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| 1 | Liver fat deposition and mitochondrial dysfunction in morbid obesity:An approach combining metabolomics with liver imaging and histology显示文摘AIM: To explore the usefulness of magnetic resonance imaging(MRI) and spectroscopy(MRS) for assessment of non-alcoholic fat liver disease(NAFLD) as compared with liver histological and metabolomics findings. METHODS: Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesityinduced NAFLD in an observational, prospective, singlesite, cross-sectional study with a pre-set duration of 1 year. Relevant data were obtained prospectively and surrogates for inflammation, oxidative stress and lipid and glucose metabolism were obtained through standard laboratory measurements. To provide reliable data from MRI and MRS, novel procedures were designed to limit sampling variability and other sources of error using a 1.5T Signa HDx scanner and protocols acquired from the 3D or 2D Fat SAT FIESTA prescription manager. We used our previously described 1H NMRbased metabolomics assays. Data were obtained immediately before surgery and after a 12-mo period including histology of the liver and measurement of metabolites. Values from 1H NMR spectra obtained after surgery were omitted due to technical limitations.RESULTS: MRI data showed excellent correlation with the concentration of liver triglycerides, other hepatic lipid components and the histological assessment, w h i c h e xc l u d e d t h e p r e s e n c e o f n o n-a l c o h o l i c steatohepatitis(NASH). MRI was sufficient to follow up NAFLD in obese patients undergoing bariatric surgery and data suggest usefulness in other clinical situations. The information provided by MRS replicated that obtained by MRI using the-CH3 peak(0.9 ppm), the-CH2- peak(1.3 ppm, mostly triglyceride) and the-CH=CH- peak(2.2 ppm). No patient depicted NASH. After surgery all patients significantly decreased their body weight and steatosis was virtually absent even in patients with previous severe disease. Improvement was also observed in the serum concentrations of selected variables. The most relevant findings using metabolomics indicate increased levels of triglyceride and monounsaturated fatty acids in severe steatosis but those results were accompanied by a significant depletion of diglycerides, polyunsaturated fatty acids, glucose-6-phosphate and the ATP/AMP ratio. Combined data indicated the coordinated action on mitochondrial fat oxidation and glucose transport activity and may support the consideration of NAFLD as a likely mitochondrial disease. This concept may helpto explain the dissociation between excess lipid storage in adipose tissue and NAFLD and may direct the search for plasma biomarkers and novel therapeutic strategies. A limitation of our study is that data were obtained in a relatively low number of patients.CONCLUSION: MRI is sufficient to stage NAFLD in obese patients and to assess the improvement after bariatric surgery. Other data were superfluous for this purpose. | Nahum Calvo Raúl Beltrán-Debón Esther Rodríguez-Gallego Anna Hernández-Aguilera Maria Guirro Roger Mariné-Casadó Lidón Millá Josep M Alegret Fàtima Sabench Daniel del Castillo María Vinaixa Miguelàngel Rodríguez Xavier Correig Roberto García-álvarez Javier A Menendez Jordi Camps Jorge Joven | 2015 | World Journal of Gastroenterology2015，21，24： | 2 |
| 2 | Preparation of ω-Bromoalkan-1-ols fromα,ω-alkanediols from the continuou sex traction method显示文摘 | Camps F Casamor JM Coll J | | 0，，15： | 1 |
