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| 1 | m^6A mRNA methylation sustains Treg suppressive functions显示文摘 | Jiyu Tong Guangchao Cao Ting Zhang Esen Sefik Maria Carolina Amezcua Vesely James P Broughton Shu Zhu Huabin Li Bin Li Lei Chen Howard Y Chang Bing Su Richard A Flavell Hua-Bing Li | 2018 | Cell Research2018，28，2： | 30 |
| 2 | Highly efficient and genotype-independent barley gene editing based on anther culture显示文摘Recalcitrance to tissue culture and genetic transformation is the major bottleneck for gene manipulation in crops.In barley,immature embryos of Golden Promise have typically been used as explants for transformation.However,the genotype dependence of this approach limits the genetic modification of commercial varieties.Here,we developed an anther culture-based system that permits the effective creation of transgenic and gene-edited plants from commercial barley varieties.The protocol was tested in Golden Promise and four Australian varieties,which differed in phenology,callus induction,and green plant regeneration responses.Agrobacterium-mediated transformation was performed on microspore-derived callus to target the HvPDS gene,and T0 albinos with targeted mutations were successfully obtained from commercial varieties.Further editing of three targets was achieved with an average mutation rate of 53%in the five varieties.In 51 analyzed T0 individuals,Cas9 induced a large proportion(69%)of single-base indels and two-base deletions in the target sites,with variable mutation rates among targets and varieties.Both ontarget and off-target activities were detected in T1 progenies.Compared with immature embryo protocols,this genotype-independent platformcan deliver a high editing efficiency and more regenerant plants within a similar time frame.It shows promise for functional genomics and the application of CRISPR technologies for the precise improvement of commercial varieties. | Yong Han Sue Broughton Li Liu Xiao-Qi Zhang Jianbin Zeng Xiaoyan He Chengdao Li | 2021 | Plant Communications2021，2，2： | 3 |
| 3 | Diminished lung function, RSV infection, and respiratory morbidity in prematurely born infants 显示文摘 | Broughton S Bhat R Roberts A | 2006 | Arch Dis Child2006，91，1： | 1 |
| 4 | Conjugal transfer of cosmid DNA from Escherichia coli to Saccharopolyspora spinosa:e-ects of chromosomal insertions on A83543 macrolide production显示文摘 | Matsushima P Broughton MC Turner JR | 1994 | Gene1994，146，： | 1 |
| 5 | Nanocapillarity in fullerene tubules显示文摘 | Pederson M R Broughton J Q | 1992 | Phys Rex Lett1992，69，： | 1 |
| 6 | Immunological abnormalities in humans chronically exposed to chlorpyrifos显示文摘 | Thrasher JD Heuser G Broughton A | 2002 | Arch Environ Health2002，57，3： | 1 |
| 7 | A paradigm for the scheduling of a continuous walking beam reheat furnace u-sing a modified genetic algorithm显示文摘 | Broughton J S Mahfouf M Linkens D A | 2007 | Materials and Manufacturing Processes2007，22，5： | 1 |
| 8 | Concurrent Coupling of Length Scales: Methodology and Application显示文摘 | Broughton J Q Abraham F F Bernstei N | 1999 | Phys Rev1999，60，： | 1 |
| 9 | Immunological abnormalities in humans exposed to chlorpyrifos: preliminary observations显示文摘 | ThrasherJD Madison R Broughton A | 1993 | Arch Environ Health1993，48，2： | 1 |
| 10 | The Characterization of Brucella Strains Isolated from Marine Mammals显示文摘 | Jahans KL Foster G Broughton ES | 1997 | Vet Microbiol1997，57，： | 1 |
| 11 | Polieies and practices for aquaeuhure food safety in China显示文摘 | Broughton E I Walker D G | 2010 | Food Policy2010，35，5： | 1 |
| 12 | Enhanced biological phosphorus removal for high-strength wastewater with a low rbCOD:P ratio显示文摘 | Broughton A Pratt S Shilton A | | 0，，05： | 1 |
| 13 | Crystallization and pre-liminary X - ray diffraction analysis of the interleukin - 3 alphareceptor bound to the Fabfragment of antibody CSL362 显示文摘 | Broughton SE Hercus TR Nero TL | 2014 | ActaCrystallogr F Struct Biol Commun2014，70，3： | 1 |
| 14 | Antimicrobial Fibers Created via Polycarboxylic Acid Durable Press Finishing 显示文摘 | Lee J Broughton R M Akdag A | 2007 | Textile Research Journal2007，77，8： | 1 |
| 15 | Macrocyclic peptidomimetic inhibitors of fl-secretase ( BACE ) : First X-ray structure of a macrocyclic peptidomimetic-BACE complex 显示文摘 | ROJO I MARTIN J A BROUGHTON H | 2006 | Bioorg Med Chem Lett2006，16，1： | 1 |
| 16 | Mutations in the xeroderma pigmentosum group D DNA repair gene in patients with trichothiodystrophy显示文摘 | Broughton | 1994 | Nat Genet1994，7，： | 1 |
| 17 | Dynamic fracture of silicon: concurrent simulation of quantum electrons, classical atoms, and the continuum solid 显示文摘 | Abraham F F Bernstein N Broughton J Q | 2000 | MRS Bulletin2000，，5： | 1 |
| 18 | The Determination of Yield Strength From Hardness Measurements 显示文摘 | Cahoon J R Broughton W H Kutzak A R | 1971 | Metallurgical Transactions1971，2，7： | 1 |
| 19 | The basic science of wound healing 显示文摘 | Broughton G Ⅱ Janis JE Attinge CE | 2006 | Plast Reconstr Surg2006，117，7： | 1 |
| 20 | Bipolar hemiarthroplasty for subcapital fracture of the femoral neck显示文摘 | Emery RJ Broughton NS Desai K | | 0，，： | 1 |
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