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| 1 | Transactivation of the TIEG1 confers growth inhibition of transforming growth factor-β-susceptible hepatocellular carcinoma cells显示文摘AIM:To investigate the role of transforming growth factor(TGF)-β-inducible early gene 1(TIEG1) in TGF-β-induced growth inhibition in hepatocellular carcinoma(HCC) cells.METHODS:Human hepatocyte and HCC cell lines with varied susceptibilities to TGF-β1 were tested by methylthiazoletetrazolium(MTT) assay.The expression changes of Smad2,Smad3,Smad4,Smad7,TIEG1 and TIEG2 gene following treatment with TGF-β1 in a TGF-β-sensitive hepatocyte cell line(MIHA),a TGF-β-sensitive hepatoma cell line(Hep3B) and two TGF-β-insensitive hepatoma cell lines(HepG2 and Bel7404) were examined.SiRNA targeting TIEG1 was transfected into Hep3B cells and the sensitivity of cells to TGF-β1 was examined.Overexpression of TIEG1 was induced by lentiviral-mediated transduction in TGF-β1-resistant hepatoma cell lines(Bel7404 and HepG2).MTT assay and 4',6-Diamidino-2-phenylindole staining were used to identify cell viability and apoptosis,respectively.The expression level of stathmin was measured by reverse transcriptase polymerase chain reaction and Western-blotting analysis,and stathmin promoter activity by TIEG1 was monitored by a luciferase reporter gene system.RESULTS:TIEG1 was significantly upregulated by TGF-β1 in the TGF-β1-sensitive HCC cell line,Hep3B,but not in the resistant cell lines.The suppression of TIEG1 by siRNAs decreased the sensitivity of Hep3B cells to TGF-β1,whereas the overexpression of TIEG1 mediated growth inhibition and apoptosis in TGF-β1-resistant HCC cell lines,which resembled those of TGF-β1-sensitive HCC cells treated with TGF-β1.Our data further suggested that stathmin was a direct target of TIEG1,as stathmin was signif icantly downregulated by TIEG1 overexpression,and stathmin promoter activity was inhibited by TIEG1 in a dose-dependent manner.CONCLUSION:Our data suggest that transactivation of TIEG1 conferred growth inhibition of TGF-β-susceptible human HCC cells. | Lei Jiang Yiu-Kay Lai Jin-Fang Zhang Chu-Yan Chan Gang Lu Marie CM Lin Ming-Liang He Ji-Cheng Li Hsiang-Fu Kung | 2012 | World Journal of Gastroenterology2012，18，17： | 13 |
| 2 | Risk factors and natural history of breast cancer in younger Chinese women显示文摘AIM: To investigate the age differences in the risk factors, clinicopathological characteristics and patterns of treatment of female breast cancer patients. METHODS: Seven thousand one hundred and fiftytwo women with primary breast cancer from the Hong Kong Breast Cancer Registry were recruited after receiving patients' consent, they were asked to complete standardized questionnaires which captured their sociodemographic characteristics and risk factors associated with breast cancer development. Among them, clinicopathological data and patterns of treatment were further collected from medical records of 5523 patients with invasive breast cancers. Patients were divided into two groups according to the age at diagnosis: younger(< 40 years old) vs older patients(≥ 40 years old) for subsequent analyses.RESULTS: Analysis on the sociodemographic characteristics and exposure to risk factors were performed on 7152 women with primary breast cancer and the results revealed that younger patients were more likely to have unhealthy lifestyles; these include a lack of exercise(85.4% vs 73.2%, P < 0.001), having high stress in life(46.1% vs 35.5%, P < 0.001), having dairy/meat-rich diets(20.2% vs 12.9%, P < 0.001),having alcohol drinking habit(7.7% vs 5.2%, P = 0.002). Younger patients were also more likely to have hormone-related risk factors including nulliparity(43.3% vs 17.8%, P < 0.001) and an early age at menarche(20.7% vs 13.2%, P < 0.001). Analyses on clinicopathological characteristics and patterns of treatment were performed on 5523 women diagnosed with invasive breast cancer. The invasive tumours in younger patients showed more aggressive pathological features such as having a higher percentage of grade 3 histology(45.7% vs 36.5%, P < 0.001), having a higher proportion of tumours with lymphovascular invasion(39.6% vs 33.2%, P = 