|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 2 | Antigenic drift of the pandemic 2009 A (H1N1) influenza virus in a ferret model显示文摘 | Guarnaccia T Carolan L A Maurer-Stroh S | 2013 | PLoS Path2013，9，10： | 1 |
| 3 | Role of epidermal growth factor in bovine oocyte maturation and preimplantation embryo development in vitro 显示文摘 | Lonergan P Carolan C Langendonckt A V et at | 1996 | Biol Reprod1996，54，： | 1 |
| 4 | A place to be: the role of clubhouses in facilitating social support 显示文摘 | CAROLAN M ONAGA E PERNICE-DUCA F | 2011 | Psychiatr MCKAY programs: Psychiatr Rehabil J2011，35，2： | 1 |
| 5 | Whole brain radiotherapy withhippocampal avoidance and simultaneous integrated boost for 1-3brain metastases: a feasibility study using volumetric modulated arctherapy显示文摘 | Hsu F Carolan H Nichol A | 2010 | Int J Radiat Oncol Biol Phys2010，76，5： | 1 |
| 6 | Making Patents and Intellectual Property Work: The Asymmetrical “ Harmonization” of TRIPS 显示文摘 | CAROLAN M S | 2008 | Organization & Environment2008，21，3： | 1 |
| 7 | Costs and benefits of more learner freedom recta-analyses of exploratory and learner control training methods 显示文摘 | CAROLAN T F HUTCHINS S D WICKENS C D | 2014 | Human Factors= The Journal of the Human Factors and Ergonomics Society2014，，00： | 1 |
| 8 | Cryptic bumblebee species:consequences for conservation and the trade in greenhouse pollinators显示文摘 | Williams PH An J Brown MJF Carolan JC Goulson D Huang J Ito M | | 0，，03： | 1 |
| 9 | Planar versus SPET imaging in the assessment of condylar growth显示文摘 | Chan WL Carolan MG Fernandes VB | | 0，，03： | 1 |
| 10 | Clinical phenotypes of chronic obstruc- tive pulmonary disease and asthma: recent advances 显示文摘 | Carolan B J Sutherland ER | 2013 | J Allergy Clin Immunol2013，131，3： | 1 |
| 11 | Proteomic evaluation of citrate-coated silver nanoparticles toxicity in Daphnia magna显示文摘 | Rainville LC Carolan D Varela AC | 2014 | Analyst2014，139，7： | 1 |
| 12 | Diabetes nurse educators, experiences of pro-viding care for women, with gestational diabetes melli-tus?from disadvantaged backgrounds 显示文摘 | Carolan M | 2013 | J Clin Nurs2013，23，910： | 1 |
| 13 | Maternal age,ethnicity and gestational diabetes mellitus显示文摘 | Carolan M Davey MA Biro MA | 2012 | Midwifery2012，28，6： | 1 |
| 14 | Disparate oxidant gene expression of airway epithelium compared to alveolar macro- phages in smokers 显示文摘 | Carolan BJ Harvey BG Hackett NR | 2009 | Respir Res2009，10，： | 1 |
| 15 | Advanced mater- nal age and obstetric morbidity for women giving birth in Victoria;A population-based study显示文摘 | Biro MA Davey MA Carolan M | 2012 | Aust NZJ Obstet Gynaecol2012，52，3： | 1 |
| 16 | Impaction grafting for bone defects in revision total knee arthroplasty 显示文摘 | Lotke PA Carolan GF Puri N | 2006 | Clin Orthop Relat Res2006，446，6： | 1 |
| 17 | Up-regulation of expression of the ubiquitin carboxyl-terminal hydrolase L1 gene in human airway epithelium of cigarette smokers显示文摘 | Carolan B J Heguy A Harvey B G | 2006 | Cancer Res2006，66，10： | 1 |
| 18 | Older maternal age and intervention in labor: a population-based study comparing old- er and younger first- time mothers in Victoria 显示文摘 | Carolan M Davey MA Biro MA | 2011 | Australia Birth2011，38，1： | 1 |
| 19 | Up-regulation of ex- pression of the ubiquitin carboxyl-terminal hydrolase L1 gene in human airway epithelium of cigarette smokers 显示文摘 | Carolan B J Heguy A Harvey BG | 2006 | Cancer Res2006，66，10： | 1 |
| 20 | Muhiple infections with seasonal influenza A virus induce cross-protective immunity against A ( H1N1 ) pandemic influenza virus in a ferret model 显示文摘 | Laurie KL Carolan LA Middleton D | 2010 | J Infect Dis2010，202，7： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员