|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Therapeutic effect of acupuncture and massage for shoulder-hand syndrome in hemiplegia patients:a clinical two-center randomized controlled trial显示文摘OBJECTIVE:To evaluate the therapeutic effects of acupuncture and massage for shoulder-hand syndrome in hemiplegia patients.METHODS:One hundred and twenty hemiplegia patients with stage I shoulder-hand syndrome were randomly divided into a group treated with standardized electric acupuncture and massage,and a group treated with rehabilitation therapy for 6 weeks.The primary indices evaluated were pain on passive movement of the shoulder using the numeric pain rating scale(NPRS),and the number of patients with shoulder-hand syndrome at Steinbrocker stage II or III after treatment.The secondary indices were Fugl-Meyer evaluation of functional movement of the upper limb and hand using the modified rankin scale(MRS).RESULTS:At post-treatment evaluation and a 12-week follow-up visit,NPRS score,number of patients with stage II or III shoulder-hand syndrome,and MRS score were all improved in the acupuncture-massage group compared with the rehabilitation group(P<0.05).On Fugl-Meyer evaluation,functional movement of the upper limb was also improved in the acupuncture-massage group compared with the rehabilitation group(P<0.05).CONCLUSION:Standardized acupuncture-massage therapy may have curative effects on shoulder-hand syndrome in hemiplegia patients. | Ning Li Fengwei Tian Chengwei Wang Pengming Yu Xi Zhou Qian Wen Xiulan Qiao Lu Huang | 2012 | Journal of Traditional Chinese Medicine2012，32，3： | 25 |
| 2 | Versatile Nucleotides Substitution in Plant Using an Improved Prime Editing System显示文摘Dear Editor,Base editors(BEs)based on the CRISPR/Cas9 system,including cytosine base editors and adenine base editors,which can efficiently perform four transition mutations(C·G-to-T·A and A·T-to-G·C),have been well studied and widely used to produce base mutations in a variety of organisms,including in plants such as rice. | Wen Xu Chengwei Zhang Yongxing Yang Si Zhao Guiting Kang Xiaoqing He Jinling Song Jinxiao Yang | 2020 | Molecular Plant2020，13，5： | 20 |
| 3 | Mushroom Poisoning Outbreaks—China,2010–2020显示文摘ABSTRACT Introduction:Mushroom poisoning was the leading cause of foodborne disease outbreaks and outbreak-associated deaths in China.Mushroom poisoning outbreak surveillance can provide insight into the epidemiological characteristics of mushroom poisonings and guide policymaking and health education to reduce illnesses and deaths.Methods:Foodborne Disease Outbreak Surveillance System was upgraded in 2011 to collect foodborne disease outbreaks in China.Mushroom poisoning outbreaks during 2010–2020 were selected to analyze geographical distribution,seasonal distribution,and setting of food preparation.Results:A total of 10,036 outbreaks,which resulted in 38,676 illnesses and 788 deaths,were reported in this period.Mushroom poisonings occurred all over the country,but with highest incidence in the southwest and central China. | Weiwei Li Sara M.Pires Zhitao Liu Jinjun Liang Yafang Wang Wen Chen Chengwei Liu Jikai Liu Haihong Han Ping Fu Yunchang Guo | 2021 | China CDC weekly2021，3，24： | 18 |
| 4 | Discriminated sgRNAs-Based SurroGate System Greatly Enhances the Screening Efficiency of Plant Base-Edited Cells显示文摘The development of CRISPR/Cas9-mediated base editing has made genomic modification more efficient. However, selection of genetically modified cells from millions of treated cells, especially plant cells, is still challenging. In this study, an efficient surrogate reporter system based on a defective hygromycin resistance gene was established in rice to enrich base-edited cells. After step-by-step optimization, the Discriminated sgRNAs-based SurroGate system (DisSUGs) was established by artificially differentiating the editing abilities of a wild-type single guide RNA (sgRNA) targeting the surrogate reporter gene and an enhanced sgRNA targeting endogenous sites. The DisSUGs enhanced the efficiency of screening base-edited cells by 3- to 5-fold for a PmCDA1-based cytosine-to-tyrosine base editor (PCBE), and 2.5- to 6.5-fold for an adenine base editor (ABE) at endogenous targets. These targets showed editing efficiencies of <25% in the conventional systems. The DisSUGs greatly enhanced the frequency of homozygous substitutions and expanded the activity window slightly for both a PCBE and an ABE. Analyses of the total number of single-nucleotide variants from whole-genome sequencing revealed that, compared with the no-enrichment PCBE strategy, the DisSUGs did not alter the frequency of genome-wide sgRNA-independent off-target mutations, but slightly increased the frequency of target-dependent off-target mutations. Collectively, the DisSUGs developed in this study greatly enhances the efficiency of screening plant base-edited cells and will be a useful system in future applications. | Wen Xu Yongxing Yang Ya Liu Guiting Kang Feipeng Wang Lu Li Xinxin Lv Si Zhao Shuang Yuan Jinling Song Ying Wu Feng Feng Xiaoqing He Chengwei Zhang Wei Song Jiuran Zhao Jinxiao Yang | 2020 | Molecular Plant2020，13，1： | 8 |
