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| 1 | Identification and mapping of quantitative trait loci controlling cold-tolerance of Chinese common wild rice (O. rufipogon Griff.) at booting to flowering stages显示文摘An advanced backcross population of rice was used to identify the quantitative trait locus (QTL) controlling the cold-tolerance at booting to flowering stages. The recipient, Guichao 2 (GC2), was a commercial Indica rice; the donor Dongxiang common wild rice, was an accession of common wild rice (DXCWR, Oryza rufipogon Griff.). Three QTLs for cold-tolerance were detected on chromosomes 1, 6 and 11. Two of them coming from DXCWR could enhance the cold-tolerance of the backcross progenies. Moreover, one sterility QTL that could reduce the seed set rate of the backcross progenies by 78% was mapped on chromosome 5. | LIU Fengxia, SUN Chuanqing, TAN Lubin, FU Yongcai, LI Dejun & WANG Xiangkun Department of Plant Genetics and Breeding, China Agricultural University, National Key Laboratory of Agrobiology, Key Laboratory of Crop Genetic Improvement and Genome of the Ministry of Agriculture, Beijing 100094, China | 2003 | Chinese Science Bulletin2003，48，19： | 29 |
| 2 | Identification of quantitative trait loci associated with salt tolerance at seedling stage from Oryza rufipogon显示文摘土壤咸度是影响工厂生长和庄稼生产的主要不能生活的压力之一。在现在的学习,在米饭幼苗舞台的盐忍耐用 87 根基因渗入线(IL ) 被评估,它从在精英 indica 栽培变种 Teqing 和普通野米饭(Oryza rufipogon Griff ) 的就职之间的一个十字被导出。实质的变化为包括盐忍耐 20 的四个特点(圣) 被观察,相对的根干燥重量(RRW ) ,干燥重量(RSW ) 和相对总数弄干的相对射击重量(RTW ) 。圣断然显著地所有另外的三个特点地被相关。与这四个特点联系的 15 通常认为的量的特点 loci (QTL ) 的一个总数用单个点的分析被检测,它位于染色体 1, 2, 3, 6, 7, 9 和 10 与解释 phenotypic 变化的 8%26% 。O。在 13 QTL (86.7%) 的导出 rufipogon 的等位基因能在 Teqing 背景改进盐忍耐。影响 RRW, RSW 和 RTW 的四 QTL 簇在染色体上被发现 6, 7, 9 和 10 分别地。在这四 QTL 簇之中,包括三 QTL (qRRW10, qRSW10 和 qRTW10 ) 的主要的簇在染色体 10 的长手臂上在制造者 RM271 附近被发现,并且 O。分别地,当 phenotypic 变化由这解释为三个特点的三单个 QTL 从 19% ~ 26% 变化了时,在这三 loci 的导出 rufipogon 的等位基因与 22.7% , 17.3% 和 18.5% 的添加剂效果增加了 RRW, RSW 和 RTW。另外,几腌容忍的 IL 被选择并且能被用于识别并且利用有利的盐从普通野米饭的容忍的基因并且在盐使用容忍的米饭繁殖编程序。 | Lei Tian,Lubin Tan,Fengxia Liu,Hongwei Cai,Chuanqing Sun State Key Laboratory of Plant Physiology and Biochemistry,National Centre for Evaluation of Agricultural Wild Plant(Rice), Laboratory of Crop Heterosis and Utilization of Ministry of Education Beijing Key Laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture,Department of Plant Genetics and Breeding,China Agricultural University,Beijing 100193,China | 2011 | Journal of Genetics and Genomics2011，38，12： | 26 |
| 3 | Efficient production of omega-3 fatty acid desaturase (sFat-1)-transgenic pigs by somatic cell nuclear transfer显示文摘Omega-3(ω-3) fatty acid desaturase transgenic pigs may improve carcass fatty acid composition. The use of transgenic pigs is also an excellent large animal model for studying the role of ω-3 fatty acids in the prevention and treatment of coronary heart disease and cancer. Transgenic pigs carrying synthesized fatty acid desaturase-1 gene (sFat-1) from Caenorhabditis briggsae by somatic cell nuclear transfer (SCNT) were produced for the