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| 1 | Desferrioxamine in warm reperfusion media decreases liver injury aggravated by cold storage显示文摘AIM:To evaluate whether desferrioxamine decreases ischemia and perfusion injury aggravated by cold storage(CS)in a rat liver perfusion model. METHODS:Isolated rat livers were kept in CS in University of Wisconsin Solution for 20 h at 4℃,then exposed to 25 min of warm ischemia(WI)at 37℃ followed by 2 h of warm perfusion(WP)at 37℃with oxygenated(95%oxygen and 5%carbon dioxide) Krebs-Henseleit buffer.Desferrioxamine(DFO),an iron chelator,was added at different stages of storage,ischemia and perfusion:in CS only,in WI only,in WP only, in WI and perfusion,or in all stages.Effluent samples were collected after CS and after WI.Perfusate samples and bile were collected every 30 min(0,0.5,1,1.5 and 2 h)during liver perfusion.Cellular injury was assessed by the determination of lactate dehydrogenase(LDH) and aspartate aminotransferase(AST)in the effluent and perfusate samples.Total iron was analysed in the perfusate samples.After WP,the liver was collected for the determination of liver swelling(wet to dry ratio) and liver morphological examination(hematoxylin and eosin staining). RESULTS:Increased CS time caused increased liver dysfunction during WP.After 2 h of WP,liver injury was indicated by increased release of AST(0.5 h CS:9.4± 2.2 U/g liver vs 20 h CS:45.9±10.8 U/g liver,P<0.05) and LDH(0.5 h CS:59±14 U/g liver vs 20 h CS:297 ±71 U/g liver,P<0.05).There was an associated increase in iron release into the perfusate(0.5 h CS:0.11 ±0.03μmoL/g liver vs 20 h CS:0.58±0.10μmoL/g liver,P<0.05)and reduction in bile flow(0.5 h CS: 194±12μL/g vs 20 h CS:71±8μL/g liver,P<0.05). When DFO was added during WI and WP following 20 h of CS,release of iron into the perfusate was de- creased(DFO absent 0.58±0.10μmoL/g liver vs DFO present 0.31±0.06μmoL/g liver,P<0.05),and liver function substantially improved with decreased release of AST(DFO absent 45.9±10.8 U/g liver vs DFO present 8.1±0.9 U/g liver,P<0.05)and LDH(DFO absent 297±71 U/g liver vs DFO present 56±7 U/g liver,P<0.05),and increased bile flow(DFO absent 71±8μL/g liver vs DFO present 237±36μL/g liver, P<0.05).DFO was also shown to improve liver morphology after WP.Cellular injury(the release of LDH and AST)was significantly reduced with the addition of DFO in CS medium but to a lesser extent compared to the addition of DFO in WP or WI and perfusion.There was no effect on liver swelling or bile flow when DFO was only added to the CS medium. CONCLUSION:DFO added during WI and perfusion decreased liver perfusion injury aggravated by extended CS. | Peter G Arthur Xian-Wa Niu Wen-Hua Huang Bastiaan DeBoer Ching Tat Lai Enrico Rossi John Joseph Gary P Jeffrey | 2013 | World Journal of Gastroenterology2013，19，5： | 2 |
| 2 | Effectof mobile phase composition on the separation of propranolol enantiomers using a perphenylcarbamate β-cyclodextrin bonded chiral stationary phase显示文摘 | CHING C B FU P N G SC | 2000 | J Chromatogr A2000，898，： | 2 |
| 3 | Changes in uhrastructure and abscisic acid level and response to applied gibberellins in Taxus rnalrei seeds treated with warm and cold stratification显示文摘 | CHING T C LING L H TSAN P L | 1998 | Annals of Botany1998，81，： | 1 |
| 4 | Determinants of the decision to submit market or limit orders on the ASX 显示文摘 | Verhoeven P Ching S Guan H | 2004 | Pacific-Basin Finance Journal2004，12，1： | 1 |
| 5 | Rooting of Alnus rubra cuttings 显示文摘 | Monaco P A T M Ching K K Ching | 1980 | Tree Planters'' Notes1980，31，3： | 1 |
| 6 | Oxidative degradation of 2, 4, 6-Trinitrotoluene by ozone in an electrohydraulic discharge reactor显示文摘 | Lang P S Ching W K Willberg D M | 1998 | Environ Sci Technol1998，32，20： | 1 |
| 7 | Joint Frequency and Symbol Synchronization Schemes for an OFDM System 显示文摘 | CHING P H | 1999 | Wireless Personal Communications1999，10，： | 1 |
| 8 | Binaural benefits for adults who use hearing aids and cochlear implants in opposite ears显示文摘 | CHING T Y INCERTI P HILL M | 2004 | Ear Hear2004，25，： | 1 |
| 9 | Exact and approximate maximum likelihood localization algorithms显示文摘 | Chan Y T Hang H Y C and Ching P C | 2006 | IEEE Transactions on Vehicular Technology2006，55，1： | 1 |
| 10 | Large-scale analysis of the human and mouse rranscriptomes显示文摘 | SU A I COOKE M P CHING K A | 2002 | Proc Natl Acad Sci USA2002，99，7： | 1 |
| 11 | DNA adduct formation and DNA strand breaks in green-lipped mussels (Perna viridis) exposed to benzo pyrene : Dose-and time-dependent relation- ships显示文摘 | CHING E W SIU W H LAM P K | 2001 | Marine Pollution Bulletin2001，42，： | 1 |
| 12 | A novel blind estimation algorithm显示文摘 | LAI W K CHING P C | 1997 | IEEE Trans Signal Process1997，45，7： | 1 |
| 13 | On wavelet denoising and its application to time delay estimation 显示文摘 | Ching P C So H C Wu S Q | 1999 | IEEE Trans on SP1999，47，10： | 1 |
| 14 | The immunomodulatory effects of lactic acid bacteria for improving immune functions and benefits显示文摘 | Ting T Y Ching C P Ming P T | 2012 | Appl Mierobiol Biotechnol2012，96，： | 1 |
| 15 | Epigenome analyses using BAC microarrays identify evolutionary conservation of tissue-specific methylation of SHANK3显示文摘 | Ching T T Maunakea A K Jun P | 2005 | Nat Genet2005，37，6： | 1 |
| 16 | Ab initio calculation of the electronic structure and spectroscopic properties of spinel gamma-Sn《,3》N《,4》显示文摘 | | 2006 | Physical Review B2006，73，04： | 1 |
| 17 | Wavelet based Approach for Joint Time Delay and Doppler Stretch Measurements显示文摘 | Niu X X Ching P C Chan Y T | 1999 | IEEE Transactions on Aerospace and Electronic Systems1999，35，3： | 1 |
| 18 | Highly efficient light emitting diodes based on fluorene copolymer consisting of triarylamine units in the main chain and oxadiazole pendent groups显示文摘 | Wu Fang Iy Shih P I Shu Ching Fong | 2005 | Macromolecules2005，38，22： | 1 |
| 19 | Identification of a murine CD4+T-lymphocyte response site in hepatitis C virus core protein显示文摘 | Chen Z P Berkower I Ching W M | 1996 | Mol Immunol1996，33，78： | 1 |
| 20 | MiR-145 modulates multiple components of the insulin-like growth factor pathway in hepatocellular carcinoma显示文摘 | Law P T Ching A K Chan A W | | 0，，6： | 1 |
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