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| 1 | Desferrioxamine in warm reperfusion media decreases liver injury aggravated by cold storage显示文摘AIM:To evaluate whether desferrioxamine decreases ischemia and perfusion injury aggravated by cold storage(CS)in a rat liver perfusion model. METHODS:Isolated rat livers were kept in CS in University of Wisconsin Solution for 20 h at 4℃,then exposed to 25 min of warm ischemia(WI)at 37℃ followed by 2 h of warm perfusion(WP)at 37℃with oxygenated(95%oxygen and 5%carbon dioxide) Krebs-Henseleit buffer.Desferrioxamine(DFO),an iron chelator,was added at different stages of storage,ischemia and perfusion:in CS only,in WI only,in WP only, in WI and perfusion,or in all stages.Effluent samples were collected after CS and after WI.Perfusate samples and bile were collected every 30 min(0,0.5,1,1.5 and 2 h)during liver perfusion.Cellular injury was assessed by the determination of lactate dehydrogenase(LDH) and aspartate aminotransferase(AST)in the effluent and perfusate samples.Total iron was analysed in the perfusate samples.After WP,the liver was collected for the determination of liver swelling(wet to dry ratio) and liver morphological examination(hematoxylin and eosin staining). RESULTS:Increased CS time caused increased liver dysfunction during WP.After 2 h of WP,liver injury was indicated by increased release of AST(0.5 h CS:9.4± 2.2 U/g liver vs 20 h CS:45.9±10.8 U/g liver,P<0.05) and LDH(0.5 h CS:59±14 U/g liver vs 20 h CS:297 ±71 U/g liver,P<0.05).There was an associated increase in iron release into the perfusate(0.5 h CS:0.11 ±0.03μmoL/g liver vs 20 h CS:0.58±0.10μmoL/g liver,P<0.05)and reduction in bile flow(0.5 h CS: 194±12μL/g vs 20 h CS:71±8μL/g liver,P<0.05). When DFO was added during WI and WP following 20 h of CS,release of iron into the perfusate was de- creased(DFO absent 0.58±0.10μmoL/g liver vs DFO present 0.31±0.06μmoL/g liver,P<0.05),and liver function substantially improved with decreased release of AST(DFO absent 45.9±10.8 U/g liver vs DFO present 8.1±0.9 U/g liver,P<0.05)and LDH(DFO absent 297±71 U/g liver vs DFO present 56±7 U/g liver,P<0.05),and increased bile flow(DFO absent 71±8μL/g liver vs DFO present 237±36μL/g liver, P<0.05).DFO was also shown to improve liver morphology after WP.Cellular injury(the release of LDH and AST)was significantly reduced with the addition of DFO in CS medium but to a lesser extent compared to the addition of DFO in WP or WI and perfusion.There was no effect on liver swelling or bile flow when DFO was only added to the CS medium. CONCLUSION:DFO added during WI and perfusion decreased liver perfusion injury aggravated by extended CS. | Peter G Arthur Xian-Wa Niu Wen-Hua Huang Bastiaan DeBoer Ching Tat Lai Enrico Rossi John Joseph Gary P Jeffrey | 2013 | World Journal of Gastroenterology2013，19，5： | 2 |
| 2 | Effectof mobile phase composition on the separation of propranolol enantiomers using a perphenylcarbamate β-cyclodextrin bonded chiral stationary phase显示文摘 | CHING C B FU P N G SC | 2000 | J Chromatogr A2000，898，： | 2 |
| 3 | Quality of life and psychological morbidity in women with polycystic ovary syndrome:body mass index,age and the provision of patient information are significant modifiers显示文摘 | Ching H L Burke V Stuckey B G | 2007 | J Clin Endocrinol Metab2007，66，3： | 1 |
| 4 | Efficient evaluation ofreliability for slopes with circular slip surfaces usingimportance sampling 显示文摘 | Ching J Phoon K K Hu Y G | 2009 | Journal of Geotechnical andGeoenvironmental Engineering2009，135，6： | 1 |
| 5 | Introduction to the longitudinal outcomes of children with hearing impairment(LOCHI)study:background,design,sample characteristics显示文摘 | CHING T Y LEIGH G DILLON H | 2013 | Int J Audiol2013，52，： | 1 |
| 6 | Quality of life and psyehologieal morbidity in women with polyeystie ovary syn- drome : body mass index, age and the provision of patien in for- mation are signifieant modifiers 显示文摘 | CHING H L BURKE V STUEKEY B G | 2007 | Clin Endoerinol ( oxf)2007，66，： | 1 |
| 7 | Coupling between the spinal cord and cervical vertebral column under tensile loading显示文摘 | Shannon G Kroeker Randal P Ching | 2013 | Journal of Biomechanics2013，46，4： | 1 |
| 8 | The role of ostiomeatal complex obstruction in maxillary fungus ball显示文摘 | Tsai T L Yuan C G Ching Y H | 2006 | Otolaryngol Head Neck Surg2006，134，3： | 1 |
| 9 | The incidence and management of priapism in Western Australia: a 16 year audit显示文摘 | Earle C M Stuckey B G Ching | 2003 | Int J Impot Res2003，15，： | 1 |
| 10 | Some difficulties associated with the limit equilibriummethod of slices显示文摘 | Ching R K H Fredlund D G | 1983 | Canadian Geotechnical Journal1983，20，4： | 1 |
| 11 | Evaluating the antioxidant status of weanling pigs fed dietary vitamins A and E显示文摘 | Ching S Mahan D C Wiseman T G | 2002 | Journal Animal Science2002，80，： | 1 |
| 12 | Thermal aggregation of globulin from an indigenous Chinese legume,Phaseolus angularis(red bean)显示文摘 | Meng G T Ching K M Ma C Y | 2002 | Food Chemistry2002，79，： | 1 |
| 13 | High-rate full-diversity spacetime-frequency codes for broadband MIMO block-fading channels显示文摘 | Zhang W Xia X G Ching P C | 2007 | IEEE Tran Commun2007，55，1： | 1 |
| 14 | Overexpres-sion of alpha-intemexin causes abnormal neurofilamentous ac-cumulations and motor coordination deficits in transgenic mice显示文摘 | CHING G Y CHIEN C L FLORES R | 1999 | J Neurosci1999，19，8： | 1 |
| 15 | Pediatric HIV-1 specific cytotoxic T-lymphocyte responses suggesting ongoing viral replication despite combination antiretroviral therapy 显示文摘 | Ching N Yang O O Deville J G | 2007 | Peadiatr Res2007，61，6： | 1 |
| 16 | Hypertension 显示文摘 | | 1991 | Postgrad Med J1991，67，785： | 1 |
| 17 | Application of multi-objective programming to water quality management in a river basin显示文摘 | CHIH S L CHING G | 1996 | Journal of Environment Management1996，，47： | 1 |
| 18 | Thermal aggregation of globulin from an indigenous Chinese legume, Phaseolus angularis(reA bean)显示文摘 | MENG G T CHING K M MA C Y | 2002 | Food Chemistry2002，79，： | 1 |
| 19 | Using deficit irrigation approach for evaluating the effects of water restriction on field grown tomato(Lycopersicon esculentum)显示文摘 | LENNOX A G YU M W CHING H H | 2012 | African Journal of Agricultural Research2012，7，14： | 1 |
| 20 | Quality of life and psychological morbidity in women with polyeystic ovary syndrome: body mass index, age and the provision of patient information are significant modifiers显示文摘 | Ching H L Burke V Stuckey B G | 2007 | J Clin Endoerinol Metab2007，66，3： | 1 |
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