| 5 | Developing high-efficiency base editors by combining optimized synergistic core components with new types of nuclear localization signal peptide显示文摘The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base editor(ABE) allow generating precise and irreversible base mutations in a programmable manner and have been used in many different types of cells and organisms. However, their applications are limited by low editing efficiency at certain genomic target sites or at specific target cytosine(C) or adenine(A) residues. Using a strategy of combining optimized synergistic core components, we developed a new multiplex super-assembled ABE(sABE) in rice that showed higher base-editing efficiency than previously developed ABEs. We also designed a new type of nuclear localization signal(NLS) comprising a FLAG epitope tag with four copies of a codon-optimized NLS(F4NLS^(r2)) to generate another ABE named F4NLS-sABE. This new NLS increased editing efficiency or edited additional A at several target sites. A new multiplex super-assembled CBE(sCBE) and F4NLS^(r2) involved F4NLS-sCBE were also created using the same strategy. F4NLS-sCBE was proven to be much more efficient than sCBE in rice. These optimized base editors will serve as powerful genome-editing tools for basic research or molecular breeding in rice and will provide a reference for the development of superior editing tools for other plants or animals. | Feipeng Wang Chengwei Zhang Wen Xu Shuang Yuan Jinling Song Lu Li Jiuran Zhao Jinxiao Yang | 2020 | The Crop Journal2020，8，3： | 3 |
| 6 | Clinicopathological and Prognostic Characteristics of Hepatoid Adenocarcinoma of the Stomach显示文摘 | Jinlin Yang Rui Wang Wenyan Zhang Wen Zhuang Mojin Wang Chengwei Tang D. Fan | 2014 | Gastroenterology Research and Practice2014，，： | 2 |
| 7 | Self-Reported Food Allergy Prevalence Among Elementary School Children—Nanchang City, Jiangxi Province, China, 2021显示文摘What is already known about this topic?The prevalence of food allergy(FA)among the general population has been increasing in recent decades,and seriously affects the physical and mental health and the quality of life of many people—especially children.What is added by this report?The survey estimated self-reported FA prevalence using a standardized FA questionnaire among school children in Nanchang City,Jiangxi Province,China in 2021.What are the implications for public health practice?Based on the local,epidemiological characteristics of food allergy,public policies on the prevention and management of FA should be developed and preventive practices should be promoted to decrease the overall prevalence of FA. | Hua Feng Nan Luo Fang Chen Xinyu Li Yu Wen Chengwei Liu Yan Chen Yongning Wu | 2022 | China CDC weekly2022，4，34： | 1 |
| 8 | Multi-incisional transumbilical laparoscopic surgery for nonpalpable undescended testes: A report of 126 cases显示文摘 | Ning Li Wen Zhang Jiyan Yuan Xuefeng Zhou Xiaojuan Wu Chengwei Chai | 2012 | Journal of Pediatric Surgery2012，，： | 1 |
| 9 | Electrospun nanofiber/hydrogel composite materials and their tissue engineering applications显示文摘Electrospun nanofiber/hydrogel composites combine the excellent biochemical properties of hydrogel with the biomimetic nature of electrospun fibers,and have attracted widespread attention in the last few years.Besides,nanofiber/hydrogel composites with tunable mechanical properties can mimic the microstructure of extracellular matrix(ECM)of various tissues and the microenvironment of different cells.These features enable electrospun fiber/hydrogel composites have uniquely advantageous for tissue repair.However,a comprehensive review of electrospun fiber/hydrogel composites as tissue engineering scaffolds is still lacking.Thus,this article systematically reviewed the preparation of electrospun fiber/hydrogel composites and their application in tissue engineering.First,the preparation strategies of electrospun fiber/hydrogel composites are classified and discussed.Second,the application of electrospun fiber/hydrogel-based scaffolds in tissue engineering,involving skin,blood vessel,nerve,bone and other tissue engineering,are summarized.Finally,future research directions for functional electrospun fiber/hydrogel scaffold materials are proposed. | Miaomiao Zhang Shixin Xu Ruoying Wang Yongan Che Cuicui Han Wei Feng Chengwei Wang Wen Zhao | 2023 | Journal of Materials Science & Technology2023，，31： | 0 |
| 10 | The Hierarchy Mode of Priority Value Orientation in Architectural Design显示文摘 | Na Wang Chengwei Wen | 2017 | Journal of Philosophy Study2017，7，10： | 0 |
| 11 | Analysis of Big Data's Impact on Social Consumption Values显示文摘 | Ming Gao Chengwei Wen | 2018 | Journal of Philosophy Study2018，8，4： | 0 |
| 12 | Research on Chinese Urban Modernity Issues From the Perspective of Technology Rational Critique显示文摘With the development of urbanization,metropolises and megapolises are emerging.Urbanization is being completed with the driving force of capital;meanwhile,technology has achieved the quest of capital.In this thesis,in light of Henri Lefebvre’s ideology of technology rational critique,Chinese urban modernity issues under the perspective of technology rational critique were analyzed,including issues like eroded urban texture,urban mechanization,and urban segregation.The technology rational root of Chinese urban modernity issues was discussed from the turn of technical artifacts from design intention to application intention.In the end,possible methods to solve these Chinese urban modernity issues were illustrated based on Henri Lefebvre’s urban development theory. | ZHAO Yifan WEN Chengwei | 2020 | Journal of Philosophy Study2020，10，9： | 0 |
