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| 1 | Experimental study of therapeutic efficacy of Baicalin in rats with severe acute pancreatitis显示文摘AIM: To observe the therapeutic efficacy of Baicalin in rats with severe acute pancreatitis (SAP) and explore its therapeutic mechanisms.METHODS: The SAP rat models were randomly divided into the model control group, Baicalin treatment group, octreotide treatment group and sham operation group. All groups were randomly subdivided into 3 h, 6 h and 12 h groups with 15 rats in each group. The survival, ascites volume and pathological changes of pancreas in all rats were observed at different time points after operation. The plasma amylase content and serum TNF-α, IL-6, malonaldehyde (MDA) and PLA2 contents were also determined. RESULTS: The survival was not obviously different between the treated groups, and was significantly higher in treated groups at 12 h compared to the model control group (P < 0.05, 15 vs 10). The ascites/body weight ratio at 3 h and 6 h was significantly lower in Baicalin treatment group compared to the model control group and octreotide treatment group (P < 0.05, 1.00 vs 2.02 and 1.43 and P < 0.001, 2.29 (1.21) vs 2.70 (0.80) and 2.08 (2.21), respectively). The contents of amylase, TNF-α, IL-6, MDA and PLA2 were significantly lower in the treated groups than in the model control group (P < 0.05, 4342 vs 5303, 5058 vs 6272 in amylase, P < 0.01, 21.90 vs 36.30, 23.80 vs 39.70, 36 vs 54.35 in MDA and 56.25 vs 76.10 in PlA2, or P < 0.001, 65.10 and 47.60 vs 92.15 in TNF-α, 3.03 vs 5.44, 2.88 vs 6.82, 2.83 vs 5.36 in IL-6, respectively). The pathological scores of pancreas in the treated groups were significantly lower than that in the model control group (P < 0.05, 9.00 vs 10.05, 6.00 vs 9.00, 8.00 vs 10.05), but no marked difference was found between the treated groups. CONCLUSION: The Baicalin injection has significant therapeutic effects on SAP rats, its effects are similar to those of octreotide. The Baicalin injection is also cheap and has a big application range, quite hopefully to be used in clinical treatment of SAP. | Xi-Ping Zhang Ling Zhang Jin-Xian He Rui-Ping Zhang Qi-Hui Cheng Yi-Feng Zhou Bei Lu | 2007 | World Journal of Gastroenterology2007，13，5： | 23 |
| 2 | Recent advances in g-CN-based heterojunction photocatalysts显示文摘Semiconductor heterojunction photocatalysts have received much concern due to their great application prospect in solar energy utilization and conversion.Rational construction of heterostructure with two or more semiconductor materials can integrate the advantages of multi-components to simultaneously improve the photo-induced charges separation,extend visible light absorption range and retain the high redox ability of photocatalysts.Recently,constructing of g-C3 N4-based heterostructure has become a hot focus due to the multiple merits of g-C3 N4,such as facile synthesis,high stability,unique optical and electronic characteristics.This review tries to give a comprehensive introduction and provide the possible tendency in design and fabrication of g-C3 N4-based heterojunction photocatalysts,especially in rational synthesis of type-II heterojunction,direct Z-scheme heterojunction,new type of S-scheme heterojunction,p-n heterojunction and Schottky heterojunction.Furthermore,the special charges transfer mechanisms of g-C3 N4-based heterojunctions are discussed and their potential applications in photocatalytic water splitting,photo-degradation of contaminants and photo-reduction of CO2 into fuels are also summarized.Finally,we attempt to propose some crucial issues based on the current research,which should be further investigated and resolved in this interesting research area. | Yunfeng Li Minghua Zhou Bei Cheng Yan Shao | 2020 | Journal of Materials Science & Technology2020，56，21： | 16 |
