|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Mapping the immune microenvironment for mandibular alveolar bone homeostasis at single-cell resolution显示文摘Alveolar bone is the thickened ridge of jaw bone that supports teeth.It is subject to constant occlusal force and pathogens invasion,and is therefore under active bone remodeling and immunomodulation.Alveolar bone holds a distinct niche from long bone considering their different developmental origin and postnatal remodeling pattern.However,a systematic explanation of alveolar bone at single-cell level is still lacking.Here,we construct a single-cell atlas of mouse mandibular alveolar bone through single-cell RNA sequencing(scRNA-seq).A more active immune microenvironment is identified in alveolar bone,with a higher proportion of mature immune cells than in long bone.Among all immune cell populations,the monocyte/macrophage subpopulation most actively interacts with mesenchymal stem cells(MSCs)subpopulation.Alveolar bone monocytes/macrophages express a higher level of Oncostatin M(Osm)compared to long bone,which promotes osteogenic differentiation and inhibits adipogenic differentiation of MSCs.In summary,our study reveals a unique immune microenvironment of alveolar bone,which may provide a more precise immune-modulatory target for therapeutic treatment of oral diseases. | Weimin Lin Qiwen Li Danting Zhang Xiaohan Zhang Xingying Qi Qian Wang Yaqian Chen Caojie Liu Hanwen Li Shiwen Zhang Yuan Wang Bin Shao Li Zhang Quan Yuan | 2021 | Bone Research2021，9，1： | 6 |
| 2 | Functional metabolomics reveal the role of AHR/GPR35 mediated kynurenic acid gradient sensing in chemotherapy-induced intestinal damage显示文摘Intestinal toxicity induced by chemotherapeutics has become an important reason for the interruption of therapy and withdrawal of approved agents. In this study, we demonstrated that chemotherapeutics-induced intestinal damage were commonly characterized by the sharp upregulation of tryptophan(Trp)àkynurenine(KYN)àkynurenic acid(KA) axis metabolism. Mechanistically,chemotherapy-induced intestinal damage triggered the formation of an interleukin-6(IL-6)àindoleamine2,3-dioxygenase 1(IDO1)àaryl hydrocarbon receptor(AHR) positive feedback loop, which accelerated kynurenine pathway metabolism in gut. Besides, AHR and G protein-coupled receptor 35(GPR35) negative feedback regulates intestinal damage and inflammation to maintain intestinal integrity and homeostasis through gradually sensing kynurenic acid level in gut and macrophage, respectively. Moreover, based on virtual screening and biological verification, vardenafil and linagliptin as GPR35 and AHR agonists respectively were discovered from 2388 approved drugs. Importantly, the results that vardenafil and linagliptin significantly alleviated chemotherapy-induced intestinal toxicity in vivo suggests that chemotherapeutics combined with the two could be a promising therapeutic strategy for cancer patients in clinic.This work highlights GPR35 and AHR as the guardian of kynurenine pathway metabolism and core component of defense responses against intestinal damage. | Di Wang Danting Li Yuxin Zhang Jie Chen Ying Zhang Chuyao Liao Siyuan Qin Yuan Tian Zunjian Zhang Fengguo Xu | 2021 | Acta Pharmaceutica Sinica B2021，11，3： | 4 |