first time in China. Porcine fetal fibroblast cells were transfected with a sFat-1 expression cassette by the liposome-mediated method. Transgenic embryos were reconstructed by nuclear transfer of positive cells into enucleated in vitro matured oocytes. A total of 1889 reconstructed embryos were transferred into 10 naturally cycling gilts. Nine early pregnancies were established, 7 of which went to term. Twenty-one piglets were born. The cloning efficiency was 1.1% (born piglets/transferred embryos). The integration of the sFat-1 gene was confirmed in 15 live cloned piglets by PCR and Southern blot except for 2 piglets. Expression of the sFat-1 gene in 12 of 13 piglets was detected with RT-PCR. The data demonstrates that an efficient system for sFat-1 transgenic cloned pigs was developed, which led to the successful production of piglets expressing the sFat-1 gene. | PAN DengKe1, ZHANG Li1, ZHOU YanRong2, FENG Chong1, LONG Chuan1, LIU Xiao1, WAN Rong3, ZHANG Jian3, LIN AiXing3, DONG EnQiu4, WANG ShuChen4, XU HouGang4 & CHEN HongXing2 1 Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China 2 Beijing Institute of Biotechnology, Beijing 100071, China 3 China Agricultural University, Beijing 100193, China 4 Yutian Breeding Swine Farms, Yutian 064104, China | 2010 | Science China(Life Sciences)2010，53，4： | 21 |
| 4 | Mapping of Fertility Restoring Gene for Aegilops kotschyi Cytoplasmic Male Sterility in Wheat Using SSR Markers显示文摘LK783 was found to be a good fertility restorer for K-type male sterility of wheat. Microsatellite markers were employed to map the major restoring gene in LK783. Maintainer and restorer DNA pools were established using the extreme sterile and fertile plants among (KJ5418A//911289/LK783)F1 population,respectively. Seventy-nine sets of SSR primers were screened for polymorphism between the two pools, 6 of which were found polymorphic. Linkage analysis showed that Xgwm11, Xgwm18 , Xgwm264a and Xgwm273were linked to the restoring gene in LK783, while Xgwm11, Xgwm18 and Xgwm273 were co-segregated. The distance between the Rf gene in LK783 and the three co-segregated markers was 6.54±4.37 cM, the distance between Rf gene and Xgwm264a was 5.71±4.10 cM. The four SSR markers were located on chromosome 1BS by amplifying the DNA of nulli-tetrasomics and ditelosomics of CS with the 4 sets of primers, indicating that the major restoring gene in LK783 was located on 1BS, but the relative location of the gene was different from Rfv1, allelism of the two genes should be further investigated. The breeding for new fertility restorer lines of K-type cytoplasmic male sterility in wheat would be facilitated by using the four polymorphic markers. | LIU Bao-shen, SUN Qi-xin, GAO Qing-rong, SUN Lan-zhen, XIE Chao-jie, LI Chuan-you, NI Zhong-fu and DOU Bing-de( Agronomy Department, Shandong Agricultural University, Taian 271018, P.R.China Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100094 , P. R . China ) | 2002 | Agricultural Sciences in China2002，1，8： | 13 |
