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| 1 | ISCHEMIA AND REPERFUSION REDUCE THE ENDOGENOUS BASIC FIBROBLAST GROWTH FACTOR (bF GF) IN RATSKELETAL MUSCLES显示文摘Polyclonal antibodies directed against human recombinant basic fibroblast growth factor (bFGF) were used in immunohistochemical studies to localize this growth factor in normal and wounded rat skeletal muscles. bFGF immunoreactivity was found mainly in the extracellular matrix, primarily in the endomysium, including the heparin-containing basal lamina and also in the capillary basal membrane of both normal and wounded muscles, however the signal intensity was much stronger in normal muscles. After 4-hour ischemia, about 40% of skeletal muscle fibers lost their bFGF immunoreactivity. Muscles which experienced 4-hour ischemia and 24 reperfusion had only a weaker bFGF immunoreactivity. The pathological results supported the concept of destroyed cell connection and fiber necrosis in ischemia and reperfused muscles. The mechanisms involved in this reduced concentration of bFGF in wounded muscles included oxygen radical activation, inflammatory response and reduced secretion of endogenous bFGF. These results | 傅小兵 Cuevas P Gimenez-Gallego G 田惠民 盛志勇 | 1995 | Chinese Medical Journal1995，，9： | 3 |
| 2 | Preclinical evaluation of azathioprine plus buthionine sulfoximine in the treatment of human hepatocarcinoma and colon carcinoma显示文摘AIM: To evaluate the efficacy and the safety of azathioprine (AZA) and buthionine sulfoximine (BSO) bylocalized application into HepG2 tumor in vivo.METHODS: Different hepatoma and colon carcinoma cell lines (HepG2, HuH7, Chang liver, LoVo, RKO, SW-48, SW-480) were grown in minimal essencial medium supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained in a humidified 37 ℃ incubator with 5% CO2. These cells were pretreated with BSO for 24 h and then with AZA for different times. We examined the effects of this combination on some proteins and on cellular death. We also studied the eff icacy and the safety of AZA (6 mg/kg per day) and BSO (90 mg/kg per day) in HepG2 tumor growth in vivo using athymic mice. We measured safety by serological markers such as aminotransferases and creatine kinase.RESULTS: The in vitro studies revealed a new mechanism of action for the AZA plus BSO combination in the cancer cells compared with other thiopurines (6-mercaptopurine, 6-methylmercaptopurine, 6-thioguanine and 6-methylthioguanine) in combination with BSO. The cytotoxic effect of AZA plus BSO in HepG2 cells resulted from necroptosis induction in a mitochondrial-dependent manner. From kinetic studies we suggest that glutathione (GSH) depletion stimulates c-Jun amino-terminal kinase and Bax translocation in HepG2 cells with subsequent deregulation of mitochondria (cytochrome c release, loss of membrane potential), and proteolysis activation leading to loss of membrane integrity, release of lactate dehydrogenase and DNA degradation. Some of this biochemical and cellular changes could be reversed by N-acetylcysteine (a GSH replenisher). In vivo studies showed that HepG2 tumor growth was inhibited when AZA was combined with BSO.CONCLUSION: Our studies suggest that a combination of AZA plus BSO could be useful for localizedtreatment of hepatocellular carcinoma as in the currently used transarterial chemoembolization method. | Borja Hernández-Breijo Jorge Monserrat Sara Ramírez-Rubio Eva P Cuevas Diana Vara Inés Díaz-Laviada M Dolores Fernández-Moreno Irene D Román Javier P Gisbert Luis G Guijarro | 2011 | World Journal of Gastroenterology2011，17，34： | 2 |
| 3 | Dobesilate inhibits the activation of signal transducer and activator of transcription 3, and the expression of cyclinD1 and bcl-XL in glioma cell 显示文摘 | Cuevas P Diaz-Gonzalez D Sanchez L | 2006 | Neurol Res2006，28，2： | 1 |
| 4 | Meningioma transcript profiles reveal deregulated Notch signaling pathway 显示文摘 | Cuevas IC Slocum AL Jun P | 2005 | Cancer Res2005，65，12： | 1 |
| 5 | Block matching algorithm for motion estimation based on Artificial Bee Colony (ABC)显示文摘 | Erik Cuevas Daniel Zaldívar Marco Pérez-Cisneros Humberto Sossa Valentín Osuna | 2012 | Applied Soft Computing Journal2012，，： | 1 |
| 6 | Serum levels of basic fibroblast growth factor in acut myocardial infarction 显示文摘 | Cuevas P Barrios V Gimenez GG | 1997 | Fur J Med Res1997，2，： | 1 |
| 7 | Fibroblast growth factors and cerebral ischemia 显示文摘 | Cuevas P Carceller F Gimenez G | 1994 | Neurol Res1994，16，： | 1 |
| 8 | Luria-delbruck esti- mation of turnip mosaic virus mutation rate in vivo显示文摘 | de la Iglesia F Martinez F Hillung J Cuevas J M Gerrish P J DarOs J A Elena S F | 2012 | Virology2012，86，6： | 1 |
| 9 | Peripheral nerve regeneration by bone marrow stromal cells显示文摘 | Cuevas P Carceller F Dujovny M | 2002 | Neurol Res2002，24，7： | 1 |
| 10 | Imaging of uncommon retro peritoneal masses显示文摘 | Rajiah P Sinha R Cuevas C | 2011 | Radiographics2011，31，4： | 1 |
| 11 | TPR-S analy- sis of the catalytic behavior of Ru/AI203 catalysts in indus- trial conditions 显示文摘 | Castillo-Villal6n P Ramirez J Cuevas R | 2005 | Catal Today2005，，： | 1 |
| 12 | Short-term efficacy of intravitreal dobesilate in central serouschorioretinopathy 显示文摘 | Cuevas P Outeirino LA Azanza C Angulo J Gimenez-GallegoG | 2012 | Eur J Med Res2012，17，1： | 1 |
| 13 | Antiproliferative action of neomycin is associated with inhibition of cyclin D1 activation in glioma cells显示文摘 | Cuevas P Diaz-Gonzalez D Dujovny M | 2003 | Neural Res2003，25，7： | 1 |
| 14 | Fibroblast growth factor and cerebral edema显示文摘 | Cuevas P Carceller F Gimenez GG | 1994 | Neurol Res1994，16，3： | 1 |
| 15 | Melting the secrets of gelatinisation temperature in rice 显示文摘 | CUEVAS R P DAYGON V D CORPUZ H M | 2010 | Functional Plant Biology2010，37，5： | 1 |
| 16 | Asymptotic periodicity for some evolution equations in Banach spaces显示文摘 | Agarwal R P Cuevas Claudio Soto H El-Gebeily M | | 0，，05： | 1 |
| 17 | Existence results for a fractional equation with state-dependent delay显示文摘 | Dos Santos J P C Cuevas C De Andrade B | | 0，，： | 1 |
| 18 | Using next-generation sequencing approaches to isolate simple sequence repeat (SSR) loci in the plant sci- ences显示文摘 | Zalapa JE Cuevas H Zhu HY Steffan S Senalik D Zeldin E McCown B Harbut R Simon P | 2012 | Am J Bet2012，99，2： | 1 |
| 19 | Serum levels of basic fibyroblast growth factor in acute myocardial infarction 显示文摘 | Barrios V Gimenez-Gallego G | 1997 | Eur J Med Res1997，2，： | 1 |
| 20 | Fibroblast growth factors,proteins with a broad spectrum of biological activities显示文摘 | Giménez-Gallego G Cuevas P | 1994 | Neurol Res1994，16，4： | 1 |
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