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| 1 | Interrogation of Streptomyces avermitilis for efficient production of avermectins显示文摘The 2015 Nobel Prize in Physiology or Medicine has been awarded to avermectins and artemisinin,respectively.Avermectins produced by Streptomyces avermitilis are excellent anthelmintic and potential antibiotic agents.Because wild-type strains only produce low levels of avermectins,much research effort has focused on improvements in avermectin production to meet the ever increasing demand for such compounds.This review describes the strategies that have been widely employed and the future prospects of synthetic biology applications in avermectin yield improvement.With the help of genome sequencing of S.avermitilis and an understanding of the avermectin biosynthetic/regulatory pathways,synthetic and systems biotechnology approaches have been applied for precision engineering.We focus on the design and synthesis of biological chassis,parts,devices,and modules from diverse microbes to reconstruct and optimize their dynamic processes,as well as predict favorable effective overproduction of avermectins by a 4Ms strategy(Mine,Model,Manipulation,and Measurement). | Jinsong Chen Mei Liu Xueting Liu Jin Miao Chengzhang Fu Heyong Gao Rolf Muller Qing Zhang Lixin Zhang | 2016 | Synthetic and Systems Biotechnology2016，1，1： | 10 |
| 2 | Inactivation of SACE_3446, a TetR family transcriptional regulator, stimulates erythromycin production in Saccharopolyspora erythraea显示文摘Erythromycin A is a widely used antibiotic produced by Saccharopolyspora erythraea;however,its biosynthetic cluster lacks a regulatory gene,limiting the yield enhancement via regulation engineering of S.erythraea.Herein,six TetR family transcriptional regulators(TFRs)belonging to three genomic context types were individually inactivated in S.erythraea A226,and one of them,SACE_3446,was proved to play a negative role in regulating erythromycin biosynthesis.EMSA and qRT-PCR analysis revealed that SACE_3446 covering intact N-terminal DNA binding domain specifically bound to the promoter regions of erythromycin biosynthetic gene eryAI,the resistant gene ermE and the adjacent gene SACE_3447(encoding a longchain fatty-acid CoA ligase),and repressed their transcription.Furthermore,we explored the interaction relationships of SACE_3446 and previously identified TFRs(SACE_3986 and SACE_7301)associated with erythromycin production.Given demonstrated relatively independent regulation mode of SACE_3446 and SACE_3986 in erythromycin biosynthesis,we individually and concomitantly inactivated them in an industrial S.erythraea WB.Compared with WB,the WBΔ3446 and WBΔ3446Δ3986 mutants respectively displayed 36%and 65%yield enhancement of erythromycin A,following significantly elevated transcription of eryAI and ermE.When cultured in a 5 L fermentor,erythromycin A ofWBΔ3446 and WBΔ3446Δ3986 successively reached 4095 mg/L and 4670 mg/L with 23%and 41%production improvement relative to WB.The strategy reported here will be useful to improve antibiotics production in other industrial actinomycete. | Hang Wu Yansheng Wang Li Yuan Yongrong Mao Weiwei Wang Lin Zhu Panpan Wu Chengzhang Fu Rolf Muller David T.Weaver Lixin Zhang Buchang Zhang | 2016 | Synthetic and Systems Biotechnology2016，1，1： | 7 |
| 3 | Pt3Ag alloy wavy nanowires as highly effective electrocatalysts for ethanol oxidation reaction显示文摘Direct ethanol fuel cell(DEFC)has received tremendous research interests because of the more convenient storage and transportation of ethanol vs.compressed hydrogen.However,the electrocatalytic ethanol oxidation reaction typically requires precious metal catalysts and is plagued with relatively high over potential and low mass activity.Here we report the synthesis of Pt3Ag alloy wavy nanowires via a particle attachment mechanism in a facile solvothermal process.Transmission microscopy studies and elemental