| 3 | 宫颈脱落细胞人乳头瘤病毒(HPV)E6/E7 mRNA检测在HPV阳性女性筛查中的应用(英文)显示文摘目的:基于临床样本探讨宫颈脱落细胞HPV E6/E7mR NA检测能否作为HPV阳性女性的筛查手段。创新点:首次比较了宫颈脱落细胞HPV E6/E7 mR NA检测与宫颈脱落细胞学检测对于HPV阳性女性宫颈活检结果的预测价值。方法:所有入组的HPV阳性女性均进行宫颈脱落细胞学及HPV E6/E7 mR NA检测。比较两种方法对于宫颈活检结果的预测价值。结论:对于HPV阳性的女性,高级别鳞状上皮内病变(HSIL)HPV E6/E7 mR NA阳性率高于低级别鳞状上皮内病变(LSIL)及正常病例。HPV E6/E7mR NA与细胞学检测相比,HSIL及以上级别病变的检测敏感性(89.52%vs.86.67%,P=0.671)、特异性(48.96%vs.48.96%,P=1.000)、阳性预测值(39.00%vs.38.24%,P=1.000)及阴性预测值(92.76%vs.90.97%,P=0.678)均无显著差异。HPV E6/E7 mR NA检测或能成为HPV阳性女性的一种新的筛查手段。 | Ye-li YAO Qi-fang TIAN Bei CHENG Yi-fan CHENG Jing YE Wei-guo LU | 2017 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2017，18，3： | 14 |
| 4 | Prognostic Value of Neutrophil to Lymphocyte Ratio for In-hospital Mortality in Elderly Patients with Acute Myocardial Infarction显示文摘 | Tang-meng GUO Bei CHENG Li KE Si-ming GUAN Ben-ling QI Wen-zhu LI Bin YANG | 2018 | Current Medical Science2018，38，2： | 14 |
| 5 | Factors Associated with Thrombolysis Outcome in Ischemic Stroke Patients with Atrial Fibrillation显示文摘The outcome of early intravenous thrombolysis for ischemic stroke in patients with atrial fibrillation(AF)is worse than that without thrombosis. How to increase the efficacy of intravenous thrombolysis for AF-related ischemic stroke remains largely unknown. In this study, we investigated factors that influence the effect of intravenous thrombolysis in these patients. Our results showed that thrombolysis was independently associated with a favorable outcome(P \ 0.001) and did not influence the mortality of AF-related ischemic stroke, although it increased the risk of hemorrhage within 24 h after treatment. Risk factors for a poor outcome at admission were:heart failure(P = 0.045); high systolic pressure(P = 0.039); high blood glucose(P = 0.030); and a high National Institutes of Health Stroke Scale(NIHSS) score(P \ 0.001). Moreover, high systolic pressure at admission(P = 0.007), high blood glucose(P = 0.027), and a high NIHSS score(P \ 0.001) were independent risk factors for mortality at 3 months. Besides thrombolysis, a high NIHSS score(P = 0.006) and warfarin taken within 48 h before stroke onset(P = 0.032) were also independent risk factors for symptomatic hemorrhage within 24 h after treatment. Ischemic stroke patients with AF benefited from intravenous thrombolysis with recombinant tissue plasminogen activator within 4.5 h after stroke. | Qiuyun Zhao Xiaobo Li Wanli Dong Min Ye Yongjun Cao Meijuan Zhang Qiantao Cheng Junshan Zhou Guofang Chen Ming Yu Shanshan Hong Xiue Wei Bei Wang Guiyun Cui Peng Zhang Hong Ding Rongzhen Xu Yan Chen Yun Xu | 2016 | Neuroscience Bulletin2016，32，2： | 13 |
| 6 | Entecavir add-on Peg-interferon therapy plays a positive role in reversing hepatic fibrosis in treatment-naive chronic hepatitis B patients: a prospective and randomized controlled trial显示文摘Background:The efficacy of entecavir(ETV)add-on peg-interferon therapy compared with ETV monotherapy in treatment-naive hepatitis B virus(HBV)patients remains controversial.We investigated whether adding peg-interferon to ongoing ETV treatment leads to a better curative effect or not.Methods:All patients have been recruited between August 2013 and January 2015 from the Shanghai Public Health Clinical Center and Zhongshan Hospital(China).Eligible HBV patients(n=144)were randomly divided(1:1)to receive either ETV monotherapy(n=70)or peg-interferon add-on therapy from week 26 to 52(«=74).Patients were followed-up for at least 2 years.Indexes including hepatitis B surface antigen(HBsAg)and hepatitis B e antigen(HBeAg)seroconversion rate,sustained virologic response,transient elastography value,and