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| 1 | CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes显示文摘染色体编辑工具例如定期聚类短 palindromic 重复(CRISPR ) 联系了的 interspaced 系统(Cas ) 广泛地被用来包括动物接合子和人的房间在模型系统修改基因,并且为基本研究和临床的应用保持巨大的诺言。迄今为止,严肃的知识差距在人的早胚胎,并且在效率和在人的培植前胚胎使用象 CRISPR/Cas9 那样的技术的潜在的离开目标效果留在我们 DNA 修理机制的理解。在这份报告,我们使用了推进的接合子调查 CRISPR/Cas9-mediated 基因在人的房间编辑的 tripronuclear (3PN ) 。我们发现 CRISPR/Cas9 能有效地劈开内长的 -globin 基因(HBB ) 。然而,相应再结合的效率指导了 HBB 的修理(HDR ) 是低的,编辑胚胎是马赛克。离开目标劈开在由 T7E1 试金并且 whole-exome 定序揭示了的这些 3PN 接合子也是明显的。而且,内长的 delta-globin 基因(HBD ) 对 HBB 相应,与外长的施主 oligos 竞争了充当修理模板,导致倔强的变化。我们的数据也显示在这些胚胎的 HBB 地点的修理通过非转线路 HDR 小径优先地发生了。一起拿,我们的工作加亮紧迫的需要进一步改进 CRISPR/Cas9 平台的忠实和特性,为编辑的 CRSIPR/Cas9-mediated 的任何临床的应用程序的一个前提。 | Puping Liang Yanwen Xu Xiya Zhang Chenhui Ding Rui Huang Zhen Zhang Jie Lv Xiaowei Xie Yuxi Chen Yujing Li Ying Sun Yaofu Bai Zhou Songyang Wenbin Ma Canquan Zhou Junjiu Huang | 2015 | Protein & Cell2015，6，5： | 149 |
| 2 | NK cell-based immunotherapy for malignant diseases显示文摘生来的杀手(NK ) 房间对癌症在主人免疫起关键作用。在反应,癌症开发机制逃离 NK 房间攻击或导致有缺点的 NK 房间。当前的 NK 基于房间的癌症免疫疗法试图用几条途径克服 NK 房间麻痹。一条途径使用扩展 allogeneic NK 房间,它没被象自体同源的 NK 房间一样的自我 histocompatibility 抗原禁止,为采纳细胞的免疫疗法。另一条采纳转移途径使用稳定的 allogeneic NK 房间线,它为质量控制和大规模生产是更实际的。第三条途径是新鲜 NK 房间或 NK 房间线的基因修正高度表示 cytokines, Fc 受体或妄想的肿瘤抗原受体。治疗学的 NK 细胞能从各种各样的来源被导出,包括细胞,干细胞或甚至导致的 pluripotent 干细胞( iPSCs ),和许多激发器能被用于的外设或绳索血在实验室或好生产实践( GMP )的大规模生产设备包括可溶的生长因素,使不能调动的分子或抗体,和另外的细胞的使活跃之物。到在临床的试用的癌症的对待几类型的 NK 房间治疗的一张表这里被考察。基于 NK 的免疫疗法的几条不同途径例如织物特定的 NK 房间,漂亮面向受体的 NK 房间和化学上对待的 NK 房间,被讨论。一些新技术或策略到由非侵略的成像的监视器 NK 房间治疗,预定 NK 房间治疗由的效率在 vivo 实验并且在临床的试用评估 NK 房间治疗途径也被介绍。 | Min Cheng Yongyan Chen Weihua Xiao Rui Sun Zhigang Tian | 2013 | Cellular & Molecular Immunology2013，10，3： | 61 |
| 3 | Pathogenic T-cells and inflammatory monocytes incite inflammatory storms in severe COVID-19 patients显示文摘Pathogenic human coronavirus infections,such as severe acute respiratory syndrome CoV(SARS-CoV)and Middle East respiratory syndrome CoV(MERS-CoV),cause high morbidity and mortality[1,2].Recently,a severe pneumonia-associated respiratory syndrome caused by a new coronavirus(SARS-CoV-2)was reported in December 2019 in the city of Wuhan,Hubei province,China[3-5],which was also named as pneumonia-associated respiratory syndrome(PARS)[6]and can cause coronavirus disease 2019(COVID-19)to seriously endanger human health. | Yonggang Zhou Binqing Fu Xiaohu Zheng Dongsheng Wang Changcheng Zhao Yingjie Qi Rui Sun Zhigang Tian Xiaoling Xu Haiming Wei | 2020 | National Science Review2020，7，6： | 54 |
| 4 | Changes of net primary productivity in China during recent 11 years detected using an ecological model driven by MODIS data显示文摘网主要生产率(NPP ) 是陆上的碳周期的一个重要部件。精确地在中国印射 NPP 的空间时间的变化为全球碳骑车是关键的学习。在这研究,基于过程的北方的生态系统生产率模拟器(BEPS ) 被采用从 2000 ~ 2010 为时期在中国的生态系统学习 NPP 的变化。BEPS 模型首先用在在不同区域测量的典型流动地点和森林 NPP 测量的粗野主要生产率(GPP ) 被验证。然后,它与叶区域索引( LAI )被驾驶从中等分辨率成像分光辐射函数( MODIS )反射和陆地盖子产品和气象学的数据的 inversed 在 753 个国家基本气象学的车站从观察插入内推从1月1日在每日的时间步和 500 m 的空间分辨率模仿 NPP , 2000 到2010年12月31日。确认证明 BEPS 能在中国的不同区域捕获基于塔的 GPP 的季节的变化和森林 NPP 的空间可变性。年度 NPP 的估计的国家总数从 2.63 变化了到 2.84Pg C 獥吗? | Yibo LIU Weimin JU Honglin HE Shaoqiang WANG Rui SUN Yuandong ZHANG | 2013 | Frontiers of Earth Science2013，7，1： | 30 |
| 5 | Protective effect of glutamine on intestinal injury and bacterial community in rats exposed to hypobaric hypoxia environment显示文摘AIM:To investigate the protective effect of glutamine(Gln)on intestinal injury and the bacterial community in rats exposed to hypobaric hypoxia environment.METHODS:Sprague-Dawley rats were divided into control,hypobaric hypoxia(HH),and hypobaric hypoxia+Gln(5.0 g/kg BW·d)(HG)groups.On the first 3 d,all rats were placed in a normal environment.After the third day,the HH and HG groups were transferred into a hypobaric chamber at a simulated elevation of 7000m for 5 d.The rats in the HG group were given Gln by gavage daily for 8 d.The rats in the control and HH groups were