| 13 | A robust method for identification of plant SUMOylation substrates in a library-based reconstitution system显示文摘Dear Editor,SUMOylation,which transfers a small ubiquitin-like protein to the lysine residues of target proteins,is an important type of posttranslational modification in eukaryotic cells.This modification plays a critical role in plant development and stress responses(Miura and Hasegawa,2010);thus,the efficient identification of SUMOylation substrates in plant cells is a fundamental issue in the field.In Arabidopsis,hundreds of SUMOylated proteins have been identified by a mass spectrometry(MS)approach in which modified targets in transgenic plants harboring an H89R variant of SUMO1 are enriched via affinity chromatography and a four-residue footprint is left on the substrates after trypsin digestion for MS analysis(Miller et al.,2010).Given that antibodies generated against SUMO molecules are not very specific,it is impractical to catch endogenous SUMO moieties via antibody-based one-step purification.Thus,it is difficult to obtain SUMOylation substrates via existing approaches in plant species that cannot be efficiently transformed.Given that SUMOylation is highly reversible(Yates et al.,2016),another problem is that the modification may be removed quickly during sample preparation.In addition,based on the efficiency and sensitivity of affinity enrichment and MS,substrates with low protein levels may be missed in the analyses. | Ruiqiang Lai Wenliang Li Zewei Xu Wen Liu Qianrui Zeng Wenxiong Lin Jieming Jiang Jianbin Lai Chengwei Yang | 2023 | Plant Communications2023，4，4： | 0 |
| 14 | Starved lubrication analysis of angular contact ball bearing based on a multi-degree-of-freedom tribo-dynamic model显示文摘When the oil supply is not adequate to maintain the ideal lubrication,angular contact ball bearing will enter into the starved lubrication regime resulting in the potential performance degradation and consequently the severe failures.To study the effects of starved lubrication on the performance of angular contact ball bearing,this paper first proposes a multi-degree-of-freedom(DOF)tribo-dynamic model by introducing five-DOF inner ring,six-DOF balls,and six-DOF cage.The model considers the starved lubrication in the ball-raceway contact and the full multi-body interactions between the bearing components.With different ball-raceway starvation degrees being analyzed,the effects of starved lubrication on the bearing tribo-dynamic performance are first revealed.By comparison,it is found that the oil film thickness,the skidding performance,and the traction forces in the ball-raceway contact are significantly influenced by the starvation degrees.It is also found that the starvation-induced change of the ball-pocket contact force is dramatical under combined loads,and the maximum contact force under this load condition increases with the increasing starvation degrees. | Chengwei WEN Xianghui MENG Jiaming GU Lin XIAO Shuang JIANG Hanbing BI | 2023 | Friction2023，11，8： | 0 |
| 15 | Electroactive and antibacterial wound dressings based on Ti_(3)C_(2)T_(x)MXene/poly(ε-caprolactone)/gelatin coaxial electrospun nanofibrous membranes显示文摘Endogenous electric fields(EFs)are capable of regulating the behaviors of skin cells in wound healing.However,majority of current dressings are primarily engaged in the passive repair of defective tissue,as they lack the ability to actively respond to physiological electrical signals.In this work,a series of nanofibrous membranes(NFMs)were fabricated by coaxial electrospinning,combining the good mechanical properties of poly(ε-caprolactone)(PCL),the bioactivity of gelatin and the electroactivity of Ti_(3)C_(2)T_(x)MXene,as electroactive and antibacterial dressings for cutaneous wound healing.The obtained NFMs exhibited suitable mechanical properties and hydrophilicity,excellent electroactivity,antibacterial activity,and biocompatibility.Especially,Ti_(3)C_(2)T_(x)MXene/PCL/gelatin-6(MPG-6,6 wt.%of Ti_(3)C_(2)T_(x)MXene in sheath spinning liquids)showed the optimal conductivity and antibacterial activity.Excitingly,this scaffold significantly promoted the adhesion,proliferation,and migration of NIH 3T3 cells under the electrical stimulation(ES).The in vivo evaluation in a full-thickness wounds defect model demonstrated that the MPG-6 films significantly accelerated wound closure,increased granulation tissue formation,increased collagen deposition,and promoted wound vascularization.In summary,the versatile scaffold is expected to be an ideal candidate as wound dressings due to its ability to promote the transmission of physiological electrical signals and thus improved the therapeutic outcomes of wound regeneration. | Shixin Xu Chen Du Miaomiao Zhang Ruoying Wang Wei Feng Chengwei Wang Qinsong Liu Wen Zhao | 2023 | Nano Research2023，16，7： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员