histological scores were evaluated every 3 months until the end of the study.The rate of patients with HBsAg loss was defined as the primary endpoint criteria.Results:At week 26,no patient achieved HBsAg seroconversion in either group.At week 52,one patient in the monotherapy group was HBsAg-negative but there was none in the combination therapy group.The monotherapy group showed significantly better liver function recovery results than the combination therapy group.At week 78,one patient in the combination group had HBsAg seroconverted.At week 104,only three patients in the combination therapy group were HBsAg-negative compared with one patient in monotherapy.The mean alanine aminotransferase and aspartate aminotransferase levels and transient elastography values decreased significantly compared with baseline.Both groups showed a favorable decrease in alpha-fetoprotein(monotherapy:4.5[2.8,7.1]vs.2.2[1.8,3.1]ng/mL,P<0.001;combination therapy:5.7[3.0,18.8]vs.3.2[2.0,4.3]ng/mL,P<0.001)and an improved result of liver biopsy examination scores.The combination group showed a better improvement in histology compared with the monotherapy group(mean transient elastography value 6.6[4.9,9.8]vs.7.8[5.4,11.1]kPa,P=0.028).But there was no significant difference in HBsAg conversion rate(1.8%[1/56]i^s.4.1%[3/73],P=0.809)and HBeAg conversion rate(12.5%[7/56]i/s.11.0%[8/73],P=0.787),as well as HBV-DNA,sustained virologic response(93.2%vs.98.5%,P=0.150)between the two groups.Conclusions:Both therapies supported liver function recovery and histology improvement.Combination therapy did not show better anti-viral efficacy in HBsAg or HBeAg seroconversion compared with monotherapy.However,combination therapy played a more positive role in reversing hepatic fibrosis compared with monotherapy.Trial registration:ClinicalTrials.gov:NCT02849132;http://gffzzd3f38a9638994581spnfw6bkqqfkc6kwb.ffgz.tsg.suse.edu.cn/ct2/show/NCT02849132. | Jing-Mao Yang Li-Ping Chen Ya-Jie Wang Bei Lyu Hong Zhao Zhi-Yin Shang Jun Li Zhen-Yu Fan Sheng-Di Wu Xiao Ming Xian Li Shao-Ping Huang Ji-Lin Cheng | 2020 | Chinese Medical Journal2020，，14： | 11 |
| 7 | Hierarchically nanostructured porous TiO_2(B) with superior photocatalytic CO_2 reduction activity显示文摘Hierarchically nanostructured, porous TiO_2(B) microspheres were synthesized by a microwave-assisted solvothermal method combined with subsequent heat treatment in air. The materials were carefully characterized by scanning and transmission electron microscopy, X-ray diffraction, CO_2 adsorption, and a range of spectroscopies, including Raman, infrared, X-ray photoelectron and UV-Vis spectroscopy. The hierarchical TiO_2(B) particles are constructed by ultrathin nanosheets and possess large specific surface area, which provided many active sites for CO_2 adsorption as well as CO_2 conversion. The TiO_2(B)nanostructures exhibited marked photocatalytic activity for CO_2 reduction to methane and methanol. Anatase TiO_2 and P25 were used as the reference photocatalysts. Transient photocurrent measurement also proved the higher photoactivity of TiO_2(B) than that of anatase TiO_2. In-situ infrared spectrum was measured to identify the intermediates and deduce the conversion process of CO_2 under illumination over TiO_2(B) photocatalyst. | Tingmin Di Jinfeng Zhang Bei Cheng Jiaguo Yu Jingsan Xu | 2018 | Science China Chemistry2018，61，3： | 8 |