treated with the same volume of saline.The intestinal morphology,serum levels of malondialdehyde(MDA),superoxide dismutase(SOD),interleukin-6 (IL-6),tumor necrosis factor-α(TNF-α),interferon-gamma(IFN-γ)and diamino oxidase(DAO)were examined.We also evaluated the expression levels of occludin,toll-like receptor 4(TLR4),nuclear factor-κB p65(NF-κB p65)and myeloid differentiation factor 88(MyD88),and examined the bacterial community in caecal contents.RESULTS:Hypobaric hypoxia induced the enlargement of the heart,liver,lung and kidney,and caused spleen atrophy.Intestinal villi damage was also observed in the HH group.Supplementation with Gln significantly alleviated hypobaric-induced damage to main organs including the intestine,increased serum SOD(1.14±0.03 vs 0.88±0.04,P<0.05)and MDA(8.35±1.60,P<0.01)levels and decreased serum IL-6(1172.13±30.49 vs 1407.05±34.36,P<0.05),TNF-α(77.46±0.78 vs 123.70±3.03,P<0.001),IFN-γ(1355.42±72.80 vs 1830.16±42.07,P<0.01)and DAO(629.30±9.15 vs 524.10±13.34,P<0.001)levels.Moreover,Gln significantly increased occludin(0.72±0.05 vs 0.09±0.01,P<0.001),TLR4(0.15±0.05 vs 0.30±0.09,P<0.05),MyD88(0.32±0.08 vs 0.71±0.06,P<0.01),and NF-κB p65(0.16±0.04 vs 0.44±0.03,P<0.01)expression levels and improved the intestinal bacterial community.CONCLUSION:Gln treatment protects from intestinal injury and regulates the gut flora imbalance in hypoxia environment.These effects may be related to the TLR4/MyD88/NF-κB signaling pathway. | Chun-Lan Xu Rui Sun Xiang-Jin Qiao Cui-Cui Xu Xiao-Ya Shang Wei-Ning Niu | 2014 | World Journal of Gastroenterology2014，20，16： | 29 |
| 6 | Multiple methods of surgical treatment combined with primary IOL implantation on traumatic lens subluxation/dislocation in patients with secondary glaucoma显示文摘AIM:To describe clinical findings and complications from cases of traumatic lens subluxation/dislocation in patients with secondary glaucoma,and discuss the multiple treating methods of operation combined with primary intraocular lens(IOL)implantation.METHODS:Non-comparativeretrospectiveobservational case series.Participants:30 cases(30 eyes)of lens subluxation/dislocation in patients with secondary glaucoma were investigated which accepted the surgical treatment by author in the Ophthalmology of Xi'an No.4Hospital from 2007 to 2011.According to the different situations of lens subluxation/dislocation,various surgical procedures were performed such as crystalline lens phacoemulsification,crystalline lens phacoemulsification combined anterior vitrectomy,intracapsular cataract extraction combined anterior vitrectomy,lensectomy combined anterior vitrectomy though peripheral transparent cornea incision,pars plana lensectomy combined pars plana vitrectomy,and intravitreal cavity crystalline lens phacofragmentation combined pars plana vitrectomy.And whether to implement trabeculectomy depended on the different situations of secondary glaucoma.The posterior chamber intraocular lenses(PC-IOLs)were implanted in the capsular-bag or trassclerally sutured in the sulus decided by whether the capsular were present.Main outcome measures:visual acuity,intraocular pressure,the situation of intraocular lens and complications after the operations.RESULTS:The follow-up time was 11-36mo(21.4±7.13).Postoperative visual acuity of all eyes were improved;28 cases maintained IOP below 21 mm Hg;2cases had slightly IOL subluxation,4 cases had slightlytilted lens optical area;1 case had postoperative choroidal detachment;4 cases had postoperative corneal edema more than 1wk,but eventually recovered transparent;2 cases had mild postoperative vitreous hemorrhage,and absorbed 4wk later.There was no postoperative retinal detachment,IOL dislocation,and endophthalmitis.CONCLUSION:To take early treatment of traumatic lens subluxation/dislocation in patients with secondary glaucoma by individual surgical plan based on the different eye conditions would be safe and effective,which can effectively control the intraocular pressure and restore some vision. | Rui Wang Chun-Chao Bi Chun-Ling Lei Wen-Tao Sun Shan-Shan Wang Xiao-Juan Dong | 2014 | International Journal of Ophthalmology(English edition)2014，7，2： | 26 |
