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| 1 | Effect of magnesium ions/Type I collagen promote the biological behavior of osteoblasts and its mechanism显示文摘Type I collagen(Col I)is a main component of extracellular matrix(ECM).Its safety,biocompatibility,hydrophilicity and pyrogen immunogenicity make it suitable for tissues engineering applications.Mg2t also control a myriad of cellular processes,including the bone development by enhancing the attachment and differentiation of osteoblasts and accelerating mineralization to enhance bone healing.In our studies,Mg2t bind collagen to promote the proliferation and differentiation of osteoblasts through the expression of integrins and downstream signaling pathways.In order to clarify the biological behavior effect of 10mM Mg2t/Col I coating,we performed 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT),alkaline phosphatase(ALP),406-diamidino-2-phenylindole(DAPI),Alizarin red staining and Rhodamine B-isothiocyanate(RITC)-labeled phalloidin experiments and found that 10mM Mg2t group,Col I-coating group,10mM Mg2t/Col I-coating group,respectively,promoted the proliferation and differentiation of osteoblasts,especially 10mM Mg2t/Col I-coating group.We detected the mRNA expression of osteogenic-related genes(Runx2,ALP and OCN,OPN and BMP-2)and the protein expression of signaling pathway(integrin a2,integrin b1,FAK and ERK1/2),these results indicated that 10mM Mg2t/Col I coating play an critical role in up-regulating the MC3T3-E1 cells activity.The potential mechanisms of this specific performance may be through activating via integrin a2b1-FAK-ERK1/2 protein-coupled receptor pathway. | Xiaojing Nie Xirao Sun Chengyue Wang Jingxin Yang | 2020 | Regenerative Biomaterials2020，7，1： | 8 |
| 2 | Effect of loading rate on shear strength parameters of mechanically and biologically treated waste显示文摘Mechanical biological treatment(MBT)technology has attracted increasing attention because it can reduce the volume of waste produced.To deal with the current trend of increasing waste,MBT practices are being adopted to address waste generated in developing urban societies.In this study,a total of 20 specimens of consolidated undrained triaxial tests were conducted on waste obtained from the Hangzhou Tianziling landfill,China,to evaluate the effect of loading rate on the shear strength parameters of MBT waste.The MBT waste samples exhibited an evident strain-hardening behavior,and no peak was observed even when the axial strain exceeded 25%.Further,the shear strength increased with an increase in the loading rate;the effect of loading rate on shear strength under a low confining pressure was greater than that under a high confining pressure.Furthermore,the shear strength parameters of MBT waste were related to the loading rate.The relationship between the cohesion,internal friction angle,and logarithm of the loading rate could be fitted to a linear relationship,which was established in this study.Finally,the ranges of shear strength parameters cohesion c and effective cohesion c′were determined as 1.0–8.2 kPa and 2.1–14.9 kPa,respectively;the ranges of the internal friction angleφand effective internal friction angleφ’were determined as 16.2°–29°and 19.8°–43.9°,respectively.These results could be used as a valuable reference for conducting stability analyses of MBT landfills. | Guoyang Fan Zhenying Zhang Jiahe Zhang Jiayue Zhang Qiaona Wang Min Wang Bang Wang Chengyu Nie | 2022 | Frontiers of Environmental Science & Engineering2022，16，12： | 0 |