| 8 | Review on DFT calculation of s-triazine-based carbon nitride显示文摘To improve the photocatalytic performance of pristine photocatalysts,element doping,construction of composites and fabrication of novel nanostructures are recognized as universal modification methods.These methods have been experimentally verified to be effective in manifold photocatalytic application over various photocatalysts.Density functional theory(DFT)calculation is a powerful and fundamental tool to pinpoint the intrinsic mechanism of the enhanced photocatalytic activity.And it holds the degree of precision ranging from atoms,molecules to unit cells.Herein,recent DFT calculation research progress of modified s-triazine-based graphitic carbon nitride(g-C3N4)systems as photocatalysts is summarized.To specify,we collected information of doping site,formation energy,geometric,and electronic properties.We also discussed the synergistic effect of work function,Fermi level and band edge position on the built-in electric field,transfer route of photogenerated charge carriers and photocatalytic mechanism(traditional typeⅡor direct Z-scheme heterostructure).Moreover,we analyzed the geometric configuration,band structure,and stability of g-C3N4 nanocluster,nanoribbon,and nanotube.Finally,future perspective in the further theoretical revelation of g-C3N4-based photocatalysts is proposed. | Bicheng Zhu Bei Cheng Liuyang Zhang Jiaguo Yu | 2019 | Carbon Energy2019，1，1： | 8 |
| 9 | Evaluation of Vaporized Hydrogen Peroxide Fumigation as a Method for the Bio-decontamination of the High Efficiency Particulate Air Filter Unit显示文摘Objective To evaluate the performance of vaporized hydrogen peroxide (VHP) for the bio-decontamination of the high efficiency particulate air (HEPA) filter unit. Methods Self-made or commercially available bioindicators were placed at designated locations in the HEPA filter unit under VHP fumigation. The spores on coupons were then extracted by 0.5 h submergence in eluent followed by 200-time violent knocks. Results Due to the presence of HEPA filter in the box, spore recovery from coupons placed at the bottom of the filter downstream was significantly higher than that from coupons placed at the other locations. The gap of decontamination efficiency between the top and the bottom of the filter downstream became narrower with the exposure time extended. The decontamination efficiency of the bottom of the filter downstream only improved gently with the injection rate of H2O2 increased and the decontamination efficiency decreased instead when the injection rate exceeded 2.5 g/min. The commercially available bioindicators were competent to indicate the disinfection efficiency of VHP for the HEPA filter unit. Conclusion The HEPA filter unit is more difficult than common enclosure to decontaminate using VHP fumigation. Complete decontamination can be achieved by extending fumigation time. VHP fumigation can be applied for in-situ biodecontamination of the HEPA filter unit as an alternative method to formaldehyde fumigation. | JIA Hai Quan LI Yan Ju SUN Bei ZHAO Si Qing YI Ying ZHAO Ming ZHANG Zong Xing PAN Xin QI Jian Cheng | 2013 | Biomedical and Environmental Sciences2013，26，2： | 8 |
| 10 | Expressions of TGF-β2, bFGF and ICAM-1 in lens epithelial cells of complicated cataract with silicone oil tamponade显示文摘AIM: To investigate the expression differences of transforming growth factor-β2(TGF-β2), basic fibroblast growth factor(b FGF) and intercellular cell-adhesion molecule-1(ICAM-1) in lens epithelial cells(LECs) of complicated cataract with silicone oil tamponade and agerelated cataract. METHODS: Totally 150 eyes of 150 patients(aged 35 to 77y) were investigated, including 75 patients with complicated cataract after silicone oil tamponade and 75 patients with age-related cataract. The central piece of anterior capsules was collected during cataract surgery. TGF-β2, b FGF and ICAM-1 were detected in the 60 specimens of the two groups by immunohistochemistry. The expression levels of the three kinds of messenger ribonucleic acid(m RNA) were determined by real-time