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| 1 | CD4^(+) T cell-released exosomes inhibit CD8^(+) cytotoxic T-lymphocyte responses and antitumor immunity显示文摘T cells secrete bioactive exosomes(EXO),but the potential immunoregulatory effect of T-cell EXO is largely unknown.In this study,we generated activated ovalbumin(OVA)-specific CD4^(+) T cells in vitro via coculture of OVA-pulsed dendritic cells(DC_(OVA))with naive CD4^(+) T cells derived from OVA-specific T-cell receptor(TCR)transgenic OTII mice.CD4^(+) T-cell EXO were then purified from the CD4^(+) T-cell culture supernatants by differential ultracentrifugation.CD4^(+) T-cell EXO exhibited the‘saucer’shape that is characteristic of EXO with a diameter between 50 and 100 nm,as assessed by electron microscopy,and contained the EXO-associated proteins LAMP-1,TCR and lymphocyte function associated antigen-1(LFA-1),as determined by western blot.Flow cytometric analysis showed that CD4^(+) T-cell EXO expressed CD4^(+) T-cell markers(CD4,TCR,LFA-1,CD25 and Fas ligand),but to a lesser extent than CD4^(+) T cells.We demonstrated that DC_(OVA) took up CD4^(+) T-cell EXO via peptide/major histocompatibility complex(pMHC)II/TCR and CD54/LFA-1 interactions.OVA-specific CD4^(+) T-cell EXO from OTII mice,but not ConA-stimulated polyclonal CD4^(+) T-cell EXO from wild-type C57BL/6 mice inhibited DC_(OVA)-stimulated in vitro CD4^(+) T-cell proliferation and in vivo CD81 cytotoxic T lymphocyte(CTL)responses and antitumor immunity against OVA-expressing B16 melanoma BL6-10OVA cells.In addition,EXO derived from a T-cell hybridoma cell line,MF72.2D9,expressing an OVA-specific CD4^(+) TCR,had a similar inhibitory effect as OTII CD4^(+) T-cell EXO on CTL-mediated antitumor immunity.Taken together,our data indicate that antigen-specific T-cell EXO may serve as a new type of immunosuppressive reagent for use in transplant rejection and treatment of autoimmune diseases. | Haifeng Zhang Yufeng Xie Wei Li Rajni Chibbar Sidong Xiong Jim Xiang | 2011 | Cellular & Molecular Immunology2011，8，1： | 10 |
| 2 | TLR9 agonist enhances radiofrequency ablation-induced CTL responses, leading to the potent inhibition of primary tumor growth and lung metastasis显示文摘Radiofrequency ablation(RFA)is the most common approach to thermal ablation for cancer therapy.Unfortunately,its efficacy is limited by incomplete ablation,and further optimization of RFA is required.Here,we demonstrate that incubation at 65°C triggers more EG7 tumor cell death by necrosis than treatment at 45°C,and the 65°C-treated cells are more effective at inducing antigen-specific CD8^(+)cytotoxic T lymphocyte(CTL)responses after injection in mice than the 45°C-treated ones.Dendritic cells(DCs)that phagocytose 65°C-treated EG7 cells become mature with upregulated MHCII and CD80 expression and are capable of efficiently inducing effector CTLs in mouse tumor models.RFA(65°C)therapy of EG7 tumors induces large areas of tumor necrosis and stimulates CTL responses.This leads to complete regression of small(~100 mm^(3))tumors but fails to suppress the growth of larger(~350 mm^(3))tumors.The administration of the Toll-like receptor-9(TLR9)agonist unmethylated cytosine-phosphorothioate-guanine oligonucleotide(CpG)to DCs phagocytosing 65°C-treated EG7 cells enhances the expression of MHCII and CD40 on DCs as well as DC-induced stimulation of CTL responses.Importantly,the intratumoral administration of CpG following RFA also increases the frequencies of tumor-associated immunogenic CD11b−CD11c^(+)CD103^(+)DC2 and CD11b+F4/80+MHCII+M1 macrophages and increases CD4^(+)and CD8^(+)T-cell tumor infiltration,leading to enhanced CD4^(+)T cell-dependent CTL responses and potent inhibition of primary RFA-treated or distant untreated tumor growth as well as tumor lung metastasis in mice bearing larger tumors.Overall,our data indicate that CpG administration,which enhances RFA-induced CTL responses and ultimately potentiates the inhibition of primary tumor growth and lung metastasis,is a promising strategy for improving RFA treatment,which may assist in optimizing this important cancer therapy. | Aizhang Xu Lifeng Zhang Jingying Yuan Fatma Babikr Andrew Freywald Rajni Chibbar Michael Moser Wenjun Zhang Bing Zhang Zhaoying Fu Jim Xiang | 2019 | Cellular & Molecular Immunology2019，16，10： | 3 |