analyses show highly wavy nanowire structures with an average diameter of 4.6±1.0 nm and uniform Pt3Ag alloy formation.Electrocatalytic studies demonstrate that the resulting alloy nanowires can function as highly effective electrocatalysts for ethanol oxidation reactions(EOR)with ultrahigh specific activity of 28.0 mA/cm^2 and mass activity of 6.1 A/mg,far exceeding that of the commercial Pt/carbon samples(1.10 A/mg).The improved electrocatalytic activity may be partly attributed to partial electron transfer from Ag to Pt in the Pt3Ag alloy,which weakens CO binding and the CO poisoning effect.The one-dimensional nanowire morphology also contributes to favorable charge transport properties that are critical for extracting charge from catalytic active sites to external circuits.The chronoamperometry studies demonstrate considerably improved stability for long term operation compared with the commercial Pt/C samples,making the Pt3Ag wavy nanowires an attractive electrocatalyst for EOR. | Xiaoyang Fu Chengzhang Wan Aixin Zhang Zipeng Zhao Huaixun Huyan Xiaoqing Pan Shuaijing Du Xiangfeng Duan Yu Huang | 2020 | Nano Research2020，13，5： | 5 |
| 4 | Ultrathin wavy Rh nanowires as highly effective electrocatalysts for methanol oxidation reaction with ultrahigh ECSA显示文摘Direct methanol fuel cells (DMFCs) have received tremendous research interests because of the facile storage of liquid methanol vs.hydrogen.However,the DMFC today is severely plagued by the poor kinetics and rather high overpotential in methanol oxidation reaction (MOR).Here we report the investigation of the ultrathin Rh wavy nanowires as a highly effective MOR electrocatalyst.We show that ultrathin wavy Rh nanowires can be robustly synthesized with 2-3 nm diameters.Electrochemical studies show a current peak at the potential of 0.61 V vs.reversible hydrogen electrode (RHE),considerably lower than that of Pt based catalysts (~ 0.8-0.9 V vs.RHE).Importantly,with ultrathin diameters and favorable charge transport,the Rh nanowires catalysts exhibit an ultrahigh electrochemically active surface area determined from CO-stripping (ECSAco) of 144.2 m2/g,far exceeding that of the commercial Rh black samples (20 m2/g).Together,the Rh nanowire catalysts deliver a mass activity of 722 mA/mg at 0.61 V,considerably higher than many previously reported electrocatalysts at the same potential.The chronoamperometry studies also demonstrate good stability and CO-tolerance compared with the Rh black control sample,making ultrathin Rh wavy nanowires an attractive electrocatalyst for MOR. | Xiaoyang Fu Zipeng Zhao Chengzhang Wan Yiliu Wang Zheng Fan Frank Song Bocheng Cao Mufan Li Wang Xue Yu Huang Xiangfeng Duan | 2019 | Nano Research2019，12，1： | 3 |
| 5 | Anesthesia Management of Living RelatedLiver Transplantation(A case report)显示文摘Objective To investigate the effects of anesthesia management on living related liver transplantation. Methods The donor was given procaine fentanyl balanced anesthesia; the recipient was given thiopentol fentanyl atracurium intravenous balanced anesthesia. ECG, P ET CO 2,SpO 2, blood gas, glucose and potassium were monitored. Results Cardiovascular system and respiratory system of the recipient during operation were stable. Conclusion Fentanyl intravenous balanced anesthesia may be used to manage living related liver transplantion. But, the following problems shoud be paid to attention. ①Citrate and lactic acid might accumulate causing metabolic acidosis. ②Hyperkalemia might be developed as the donated liver was reperfused. ③As the liver was an important place for glycogenesis, glycogenolysis, blood glucose was carefully observed and insulin was given to the liver transplantation patient.④As the living related liver was translated, reperfusion syndrome could develop.⑤ The patient was monitored carefully postoperation. Analgesic was given if needed. | Fu Chengzhang(傅诚章) Gao Qiaolan(高巧兰) Liu Cunming(刘存明) Zhang Guolou (张国楼) Zhuang Chanfen (庄蟾芬) Department of Anesthesiology, the First Affiliated Hospital, Nangjing Medical University,Nanjing 210029, P.R.China | 2000 | Journal of Nanjing Medical University2000，14，1： | 0 |