| 3 | High performance Nd2Fe14B magnets 显示文摘 | Kim A S Camp F E | 1996 | J Appl Phys1996，79，08： | 1 |
| 4 | Vascular endothelial growth factor receptor-1 activation mediates epithelial to mesenchymal transition in human pancreatic carcinoma cells显示文摘 | YANG AD CAMP ER FAN F | 2006 | Cancer Res2006，66，1： | 1 |
| 5 | Multiple-polymorphism associations of7matrix metalloproteinase and tissue inhibitor metalloproteinase genes with myocardial infarction and angiographic coronary artery disease显示文摘 | Horne B D Camp N J Carlquist J F | 2007 | Am Heart J2007，154，4： | 1 |
| 6 | Hydrogen induced corrosion mechanism in NdFeB magnets显示文摘 | Kim A S Camp F E Lizzi T | 1996 | Journal of Applied Physics1996，79，8： | 1 |
| 7 | Necrotizing enterocolitis in 20, 822 infants: analysis of medical and surgical treatments 显示文摘 | Abdullah F Zhang Y Camp M | 2010 | Clin Pediatr2010，49，2： | 1 |
| 8 | Specific T helper cell requirement for optimal induction of cytotoxic T lymphocytes against major histocompatibility complex class Ⅱ negative tumors 显示文摘 | Ossendorp F Mengede E Camps M | 1998 | J Exp Meal1998，187，： | 1 |
| 9 | Role of oxygen for improving magnetic properties and thermal stability of sintered(Nd,Dy)(Fe,Co)B magnets显示文摘 | Kim A S Camp F E | 1995 | IEEE Transactions on Magnetics1995，31，6： | 1 |
| 10 | Bone mineral density in ambulant, non-steroid treated female patients with rheumatoid arthritis显示文摘 | Eggelmeijer F Camps JA Valkema R | 1993 | Clin Exp Rheumatol1993，11，： | 1 |
| 11 | Vascular endothelial growth factor receptor - 1 activation mediates epithelial to mesenchymal transition in human pancreatic carcinoma cells 显示文摘 | Yang AD Camp ER Fan F | 2006 | Cancer Res2006，66，1： | 1 |
| 12 | A synthesis of levetiraeetam based on (S)-N-phenylpantolaetarn as a ehiral auxiliary显示文摘 | BOSCHI F CAMPS P FRANCHINI C F | 2005 | Tetrahedron Asymmetry2005，16，22： | 1 |
| 13 | Splenectomy in hereditary spherocytosis:review of 1,657 patients and application of the pediatric quality indicators显示文摘 | Abdullah F Zhang Y Camp M | 2009 | Pediatr Blood Cancer2009，52，： | 1 |
| 14 | Multiple poly morphis mass ociations of 7 matrix metalloproteinase and tissue inhibitor metalloproteinasegenes with myocardial infarction and anglo graphic coronary artery disease显示文摘 | Home B D Camp N J Carlquist J F | 2007 | American Heart Journal2007，154，4： | 1 |
| 15 | Effect ofoxygen on the anaerobic methanotroph ‘ CandidatusMethylomirabilis oxyfera,: kinetic and transcriptional analysis 显示文摘 | LUESKEN F A WU M L OP DEN CAMP H J M | 2012 | Environmental Microbiology2012，14，4： | 1 |
| 16 | The correlation of visibility noise and its impact on the radiometric resolution of an aperture synthesis radiometer显示文摘 | Bara J Camps A Torres F | 2000 | IEEE Trans Geosci Remote Sens2000，38，5： | 1 |
| 17 | On - board phase and modulus calibration of large aperture synthesis radiometers: study applied to MIRAS 显示文摘 | Torres F Camps A Bara J | 1996 | IEEE Transactions on Geoscience and Remote Sensing1996，34，4： | 1 |
| 18 | Cloning, Sequencing, and Functional Analysis of a Marek's Disease Virus Origin of DNA Replication显示文摘 | Camp H S Coussens P M Silva R F | 1991 | J Virol1991，65，： | 1 |
| 19 | Vascular endothelial growth factor receptor-1 activation mediates epithelial to mesenchymal transition in human pancreatic carcinoma cells显示文摘 | Yang AD Camp ER Fan F | 2009 | Cancer Res2009，66，1： | 1 |
| 20 | Synthesis of dienic fluorinated analogs of insect sex pheromone显示文摘 | CAMPS F COLL J FABRIAS G | 1984 | Tetrahedron1984，40，： | 1 |
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