| 5 | 用理化方法诱导草鱼(♀)×团头鲂()杂种和草鱼的三倍体、四倍体显示文摘草鱼Ctenopharyngodon idellus(Cuv.et Val.)是我国劳动人民发现的并久经饲养的一种淡水鱼类养殖对象。它有生长快,草食性等优点。但抗病力低,特别是在鱼种阶段死亡率高,一般在60%左右,严重的可达80%以上。为了解决这一生产上的问题,在文化大革命初期(1966年)我们和本所官桥试验场的工人同志一起, | “Domestic Fish”Research Group,Section of Fish Breeding,Second Laboratory,Institute of Hydrobiology,Hupei Province | 1976 | 水生生物学集刊1976，6，1： | 11 |
| 6 | Breeding Technology of eui-hybrids of Rice显示文摘Induced mutants of elongated uppermost internode (eui) were directly obtained after irradiation on maintainer (B) and restorer (R) lines of hybrid rice. CMS lines(A) with eui (referred to as eA), restorer lines with eui (referred to as eR) as well as hybrid rice with eui gene (referred to as e-hybrids)were developed. The results showed that the eui mutation frequency averaged 0.10‰ at a range of 0.02‰ - 0.47‰,varying with applied dosage and mutated materials. Genetic diversities of eui stock, and a new eui gene, temporarily designated as eui2(t), was identified. eA lines have a significantly elongated uppermost internode causing better panicle exsertion and possessing higher outcrossing potential. e-hybrids increased plant height by 3 - 10 cm and headed 2 - 3 days earlier, as well as showed greater grain weight resulting in higher yield potential than that of corresponding original hybrids. The technology systems without GA3, a few of using GA3 in hybrid rice seed production, and the establishment of e-hybrid rice breeding system were also discussed in this paper. | YANG Ren-cui, ZHANG Shu-biao, HUANG Rong-hua, YANG Shu-Ian and ZHANG Qing-qi(Institute of Genetics and Crop Breeding , Fujian Agricultural and Forestry University, Fuzhou 350002 , P. R . China ) | 2002 | Agricultural Sciences in China2002，1，4： | 11 |
| 7 | 散鳞镜鲤与兴国红鲤、龙州镜鲤的杂种优势以及鳞被、体色的遗传显示文摘本研究希望通过鲤鱼品种间杂交出现的杂种优势来提高鲤鱼的产量以及在杂种的后代中选择具有优良的经济性状的类型培育出新的鲤鱼品种。两组杂交的杂种一代具有明显的杂种优势,可以直接应用于渔业生产上并可望获得增产效果。根据对鳞被和体色的遗传特性的研究结果断定散鳞镜鲤与兴国红鲤的杂种二代中出现的红色镜鲤的鳞被和体色是由纯合型隐性基因控制的,可以预期它的后代不再出现分离而可能成为一个稳定的新品种。研究了不同品种及其杂种的血清蛋白聚丙烯酰胺盘状电泳图谱,希望借此作为一种选种的工具。 | Subgroup of Carp Studies,Section of Fish Breeding,Laboratory of Fish Genetics Breeding,Institute of Hydrobiology,Hupei Province | 1975 | 水生生物学集刊1975，5，4： | 11 |
| 8 | Catalytic performance of Fe_3O_4-CoO/Al_2O_3 catalyst in ozonation of 2-(2,4-dichlorophenoxy) propionic acid,nitrobenzene and oxalic acid in water显示文摘Fe3O4-CoO/Al2O3 catalyst was prepared by incipient wetness impregnation using Fe(NO3) 3·9H2O and Co(NO3) 2·6H2O as the precursors,and its catalytic performance was investigated in ozonation of 2-(2,4-dichlorophenoxy) propionic acid(2,4-DP) ,nitrobenzene and oxalic acid.The experimental results indicated that Fe3O4-CoO/Al2O3 catalyst enabled an interesting improvement of ozonation eciency during the degradation of each organic pollutant,and the Fe3O4-CoO/Al2O3 catalytic ozonation system followed a radical-type mechanism.The kinetics of ozonation alone and Fe3O4-CoO/Al2O3 catalytic ozonation of three organic pollutants in aqueous solution were discussed under the mere consideration of direct ozone reaction and OH radical reaction to well investigate its performance.In the catalytic ozonation