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| 1 | Secreted protein acidic and rich in cysteine(SPARC)induces epithelial-mesenchymal transition,enhancing migration and invasion,and is associated with high Gleason score in prostate cancer显示文摘Secreted protein acidic and rich in cysteine(SPARC)is a matricellular protein highly expressed in bone tissue that acts as achemoattractant factor promoting the arrival of prostate cancer(PCa)cells to the bone marrow.However,the contribution of SPARCduring the early stages of tumor progression remains unclear.In this study,we show that SPARC is highly expressed in PCa tissueswith a higher Gleason score.Through stable knockdown and overexpression of SPARC in PC3 and LNCaP cells,respectively,here wedem on strate that en doge nous SPARC induces the epithelial-mesenchymal tran sition(EMT),decreasing E-cadheri n and cytokeratin18 and increasing N-cadheri n and vime ntin.Moreover,SPARC in duces the expression of EMT regulatory tran scription factors Snailfamily transcriptional repressor 1(Snail),Snail family transcriptional repressor 2(Slug),and zinc finger E-box binding homeobox 1(Zeb1).In addition,SPARC knockdown in PC3 cells decreases migration and invasion in vitro,without modifying cell proliferation.Our results indicate that SPARC might facilitate tumor progression by modifying the cellular phenotype in cancer cells. | Fernanda Lopez-Moncada Maria Jose Torres Enrique A Castellon Hector R Contreras | 2019 | Asian Journal of Andrology2019，21，6： | 5 |
| 2 | The transcription factor ZEB1 promotes chemoresistance in prostate cancer cell lines显示文摘One of the factors promoting tumoral progress is the abnormal activation of the epithelial-mesenchymal transition(EMT)program which has been associated with chemoresistance in tumoral cells.The transcription factor zinc finger E-box-binding homeobox 1(ZEB1),a key EMT activator,has recently been related to docetaxel resistance,the main chemotherapeutic used in advanced prostate cancer treatment.The mechanisms involved in this protective effect are still unclear.In a previous work,we demonstrated that ZEB1 expression induced an EMT-like phenotype in prostate cancer cell lines.In this work,we used prostate cancer cell lines 22Rvl and DU145 to study the effect of ZEB1 modulation on docetaxel resistance and its possible mechanisms.The results showed that ZEB1 overexpression conferred to 22Rvl cell resistance to docetaxel while its silencing made DU145 cells more sensitive to it.Analysis of resistance markers showed no presenee of ATP-binding cassette subfamily B member 1(MDR1)and no changes in breast cancer resistance protein(BCRP)or ATP-binding cassette subfamily C member 10(MRP7).However,a correlation between ZEB1,multidrug resistance-associated protein 1(MRP1),and ATP-binding cassette subfamily C member 4(MRP4)expression was observed.MRP4 inhibition,using MK571,resensitized cells with ZEB1 overexpression to docetaxel treatment.In addition,modulation of ZEB1 and subsequent change in MRP4 expression correlated with a lower apoptotic response to docetaxel,characterized by lower B-cell lymphoma 2(Bcl2),high BCL2-associated X protein(Bax),and high active caspase 3 expression.The response to docetaxel in our model seems to be mediated mainly by activation of the apoptotic death program.Our results showed that modulation of MRP4 could be a mediator of ZEBl-related resistance to docetaxel in prostate cancer,making it a possible marker for chemotherapy response in patients who do not express MDR1. | Octavio Orellana-Serradell Daniela Herrera Enrique A Castellon Hector R Contreras | 2019 | Asian Journal of Andrology2019，21，5： | 5 |
| 3 | The transcription factor ZEB1 promotes an aggressive phenotype in prostate cancer cell lines显示文摘 | Octavio Orellana-Serradell Daniela Herrera Enrique A Castellon Hector R Contreras | 2018 | Asian Journal of Andrology2018，20，3： | 4 |
