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| 1 | Probiotic metabolites from Bacillus coagulans GanedenBC30^(TM) support maturation of antigen-presenting cells in vitro显示文摘AIM:To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.METHODS:Ganeden Bacillus coagulans 30(GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite(MET) fraction.A second fraction was made to generate a crude cell-wall-enriched fraction,by centrifugation and lysis,followed by washing.A preparation of MET was subjected to size exclusion centrifugation,generating three fractions:< 3 kDa,3-30 kDa,and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell(PBMC) as a source of antigen-presenting mononuclear phagocytes.The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14,CD16,CD80 and CD86 and analyzed by flow cytometry.RESULTS:Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes.The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells,and this property was associated with the high molecular weight metabolite fraction.Changes were also seen for the dendritic cell maturation markers CD80 and CD86.On CD14dim cells,an increase in both CD80 and CD86 expression was seen,in contrast to a selective increase in CD86 expression on CD14bright cells.The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation.The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.CONCLUSION:The data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells,important for immunological decision-making. | Kathleen F Benson Kimberlee A Redman Steve G Carter David Keller Sean Farmer John R Endres Gitte S Jensen | 2012 | World Journal of Gastroenterology2012，18，16： | 8 |
| 2 | Driven pile in clay the effect of installation and subsequent consolidation显示文摘 | Randolph M F Carter J P Wroth C P | 1979 | Geotechnique1979，29，4： | 2 |
| 3 | Driven Piles in clay - the effects of installation and subsequent consolidation显示文摘 | RANDOLPH M F CARTER J P WROTH C P | 1979 | Geotechnique1979，29，4： | 1 |
| 4 | Driven piles in clay-the effects of installation and sub- sequent consolidation显示文摘 | RANDOLPH M F CARTER J P & WROTH C P | 1979 | Geotechnique1979，29，4： | 1 |
| 5 | Underwriter reputation,initial returns,and the long-run performance of IPO stocks显示文摘 | Carter R Dark F Singh A | | 0，，53： | 1 |
| 6 | Progesterone-regula- ted changes in endometrial gene expression contribute to advanced conceptus development in cattle显示文摘 | Forde N Carter F Fair T | 2009 | Biol Reprod2009，81，4： | 1 |
| 7 | Epidemiology and outcome of mould infections in hematopoietic stem cell transplant recipients显示文摘 | Marr K A Carter R A Crippa F | 2002 | Clin Infect Dis2002，34，： | 1 |
| 8 | Stress and pore pressure changes in clay during and after the expansion of cylindrical cavity 显示文摘 | CARTER J P RANDOLPH M F WROTH C P | 1979 | Int J for Num and Analy Meth in Geomech1979，3，2： | 1 |
| 9 | Ethanol impaired neuronal migration is associated with reduced aspartyl-asparaginylbeta-hydroxylase expression显示文摘 | Carter J J Tong M Silbermann E Lahousse S A Ding F F Longato L Roper N Wands J R | | 0，，03： | 1 |
| 10 | Geographic Information Systems for Geoscientists Modelling with GIS, Pergamon 显示文摘 | Bonham - Carter G F | 1994 | Ontario1994，，： | 1 |
| 11 | Driven Piles in Clay: The Effects of Installation and Subsequent Consolidation 显示文摘 | Randolph M F Carter J P Wroth C P | 1979 | Geotechnique1979，29，4： | 1 |
| 12 | Testing reported associa-tions of genetic risk factors for oral clefts in a large Irish study popula-tion显示文摘 | Carter TC Molloy AM Pangilinan F | 2010 | Birth Defects Res A Clin Mol Teratol2010，88，2： | 1 |
| 13 | Iterative methods for solving the dual formulation arising from image restoration 显示文摘 | Chart T F Chen K Carter J L | 2007 | Electronic Transactions on Numerical Analysis2007，26，2： | 1 |
| 14 | Epidemiology and out come of mould infections in hematopoietic stem cell trans plant recipients显示文摘 | Marr KA Carter RA Crippa F | 2002 | Clin Infect Dis2002，34，7： | 1 |
| 15 | The effects of irrigation on out- comes in cases of perforated appendicitis in children显示文摘 | Hartwich J E Carter R F Wolfe L | 2013 | J Surg Res2013，180，2： | 1 |
| 16 | The Utilization of E - Government Sen, ices Citizen Trust, Innovation and Acceptance Factors 显示文摘 | Carter L Belange F | 2005 | Information System Journal2005，15，1： | 1 |
| 17 | On the action of a locomotive driving wheel 显示文摘 | Carter F W | 1926 | Proceedings of the Royal Society of London1926，112，： | 1 |
| 18 | Hole doping of the CuO2 chains(La,Sr,Ca)14Cu24 O41显示文摘 | CARTER S A BATLOGG B CAVA R J KRAJEWSKI J J PECK JR W F RICE T M | 1996 | Phys Rev Lett1996，77，7： | 1 |
| 19 | Driven Piles in Clay--The Effects of Installation and Subsequent Consolidation显示文摘 | RANDOLPH M F CARTER J P WROTH C P | 1979 | Geotechnique1979，29，4： | 1 |
| 20 | The utilization of e-government serv- ices: citizen trust, innovation and acceptance factors 显示文摘 | Carter L Belange F | 2005 | In- formation Systems Journal2005，15，1： | 1 |
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