| 3 | Priming of NLRP3 inflammasome activation by Msn kinase MINK1 in macrophages显示文摘The nucleotide-binding domain,leucine-rich-repeat containing family,pyrin domain-containing 3(NLRP3)inflammasome is essential in inflammation and inflammatory disorders.Phosphorylation at various sites on NLRP3 differentially regulates inflammasome activation.The Ser725 phosphorylation site on NLRP3 is depicted in multiple inflammasome activation scenarios,but the importance and regulation of this site has not been clarified.The present study revealed that the phosphorylation of Ser725 was an essential step for the priming of the NLRP3 inflammasome in macrophages.We also showed that Ser725 was directly phosphorylated by misshapen(Msn)/NIK-related kinase 1(MINK1),depending on the direct interaction between MINK1 and the NLRP3 LRR domain.MINK1 deficiency reduced NLRP3 activation and suppressed inflammatory responses in mouse models of acute sepsis and peritonitis.Reactive oxygen species(ROS)upregulated the kinase activity of MINK1 and subsequently promoted inflammasome priming via NLRP3 Ser725 phosphorylation.Eliminating ROS suppressed NLRP3 activation and reduced sepsis and peritonitis symptoms in a MINK1-dependent manner.Altogether,our study reveals a direct regulation of the NLRP3 inflammasome by Msn family kinase MINK1 and suggests that modulation of MINK1 activity is a potential intervention strategy for inflammasomerelated diseases. | Kaixiang Zhu Xuexiao Jin Zhexu Chi Sheng Chen Songquan Wu Richard D.Sloan Xuai Lin Dante Neculai Di Wang Hu Hu Linrong Lu | 2021 | Cellular & Molecular Immunology2021，18，10： | 2 |
| 4 | Root Morphology and Anatomy Affect Cadmium Translocation and Accumulation in Rice显示文摘Paddy fields contaminated with cadmium(Cd)present decreased grain yield and produce Cckcontaminated grains.Screening for low-Cd-accumulating cultivars is a useful method to reduce the amount of Cd in the grains.The present study aimed to examine the roles of the root morphology and an atomy in Cd tran slocati on and accumulati on in rice plants.Twenty・two rice cultivars were used in the first experiment,after which two cultivars[Zixiangnuo(ZXN)and Jinyou T36(JYT36)]were selected and used in subsequent experiments under hydroponic conditions.The results showed that there were significant differences in Cd concentrations in the shoots(ranging from 4 to 100 mg/kg)and the Cd translocation rates(shoot/root)(from 7%to 102%)among the 22 cultivars,and the shoot Cd concentration was significantly correlated with the Cd translocation rate of the 22 cultivars under 0.1 mg/L Cd treatment.Compared with cultivar ZXN,JYT36 had greater root Cd uptake and accumulation but lower shoot Cd accumulation and Cd translocation rate.The number of root tips per surface area of cultivar ZXN was greater than that of JYT36,while the average root diameter was lower than that of JYT36.Compared with ZXN,JYT36 had stronger apoplastic barriers,and the Casparian bands and suberin lamellae in the root endodermis and exodermis were closer to the root apex in both the control and Cd treatments,especially for suberin lamellae in the root exodermis with Cd treatments,with a differenee of 25 mm.The results also showed that,compared with ZXN,JYT36 had greater percentages of Cd bound in cell walls and intracellular Cd but lower Cd concentrations in the apoplastic fluid under the Cd treatment.The results suggested that Cd translocation,rather than root Cd uptake,is a key process that determi nes Cd accumulati on in the rice shoots.The root morphological and an atomical characteristics evidently affect Cd accumulation in the shoots by inhibiting Cd translocation,especially via the apoplastic pathway.It was possible to pre-screen low・Cd・accumulating rice cultivars on the basis of their root morphology,an atomical characteristics and Cd tran slocati on rate at the seedling stage. | XIAO Anwen CHEN Danting LI Wai Chin YE Zhihong | 2021 | Rice science2021，28，6： | 1 |
| 5 | Fine mapping of S32(t), a new gene causing hybrid embryo sac sterility in a Chinese landrace rice (Oryza sativa L.)显示文摘 | Danting Li Liangming Chen Ling Jiang Susong Zhu Zhigang Zhao Shijia Liu Ning Su Huqu Zhai Hiroshi Ikehashi Jianmin Wan | 2007 | Theoretical and Applied Genetics2007，，3： | 1 |