| 4 | syndecan-1和-2表达形式的变化可以预测前列腺癌生化复发显示文摘前列腺癌的临床特点不能准确确定患者是否会发生生化复发,因而不适合作为预后和复发的指标。需要新的分子标记物对患者进行适当的术前风险分层。本研究旨在评估syndecan-1和-2的表达改变是否可以预测临床局限性前列腺癌患者行前列腺根治术后的生化复发。取60份局限性前列腺癌患者的石蜡包埋组织标本,采用免疫组化染色检测syndecan-1和-2的表达。取10份良性前列腺增生患者的样本作为非恶性对照。利用半定量分析,对其进行染色评估。为探讨预后效果,用Kaplan—Meier生存曲线进行分析,并进行log—rank检验。在良性前列腺样本中,syndecan-1在基底层上皮细胞中有表达,在上皮分泌细胞的基侧膜上也有表达;syndecan-2则主要但挞底层上皮细胞中表达。前列腺癌样本中,两种syndecan的表达模式都转变成定位在颗粒状细胞质上。生存分析显示,在游离前列腺特异性抗原(fPSA)复发存活曲线中,正常和改变的syndecan-1和-2的表达存在显著差异(P〈0.05)。这些数据表明,syndecan—1和-2可以作为临床局灶性前列腺癌患者预后的标记物,从而改进前列腺特异性抗原(PSA)评估复发风险,增加风险分层。 | Rodrigo Ledezma Federico Cifuentes Ivan Gallegos Juan Fulla Enrique Ossandon Enrique A Castellon Hector R Contreras | 2011 | Asian Journal of Andrology2011，13，3： | 2 |
| 5 | Friction-stir welding of a beryllium-aluminum powder metallurgy alloy显示文摘 | CONTRERAS F TRILLO E A MURR L E | 2002 | Journal of materials science2002，37，： | 1 |
| 6 | Simulation and Evaluation of Optimization Problem Solutions in Distributed Energy Management Systems显示文摘 | Contreras J Losi A Russo M | 2002 | IEEE Trans on Power Systems2002，17，1： | 1 |
| 7 | Diode characteristics in state-of-the-art ZnO/CdS/Cu (In1-x Gax ) Se2 solar cells显示文摘 | Contreras M A Rarnanathan K Abushama J | 2005 | Progress in Photovoltaics : Research and Applications2005，13，： | 1 |
| 8 | Gas chromatographic determination of pesticides in vegetable samples by sequential positive and negative chemical ionization and tandem mass spectrometric fragmentation using an ion trap analyzer显示文摘 | Hemando M D Aguera A Fernandez-Alba A R Piedra L Contreras M | | 0，，： | 1 |
| 9 | Mierostructural evolution during large strain hot rolling of an AM60 alloy 显示文摘 | PEREZ - PRADO M T VALLE J A CONTRERAS J M RUANOO A | 2004 | Scripta Mater2004，50，5： | 1 |
| 10 | Differential responses to copper-induced oxidative stress in the marine macroalgae Lessonia nigrescens and Scytosiphon lomentaria (Phaeophyeeae) 显示文摘 | Contreras L Mella D Moenne A | 2009 | Aquatic Toxicology2009，94，2： | 1 |
| 11 | Forecasting next-day electricity prices by time series models显示文摘 | Nogales F J Contreras J Conejo A J | 2002 | IEEE Transaction on Power Systems2002，17，2： | 1 |
| 12 | Encapsulation and growth of gold nanoparticles in thermore- sponsive microgels 显示文摘 | CONTRERAS - CACERES R SANCHEZ - LGLESIAS A KARG M | 2008 | Advanced Materials2008，20，9： | 1 |
| 13 | Evaluating tree competition indices as predictors of basal area incre- ment in western Montana forests 显示文摘 | CONTRERAS M A AFFILECK D CHUNG W | 2011 | For Ecol Manage2011，262，11： | 1 |
| 14 | TNF-αand IL-10 promoter polymorphisms,HPV infection,and cervical cancer risk显示文摘 | Barbisan G Perez LO Contreras A | 2012 | Tumour Biol2012，33，5： | 1 |
| 15 | Tight bounds from a path based formulation for the tree of hub location problem显示文摘 | Contreras I Fernández E Marín A | 2009 | Computers&Operations Research2009，36，12： | 1 |
| 16 | Incidence of Oral Mucositis, Its Treatment and Pain Management in Patients Receiving Cancer Treatment at Radiation Oncology Depart- ments in Spanish Hospitals (MUCODOL Study)显示文摘 | Manas A Palacios A Contreras J | 2009 | Clin Transl Oncol2009，11，10： | 1 |
| 17 | Microstmcture and properties of hercynite- magnesia-calcium zirconate refractory mixtures 显示文摘 | Contreras J E Castillo G A | 2005 | Materials Characterization2005，54，11： | 1 |
| 18 | Herpesviruses in periodontal pocket and gingival tissue specimens显示文摘 | Nowzari H Slots J | 2000 | Oral Microbiol Immunol JT-Oral microbiology and immunology2000，15，1： | 1 |
| 19 | Heart rate variability as early marker of multiple organ dysfunction syndrome in septic patients显示文摘 | Pontet J Contreras P Curbelo A | 2003 | J Crit Care2003，18，3： | 1 |
| 20 | Electrodeposition of In-Se, Cu-Se and Cu-In-Se thin films显示文摘 | Bhattacharya R N Fernadez A M Contreras M A | 1996 | J Electrochem Soc1996，143，： | 1 |
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