quantitative reverse transcriptionpolymerase chain reaction in the 90 specimens of the two groups.RESULTS: TGF-β2 was detected in the cytomembrane and cytoplasm of the LECs and b FGF was detected in the nucleus. ICAM-1 was positive in the cytomembrane of the LECs and the distribution of positive cells was uneven. The m RNA genes expression of the TGF-β2, b FGF and ICAM-1 was significant differences between the two groups and markedly increased in complicated cataract group(P<0.05).CONCLUSION: The up-regulated TGF-β2, b FGF and ICAM-1 maybe associate with the occurrence and development of complicated cataract with silicone oil tamponade. | Bei Liu Jing Gao Bo-Chang Lyu Shan-Shuang Du Cheng Pei Zhong-Qiao Zhu Bo Ma | 2017 | International Journal of Ophthalmology(English edition)2017，10，7： | 7 |
| 11 | Regulation of the intestinal microbiota: An emerging therapeutic strategy for inflammatory bowel disease显示文摘The rapid development of metagenomics,metabolomics,and metatranscriptomics provides novel insights into the intestinal microbiota factors linked to inflammatory bowel disease(IBD).Multiple microorganisms play a role in intestinal health;these include bacteria,fungi,and viruses that exist in a dynamic balance to maintain mucosal homeostasis.Perturbations in the intestinal microbiota disrupt mucosal homeostasis and are closely related to IBD in humans and colitis in mice.Therefore,preventing or correcting the imbalance of microbiota may serve as a novel prevention or treatment strategy for IBD.We review the most recent evidence for direct or indirect interventions targeting intestinal microbiota for treatment of IBD in order to overcome the current limitations of IBD therapies and shed light on personalized treatment options. | Bei Yue Zhi-Lun Yu Cheng Lv Xiao-Long Geng Zheng-Tao Wang Wei Dou | 2020 | World Journal of Gastroenterology2020，26，30： | 7 |
| 12 | Step-scheme CdS/TiO_(2) nanocomposite hollow microsphere with enhanced photocatalytic CO_(2) reduction activity显示文摘Converting solar energy into chemical energy by artificial photosynthesis is promising in addressing the issues of the greenhouse effect and fossil fuel crisis.Herein,a novel photocatalyst,i.e.CdS/TiO_(2) hollow microspheres(HS),were dedicatedly designed to boost overall photocatalytic efficiency.TiO_(2) nanoparticles were in-situ decorated on the inside and outside the shell of Cd S HS,ensuring close contact between TiO_(2) and CdS.The CdS/TiO2 HS with abundant mesopores inside of the shell boost the light absorption via multiscattering effect as well as accessible to reactions in all directions.The heterojunction was scrutinized and the charge transfer across it was revealed by in-situ irradiated X-ray photoelectron spectroscopy(ISI-XPS).Ultimately,the charge transfer in this composite was determined to follow stepscheme mechanism,which not only facilitates the separation of charge carriers but also preserves strong redox ability.Benefited from the intimate linkage between Cd S and TiO_(2) and the favorable step-scheme heterojunction,enhanced photocatalytic CO_(2) reduction activity was accomplished.The CH4 yield rate of CdS/TiO_(2) reaches 27.85μmol g^(–1) h^(–1),which is 145.6 and 3.8 times higher than those of pristine CdS and TiO_(2),respectively.This work presents a novel insight into constructing step-scheme photocatalytic system with desirable performance. | Zhongliao Wang Yifan Chen Liuyang Zhang Bei Cheng Jiaguo Yu Jiajie Fan | 2020 | Journal of Materials Science & Technology2020，56，21： | 7 |