| 6 | Genetic dissection of top three leaf traits in rice using progenies from a japonica × indica cross显示文摘The size of the top three leaves of rice plants is strongly associated with yield; thus, it is important to consider quantitative traits representing leaf size(e.g.,length and width) when breeding novel rice varieties.It is challenging to measure such traits on a large scale in the field, and little is known about the genetic factors that determine the size of the top three leaves. In the present study, a population of recombinant inbred lines(RILs) and reciprocal single chromosomal segment substitution lines(SSSLs) derived from the progeny of a japonica Asominori ? indica IR24 cross were grown under four diverse environmental conditions. Six morphological traits associated with leaf size were measured,namely length and width of the flag, second and third leaves. In the RIL population, 49 QTLs were identified that clustered in 30 genomic region. Twenty-three of these QTLs were confirmed in the SSSL population. A comparison with previously reported genes/QTLs revealed eight novel genomic regions that contained uncharacterized ORFs associated with leaf size. The QTLs identified in this study can be used for markerassisted breeding and for fine mapping of novel genetic elements controlling leaf size in rice. | Changbin Yin Huihui Li Zhigang Zhao Zhiquan Wang Shijia Liu Liangming Chen Xi Liu Yunlu Tian Juan Ma Lidong Xu Dashuang Zhang Susong Zhu Danting Li Jianmin Wan Jiankang Wang | 2017 | Journal of Integrative Plant Biology2017，59，12： | 1 |
| 7 | OsNPF3.1,a member of the NRT1/PTR family,increases nitrogen use efficiency and biomass production in rice显示文摘The overuse of nitrogen(N)fertilizer in fields has increased production costs and raised environmental concerns.Increasing the N use efficiency(NUE)of rice varieties is crucial for sustainable agriculture.Here we report the cloning and characterization of OsNPF3.1,a gene that controls rice NUE.An amino acid mutation in the OsNPF3.1 coding region caused different NUEs in wild and cultivated rice.OsNPF3.1,which is expressed mainly in the aerial parts of rice,also affects rice plant height,heading date,and thousand-grain weight.The OsNPF3.1 protein is located in the plasma membrane.When OsNPF3.1 was subjected to artificial selection,two naturally varying loci were associated with NUE,of which OsNPF3.1Chr6_8741040differed between indica and japonica rice.OsNPF3.1 can be used as a new target gene for breeding rice varieties with high NUE. | Xinghai Yang Baoxuan Nong Can Chen Junrui Wang Xiuzhong Xia Zongqiong Zhang Yu Wei Yu Zeng Rui Feng Yanyan Wu Hui Guo Haifeng Yan Yuntao Liang Shuhui Liang Yong Yan Danting Li Guofu Deng | 2023 | The Crop Journal2023，11，1： | 0 |
| 8 | Limosilactobacillus mucosae FZJTZ26M3 prevents NAFLD in mice through modulation of lipid metabolism and gut microbiota dysbiosis显示文摘Lactobacillus are considered promising therapeutic methods for nonalcoholic fatty liver disease(NAFLD).The effects of two strains of Ltmosilactobacillus mucosae on NAFLD were investigated in this study.Fourweek-old male C57BL/6J mice were divided into 4 groups(n=8 per group,Control,Model,FZJTZ26M3,FGSYC17L3).L.mucosae FZJTZ26M3 reduced the mice 's body weight,liver weight,and adipose tissue weight after 12 weeks of therapy.According to serum analysis,total cholesterol,triacylglycerol,and low-density lipoprotein cholesterol significantly decreased after L.mucosae FZJTZ26M3 intervention.Liver pathology showed that L.mucosae FZJTZ26M3 was effective to ameliorate lipid deposition in NAFLD mice.Additionally,the expression of the gene related to lipid metabolism in the liver and adipose tissue was analyzed,and the results indicated that L.mucosae FZJTZ26M3 could alleviate NAFLD by regulating lipid metabolism.Furthermore,the results of 16S rRNA gene sequencing revealed a drop in the relative abundance of Ruminococcaceae,which is linked to inflammation,but the relative abundance of a potential probiotic Akkermansia significantly increased after L.mucosae FZJTZ26M3 intervention.Generally,L.mucosae FZJTZ26M3 could be a candidate to prevent NAFLD. | Danting Dang Bowen Li Mengfan Ding RPaul Ross Catherine Stanton Jianxin Zhao Bo Yang Wei Chen | 2024 | Food Science and Human Wellness2024，13，3： | 0 |
