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| 1 | Diagnosis of intestinal tuberculosis using a monoclonal antibody to Mycobacterium tuberculosis显示文摘AIM:To investigate the utility of immunohistochemical(IHC) staining with an antibody to Mycobacterium tuberculosis(M.tuberculosis) for the diagnosis of intestinal tuberculosis(TB).METHODS:We retrospectively identified 10 patients(4 males and 6 females;mean age = 65.1 ± 13.6 years) with intestinal TB.Clinical characteristics,including age,gender,underlying disease,and symptoms were obtained.Chest radiograph and laboratory tests,including sputum Ziehl-Neelsen(ZN) staining,M.tuberculosis culture,and sputum polymerase chain reaction(PCR) for tubercle bacilli DNA,as well as Tuberculin skin test(TST) and QuantiFERON-TB gold test(QFT),were examined.Colonoscopic records recorded on the basis of Sato's classification were also reviewed,in addition to data from intestinal biopsies examined for histopathological findings,including hematoxylin and eosin staining,and ZN staining,as well as M.tuberculosis culture,and PCR for tubercle bacilli DNA.For the present study,archived formalin-fixed paraffin-embedded(FFPE) intestinal tissue samples were immunohistochemically stained using a commercially available species-specific monoclonal antibody to the 38-kDa antigen of the M.tuberculosis complex.These sections were also stained with the pan-macrophage marker CD68 antibody.RESULTS:From the clinical data,we found that no patients were immunocompromised,and that the main symptoms were diarrhea and weight loss.Three patients displayed active pulmonary TB,six patients(60%) had a positive TST,and 4 patients(40%) had a positive QFT.Colonoscopic findings revealed that all patients had type 1 findings(linear ulcers in a circumferential arrangement or linear ulcers arranged circumferentially with mucosa showing multiple nodules),all of which were located in the right hemicolon and/or terminal ileum.Seven patients(70%) had concomitant healed lesions in the ileocecal area.No acid-fast bacilli were detected with ZN staining of the intestinal tissue samples,and both M.tuberculosis culture and PCR for tubercle bacilli DNA were negative in all samples.The histopathological data revealed that tuberculous granulomas were present in 4 cases(40%).IHC staining in archived FFPE samples with anti-M.tuberculosis monoclonal antibody revealed positive findings in 4 patients(40%);the same patients in which granulomas were detected by hematoxylin and eosin staining.M.tuberculosis antigens were found to be mostly intracellular,granular in pattern,and primarily located in the CD68 + macrophages of the granulomas.CONCLUSION:IHC staining with a monoclonal antibody to M.tuberculosis may be an efficient and simple diagnostic tool in addition to classic examination methods for the diagnosis of intestinal TB. | Yasushi Ihama Akira Hokama Kenji Hibiya Kazuto Kishimoto Manabu Nakamoto Tetsuo Hirata Nagisa Kinjo Haley L Cash Futoshi Higa Masao Tateyama Fukunori Kinjo Jiro Fujita | 2012 | World Journal of Gastroenterology2012，18，47： | 12 |
| 2 | Optical character recognition by the method of moments显示文摘 | Cash G L Hatamian M | 1987 | Computer Vision Graphics and Image Processing1987，39，： | 1 |
| 3 | Proteomics:a new approach to the study of disease显示文摘 | Chambers G Lawrie L Cash P | 2000 | The Journal of Pathology2000，192，： | 1 |
| 4 | The great American shape-up:Body image survey report显示文摘 | Cash T F Winstead B A Janda L H | 1986 | Psychol Today1986，20，4： | 1 |
| 5 | Proteomics: a new approach to the study of disease显示文摘 | Chambers G Lawrie L Cash P | 2000 | J Pathol2000，192，3： | 1 |
| 6 | Proteomics: a new approach to the study of disease显示文摘 | Chambers G Lawrie L Cash P | 2000 | J Pathol2000，192，3： | 1 |
| 7 | Transmission of MPEG - 2 streams over non - guaranteed quality of service networks 显示文摘 | BASSO A CASH G L CIVANLAR M R | 1997 | Picture Coding Symposium1997，，4： | 1 |
| 8 | Protein phosphatase 5 mediates lipid metabolism through reciprocal con- trol of glucocorticoid receptor and peroxisome proliferator-acti- rated receptor-γ (PPARγ) 显示文摘 | Hinds T D Jr Stechschulte L A Cash H A | 2011 | J Biol Chem2011，286，50： | 1 |
| 9 | Mechanism for the desulfurization of L-cysteine catalyzed by the nifS gene product 显示文摘 | Zheng L White R H Cash V L | 1994 | Biochemistry1994，33，15： | 1 |
| 10 | Rocky Mountain leather', sepiolite and attapulgite-an infrared emission spectroscopic study显示文摘 | Frost R L Cash G A Kloprogge J T | 1998 | Vib Speetrose1998，16，： | 1 |
| 11 | Results of 2-year follow-up of a randomized,double-blind,controlled trial of flu oroscopic candalepidural injections in central spinal stenosis显示文摘 | Manchikanti L Cash KA McManus CD | 2012 | Pain Physician2012，15，5： | 1 |
| 12 | Proteomics: a new approach to the study of disease 显示文摘 | Chambers G Lawrie L Cash P | 2000 | J Pathol2000，192，3： | 1 |
| 13 | ‘ Rocky Mountain leather', sepiolite and attapulgite--an infrared emission spectroscopic study显示文摘 | Ray L Frost Greg A Cash J Theo Kloprogge | 1998 | Vibrational Spectrosc1998，16，2： | 1 |
| 14 | Age-related prevalence of facet-joint involvement in chronic neck and low back pain显示文摘 | Manchikanti L Manchikanti KN Cash KA | 2008 | Pain Physician2008，11，1： | 1 |
| 15 | Proteomics: a new approach to the study of disease显示文摘 | Chambers G Lawrie L Cash P | 2000 | J Patho12000，192，3： | 1 |
| 16 | The preliminary results of a comparative effectiveness evaluation of adhesiolysis and caudal epidural injections in managing chronic low back pain secondary to spinal stenosis:a randomized,equivalence con- trolled trial显示文摘 | Manchikanti L Cash KA McManus CD | 2009 | Pain Physician2009，12，6： | 1 |
| 17 | Transforaminal epidural injections in chronic lumbar disc herniation:a randomized, dou- ble-blind, active-control trial 显示文摘 | Manchikanti L Cash KA Pampati V | 2014 | Pain Physician2014，17，4： | 1 |
| 18 | Fluoroscopic lumbar inter-laminar epidural injections in managing chronic lumbar axial or disco-genic pain显示文摘 | Manchikanti L Cash KA McManus CD | 2012 | J Pain Res2012，5，： | 1 |
| 19 | Proteomics:a new approach to the study of disease显示文摘 | CHAMBERS G LAWRIE L CASH P | | 0，，03： | 1 |
| 20 | Hypoxia-induced energy stressregulates mRNA translation and cell growth显示文摘 | Liu L Cash TP Jones RG | | 0，，04： | 1 |
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