| 13 | Tissue microarrays in pathological examination of apoptotic acinar cells induced by dexamethasone in the pancreas of rats with severe acute pancreatitis显示文摘BACKGROUND:The good therapeutic effects of large dose of dexamethasone on severe acute pancreatitis(SAP) patients have been proved.This study was designed to investigate the influence of dexamethasone on apoptosis of acinar cells in the pancreas of rats with SAP and the protein expression of the apoptosis-regulating genes Bax and Bcl-2. METHODS:Ninety Sprague-Dawley rats with SAP were randomly divided into a model group and a dexamethasone treated group(45 rats in each group),and another 45 rats formed the sham operation group.Survival rates were calculated and gross pathological changes in the pancreas of each group were observed under a light microscope 3,6 and 12 hours after operation.Tissue microarray technology was applied to prepare pancreatic tissue sections.The changes in Bax and Bcl-2 protein expression levels of pancreatic tissues from each group were assessed by immunohistochemical staining,and TUNEL staining was used to evaluate changes in apoptosis index. RESULTS:The model and treated groups did not differ in mortality at each time point.The pathological score for the pancreas in the treated group was significantly lower than that in the model group at 3 and 6 hours.The positive rates of Bax protein expression in the head and tail of the pancreas in the treated group at all time points were all markedly higher than those of the model group. The positive rate of Bcl-2 protein expression in the head of the pancreas in the treated group was significantly higher than that of the model group at 3 hours.TUNEL staining showed that the pancreas head and tail apoptosis indices of the treated group were markedly higher than those of the model group after 6 hours. CONCLUSIONS:Apoptosis may be a protective response to pancreatic cell injury.The mechanism of action of dexamethasone in treating SAP may be related to the apoptosis of acinar cells in the pancreas induced by apoptosis-regulating genes such as Bax and Bcl-2. The advantages of tissue microarrays in pathological examination of the pancreas include saving of time and energy,efficiency and highly representative. | Zhang, Xi-Ping Tian, Hua Lu, Bei Chen, Li Xu, Ru-Jun Wang, Ke-Yi Wang, Zhi-Wei Cheng, Qi-Hui Shen, Hai-Ping | 2007 | Hepatobiliary & Pancreatic Diseases International2007，6，5： | 7 |
| 14 | Observation and modeling on irregular purple soil water infiltration process显示文摘The infiltration process is a critical link between surface water and groundwater. In this research, a specific device to observe infiltration processes in homogeneous and heterogeneous soils with triangular and inverted triangular profiles was designed, and the Green-Ampt model was employed for the process simulation. The results indicate that(1) the wetting front in coarse texture soils transports faster than in fine texture soils;(2) for the homogeneous case, the wetting front in triangularshaped soils transports faster than the inverted triangular type, but the triangular-shaped soils show a lower infiltration rate;(3) in the initial step, the wetting front in triangular-shaped soils shows higher transport speed, but depicts lower speed with increase in the time;(4) both the wetting front and infiltration rate show a significant exponential relation with the time. From these findings, an empirical model was developed which agrees well with the observed data and provides a useful method for this field of soil research. | CHENG Dong-bing DONG Lin-yao QIAN Feng SUN Bei | 2017 | Journal of Mountain Science2017，14，6： | 6 |