| 7 | Long-Term Effect of No-Tillage on Soil Organic Carbon Fractions in a Continuous Maize Cropping System of Northeast China显示文摘Increasing evidence has shown that conservation tillage is an effective agricultural practice to increase carbon (C) sequestration in soils. In order to understand the mechanisms underlying the responses of soil organic carbon (SOC) to tillage regimes, physical fractionation techniques were employed to evaluate the effect of long-term no-tillage (NT) on soil aggregation and SOC fractions. Results showed that NT increased the concentration of total SOC by 18.1% compared with conventional tillage (CT) under a long-term maize (Zea mays L.) cropping system in Northeast China. The proportion of soil large macroaggregates (> 2000 μm) was higher in NT than that in CT, while small macroaggregates (250-2000 μm) showed an opposite trend. Therefore, the total proportion of macroaggregates (> 2000 and 250-2000 μm) was not affected by tillage management. However, C concentrations of macroaggregates on a whole soil basis were higher under NT relative to CT, indicating that both the amount of aggregation and aggregate turnover affected C stabilization. Carbon concentrations of intra-aggregate particulate organic matter associated with microaggregates (iPOM m) and microaggregates occluded within macroaggregates (iPOM mM) in NT were 1.6 and 1.8 times greater than those in CT, respectively. Carbon proportions of iPOM m and iPOM mM in the total SOC increased from 5.4% and 6.3% in CT to 7.2% and 9.7% in NT, respectively. Furthermore, the difference in the microaggregate protected C (i.e., iPOM m and iPOM mM) between NT and CT could explain 45.4% of the difference in the whole SOC. The above results indicate that NT stimulates C accumulation within microaggregates which then are further acted upon in the soil to form macroaggregates. The shift of SOC within microaggregates is beneficial for long-term C sequestration in soil. We also corroborate that the microaggregate protected C is useful as a pool for assessing the impact of tillage management on SOC storage. | HUANG Shan SUN Yan-Ni RUI Wen-Yi LIU Wu-Ren ZHANG Wei-Jian | 2010 | Pedosphere2010，20，3： | 26 |
| 8 | Effect and Safety of Guanxinning Tablet(冠心宁片) for Stable Angina Pectoris Patients with Xin(Heart)-Blood Stagnation Syndrome:A Randomized,Multicenter,Placebo-Controlled Trial显示文摘Objective: To investigate the effect and safety of Guanxinning Tablet(冠心宁片, GXN) for the treatment of stable angina pectoris patients with Xin(Heart)-blood stagnation syndrome(XBSS). Methods: One hundred and sixty stable angina pectoris patients with XBSS were randomly assigned to receive GXN(80 cases) or placebo(80 cases, Guanxinning simulation tablets, mainly composed of lactose), 4 tablets(0.38 g/tablet), thrice daily for 12 weeks. After treatment, an exercise stress test(treadmill protocol), Chinese medicine(CM) syndrome score, electrocardiogram(ECG), and nitroglycerin withdrawal rate were evaluated and compared in the patients between the two groups. Meanwhile, adverse events(AEs) were evaluated during the whole clinical trial. Results: Compared with the control group, the time extension of exercise duration in the GXN group increased 29.28±17.67 s after treatment(P>0.05);moreover, the change of exercise duration in the GXN group increased 63.10±96.96 s in subgroup analysis(P<0.05). The effective rates of angina pectoris, CM syndrome and ECG as well as nitroglycerin withdrawal rate were 81.33%, 90.67%, 45.76%, and 70.73%, respectively in the GXN group, which were all significantly higher than those in the control group(40.58%, 75.36%, 26.92%, 28.21%, respectively, P<0.05). Conclusion: GXN was a safe and effective treatment for stable angina pectoris patients with XBSS at a dose of 4 tablets, thrice daily. | SUN Ming-yue MIAO Yang JIN Min DONG Yao-rong LIU Shu-rong WANG Mu-lan GAO Rui | 2019 | Chinese Journal of Integrative Medicine2019，25，9： | 22 |
