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| 1 | Thiopurine-methyltransferase variants in inflammatory bowel disease:Prevalence and toxicity in Brazilian patients显示文摘AIM:To analyze the prevalence of thiopurine-methyltransferase(TPMT)genotypes and their associationwith drug toxicity in inflammatory bowel disease(IBD)patients from southeastern Brazil.METHODS:A total of 219 consecutive patients with IBD,of which 146 had Crohn’s disease and 73 had ulcerative colitis,regularly seen at the outpatient unit of the Division of Gastroenterology at the University Hospital Pedro Ernesto of the State University of Rio de Janeiro,a tertiary referral center,were enrolled in this study from February 2009 to January 2011.We analyzed the presence of major TPMT genetic variants(TPMT*2,*3A,*3C)in IBD patients by means of a specific allele and RFLP-PCR.Genomic DNA was isolated from peripheral blood leukocytes by proteinase-K/Sodium Dodecyl Sulfate digestion and phenol-chloroform extraction.TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes were detected by real-time polymerase chain reaction followed by direct sequencing with specific primers.Clinical data were systematically recorded,and correlated with the genotype results.RESULTS:The distribution of the selected TPMT gene polymorphism TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes was 3.6%,5.4%,and 7.7%of the patients,respectively.Among the side effects recorded from patients taking azathioprine,14 patients presented with pancreatitis and/or an elevation of pancreatic enzymes,while 6 patients had liver toxicity,and 2 patients exhibited myelosuppression/neutropenia.TPMT polymorphisms were detected in 37/219 patients(8 heterozygous for*2,11 heterozygous for*3A,and 18 heterozygous for*3C).No homozygotic polymorphisms were found.Despite the prevalence of the TPMT*3C genotype,no differences among the genotype frequencies were significant.Although no association was detected regarding myelotoxicity or hepatotoxicity,a trend towards the elevation of pancreatic enzymes was observed for TPMT*2 and TPMT*3C genotypes.CONCLUSION:The prevalence of TPMT genotypes was high among Brazilian patients.Variants genes*2and*3C may be associated with azathioprine pancreatic toxicity in a IBD southeastern Brazilian population. | Ana Teresa P Carvalho Barbara C Esberard Renata S B Fróes Davy C M Rapozo Ana B Grinman Tatiana A Simo Juliana C V C Santos Antonio José V Carneiro Luis Felipe Ribeiro-Pinto Heitor S P de Souza | 2014 | World Journal of Gastroenterology2014，20，12： | 3 |
| 2 | 日粮中添加氨基酸降低粗蛋白含量对6~15kg仔猪生产性能的影响显示文摘为评价添加氨基酸降低粗蛋白(CP)含量对6~15 kg仔猪的影响,共设计两个试验。在性能试验中(试验Ⅰ),21日龄、初始体重为5.95±0.33 kg的断奶仔猪120头被分成5组。试验为完全随机区组试验设计,共8个重复,每个重复3只猪。在5个处理组中,CP含量由21.0%降低到15.0%(分别为21.0%,19.5%,18.0%,16.5%和15.0%),通过添加L-赖氨酸、DL-蛋氨酸、L-苏氨酸、L-色氨酸、L-缬氨酸和L-异亮氨酸来满足日粮中氨基酸的需求。各处理间生产性能无显著差异。因此,以上蛋白质的任何水平都能被有效的合成氨基酸。降低蛋白质的含量可以增加必需氨基酸和非必需氨基酸的比值,且蛋白质含量为15%时比值最佳(5347)。随着蛋白质含量的降低,尿素含量线性下降(试验Ⅰ)。为评估氮平衡(试验Ⅱ),21日龄的杂交阉割商品仔猪20只被随机分成5组,仔猪的平均活重为10.79±2.19kg。5个处理组的所有动物安置在铁笼中,每个处理4个重复,每个重复1只猪。结果血液和尿液中尿素含量随蛋白质含量的减少线性降低(P〈0.05),进而减少了动物向环境中的氮排泄。 | Toledo J B Furlan A C Pozza P C Pianob L M Carvalho P L O Peuela-Sierra L M Huepa L M D | 2014 | 饲料博览2014，0，11： | 3 |
| 3 | Recovery of 2-chlorohenol from aqueous solutions by emulsion liquid membrane: batch experimental studies and modeling显示文摘 | Correia P F M M Carvalho J M R | 2000 | J Membr Sci2000，179，12： | 2 |