| 3 | Genetic transformation of strawberry by Agrobacterium tumefaciens using a leaf disk regeneration system显示文摘 | Narender S. Nehra Ravindra N. Chibbar Kutty K. Kartha Raju S. S. Datla William L. Crosby Cecil Stushnoff | 1990 | Plant Cell Reports1990，，6： | 2 |
| 4 | Nonspecific interstitial pneumonia and usual interstitial pneumonia with mutation in surfactant protein C in familial pulmonary fibrosis 显示文摘 | RAJNI CHIBBAR FRANCIS SHIH MONICA BAGA | 2004 | Modem Pathology2004，17，： | 1 |
| 5 | Physicochemical and structural characteristics of flours and starches from waxy and nowaxy wheats显示文摘 | Abdel - Aal E - S Hucl P Chibbar R N | 2002 | Cereal Chemistry2002，79，3： | 1 |
| 6 | Circadian clock regulation of starch metabolism establishes GBSSI as a major contributor to amylopectin synthesis in Chlamydomonas reinhardtii 显示文摘 | Ral J Colleoni C Wattebted F Dauville D Nempont C Deschamps P Li Z Morell M Chibbar R Purton S | 2006 | Plant Physiology2006，142，1： | 1 |
| 7 | Physicochemi-cal and structural characteristics of flours and starches from waxy andnonwaxy wheats显示文摘 | ABDEL-AAL E S M HUCL P CHIBBAR R N | 2002 | Cereal Chemistry2002，79，3： | 1 |
| 8 | Isolation of a cDNA encoding a granule-bound 152-kilodalton starch-branching enzyme in wheat显示文摘 | B(a)ga M Nair R B Repellin A Scoles G J Chibbar R N | | 0，，： | 1 |
| 9 | Potential taxonomic use of random amplified polymorphic DNA (RAPD):a case study in Brassica显示文摘 | Demeke T Adams R P Chibbar R | 1992 | Theoretical and Applied Genetics1992，84，： | 1 |
| 10 | Potential taxonomic use random amplified polymorphic DNA (RAPD):A case study in Brassica显示文摘 | Demeke T Adams RP Chibbar R | 1992 | Theoretical and Applied Genetics1992，84，： | 1 |
| 11 | Advances in cereal functional genomics 显示文摘 | Appels R Francki M Chibbar R | 2003 | Functional and Integrative Genomics2003，3124，： | 1 |
| 12 | Genetic transformation of strawberry by Agrobacterium tumefaciens using a leaf disk regeneration system显示文摘 | Nehra N S Chibbar R N Kartha K K | 1990 | Plant Cell Reports1990，9，： | 1 |
| 13 | Advances m cereal functional genomics 显示文摘 | APPLES R FRANCKI CHIBBAR R | 2003 | Functional g Integrative Genomics2003，3，1： | 1 |
| 14 | Potential taxonomic use of random amplifiedpolymorphic DNA(RAPD): A case study in Brassica显示文摘 | Demeke T Adems R P Chibbar R | 1992 | Theor Appl Genet1992，84，： | 1 |
| 15 | Chromosome-mediated and direct gene transfers in wheat 显示文摘 | Jauhar P P Chibbar R N | 1999 | Genome1999，42，： | 1 |
| 16 | N Molecular cloning and expression analysisof peroxidase genes from wheat显示文摘 | Baga M Chibbar R | 1995 | Plant Molecular Biology1995，，29： | 1 |
| 17 | Moisture and salinity stress induced changes in biochemical constituents and water relations of different grape rootstock cultivars显示文摘 | JOGAIAH S RAMTEKE S D SHARMA J UPADHYAY A K CHIBBAR R N | 2014 | International Journal of Agronomy2014，2014，1： | 1 |
| 18 | Isolation,characterization,and expression analysis of starch synthase Ⅱa cDNA from wheat(Triticum aestivum L )显示文摘 | Gao M Chibbar R N | | 0，，： | 1 |
| 19 | Transformation of white spruce (Picea glauca) somatic embryos by microprojectile bombardment显示文摘 | V. R. Bommineni R. N. Chibbar R. S. S. Datla E. W. T. Tsang | 1993 | Plant Cell Reports1993，，1： | 1 |
| 20 | Chromosome-mediated and direct gene transfers in wheat显示文摘 | Jauhar P P Chibbar R N | 1999 | Genome1999，42，4： | 1 |
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