| 9 | Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity显示文摘感觉神经原被不同发信号的网络和接受特征区分。因此,感觉神经原类型能被连接基于 transcriptome 的神经原与感觉显型打字定义。这里,我们分类老鼠的 somatosensory 神经原由高范围的单个房间的定序 RNA 的背面的根中心(DRG )(10 950 ±1 218 基因每神经原) 并且神经原基于尺寸的层次聚类。而且,对皮肤的刺激作出回应的单个 DRG 神经原被记录用一在里面 vivo 整个房间的补丁夹钳技术并且由神经原类型分类基因标记。小直径 DRG 神经原被分类进低阀值的 mechanoreceptor 的一种类型和 mechanoheat nociceptors (MHN ) 的五种类型。每 MHN 类型进一步被分成二种子类型。大 DRG 神经原被分成四种类型,包括表示联系 BAI1 的蛋白质 2-like 的 neurexophilin 1 快车 MHN 和机械 nociceptors (MN ) 1 (Baiap2l1 ) 。表示 trafficking 蛋白质粒子建筑群 3-like 和 Baiap2l1 显著的 MN 的 Mechanoreceptors 各被细分进二种子类型。这些结果为编目 somatosensory 神经原和他们的 transcriptome 数据库提供一个新系统。 | Chang-Lin Li Kai-Cheng Li Dan Wu Yan Chen Hao Luo Jing-Rong Zhao Sa-Shuang Wang Ming-Ming Sun Ying-Jin Lu Yan-Qing Zhong Xu-Ye Hu Rui Hou Bei-Bei Zhou Lan Bao Hua-Sheng Xiao Xu Zhang | 2016 | Cell Research2016，26，1： | 21 |
| 10 | The ChinaMAP analytics of deep whole genome sequences in 10,588 individuals显示文摘Metabolic diseases are the most common and rapidly growing health issues worldwide.The massive population-based human genetics is crucial for the precise prevention and intervention of metabolic disorders.The China Metabolic Analytics Project(ChinaMAP)is based on cohort studies across diverse regions and ethnic groups with metabolic phenotypic data in China.Here,we describe the centralized analysis of the deep whole genome sequencing data and the genetic bases of metabolic traits in 10,588 individuals from the ChinaMAP.The frequency spectrum of variants,population structure,pathogenic variants and novel genomic characteristics were analyzed.The individual genetic evaluations of Mendelian diseases,nutrition and drug metabolism,and traits of blood glucose and BMI were integrated.Our study establishes a large-scale and deep resource for the genetics of East Asians and provides opportunities for novel genetic discoveries of metabolic characteristics and disorders. | Yanan Cao Lin Li Min Xu Zhimin Feng Xiaohui Sun Jieli Lu Yu Xu Peina Du Tiange Wang Ruying Hu Zhen Ye Lixin Shi Xulei Tang Li Yan Zhengnan Gao Gang Chen Yinfei Zhang Lulu Chen Guang Ning Yufang Bi Weiqing Wang The ChinaMAP Consortium | 2020 | Cell Research2020，30，9： | 20 |
| 11 | Fluxes of CH4 and N_2O from soil under a tropical seasonal rain forest in Xishuangbanna, Southwest China显示文摘从在在 Xishuangbanna 的热带季节的雨林下面的土壤的 CH4 和 N2O 流动,西南中国用关上的静态的房间技术和气体色层法方法被测量一年。三个处理被放在学习的地里:(A) 没有崽,有垃圾的(B) ,和有垃圾和幼苗的(C) 。结果证明在我们的学习的土壤是大气的 CH4 的一个水池和大气的 N2O 的来源。从处理 A, B,和 C 的观察吝啬的 CH4 流动是 50.0 ± 4.0, 35.9 ± 2.8, 31.6 ± 2.8 μ g C/(m2 · h ) ,分别地并且在 2003 的计算年度流动分别地是 4.1, 3.1,和 2.9 kgC/hm2。从处理 A, B,和 C 的观察吝啬的 N2O 流动是 30.9 ± 3.1, 28.2 ± 3.5, 50.2 ± 3 .7 μ g N/(m2 · h ) ,分别地并且在 2003 的计算年度流动分别地是 4.1, 3.1,和 2.9 kgC/hm2。从处理 A, B,和 C 的观察吝啬的 N2O 流动是 30.9 ± 3.1, 28.2 ± 3.5, 50.2 ± 3 .7 μ g N/(m2 · h ) ,分别地并且在 2003 的计算年度流动是 2.8, 2.6,和 3.7 kgN/hm 2 分别地。在 CH4 和 N2O 流动的季节变化在所有三个处理之中是重要的。垃圾的存在减少了 CH4 举起在湿季节期间(P < 0.05 ) ,然而并非在干旱期期间。调停幼苗的 N2O 排出物有类似的增加在期间湿并且干旱期,显示幼苗在两个季节增加了 N2O 排放。一种强壮的积极关系为处理 B 和处理 C 为所有三个处理,和在 CH4 流动和土壤温度之间的弱关系在 CH4 流动和土壤潮湿之间存在。N2O 流动为所有三个处理与土壤温度相关。 | YAN Yuping SHA Liqing CAO Min ZHENG Zheng TANG Jianwei WANG Yinghong ZHANG Yiping WANG Rui LIU Guangren WANG Yuesi SUN Yang | 2008 | Journal of Environmental Sciences2008，20，2： | 18 |
