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| 1 | Generation of transgenic wheat resistant to wheat yellow mosaic virus and identification of gene silence induced by virus infection显示文摘The plasmid containing the promoter Actl, the coat protein (cp) gene of wheat yellow mosaic virus (WYMV) and the selectable bar gene, was delivered via particle bombardment, directly into immature embryos of a wheat culti-vars. PCR and PCR-RFLP were employed to screen the existence of the cp gene in T0 and T1 generations. Seeds from the positive T1 plants were sowed in fields heavily contaminated with WYMV to detect their resistance. In field trial of virus infection, one of the transgenic wheat lines, P8-T2, exhibited highly disease-resistance. Western blot and RT-PCR analysis showed that the expression level of cp gene in the resistant transgenic line was reduced greatly compared to those susceptible to WYMV infection. This provided evidence to presume that the resistance obtained by the transgenic wheat line was stimulated by the mechanism of the virus induced gene silencing. | DONG Jin HE Zhentian HAN Chenggui CHEN Xiulan ZHANG Lingdi LIU Weihua HAN Yuepeng WANG Jinrong ZHAI Yafeng YU Jialin LIU Yi XIAO Yueyan | 2002 | Chinese Science Bulletin2002，47，17： | 2 |
| 2 | Isolation and characteriza- tion of YNTC-I, a novel AlicycobaciUus sendaiensis strain显示文摘 | DING/iannan HE Huan ZI-IANG Chenggui | 2008 | J Cent South Univ Technol2008，15，4： | 1 |
| 3 | Constructing backbone network by using tinker algorithm显示文摘 | Zhiwei He Meng Zhan Jianxiong Wang Chenggui Yao | 2017 | Frontiers of physics2017，12，6： | 1 |
| 4 | The effect of oxygen concentration on the coupled neurons:Rich spiking patterns and synchronization显示文摘The dynamical microenvironments play a crucial role in neuronal spiking patterns.In this paper,we investigated the effect of oxygen concentration on different synchronous spiking patterns of two coupled neuron models by including dynamical ion concentration.Two coupling modes of electrical diffusive coupling and potassium diffusive coupling were considered.In these two cases,oxygen concentration exhibited an important role in the synchronous spiking patterns between two coupled neurons,and extremely rich electrical activities were observed.For the potassium diffusive coupling,differential synchronous patterns of oscillation state(OS),synchronous epileptic seizure state(SSZ)and synchronous spreading depression state(SSD)as well as SZ and SD bursting states were generated.For the electrical diffusive coupling,differential synchronous patterns of resting state(RS),SSZ and SSD were observed. | HE ZhiWei YAO ChengGui | 2020 | Science China(Technological Sciences)2020，63，11： | 0 |
| 5 | Impact Failure of Flattened Brazilian Disc with Cracks——Process and Mechanism显示文摘Split Hopkinson pressure bar(SHPB)has been used to study the dynamic failure pattern of flattened mortar Brazilian disc under impact load.Each disc contains several prefabricated cracks paralleled to each other.Dynamic FEM has also been adopted to simulate such failure behavior.The mechanism of crack initiation,propagation and cut-through have been scrutinized with both experimental and numerical approaches.Influence of the number of the prefabricated cracks on the specimen strength and acoustic emission(AE)performance can be observed and studied.The results show that the strength decreases and AE counts increases,when the number of the prefabricated cracks increases. | 张亚芳 刘浩 YIN Guoqi OU Chenggui LU Juan HE Juan | 2020 | Journal of Wuhan University of Technology(Materials Science)2020，35，5： | 0 |
| 6 | A Study of the Factors that Affect Safe Full Heading of Machine-transplanted Rice Seedlings in the Temperate and Cool Rice-growing Areas显示文摘This paper studies the factors that affect the safe full heading of machine-transplanted rice seedlings in the temperate and cool ricegrowing areas,and explores the influence of machine-transplanted rice seedling varieties,seedling age,nitrogen fertilizer application rate and management on the safe full heading of machine-transplanted rice seedlings. Results show that for the mechanized production in the temperate and cool rice-growing areas,we should select the mid-early maturity cold-resistant varieties with growth period of not exceeding 180 d,control the seedling age to be 30- 35 d and total amount of pure nitrogen to be not more than 300 kg / ha,and moderately reduce the nitrogen fertilizer postponing ratio. Under this condition,we can achieve safe full heading of machine-transplanted rice seedlings and ensure the yield. | Yunhua WANG Hongping ZHOU Chenggui HE Taofen PAN Guihua MEI Jimei HUANG | 2016 | Asian Agricultural Research2016，8，11： | 0 |
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