| 2 | Antibody upstream sequence diversity and its biological implications revealed by repertoire sequencing显示文摘The sequence upstream of the antibody variable region(antibody upstream sequence[AUS])consists of a 5′untranslated region(5′UTR)and a preceding leader region.The sequence variations in AUS affect antibody engineering and PCR based antibody quantification and may also be implicated in mRNA transcription and translation.However,the diversity of AUSs remains elusive.Using 5′rapid amplification of cDNA ends and high-throughput antibody repertoire sequencing technique,we acquired full-length AUSs for human,rhesus macaque,cynomolgus macaque,mouse,and rat.We designed a bioinformatics pipeline and identified 3307 unique AUSs,corresponding to 3026 and 1457 unique sequences for 5′UTR and leader region,respectively.Comparative analysis indicated that 928(63.69%)leader sequences are novel relative to those recorded in the international ImMunoGeneTics information system.Evolutionarily,leader sequences are more conserved than 5′UTR and seem to coevolve with their downstream V genes.Besides,single-nucleotide polymorphisms are position dependent for leader regions and may contribute to the functional reversal of the downstream V genes.Finally,the AUGs in AUSs were found to have little impact on gene expression.Taken together,our findings can facilitate primer design for capturing antibodies efficiently and provide a valuable resource for antibody engineering and molecule-level antibody studies. | Yan Zhu Xiujia Yang Cuiyu Ma Haipei Tang Qilong Wang Junjie Guan Wenxi Xie Sen Chen Yuan Chen Minhui Wang Chunhong Lan Deqiang Sun Lai Wei Caijun Sun Xueqing Yu Zhenhai Zhang | 2021 | Journal of Genetics and Genomics2021，48，10： | 0 |
| 3 | Study on Element Contents in Tobacco Leaves from Different Positions of Tobacco CB-1显示文摘[Objectives]This study was conducted to clarify the differences and abundance or deficiency of various elements in tobacco leaves from different positions of CB-1 in Zhangping City,so as to guide the production of precision fertilization.[Methods]The element contents of CB-1 were determined by an X-ray fluorescence spectrometer.[Results]In terms of the contents of carbon,the upper leaves(45.73%)were significantly higher than the cutters(41.53%),and the lower leaves(43.15%)had no significant differences from both of them.For the content of oxygen element,there was no significant difference between the cutters(44.77%)and the lower leaves(43.40%),both of which were significantly higher than the upper leaves(40.75%).The contents of nitrogen,phosphorus,sulfur,boron and nickel all increased with the increase of the position,and they were significantly higher in the upper leaves than in the cutters and lower leaves.The contents of potassium,calcium,magnesium,chlorine and silicon all decreased with the increase of the position,and the lower leaves were significantly higher than the cutters and upper leaves.The contents of aluminum,titanium and chromium increased significantly with the increase of the position.The contents of strontium,zirconium and arsenic decreased significantly with the increase of the position.The element contents of tobacco leaves had significant or extremely significant differences between different parts of CB-1 in Zhangping City mostly.The contents of nitrogen,potassium,calcium,sulfur,chlorine,molybdenum and nickel in tobacco leaves were appropriate,and the contents of manganese and iron were too high,while the contents of phosphorus and copper were slightly low.The contents of magnesium,silicon,boron and sodium were too low.[Conclusions]This study can guide targeted fertilization and regulation of element contents in the tobacco leaves from different parts in production,and is of great significance to improving the production,quality and industrial availability of CB-1. | Caijun XIE Shilei ZHANG Deqing CHEN Penghui LYU Wensheng LIN Jianrong ZHENG Jinchai YU Qiuying CAO Siyi CHENG Xueqin ZHANG Yongxin FENG Zenghan XU | 2022 | Agricultural Biotechnology2022，11，6： | 0 |
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