| 12 | p53 upregulated by HIF-la promotes hypoxiainduced G2/M arrest and renal fibrosis in vitro and in vivo显示文摘Hypoxia plays an important role in the genesis and progression of renal fibrosis.The underlying mechanisms, however, have not been sufficiently elucidated. We examined the role of p53 in hypoxia-induced renal fibrosis in cell culture (human and rat renal tubular epithelial cells) and a mouse unilateral ureteral obstruction (UUO) model. Cell cycle of tubular cells was determined by flow cytometry, and the expression of profibrogenic factors was determined by RT-PCR, immunohistochemistry, and western blotting. Chromatin immunoprecipitation and luciferase reporter experiments were performed to explore the effect of HIF-lα on p53 expression. We showed that, in hypoxic tubular cells, p53 upregulation suppressed the expression of CDK1 and cyclins Bl and DI, leading to cell cycle (G2/M) arrest (or delay) and higher expression of TGF-β, CTGF, collagens, and fibronectin. p53 suppression by siRNA or by a specific p53 inhibitor (PIF-α) triggered opposite effects preventing the G2/M arrest and profibrotic changes. In vivo experiments in the UUO model revealed similar antifibrotic results following intraperitoneal administration of PIF-α(2.2 mg/kg). Using gain-of-function, loss-of-function, and luciferase assays, we further identified an HRE3 region on the p53 promoter as the HIF-lα-binding site. The HIF-la-HRE3 binding resulted in a sharp transcriptional activation of p53. Collectively, we show the presence of a hypoxia-activated, p53-responsive profibrogenic pathway in the kidney. During hypoxia, p53 upregulation induced by HIF-la suppresses cell cycle progression, leading to the accumulation of G2/M cells, and activates profibrotic TGF-β and CTGF-mediated signaling pathways, causing extracellular matrix production and renal fibrosis. | Limin Liu Peng Zhang Ming Bai Lijie He Lei Zhang Ting Liu Zhen Yang Menglu Duan Minna Liu Baojian Liu Rui Du Qi Qian Shiren Sun | 2019 | Journal of Molecular Cell Biology2019，11，5： | 18 |
| 13 | Survival of transplanted neurotrophin-3 expressing human neural stem cells and motor function in a rat model of spinal cord injury显示文摘<正>BACKGROUND:Many methods have been attempted to repair nerves following spinal cord injury, including peripheral nerve transplantation,Schwann cell transplantation,olfactory ensheathing cell transplantation,and embryonic neural tissue transplantation.However,there is a need for improved outcomes. OBJECTIVE:To investigate the repair feasibility for rat spinal cord injury using human neural stem cells(hNSCs) genetically modified by lentivirus to express neurotrophin-3. DESIGN,TIME AND SETTING:In vitro cell biological experiment and in vivo randomized,controlled, genetic engineering experiment were performed at the Third Military Medical University of Chinese PLA and First People's Hospital of Yibin,China from March 2006 to December 2007. MATERIALS:A total of 64 adult,female,Wistar rats were used for the in vivo study.Of them,48 rats were used to establish models of spinal cord hemisection,and were subsequently equally and randomly assigned to model,genetically modified hNSC,and normal hNSC groups.The remaining 16 rats served as normal controls. METHODS:hNSCs were in vitro genetically modified by lentivirus to secrete both green fluorescence protein and neurotrophin-3.Neurotrophin-3 expression was measured by Western blot. Genetically modified hNSC or normal hNSC suspension(5×10~5) was injected into the rat spinal cord following T_(10) spinal cord hemisection.A total of 5μL Dulbecco's-modified Eagle's medium was infused into the rat spinal cord in the model group.Transgene expression and survival of transplanted hNSCs were determined by immunohistochemistry.Motor function was evaluated using the Basso,Beattie,and Bresnahan(BBB) scale. MAIN OUTCOME MEASURES:The following parameters were measured:expression of neurotrophin-3 produced by genetically modified hNSCs,transgene expression and survival of hNSCs in rats,motor function in rats. RESULTS:hNSCs were successfully genetically modified by lentivirus to stably express neurotrophin-3.The transplanted hNSCs primarily gathered at,or around,the injection site two weeks following transplantation,and gradually