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2篇 您的检索式:作者名="CUI Yebing"
    题名 作者 年代 出处 被引量
1Enrichment,expansion,and identification of stem-like cells from hepatic carcinoma HepG2 cells显示文摘Objective:To establish a method for enriching,culturing and identifying stem-like subpopulation from human hepatic carcinoma cells,and to explore its biological properties.Methods:HepG2cells were cultured in cancer stem cell(CSC)medium to form spheroids.The stem-like HepG2cells obtained from tumor spheroids were then expanded.Flow cytometry was used to detect the expression of CD90 and CD133on the surface of stem-like HepG2 cells.The in vitro colony forming ability and in vivo tumorigenicity were detected by clone formation assay and tumorigenesis assay.Results:HepG2cells could grow in suspension and form spheroids in CSC medium.The stem-like cells had the ability of self-renewal and proliferation.The expression of CD90and CD133on the surface of these stem-like cells were higher than those of parental HepG2cells(P<0.01).The colony formation ability of stem-like cells was higher than that of parental HepG2cells.When injected with 1×106 cells,stemlike cells could form tumors earlier than parental cells in nude mice.The stem-like cells’tumorigenesis rate was higher and the tumor size was larger than those of parental HepG2cells(P<0.01).Conclusion:The suspension sphere culture method could enrich stem-like cells from HepG2cells.The obtained stem-like cells possessed the properties of self-renewal in vitro and tumorigenicity in vivo.Biyu Cui Yebing Pang Xuehui Zhang Jing Lan Xilei Li Manya Wu Xing Guo Rong Liang Yan Feng Xiaoling Luo 2017广西医科大学学报2017,34,1:0
2大功率高电压电动伺服系统在运载火箭上的应用研究显示文摘The 30 kW high-power electric servo system used in the solid booster of the Long March 6 A(LM-6 A)launch vehicle is introduced,and the function,composition of the system as well as its constituent equipments are detailed.To solve the problem of out-of-tolerance in the system dynamic characteristics,an advanced correction network algorithm architecture and double notch filter were designed.Experimental verification was conducted to prove that the dynamic characteristics requirement under multiple operating conditions could be met.FENG Wei CUI Yebing WANG Yaoyao YU Ge SONG Shuwei CHEN Shuheng GU Dawei 2022Aerospace China2022,23,2:0
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