|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A fast and effective method to perform paternity testing for Wolong giant pandas显示文摘Captive populations provide a precious genetic resource for endangered animals and a source of individuals for reintroduction to depleted habitats. Therefore, accuracy in determining paternity is of vital importance for managing captive populations and in selecting representative individuals of known genetic characteristics for release. In this study, we established a fast and effective method to conduct paternity testing for captive giant pandas in the Wolong population. This technique uses two highly polymor phic microsatellites initially, subsequent use of five less polymorphic markers and then paternity exclusion testing carried out using the giant panda paternity exclusion program we have developed. Our results revealed that (1) both sets of markers success- fully identified the real fathers in 25 cases of paternity testing and (2) the success rate of paternity exclusion varied with the degree of polymorphism of the markers used. Subsequently, we conducted correlation analysis between the success rates of paterni- ty identification with these markers, parameters of genetic diversity and tests of neutrality. We found that the paternity exclusion power of microsatellites was significantly correlated with the number of alleles (Na), expected heterozygosity (HE) and observed homozygosity statistic (FO) (all P < 0.05). From this, we developed a new variable, Na?HE/FO, showing a highly significant positive correlation with the resolution power of microsatellites (P = 0.001). Moreover, the first two highly polymorphic loci gave a 100% success rate of excluding non-paternal males because they yielded higher values of Na?HE/FO than the other five less pol- ymorphic markers. Thus, the Na?HE/FO parameter appears suitable to serve as a criterion for selecting microsatellite markers, which could be used for high-resolution molecular techniques of paternity determination among a range of captive animals besides giant pandas. | LI DeSheng CUI HengMin WANG ChengDong LING ShanShan HUANG Zhi ZHANG HeMin | 2011 | Chinese Science Bulletin2011，56，24： | 4 |
| 2 | Pathology of thymus, spleen and bursa in zinc deficient ducklings 显示文摘 | Cui Hengmin Fang Jing Peng Xi | 2003 | Avian Pathology2003，32，3： | 1 |
| 3 | Increased apoptotic lymphocyte population in the spleen of young chickens fed on diets high in molybdnum显示文摘 | Yang Fan Cui Hengmin Xiao Jie | 2011 | Biol Trace Element Res2011，140，3： | 1 |
| 4 | Effects of Aflatoxin B 1 Exposure and Sodium Selenite Supplementation on the Histology, Cell Proliferation, and Cell Cycle of Jejunum in Broilers显示文摘 | Shengqiang Zhang Xi Peng Jing Fang Hengmin Cui Zhicai Zuo Zhengli Chen | 2014 | Biological Trace Element Research2014，，1： | 1 |
| 5 | Pathology of thymus,spleen and bursa in zinc deficient ducklings显示文摘 | Cui Hengmin Fang Jing Peng Xi | 2003 | Avian Pathology2003，32，3： | 1 |
| 6 | Effects of Chinese Herbal Compound on the Blood Physiological Indices and Cytokines of Myelosuppressive Mice显示文摘[Objective] The research aimed to study the effects of Chinese herbal compound on the blood physiological indices and cytokines of myelosuppressive mice. [Method] Myelosuppressive mice model was established by intraperitoneal injection of 80 mg / kg cyclophosphamide. Chinese herbal compound was composed of Houttuynia cordata,Taraxacum mongolicum,Citrus reticulata peel,Atractylodes chinensis,Paeonia sterniana,Atractylodes macrocephala and Angelica sinensis. The effects of Chinese herbal compound and Astragalus polysaccharide at different doses on the blood physiological indices and hematopoietic growth factors of myelosuppressive mice were discussed. [Result] Myelosuppressive mice model was successfully established. The total white blood cell count,total neutrophile granulocyte count,total lymphocyte count,total platelet count and the contents of serum interleukin-6 and erythropoietin in myelosuppressive mice were significantly decreased. The total erythrocyte count,the contents of hemoglobin and interleukin-3 were decreased,without significant difference. The blood physiological indices and the contents of interleukin-3 and erythropoietin in myelosuppressive mice could be improved by intragastric administration of Astragalus polysaccharide and Chinese herbal compound at different doses for 3 days or 7 days. The effect of 20 g / kg Chinese herbal compound was the best after administration for 3 days,and the effect of 10 g / kg Chinese herbal compound was the best after administration for 7 days. The total white blood cell count,total neutrophile granulocyte count,percentage of neutrophile granulocyte,total lymphocyte count,total count of middle cells,percentage of middle cells,total platelet count,contents of interleukin-3 and erythropoietin in myelosuppressive mice could be extremely significantly improved by intragastric administration of 10 g / kg Chinese herbal compound for 7 days( P <0. 01),and the reduction of red blood cell count,hematocrit,the contents of hemoglobin and interleukin-6 induced by cyclophosphamide could be inhibited( P <0. 01). [Conclusion] Chinese herbal compound could improve the hematopoietic function of myelosuppressive mice induced by cyclophosphamide and its effect was better than Astragalus polysaccharide. 20 g / kg Chinese herbal compound reacted fast and the reaction of 10 g / kg Chinese herbal compound was slow,but its efficacy was lasting. | Mu Xiaodong Zuo Zhicai Gan Meng Cui Hengmin Peng Xi Wang Ya Deng Junliang Xu Zhiwen | 2014 | Animal Husbandry and Feed Science2014，6，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员