migrated towards the surrounding tissue. Transplanted hNSCs were observed 7.0-8.0 mm away from the injection site.In addition,hNSCs were observed 10 weeks after transplantation.At week 4,BBB locomotor scores were significantly greater in the genetically modified hNSC and normal hNSC groups,compared with the model group (P<0.05),and scores were significantly greater in the genetically modified hNSC group compared with the normal hNSC group(P<0.05). CONCLUSION:hNSCs were genetically modified with lentivirus to stably secrete neurotrophin-3. hNSCs improved motor function recovery in rats following spinal cord injury. | Peiqiang Cai Guangyun Sun Peishu Cai Martin Oudega Rui Xiao Xuewen Wang Wei Li Yunbing Shu Cheng Cai Haihao Yang Xuebing Shan Wuhua Luo | 2009 | Neural Regeneration Research2009，4，7： | 18 |
| 14 | Transurethral seminal vesiculoscopy for recurrent hemospermia: experience from 419 cases显示文摘 | Rui Chen Lei Wang Xia Sheng Shu-Guang Piao Xin-Wen Nian Xin Cheng Tie Zhou Hui-Zhen Li Ya-Wei Liu Guang-Hua Chen Chun-Lei Zhang De-Pei Kong Guang-An Xiao Xin Lu Zhen-Yu Jia Zhi-Yong Liu Ying-Hao Sun | 2018 | Asian Journal of Andrology2018，20，5： | 18 |
| 15 | Minimally invasive surgery alone compared with intensity-modulated radiotherapy for primary stage I nasopharyngeal carcinoma显示文摘Background:The National Comprehensive Cancer Network guidelines recommend intensity-modulated radiotherapy(IMRT)as the primary curative treatment for newly diagnosed nasopharyngeal carcinoma(NPC),but the radiation-related complications and relatively high medical costs remain a consequential burden for the patients.Endoscopic nasopharyngectomy(ENPG)was successfully applied in recurrent NPC with radiation free and relatively low medical costs.In this study,we examined whether ENPG could be an effective treatment for localized stage I NPC.Methods:Ten newly diagnosed localized stage I NPC patients voluntarily received ENPG alone from June 2007 to September 2017 in Sun Yat-sen University Cancer Center.Simultaneously,the data of 329 stage I NPC patients treated with IMRT were collected and used as a reference cohort.The survival outcomes,quality of life(QOL),and medical costs between two groups were compared.Results:After a median follow-up of 59.0 months(95%CI 53.4-64.6),no death,locoregional recurrence,or distant metastasis was observed in the 10 patients treated with ENPG.The 5-year overall survival,local relapse-free survival,regional relapse-free survival,and distant metastasis-free survival among the ENPG-treated patients was similar to that among the IMRT-treated patients(100%vs.99.1%,100%vs.97.7%,100%vs.99.0%,100%vs.97.4%,respectively,P>0.05).In addition,compared with IMRT,ENPG was associated with decreased total medical costs($4090.42±1502.65 vs.$12620.88±4242.65,P<0.001)and improved QOL scores including dry mouth(3.3±10.5 vs.34.4±25.8,P<0.001)and sticky saliva(3.3±10.5 vs.32.6±23.3,P<0.001).Conclusions:ENPG alone was associated with promising long-term survival outcomes,low medical costs,and satisfactory QOL and might therefore be an alternative strategy for treating newly diagnosed localized stage I NPC patients who refused radiotherapy.However,the application of ENPG should be prudent,and prospective clinical tri-als were needed to further verify the results. | You-Ping Liu Xing Lv Xiong Zou Yi-Jun Hua Rui You Qi Yang Le Xia Shao-Yan Guo Wen Hu Meng-Xia Zhang Si-Yuan Chen Mei Lin Yu-Long Xie Li-Zhi Liu Rui Sun Pei-Yu Huang Wei Fan Xiang Guo Ming-Huang Hong Ming-Yuan Chen | 2019 | Cancer Communications2019，39，1： | 17 |
