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| 1 | Stable chloroplast transformation of immature scutella and inflorescences in wheat (Triticum aestivum L.)显示文摘在小麦的叶绿体转变被 scutella 的轰炸分别地从不成熟的胚胎和不成熟的开花期完成。小麦叶绿体地点特定的表示向量, pBAGNRK,被放包含新霉素 phosphotransferase II (nptII ) 和绿色的一个表示盒子构造荧光灯蛋白质(gfp ) 作为选择和记者基因,分别地在在小麦的 atpB 和 rbcL 之间的 intergenic 分隔符叶绿体染色体。在 plastome 的 gfp 基因的集成被聚合酶链反应(PCR ) 识别分析并且作为一根探针的用 gfp 基因的南部的弄污。GFP 蛋白质的表示被西方的污点检验。三积极 transformants 被获得, atpB 和 rbcL (指向的地点) 的部分碎片的南部的污点探查证实他们之一是 homoplasmic。GFP 荧光的稳定的表示被共焦的显微镜学从 T1 子孙幼苗在叶纸巾证实。gfp 基因的 PCR 分析也在 T1 子孙证实了 transgene 的继承。这些结果加强小麦叶绿体转变的可行性并且也通过叶绿体转变在小麦为重要农学的特点的介绍给一个新奇方法。 | Cuiju Cui FeiSong Yi Tan Xuan Zhou Wen Zhao Fengyun Ma Yunyi Liu Javeed Hussain Yuesheng Wang Guangxiao Yang Guangyuan He | 2011 | Acta Biochimica et Biophysica Sinica2011，43，4： | 10 |
| 2 | Characterization of Genome-Wide Microsatellites of Saccharina japonica Based on a Preliminary Assembly of Illumina Sequencing Reads显示文摘Microsatellites or simple sequence repeats(SSR) function widely and locate dependently in genome. However, their characteristics are often ignored due to the lack of genomic sequences of most species. Kelp(Saccharina japonica), a brown macroalga, is extensively cultured in China. In this study, the genome of S. japonica was surveyed using an Illumina sequencing platform, and its microsatellites were characterized. The preliminarily assembled genome was 469.4 Mb in size, with a scaffold N_(50) of 20529 bp. Among the 128370 identified microsatellites, 90671, 25726 and 11973 were found in intergenic regions, introns and exons, averaging 339.3, 178.8 and 205.4 microsatellites per Mb, respectively. These microsatellites distributed unevenly in S. japonica genome. Mononucleotide motifs were the most abundant in the genome, while trinucleotide ones were the most prevalent in exons. The microsatellite abundance decreased significantly with the increase of motif repeat numbers, and the microsatellites with a small number of repeats accounted for a higher proportion of the exons than those of the intergenic regions and introns. C/G-rich motifs were more common in exons than in intergenic regions and introns. These characteristics of microsatellites in S. japonica genome may associate with their functions, and ultimately their adaptation and evolution. Among the 120140 pairs of designed microsatellite primers, approximately 75% were predicted to be able to amplify S. japonica DNA. These microsatellite markers will be extremely useful for the genetic breeding and population evolution studies of kelp. | ZHANG Linan PENG Jie LI Xiaojie CUI Cuiju SUN Juan YANG Guanpin | 2016 | Journal of Ocean University of China2016，15，3： | 2 |
| 3 | Copper-catalyzed asymmetric propargylic substitution of anthrones and propargylic esters显示文摘Anthrones are key structural motifs in many natural products, bioactive compounds and pharmaceutical chemicals. Earth-abundant-metal-catalyzed asymmetric functionalization of anthrones has not proved to be viable. Herein, we disclosed a highly enantioselective propargylic substitution of anthrones with propargylic esters using copper salts with chiral N, N, P-ligand. This strategy is amenable to a broad range of substrates, uses readily available starting materials, provides excellent yields with remarkable enantioselectivity under mild conditions, and enables attractive products diversification routes. | Zhiheng Li Danran Li Huaming Xiang Jian Huang Yinuo Zheng Cuiju Zhu Xiuling Cui Chao Pi Hao Xu | 2022 | Chinese Chemical Letters2022，33，2： | 0 |
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