| 9 | ORP8 acts as a lipophagy receptor to mediate lipid droplet turnover显示文摘Lipophagy,the selective engulfment of lipid droplets(LDs)by autophagosomes for lysosomal degradation,is critical to lipid and energy homeostasis.Here we show that the lipid transfer protein ORP8 is located on LDs and mediates the encapsulation of LDs by autophagosomal membranes.This function of oRP8 is independent of its lipid transporter activity and is achieved through direct interaction with phagophore-anchored LC3/GABARAPs.Upon lipophagy induction,ORP8 has increased localization on LDs and is phosphorylated by AMPK,thereby enhancing its affinity for LC3/GABARAPs.Deletion of ORP8 or interruption of ORP8-LC3/GABARAP interaction results in accumulation of LDs and increased intracellular triglyceride.Overexpression of ORP8 alleviates LD and triglyceride deposition in the liver of ob/ob mice,and Osbpl8 mice exhibit liver lipid clearance defects.Our results suggest that ORP8 is a lipophagy receptor that plays a key role in cellular lipid metabolism. | Maomao Pui Wenhui Zheng Hongtao Zhang Wei Wan Chao Peng Xuebo Chen Xinchang Liu Zizhen Xu Tianhua Zhoul Qiming Sun Dante Neculai Wei Liu | 2023 | Protein & Cell2023，14，9： | 0 |
| 10 | Transcriptome analysis reveals potential genes associated with plant height in rice显示文摘Plant height(PH)is a complex trait regulated by the environment and multiple genes.PH directly affects crop yield,harvest index,and lodging resistance.From plant dwarf mutants,many genes related to PH have been identified and described.Nonetheless,the molecular mechanism of height regulation in high-culm rice mutants has not been well studied.By using transcriptome and weighted gene co-expression network analysis(WGCNA),we identified the differentially expressed genes(DEGs)between high-culm rice mutants(MUT)and wild-type(WT)and explored the key pathways and potential candidate genes involved in PH regulation.Transcriptome analysis identified a total of 2,184 DEGs,of which 1,317 were identified at the jointing stage and 1,512 were identified at the heading stage.Kyoto Encyclopedia of Genes and Genomes enrichment showed that the enrichment pathways were mainly involved in plant hormone signal transduction,ABC transportation,and steroid hormone biosynthesis.Among these metabolic pathways,LOC_Os05g43910 and LOC_Os01g35030 were auxin(IAA)-related genes,up-regulated in MUT and LOC_Os02g08500(LEPTO1),LOC_Os11g04720,and LOC_Os12g04500 were cytokinin(CK)-related genes,downregulated in MUT.The WGCNA identified four modules(light cyan,dark grey,grey,and pale turquoise)closely related to PH,and seven key genes were screened from these modules,of which two were up-regulated cell wallrelated genes(LOC_Os01g26174(OsWAK5),LOC_Os06g05050)in MUT,and one gibberellic acid(GA)gene(LOC_Os06g37364,OsKO2)was also up-regulated.These genes might be closely related to PH regulation.These findings help us better understand the transcriptional regulation of rice plant growth and development and provide a theoretical basis for mapping and cloning the PH regulatory genes. | CAN CHEN RUI FENG ZONGQIONG ZHANG XIUZHONG XIA BAOXUAN NONG YU ZENG HUI GUO XINGHAI YANG DANTING LI | 2023 | BIOCELL2023，47，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员