| 4 | An inverse scattering series method for attenuating multiples in seismic reflection data显示文摘 | Weglein A B Gasparotto F A Carvalho P M Stolt R H | 1997 | Geophysics1997，62，： | 1 |
| 5 | In-line Sagnac interferometer current sensor显示文摘 | BLAKE J TANTASWADI P de CARVALHO R T | 1996 | IEEE Transaction on Power Delivery1996，11，1： | 1 |
| 6 | Ultra-performance liquid chromatographytandem mass spectrometric method for the determination of artemisinin in rat serum and its application in pharmacokinetics 显示文摘 | LI L D Pabbisetty P Carvalho | 2008 | J Chromatogr B2008，867，： | 1 |
| 7 | An expeditiousmethod for the determ ination of organochlorine pesticides resi-dues in estuarine sediments using microwave assisted pre-ex-traction an d automa ted headspace solid phase microextractioncoupled to gas chromatography-mass spectrometry显示文摘 | Carvalho P N Rodrigues P N R Alves F | 2008 | Talan-ta2008，76，5： | 1 |
| 8 | Reproductive biology of crocodile shark Pseudocarcharias kamoharai 显示文摘 | OLIVEIRA P HAZIN F H V CARVALHO F | 2010 | Journal of Fish Biology2010，76，7： | 1 |
| 9 | BAC to the future or oligonucleotides:a perspective for microarray comparative genomic hybridization (array CGH)显示文摘 | Ylstra B van den Ijssel P Carvalho B | 2006 | Nucl Acid Res2006，34，： | 1 |
| 10 | Discrete wavelet transform wignal analyzer显示文摘 | Cox P H CARVALHO A A | 2007 | IEEE Transactions on Instrumentation and Measurement2007，56，5： | 1 |
| 11 | Decision criteria for the exploration of ornamental-stone deposits: Application to the marbles of the Portuguese estremoz anticline 显示文摘 | CARVALHO J F HENRIQUES P FALE P LUIS G | 2008 | International Journal of Rock Mechanics and Mining Sciences2008，45，8： | 1 |
| 12 | Advances in enhanced biological phosphorus removal: from micro to macro scale 显示文摘 | OEHMEN A LEMOS P C CARVALHO G | 2007 | Water Research2007，41，11： | 1 |
| 13 | Advances in enhanced biological phosphorus removal:from micro to macro scale显示文摘 | Oehmen A Lemos P C Carvalho G | 2007 | Water Research2007，41，11： | 1 |
| 14 | Preliminary study on the molecular weight profile of soluble protein nitrogen in live food organisms for fish larvae显示文摘 | Carvalho A P Oliva-Teles A Bergot P A | 2003 | Aquaculture2003，225，： | 1 |
| 15 | Evaluation of palm kernel meal and corn distillers grains in corn silage-based diets for lactating dairy cows显示文摘 | Carvalho L P F A R J Cabrita R J Dewhurst | 2006 | J Dairy Sci2006，89，： | 1 |
| 16 | Photocatalyst TiO2-Co: the effect of doping depth profile on methylene blue degradation显示文摘 | CARVALHO H P BATISTA A L BERTHOLDO R | 2010 | Journal of Materials Science2010，45，20： | 1 |
| 17 | Microstructure,mechanical Properties and wearresistance of high speedsteel rolls for hot rolling mills 显示文摘 | Carvalho M A De Xavier R R Filho C S P | 2002 | I& SM2002，29，1： | 1 |
| 18 | Effect of PUFA-rich supplements from marine sources in the feed of laying hens on lipid composition and the percent- age of incorporation of n-6 PUFAs in egg yolk 显示文摘 | CARVALHO P R PITA M C G PIBER N E | 2009 | Ar- quivous do Instituto Biologico2009，76，2： | 1 |
| 19 | Pressure ulcer risk factors in an ICU population显示文摘 | Jirieka MK Ryan P Carvalho MA | 1995 | Am J Crit Care1995，4，5： | 1 |
| 20 | Light requirements in microalga| photobioreactors: an overview of biophotonic aspects 显示文摘 | Carvalho A P Silva S O Baptista J M | 2011 | Applied Microbiology and Biotechnology2011，89，5： | 1 |
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