| 6 | Anesthetic considerations for the patients with off-pump coronary artery bypass grafting显示文摘 | Xinchun Chen Chengzhang Fu Chris Cokis | 2005 | Journal of Nanjing Medical University2005，19，5： | 0 |
| 7 | Effects of aerosolized ketamine on the level of nitric oxide and nitric oxide synthetase in the lung tissue of rat with asthma显示文摘Objective: To explore the effects of aerosolized ketamine on the level of nitric oxide and nitric oxide synthetase in the lung tissue in rat asthma model. Methods: Forty SD rats were randomly assigned to five groups: control group (group N), asthma model group (group A), two pretreated groups of different concentrations of ketamine (group K1, K2) and dexamethasone group(group D) with eight rats in each group.The rats in group A were sensitized by injection of ovalbumin (OA) together with aluminum hydroxide and bordetella pertussis as adjuvants. Two weeks after the sensitization,aerosolized OA was used to cause asthma. The rats in group K1 and K2 were sensitized with OA as group A , and then exposed to aerosol of ketamine ,with the concentration of 25 g/L and 50 g/L respectively.Before using aerosolized OA,the rats in group D were exposed to aerosol of 0.01% dexamethasone .The level of NO2-/NO3- in lung tissues, inducible nitric oxide synthetase(iNOS) and constitute nitric oxide synthetase(cNOS) was measured in all groups. Results: The level of NO2-/NO3- and the activity of iNOS in lung tissues in group A were signiticantly higher than those in the other groups. The iNOS activity and the level of NO2-/NO3- in lung tissues were highly positively correlated. Conclusion: NO can induce airway hyperreactivity that may worsen asthma. Aerosolized ketamine can decrease the iNOS expression and reduce the level of NO in the lung tissue in rat asthma model. | Weiqian Tian Chengzhang Fu Minmin Zhu Haixia Li | 2006 | Journal of Nanjing Medical University2006，20，2： | 0 |
| 8 | The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function,atrio-ventricular node function and hemodynamics显示文摘Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the follow-ing data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT) ,index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency(WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after adminis-tration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P < 0.01);the MAP decreased(P < 0.01) and the HR increased drastically(P < 0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg) nor nicardipine(10μg/kg,20μg/kg) has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients. | Jingming Zhu Ningning Hui Xiuhong Jiang Huaxing Zhang Chengzhang Fu | 2007 | Journal of Nanjing Medical University2007，21，6： | 0 |
| 9 | Nobachelins,new siderophores from Nocardiopsis baichengensis protecting Caenorhabditis elegans from Pseudomonas aeruginosa infection显示文摘The biosynthetic potential of actinobacteria to produce novel natural products is still regarded as immense.In this paper,we correlated a cryptic biosynthetic gene cluster to chemical molecules by genome mining and chemical analyses,leading to the discovery of a new group of catecholate-hydroxamate siderophores,nobachelins,from Nocardiopsis baichengensis DSM 44845.Nobachelin biosynthesis genes are conserved in several bacteria from the family Nocardiopsidaceae.Structurally,nobachelins feature fatty-acylated hydroxy-ornithine and a rare chlorinated catecholate group.Intriguingly,nobachelins rescued Caenorhabditis elegans from Pseudomonas aeruginosa-mediated killing. | Haowen Zhao Yuhao Ren Feng Xie Huanqin Dai Hongwei Liu Chengzhang Fu Rolf Müller | 2023 | Synthetic and Systems Biotechnology2023，8，4： | 0 |
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