| 15 | Rapid communication Preparation of TiO_2 nanometer thin films with high photocatalytic activity by reverse micellar method显示文摘Two kinds of TiO2 nanometer thin films were prepared on stainless steel by the reverse micellar and sol-gelmethods, respectively. The calcined TiO2 thin films were characterized by X-ray diffraction (XRD), atomic force micros-copy (AFM), BET surface area and X-ray photoelectron spectroscopy (XPS). Photocatalytic activity was evaluated byphotocatalytic decoloration of methyl orange aqueous solution. The results showed that the TiO2 thin films prepared by re-verse micellar method (designated as RM-TiO2 films) showed higher photocatalytic activity than those by sol-gel method(designated as SG-TiO2 films). This is attributed to the fact that the former is composed of smaller monodispersed sphericalparticles with a size of about 15 nm and possesses higher surface areas. | WU Qide, CHENG Bei, and ZHANG GaokeThe Key Laboratory of Silicon Materials Science and Engineering of Ministry of Education, Wuhan University of Technology, Wuhan 430070, China (Received 2003-01-10) | 2003 | Rare Metals2003，22，2： | 6 |
| 16 | Research of multilayers in EUV, soft X-ray and X-ray显示文摘To develop beam splitters for soft X-ray laser Michelson interferometer at 13.9 nm, Mo/Si multilayers of 100 nm thickness deposited on both sides of silicon nitride were fabricated by using DC magnetron sputtering. Initial evaluation of their reflectivity and transmission showed that reflectivity and transmission were above 10% and 25%. The broadband analyzers have been designed, fabricated and characterized for 13~20 nm polarization measurements. The measured results are in good agreement with the design. The supermirrors with different angular intervals at 0.154 nm have been designed, fabricated and characterized. | WANG Zhan-shan WANG Feng-li ZHANG Zhong WANG Hong-chang WU Wen-juan ZHANG Shu-min XU Yao GU Zhong-xiang CHENG Xin-bin L1 Cun-xia WU Yong-rong WANG Bei QIN Shu-jin CHEN Ling-yan | 2005 | 光学精密工程2005，13，4： | 6 |
| 17 | Analysis of trace mercury in water by solid phase extraction using dithizone modified nanometer titanium dioxide and cold vapor atomic absorption spectrometry显示文摘A new method for analysis of trace mercury in water samples was developed, based on the combination of preconcentration/separation using dithizone-modified nanometer titanium dioxide (TiO2) as a solid phase extractant and determination by cold vapor atomic adsorption spectrometry (CVAAS). Dithizone was dissolved with alcohol and loaded on the surface of nano-sized TiO2 powders by stirring. The static adsorption behavior of Hg2+on the dithizone-modified nanoparticles was investigated in detail. It was found that excellent adsorption ratio for Hg2+ could be obtained in the pH range of 7-8 with an oscillation time of 15 min, and a 5 mL of 3.5 molL1 HCl solution could quantitatively elute Hg2+ from nanometer TiO2 powder. Common coexisting ions caused no obvious influence on the determination of mercury. The mechanisms for the adsorption and desorption were discussed. The detection limit (3σ) for Hg2+ was calculated to be 5 ngL1. The proposed method was applied to the determination of Hg2+ in a mineral water sample and a Zhujiang River water sample. By the standard addition method, the average recoveries were found to be 94.4%-108.3% with RSD (n = 5) of 2.9%-3.5%. | MA Xiaoguo HUANG Bei CHENG Meiqing | 2007 | Rare Metals2007，26，6： | 5 |
| 18 | Expressing p20 hairpin RNA of Citrus tristeza virus confers Citrus aurantium with tolerance/resistance against stem pitting and seedling yellow CTV strains显示文摘The Citrus tristeza virus(CTV) uses 3 silencing suppressor genes, p20, p23 and p25, to resist the attacks from its Citrus hosts. Inactivating these genes is therefore obviously a potential defensive option in addition to the current control strategies including aphid management and the use of mild strain cross protection. In this study, we cloned partial DNA fragments from the three genes, and used them to construct