| 16 | Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway显示文摘Objective The aim of this study is to investigate whether microwave exposure would affect the N-methyl-D-aspartate receptor(NMDAR) signaling pathway to establish whether this plays a role in synaptic plasticity impairment. Methods 48 male Wistar rats were exposed to 30 m W/cm2 microwave for 10 min every other day for three times. Hippocampal structure was observed through H&E staining and transmission electron microscope. PC12 cells were exposed to 30 m W/cm2 microwave for 5 min and the synapse morphology was visualized with scanning electron microscope and atomic force microscope. The release of amino acid neurotransmitters and calcium influx were detected. The expressions of several key NMDAR signaling molecules were evaluated. Results Microwave exposure caused injury in rat hippocampal structure and PC12 cells, especially the structure and quantity of synapses. The ratio of glutamic acid and gamma-aminobutyric acid neurotransmitters was increased and the intracellular calcium level was elevated in PC12 cells. A significant change in NMDAR subunits(NR1, NR2 A, and NR2B) and related signaling molecules(Ca2+/calmodulin-dependent kinase II gamma and phosphorylated c AMP-response element binding protein) were examined. Conclusion 30 m W/cm2 microwave exposure resulted in alterations of synaptic structure, amino acid neurotransmitter release and calcium influx. NMDAR signaling molecules were closely associated with impaired synaptic plasticity. | XIONG Lu SUN Cheng Feng ZHANG Jing GAO Ya Bing WANG Li Feng ZUO Hong Yan WANG Shui Ming ZHOU Hong Mei XU Xin Ping DONG Ji YAO Bin Wei ZHAO Li PENG Rui Yun | 2015 | Biomedical and Environmental Sciences2015，28，1： | 16 |
| 17 | Prostate cancer research in China显示文摘在中国的前列腺癌症(PCa ) 研究在最近的年里的一个飞速上升的趋势上。在中国的第一染色体宽的协会研究(GWAS ) 鉴别二新 PCa 风险联系了单个核苷酸多型性(SNP ) 。下一代定序正在开始被过去常,产出新奇调查结果:基因熔化,长非编码的 RNA 和另外的变化。当各种各样的诊断 biomarkers 广泛地被调查了时, PCa 前进的机制被说明了。个性化的治疗基于基因因素, nano 药和繁体中文药是为 PCa 的试验性的治疗学的研究的焦点。这评论打算在中国在 PCa 研究在最近的进步之上打开灯并且以后指出可能的突破。 | Shan-Cheng Ren Rui Chen Ying-Hao Sun | 2013 | Asian Journal of Andrology2013，15，3： | 15 |
| 18 | Primary duct closure versus T-tube drainage after laparoscopic common bile duct exploration:a meta-analysis显示文摘Background and aims:Laparoscopic common bile duct exploration(LCBDE)is considered a safe and effective method for the removal of bile duct stones.However,the choice of primary duct closure(PDC)or T-tube drainage(TTD)technique after LCBDE is still controversial.This study aimed to compare the safety and effectiveness of PDC and TTD after LCBDE.Methods:Studies published before May 1,2021 in Pub Med,Web of Science,and Cochrane Library databases were searched to screen out randomized controlled trials(RCTs)and cohort studies to compare PDC with TTD.Meta-analyses of fixed effect and random effect models were performed using Rev Man 5.3.Results:A total of 1865 patients were enrolled in six RCTs and ten cohort studies.Regarding RCTs,the PDC group was significantly better than the TTD group in terms of operation time,total postoperative complications,postoperative hospital stay,and hospitalization expenses(all P<0.05).Based on cohort studies of the subgroup,the PDC group had shorter operation time,shorter postoperative hospital stay,less intraoperative blood loss,and limited total postoperative complications.Statistically,there were no significant differences in bile leakage,retained stones,stone recurrence,bile duct stricture,postoperative pancreatitis,other complications,or postoperative exhaust time between the TTD and PDC groups.Conclusions:Based on the available evidence,compared with TTD,PDC is safe and effective,and can be used as the first choice after transductal LCBDE in patients with choledocholithiasis. | Taifeng ZHU Haoming LIN Jian SUN Chao LIU Rui ZHANG | 2021 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2021，22，12： | 15 |
