilizer application for unpuddled transplanting rice in northeast of China显示文摘Many technological innovations have been developed in agriculture for rice cultivation.Farmers still rely on intensive soil tillage,broadcasting fertilizer,excess irrigation water and labor for rice production.Rice cultivation under unpuddled condition in band-tillage(BT)is a new alternative rice cultivation system in the regions where rainfall,fresh water resources,fertilizers and labors are limited.Therefore,the concept of inter-row BT(T-shaped)with row fertilizer application in soils for unpuddled transplanted rice cultivation(T1)was carried out at Fangzheng,Harbin in the northeast of China.The improved system(T1)was compared with traditional practices(T2)in randomized complete block design with three replications.T2 was done with removal of crop residue,full tillage,broadcasting fertilizer and puddled soils by using the conventional machine.Test results showed that all parameters were satisfied and met the requirements of agronomic performances.The variations of fertilizing stability and evenness were 1.84%and 1.72%,respectively.Soil cone penetration resistance was less in T1 than T2 after tillage.Irrigation water was saved by 26.3%in treatment T1 as compared with T2 for wet land preparation,and wet soil resistance depth had no significant difference.Planting depth was significantly different between the treatments which was affected by intensive tillage and puddling in T2 as compared with T1.Soil temperature was nearly similar,but comparatively 0.23°C(on average)higher than traditional system throughout the crop growing season.Crop growth rate and grain yield increased by 8.5%and 5.3%,respectively in band till with fertilizer placed compared to conventional practices of puddled transplanting.The unit production cost(￥/kg of grain yield)was found lower by 12.3%in treatment T1 than T2.The output-input ratio was marginally greater in treatment T1;1.51)as compared to T2;1.32).Short-term findings suggest that inter-row BT system with row fertilizer application could be used in unpuddled rice farming system instead of traditional puddled rice cultivation. | Khokan Kumer Sarker Xu Chunlin Wang Xiaoyan Li Minjin Li Lianhao Liu Guiming | 2016 | International Journal of Agricultural and Biological Engineering2016，9，4： | 2 |
| 18 | Anonymous Authentication without Home Server in Mobile Roaming Networks显示文摘 | JIANG Chunlin JIA Weijia GU Ke XU Ning | 2013 | Chinese Journal of Electronics2013，22，2： | 2 |
| 19 | Environmental efficiency analysis of power industry in China based on an entropy SBM model显示文摘 | Yan Zhou Xinpeng Xing Kuangnan Fang Dapeng Liang Chunlin Xu | 2012 | Energy Policy2012，，： | 2 |
| 20 | Multi-omics analysis reveals gut microbiota-induced intramuscular fatdeposition via regulating expression of lipogenesis-associated genes显示文摘The gut microbiome has great effects on the digestion, absorption, and metabolism of lipids. However,the microbiota composition that can alter the fat deposition and the meat quality of pigs remains unclear.Here, we used Laiwu (LW) pigs (a native Chinese breed with higher intramuscular fat) compared withcommercial crossbreed Duroc×(Landrace×Yorkshire) (DLY) pigs to investigate the effects of microbiotaon meat quality, especially in intramuscular fat content. A total of 32 DLY piglets were randomly allottedto 4 groups and transplanted with fecal microbiota from healthy LW pigs. The results indicated that thehigh dose of fecal microbiota transplantation (HFMT) selectively enhanced fat deposition in longissimusdorsi (P < 0.05) but decreased backfat thickness (P < 0.05) compared with control group. HFMT significantlyaltered meat color and increased feed conversation ratio (P < 0.05). Furthermore, the multi-omicsanalysis revealed that Bacteroides uniformis, Sphaerochaeta globosa, Hydrogenoanaerobacterium saccharovorans,and Pyramidobacter piscolens are the core species which can regulate lipid deposition. A total of140 male SPF C57BL/6j mice were randomly allotted into 7 groups and administrated with these 4 microbesalone or consortium to validate the relationships between microbiota and lipid deposition.Inoculating the bacterial consortium into mice increased intramuscular fat content (P < 0.05) comparedwith control mice. Increased expressions of lipogenesis-associated genes including cluster of differentiation36 (Cd36), diacylglycerol O-acyltransferase 2 (Dgat2), and fatty acid synthase (FASN) wereobserved in skeletal muscle in the mice with mixed bacteria compared with control mice. Together, ourresults suggest that the gut microbiota may play an important role in regulating the lipid deposition in the muscle of pigs and mice. | Chunlin Xie Junyong Teng Xinkai Wang Baoyang Xu Yaorong Niu Libao Ma Xianghua Yan | 2022 | Animal Nutrition2022，，2： | 2 |
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