| 3 | Rational design of stretchable and conductive hydrogel binder for highly reversible SiP2 anode显示文摘The emerging SiP2with large capacity and suitable plateau is proposed to be the alternative anode for Li-ion batteries.However,typical SiP2still suffers from serious volume expansion and structural destruction,resulting in much Li-consumption and capacity fading.Herein,a novel stretchable and conductive Li-PAA@PEDOT:PSS binder is rationally designed to improve the cyclability and reversibility of SiP2.Interestingly,such Li-PAA@PEDOT:PSS hydrogel enables a better accommodation of volume expansion than PVDF binder(e.g.5.94% vs.68.73% of expansivity).More specially,the SiP2electrode with LiPAA@PEDOT:PSS binder is surprisingly found to enable unexpected structural recombination and selfhealing Li-storage processes,endowing itself with a high initial Coulombic efficiency(ICE) up to 93.8%,much higher than PVDF binder(ICE=70.7%) as well.Such unusual phenomena are investigated in detail for Li-PAA@PEDOT:PSS,and the possible mechanism shows that its Li-PAA component enables to prevent the pulverization of SiP2nanoparticles while the PEDOT:PSS greatly bridges fast electronic connection for the whole electrode.Consequently,after being further composited with carbon matrix,the SiP2/C with LiPAA@PEDOT:PSS hydrogel exhibits high reversibility(ICE> 93%),superior cyclability(>450 cycles),and rate capability(1520 mAh/g at 2000 mA/g) for LIBs.This highly stretchable and conductive binder design can be easily extended to other alloying materials toward advanced energy storage. | Xuhao Liu Runzhe Yao Siqi Wang Yaqing Wei Bin Chen Wei Liang Caiyun Tian Chengyu Nie De Li Yong Chen | 2023 | Journal of Energy Chemistry2023，，8： | 0 |
| 4 | Reduction-responsive nucleic acid nanocarrier-mediated miR-22 inhibition of PI3K/AKT pathway for the treatment of patient-derived tumor xenograft osteosarcoma显示文摘miRNAs are important regulators of gene expression and play key roles in the development of cancer, including osteosarcoma. During the development of osteosarcoma, the expression of miR-22 is significantly downregulated, making miR-22 as a promising therapeutic target against osteosarcoma. To design and fabricate efficient delivery carriers of miR-22 into osteosarcoma cells, a hydroxyl-rich reduction-responsive cationic polymeric nanoparticle, TGIC-CA (TC), was developed in this work, which also enhanced the therapeutic effects of Volasertib on osteosarcoma. TC was prepared by the ring-opening reaction between amino and epoxy groups by one-pot method, which had the good complexing ability with nucleic acids, reduction-responsive degradability and gene transfection performance. TC/miR-22 combined with volasertib could inhibit proliferation, migration and promote apoptosis of osteosarcoma cells in vitro. The anti-tumor mechanisms were revealed as TC/ miR-22 and volasertib could inhibit the PI3K/Akt signaling pathway synergistically. Furthermore, this strategy showed outstanding tumor suppression performance in animal models of orthotopic osteosarcoma, especially in patient-derived chemo-resistant and chemo-intolerant patient-derived xenograft (PDX) models, which reduced the risk of tumor lung metastasis and overcame drug resistance. Therefore, it has great potential for efficient treatment of metastasis and drug resistance of osteosarcoma by the strategy of localized, sustained delivery of miR-22 using the cationic nanocarriers combined with non-traditional chemotherapy drugs. | Dafu Chen Chengyue Lei Weifeng Liu Meiyu Shao Meizhou Sun Jianxun Guo Jingjing Cao Jing-Jun Nie Peng Luo Yuwen Luo Bingran Yu Renxian Wang Shun Duan Fu-Jian Xu | 2023 | Bioactive Materials2023，，10： | 0 |
| 5 | Application of Tobacco Leaves from Shandong in ' Taishan' Brand显示文摘In view of the fact that the proportion of tobacco leaves from Shandong in the stocks of a company is large,based on the premise of not affecting the quality of cigarettes,through the scientific evaluation,selection and matching test,the proportion of tobacco leaves from Shandong in the formula of ' Taishan' cigarette A has been increased,and the sensory quality of ' Taishan' cigarette A before and after the test is basically stable.Moreover,the stocks of tobacco leaves from Shandong can be decreased in time. | Chengyu QIU Xiansheng ZHOU Cuishu LENG Shoujie NIE Guangyu MENG | 2018 | Agricultural Biotechnology2018，7，6： | 0 |
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