0.003), and having multifocal disease(15.7% vs 10.3%, P < 0.001); they received different patterns of treatment than their older counterparts.CONCLUSION: Younger patients in Hong Kong are more likely to encounter risk factors associated with breast cancer development and have more aggressive tumours than their older counterparts. | Winnie Yeo Hang-Mei Lee Amy Chan Emily YY Chan Miranda CM Chan Keeng-Wai Chan Sharon WW Chan Foon-Yiu Cheung Polly SY Cheung Peter HK Choi Josette SY Chor William WL Foo Wing-Hong Kwan Stephen CK Law Lawrence PK Li Janice WH Tsang Yuk Tung Lorna LS Wong Ting-Ting Wong Chun-Chung Yau Tsz-Kok Yau Benny CY Zee | 2014 | World Journal of Clinical Oncology2014，5，5： | 4 |
| 3 | 2013年美国心脏病学会/美国心脏协会胆固醇管理指南在美国心脏病学实践中的应用显示文摘2013年美国心脏病学会/美国心脏协会(American College of Cardiology/American Heart Association,ACC/AHA)胆固醇管理指南建议在符合治疗标准的患者中采用中等强度至高强度他汀类药物治疗。该研究的目的在于审查2013年ACC/AHA指南在美国心脏病学实践中的应用情况。 | 刘莉 叶鹏 Pokharel Y Tang F Jones PG Nambi V Bittner VA Hira RS Nasir K Chan PS Maddox TM Oetgen WJ Heidenreich PA Borden WB Spertus JA Petersen LA Ballantyne CM Virani SS | 2017 | 中华高血压杂志2017，25，6： | 2 |
| 4 | Minimum graft volume for successful adult-to-adult living donor liver transplantation for fulminant hepatic failure显示文摘 | Lo CM Fan ST Chan JK | 1996 | Transplantation1996，62，5： | 1 |
| 5 | Comparison of eye-tracking success in laser in situ keratomileusis after flap creation with 2 femtosecond laser models 显示文摘 | Luengo Gimeno F Chan CM Li L | 2011 | J Cataract Refract Surg2011，37，3： | 1 |
| 6 | A randomized,controlled trial of postoperative adjuvant interferon therapy after resection of hepatocellular carcinoma显示文摘 | Lo CM Liu CL Chan SC | | 0，，06： | 1 |
| 7 | Tissue Doppler echocardiographic evidence of reverse remodeling and improved synchronicity by simultaneously delaying regional contraction after biventriculur pacing therapy in heart failure显示文摘 | Yu CM Chan E Sanderson JE | 2002 | Circulation2002，105，4： | 1 |
| 8 | Purification of transferrins and lactoferrin using DEAE affi- gel blue 显示文摘 | Chung CM Chan SL Shimizu S | 1991 | Int J Biochem1991，23，： | 1 |
| 9 | Biventricular pacing in patients with bradyeardia and normal ejection fraction显示文摘 | Yu CM Chan JY Zhang Q | 2009 | N Engl J Med2009，361，22： | 1 |
| 10 | Safety of duct-to-duct biliary reconstruction in right-lobe live-donor liver transplantation without biliary drainage显示文摘 | Liu CL Lo CM Chan SC | 2004 | Transplantation2004，77，5： | 1 |
| 11 | Compliance with home non- invasive ventilation显示文摘 | Cheng SL Chan VL Chu CM | 2012 | Respirology2012，17，4： | 1 |
| 12 | Diagnostics and epidemiology in ventilator-associated pneumonia显示文摘 | Shorr AF Chan CM Zilberberg MD | 2011 | Ther Adv Respir Dis2011，5，2： | 1 |
| 13 | The validation and reproducibility of the pulmonary embolism severity index显示文摘 | Chan CM Woods C Shorr AF | 2010 | J Thromb Haemost2010，8，7： | 1 |
| 14 | Contrast echocardiography grading predicts pulmonary arteriovenous malformations on CT显示文摘 | Zukotynski K Chan RP Chow CM | 2007 | Chest2007，132，1： | 1 |
| 15 | Left ventricular systolic asynchrony after acute myocardial infarction in patients with narrow QRS complexes显示文摘 | Zhang Y Chan AK Yu CM | 2005 | Am Heart J2005，149，3： | 1 |
| 16 | Asthma,atopy and tuberculin responses in Chinese schoolchildren in Hong Kong显示文摘 | Wong GW Hui DS Tam CM Chan HH Fok TF Chan-Yeung M | 2001 | Thorax2001，56，： | 1 |
| 17 | Foreign DNA transmission by ICSI: injection of spermatozoa bound with exogenous DNA results in embryonic GPF expression and live Rhesus monkey births显示文摘 | CHAN AW LUETJENS CM DOMINKO T | 2000 | Mol Hum Reprod2000，6，1： | 1 |
| 18 | Biventrieular pacing in patients with bradycardia and normal ejection fraction显示文摘 | Yu CM Chan JY Zhang Q | 2009 | N Engl J Med2009，361，22： | 1 |
| 19 | Posterior reversible eneephalopathy syndrome with vasospasm in a postpartum woman after postdural puncture headache following spinal anesthesia 显示文摘 | Ho CM Chan KH | 2007 | Anesth Analg2007，105，3： | 1 |
| 20 | Two-port laparoscopic chole-cystectomy :initial results of a modified technique显示文摘 | Poon CM Chan KW Ko CW | 2002 | J Laparoen-dosc Adv Surg Tech A2002，12，4： | 1 |
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