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| 1 | Cloning and characterization of squalene synthase and cycloartenol synthase from Siraitia grosvenorii显示文摘Mogrosides and steroid saponins are tetracyclic triterpenoids found in Siraitia grosvenorii.Squalene synthase(SQS) and cycloartenol synthase(CAS) are key enzymes in triterpenoid and steroid biosynthesis.In this study,full-length cDNAs of SgSQS and SgCAS were cloned by a rapid amplification of cDNA-ends with polymerase chain reaction(RACE-PCR) approach.The SgSQS cDNA has a 1254 bp open reading frame(ORF) encoding 417 amino acids,and the SgCAS cDNA contains a 2298 bp ORF encoding 765 amino acids.Bioinformatic analysis showed that the deduced SgSQS protein has two transmembrane regions in the C-terminal.Both SgSQS and SgCAS have significantly higher levels in fruits than in other tissues,suggesting that steroids and mogrosides are competitors for the same precursors in fruits.Combined in silico prediction and subcellular localization,experiments in tobacco indicated that SgSQS was probably in the cytoplasm or on the cytoskeleton,and SgCAS was likely located in the nucleus or cytosol.These results will provide a foundation for further study of SgSQS and SgCAS gene functions in S.grosvenorii,and may facilitate improvements in mogroside content in fruit by regulating gene expression. | Huan Zhao Qi Tang Changming Mo Longhua Bai Dongping Tu Xiaojun Ma | 2017 | Acta Pharmaceutica Sinica B2017，7，2： | 10 |
| 2 | Cloning and expression of SgCYP450-4from Siraitia grosvenorii显示文摘CYP450 plays an essential role in the development and growth of the fruits of Siraitia grosvenorii. However, little is known about the SgCYP450-4 gene in S. grosvenorii. Here, based on transcriptome data, a full-length c DNA sequence of SgCYP450-4 was cloned by reverse transcriptasepolymerase chain reaction(RT-PCR) and rapid-amplification of c DNA ends(RACE) strategies. SgCYP450-4 is 1677 bp in length(Gen Bank accession No. AEM42985.1) and contains a complete open reading frame(ORF) of 1422 bp. The deduced protein was composed of 473 amino acids, the molecular weight is 54.01 kDa, the theoretical isoelectric point(PI) is 8.8, and the protein was predicted to possess cytochrome P450 domains. SgCYP450-4 gene was highly expressed in root, diploid fruit and fruit treated with hormone and pollination. At 10 days after treatment with pollination and hormones, the expression of SgCYP450-4had the highest level and then decreased over time, which was consistent with the development of fruits of S.Grosvenorii. Hormonal treatment could significantly induce the expression of SgCYP450-4. These results provide a reference for regulation of fruit development and the use of parthenocarpy to generate seedless fruit, and provide a scientific basis for the production of growth regulator application agents. | Dongping Tu Xiaojun Ma Huan Zhao Changming Mo Qi Tang Liuping Wang Jie Huang Limei Pan | 2016 | Acta Pharmaceutica Sinica B2016，6，6： | 3 |
| 3 | Estimating the minimum in-stream flow requirements via wetted perimeter method based on curvature and slope techniques显示文摘Under the assumptions of triangular cross section channel and uniform stable flow, an analytical solution of the minimum ecological in-stream flow requirement (MEIFR) is deduced. Based on the analytical solution, the uncertainty of the wetted perimeter method is analyzed by comparing the two techniques for the determination of the critical point on the relationship curve between wetted perimeter, P and discharge, Q. It is clearly shown that the results of MEIFR based on curvature technique (corresponding to the maximum curvature) and slope technique (slope being 1) are significantly different. On the P-Q curve, the slope of the critical point with the maximum curvature is 0.39 and the MEIFR varied prominently with the change of the slope threshold. This indicates that if a certain value of the slope threshold is not available for slope technique, curvature technique may be a better choice. By applying the analytical solution of MEIFR in the losing rivers of the Western Route South-to-North Water Transfer Project in China, the MEIFR value via curvature technique is 2.5%-23.7% of the multi-year average annual discharge, while that for slope technique is 11%-105.7%. General conclusions would rely on the more detailed research for all kinds of cross-sections. | LIU Suxia' MO Xingguo XIA Jun LIU Changming LINZhonghui MEN Baohui JI Lina | 2006 | Journal of Geographical Sciences2006，16，2： | 2 |
| 4 | An effi-cient approach to finding Siraitia grosvenorii triterpene bio-synthetic genes by RNA-seq and digital gene expression a-nalysis显示文摘 | TANG Qi MA Xiaojun MO Changming | 2011 | BMC Genomics2011，12，： | 1 |
| 5 | An efficient approach to finding Siraitia grosvenorii triterpene biosynthetic genes by RNA-seq and digital gene expression analysis显示文摘 | Qi Tang Xiaojun Ma Changming Mo | 2011 | Bmc Genomics2011，12，： | 1 |