vectors for expressing hairpin RNAs(hp RNAs). To facilitate the formation of hp RNAs, the constructs were introduced in a loop structure. Following transformation of sour orange(Citrus aurantium) with these constructs, 8 p20 hp RNA(hp20) and 1 p25 hp RNA(hp25) expressing lines were obtained. The 7 hp20 transgenic lines were further characterized. Their reactions to CTV were tested following inoculation with CT14 A and/or TR-L514, both of which are severe strains. Results showed that 3 lines(hp20-5, hp20-6 and hp20-8) were completely resistant to TR-L514 under greenhouse conditions for no detectable viral load was found in their leaves by PCR. However, they exhibited only partial suppression of TR-L514 under screen house conditions since the virus was detected in their leaves, though 2 months later compared to non-transgenic controls. Further tests showed that hp20-5 was tolerant also to CT14 A under screen house conditions. The growth of hp20-5 was much better than others including the controls that were concurrently challenged with CT14 A. These results showed that expressing p20 hp RNA was sufficient to c onfer sour orange with CTV resistance/tolerance. | CHENG Chun-zhen YANG Jia-wei YAN Hu-bin BEI Xue-jun ZHANG Yong-yan LU Zhi-ming ZHONG Guang-yan | 2015 | Journal of Integrative Agriculture2015，14，9： | 5 |
| 19 | Prognostic value of lymphovascular invasion in Bismuth-Corlette type Ⅳ hilar cholangiocarcinoma显示文摘AIM To assess the prognostic value of lymphovascular invasion(LVI)in Bismuth-Corlette typeⅣhilar cholangiocarcinoma(HC)patients. METHODS A retrospective analysis was performed on 142consecutively recruited typeⅣHC patients undergoing radical resection with at least 5 years of followup.Survival analysis was performed by the KaplanMeier method,and the association between the clinicopathologic variables and survival was evaluated by log-rank test.Multivariate analysis was adopted to identify the independent prognostic factors for overall survival(OS)and disease-free survival(DFS).Multiple logistic regression analysis was performed to determine the association between LVI and potential variables. RESULTS LVI was confirmed histopathologically in 29(20.4%)patients.Multivariate analysis showed that positive resection margin(HR=6.255,95%CI:3.485-11.229,P<0.001),N1 stage(HR=2.902,95%CI:1.132-7.439,P=0.027),tumor size>30 mm(HR=1.942,95%CI:1.176-3.209,P=0.010)and LVI positivity(HR=2.799,95%CI:1.588-4.935,P<0.001)were adverse prognostic factors for DFS.The independent risk factors for OS were positive resection margin(HR=6.776,95%CI:3.988-11.479,P<0.001),N1 stage(HR=2.827,95%CI:1.243-6.429,P=0.013),tumor size>30 mm(HR=1.739,95%CI:1.101-2.745,P=0.018)and LVI positivity(HR=2.908,95%CI:1.712-4.938,P<0.001).LVI was associated with N1 stage and tumor size>30 mm.Multiple logistic regression analysis indicated that N1 stage(HR=3.312,95%CI:1.338-8.198,P=0.026)and tumor size>30 mm(HR=3.258,95%CI:1.288-8.236,P=0.013)were associated with LVI. CONCLUSION LVI is associated with N1 stage and tumor size>30mm and adversely influences DFS and OS in typeⅣHC patients. | Bei Li Xian-Ze Xiong Yong Zhou Si-Jia Wu Zhen You Jiong Lu Nan-Sheng Cheng | 2017 | World Journal of Gastroenterology2017，23，36： | 5 |
| 20 | 人乳头瘤病毒16/18分型在细胞学轻度异常的低度宫颈病变中的预测价值(英文)显示文摘目的:评估人乳头瘤病毒(HPV)16/18分型在细胞学正常或轻度异常的低度宫颈病变中的预测价值,为临床分流决策提供依据。创新点:目前对于细胞学正常或轻度异常的低度宫颈病变患者缺乏有效分流策略。本研究首次探索HPV16/18分型对这部分患者疾病转归的预测价值。方法:新招募细胞学正常或轻度异常的低度宫颈病变患者,以6个月为间隔随访2年,采用Cox回归模型对入组HPV16/18分型结果与疾病转归进行关联分析。结论:对于HPV16/18阳性,细胞学轻度异常,且30岁及以上的低度宫颈病变患者,可以考虑比保守随访更积极的诊疗方案。 | Jing YE Bei CHENG Yi-fan CHENG Ye-li YAO Xing XIE Wei-guo LU Xiao-dong CHENG | 2017 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2017，18，3： | 5 |
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