| 19 | Liquefaction macrophenomena in the great Wenchuan earthquake显示文摘On May 12,2008 at 14:28,a catastrophic magnitude Ms 8.0 earthquake struck the Sichuan Province of China.The epicenter was located at Wenchuan(31.00oN,103.40oE).Liquefaction macrophenomena and corresponding destruction was observed throughout a vast area of 500 km long and 200 km wide following the earthquake.This paper illustrates the geographic distribution of the liquefaction and the relationship between liquefaction behavior and seismic intensity,and summarizes the liquefaction macrophenomena,including sandboils and waterspouts,ground subsidence,ground fi ssures etc.,and relevant liquefaction features.A brief summary of the structural damage caused by liquefaction is presented and discussed.Based on comparisons with liquefaction phenomena observed in the 1976 Tangshan and 1975 Haicheng earthquakes,preliminary analyses were performed,which revealed some new features of liquefaction behavior and associated issues arising from this event.The site investigation indicated that the spatial non-uniformity of liquefaction distribution was obvious and most of the liquefi ed sites were located in regions of seismic intensity VIII.However,liquefaction phenomena at ten different sites in regions of seismic intensity VI were also observed for the fi rst time in China mainland.Sandboils and waterspouts ranged from centimeters to tens of meters,with most between 1 m to 3 m.Dramatically high water/sand ejections,e.g.,more than 10 m,were observed at four different sites.The sand ejections included silty sand,fi ne sand,medium sand,course sand and gravel,but the ejected sand amount was less than that in the 1976 Tangshan earthquake.Possible liquefaction of natural gravel soils was observed for the fi rst time in China mainland. | Chen Longwei Yuan Xiaoming Cao Zhenzhong Hou Longqing Sun Rui Dong Lin Wang Weiming Meng Fanchao Chen Hongjuan | 2009 | Earthquake Engineering and Engineering Vibration2009，8，2： | 14 |
| 20 | Significant value of 18F-FDG-PET/CT in diagnosing small cervical lymph node metastases in patients with nasopharyngeal carcinoma treated with intensity-modulated radiotherapy显示文摘Background: Little is known about the nature of metaistasis to small cervical lymph nodes(SCLNS) in the patients with nasopharyngeal carcinoma(NPC)examined by using 18-fluoro-2-deoxy-glucose(^(18)F-FDG) positron emission tomography/computed tomography(PET/CT).The present study aimed to evaluate the diagnostic values of PET/CT in identifying metastasis in SCLNs in NPC patients.Methods: Magnetic resonance images(MRI) and PET/CT scans for 470 patients with newly diagnosed, non-distant metastatic NPC were analyzed. Metastatic rates of SCLNs were defined by the positive number of SCLNs on PET/CT scans and total number of SCLNs on MRI scans. Receiver operating characteristic curve was applied to compare PET/CT-determined stage with MRI-determined stage.Results: In total, 2082 SCLNs were identified, with 808(38.8%) ≥ 5 and < 6 mm in diameter(group A), 526(25.3%)≥ 6 and < 7 mm in diameter(group B),374(18.0%)≥ 7 and < 8 mm in diameter(group C), 237(11.4%) ≥8 and<9 mm in diameter(group D),and 137(6.5%) ≥ 9 and <10 mm in diameter(group E).The overall metastatic rates examined by using PET/CT for groups A, B,C,D, and E were 3.5%, 8.0%, 31.3%, 60.0%, and 83.9%, respectively(P< 0.001). In level IV/Vb, the metastatic rate for nodes ≥ 8 mm was 84.6%. PET/CT examination resulted in modification of N category and overall stage for 135(28.7%) and 46(9.8%) patients, respectively. The areas under curve of MRIdetermined and PET/CT-determined overall stage were 0.659 and 0.704 for predicting overall survival, 0.661 and 0.711 for predicting distant metastasis-free survival, and 0.636 and 0.663 for predicting disease-free survival.Conclusions: PET/CT was more effective than MRI in identifying metastatic SCLNs, and the radiologic diagnostic criteria for metastatic lymph nodes in level IV/Vb should be re-defined. | Hao Peng Lei Chen Ling-Long Tang Wen-Fei Li Yan-Ping Mao Rui Guo Yuan Zhang Li-Zhi Liu Li Tian Xu Zhang Xiao-Ping Lin Ying Guo Ying Sun Jun Ma | 2017 | Chinese Journal of Cancer2017，36，12： | 14 |
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