| 6 | Green hydrogen from bio-ethanol reforming using micro plasma显示文摘Hydrogen is a kind of energy source with a characteristic of high calorific value and plasma is regarded as a good method of hydrogen production.Hydrogen produced from ethanol reforming using micro plasma can be utilized for portable applications.The study introduces water/carbon ratio,residence time,and ethanol mass flow rate as the parameters of experimental conditions,and makes ethanol conversion,gas production rate,gas product selectivity,energy consumption,and efficiency of hydrogen production per unit as the evaluation parameters of ethanol reforming reaction.The experimental results showed that(1)in the argon micro plasma:the maximum ethanol conversion of 25.3%was obtained at residence time=2.1 ms,ethanol mass flow rate=0.01 g/s and S/C=1.0,and the peak value of hydrogen production of 18.7μmol/s was achieved at residence time=2.1 ms,ethanol mass flow rate=0.05 g/s and S/C=1.0;(2)in the air micro plasma:the maximum ethanol conversion of 37.4%was obtained at residence time=2.1 ms,ethanol mass flow rate=0.01 g/s and S/C=1.0,and the peak value of hydrogen production of 36.8μmol/s was achieved at residence time=2.1 ms,ethanol mass flow rate=0.03 g/s and S/C=3.0.These experimental results showed that the microreactor can substantially reduce the volume of the device while producing a large amount of output. | ZiKe Qiu XianGe Wei JianMin Mo JiaMin Ding ChangMing Du | 2020 | Waste Disposal and Sustainable Energy2020，2，4： | 1 |
| 7 | Plasma remediation for petroleum pollution of the sea显示文摘This paper is concerned about the application of glow discharge plasma for the remediation of petroleum pollution of the sea.We used diesel fuel as the processing target and conducted a series of lab-scale studies.Several factors including the discharge voltage,gas flow rate,the concentration of NaCl,initial temperature,and initial concentration of diesel fuel can influence the performance of glow discharge plasma systems on removal efficiency of diesel fuel.To determine the optimal reaction conditions,the diesel degradation rate and energy efficiency were calculated.The experiment result for 2178 mg/L diesel fuel solution treatment was that removal efficiency of 93.3%was achieved after 12 min treatment with the energy yield of 17 g(kWh)^(−1).Higher voltage leads to larger removal efficiency,while the energy yield may be reduced.The removal efficiency of diesel fuel reached the maximum at the gas flow rate factor 700 mL/min,decreased with an increase in gas flow rate,and became a minimum value when the gas flow rate is 1000 mL/min as a result of the unstable discharge.Decreasing the concentration of sodium chloride can cause an increase in the removal efficiency of diesel fuel,because the conductivity tended to significantly increase the degradation efficiency.Moreover,temperature and initial concentration are demonstrated to have little influence on the rate constant,but higher initial concentration favors higher energy efficiency.During the remediation of diesel fuel,pH decreases to 2.76,while the conductivity of the solution and temperature are increased,especially for the temperature,which has an increase from 25℃ to 63℃. | ZiKe Qiu ZhanYi Zhang ZhiXing Mo Meng Li ChangMing Du | 2021 | Waste Disposal and Sustainable Energy2021，3，1： | 0 |
| 8 | The effect of 8-OH-DPAT and dapoxetine on gene expression in the brain of male rats during ejaculation显示文摘The 5-HT_(1A) receptor agonist 8-hydroxy-2-[di-n-propylamino] tetralin(8-OH-DPAT) promotes ejaculation of male rats, whereas dapoxetine delays this process. However, the gene expression profile of the brain at ejaculation following administrationof these two compounds has not been fully elucidated. In the present study, a transcriptomic Body Map was generated by conducting mRNA-Seq on brain samples of male Sprague–Dawley rats. The study included four groups: pre-copulatory control(CK) group,ejaculation(EJ) group, 0.5 mg/kg 8-OH-DPAT-ejaculation group(DPAT), and 60 mg/kg dapoxetineejaculation(DAP) group. The resulting analysis generated an average of approximately 47 million sequence reads. Significant differences in the gene expression profiles of the aforementioned groups were observed in the EJ(257 genes), DPAT(349 genes) and the DAP(207 genes) compared with the control rats. The results indicate that the expression of Drd1 and Slc6a3 was significantly different after treatment with 8-OH-DPAT, whereas the expression of Drd4 was significantly different after treatment with dapoxetine. Other genes, such as Wnt9b, Cdkn1 a and Fosb, exhibited significant differences in expression after the two treatments and are related to bladder cancer, renal cell carcinoma and sexual addiction. The present study reveals the basic pattern of gene expression that was activated at ejaculation in the presence of 8-OH-DPAT or dapoxetine, providing preliminary gene expression information during rat ejaculation. | Xijun Qin Xiaojun Ma Dongping Tu Zuliang Luo Jie Huang Changming Mo | 2017 | Acta Pharmaceutica Sinica B2017，7，3： | 0 |
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