of 2,4-DP,the apparent reaction rate constants(k) were determined to be 1.456×10-2 min-1 for ozonation alone and 4.740×10-2 min-1 for O3/Fe3O4-CoO/Al2O3.And O3/Fe3O4-CoO/Al2O3 had a larger Rct(6.614×10-9) calculated by the relative method than O3 did(1.800×10-9) ,showing O3/Fe3O4-CoO/Al2O3 generated more hydroxyl radical.Similar results were also obtained in the catalytic ozonation of nitrobenzene and oxalic acid.The above results demonstrated that the catalytic performance of Fe3O4-CoO/Al2O3 in ozonation of studied organic substance was universal to a certain degree. | Shaoping Tong,Rui Shi,Hua Zhang,Chunan Ma College of Chemical Engineering and Materials Science,State Key Laboratory Breeding Base of Green Chemistry-Synthesis Technology,Zhejiang University of Technology,Hangzhou 310032,China | 2010 | Journal of Environmental Sciences2010，22，10： | 10 |
| 9 | 细鳞斜颌鲴的养殖及其生物学研究显示文摘细鳞斜颌鲴是在我国分布较广而又为群众所喜爱的一种野生经济鱼类。这种鱼在某些天然水体中可成为优势种群,产量较大,最高可以占总渔获量的50%以上。但有许多水体中却没有这种鱼或数量不大。通过试验和研究,我们已能对这种鱼进行大规模的人工繁殖和苗种培育,近年来,已将数万尾大规格鱼种投放到一原来只投放“家鱼”(泛指鲩、青、鲢、鳙,以下均同)的水库中,并将检验其增产效果。实践证明斜颌鲴在池塘中与现有“家鱼”混养时,非但不影响“家鱼”的生长,并可使池塘总产量增加10%以上;原因是这种鱼主要以其他养殖鱼类尚未利用或未能充分利用的腐屑、腐泥和着生藻类为食物。该鱼头两年生长较快,两周年可达一市斤左右,并第一次达到性成熟,这种规格的鱼上市就是经济价值高的优良食用鱼。因其在池塘中易于捕捞,故也是丰产塘中轮捕轮放的好对象。按普通“家鱼”种的培育方法当年鱼可养成4寸左右,第二年底都能在半斤以上,大的可达8两半。由于该鱼头小肉多而味美,故即使2—3两重的鱼在市场上也受到顾客的欢迎。本文还介绍了养殖这种鱼的方法及其有关的生物学资料,并讨论了在天然水体中促使这种鱼增产的可能途径。 | Administrative Board of the Tao-Yuan-Ho Reservoir,Hupei Province Section of Introduction Acclimation,Laboratory of Fish Genetics Breeding,Institute of Hydrobiology,Hupei Province | 1975 | 水生生物学集刊1975，5，4： | 10 |
| 10 | Morphology and mapping analysis of rice(Oryza sativa L.)clustered spikelets(Cl)mutant显示文摘The rice clustered spikelets (Cl) mutant exhib-its a phenotype that most of branch apical have 2-3 spikelets clustered together. SEM (scanning electron microscope) ob-servation suggested that the Cl gene controlled branch apical development, and influenced the terminal spikelets elonga-tion. The spikelet number was reduced in mutant, indicating that Cl may also have an effect on spikelet number. To map Cl locus, two F2 mapping populations derived from the crosses between the Cl and ZhongHua11, and Cl and ZheFu802 were constructed, respectively. The Cl locus was roughly mapped between two CAPS markers, CK0214 and SS0324. A further fine mapping analysis showed that the Cl locus was mapped between makers R0674E and Cl2560, with genetic distances of 0.2 and 2.1 cM, respectively. Then we found a PAC contig spanning Cl locus, the region was delim-ited to 196 kb. This result was useful for cloning of the Cl gene. Allelism test demonstrated that Cl was allelic to Cl2, another rice clustered spikelets mutant. | ZHENG Leiying1, ZHU Xudong2, QIAN Qian2, ZHAO Zhong1, ZHANG Jianjun3, HU Xiaohe1, LIN Hongxuan1,4 & LUO Da1 1. National Key Laboratory of Plant Molecular Genetics, Shanghai In-stitute of Plant Physiology and Ecology, Chinese Academy of Sci-ences, Shanghai 200032, China 2. China National Rice Research Institute, Hangzhou 310006, China 3. Crop Institute of Breeding and Cultivation, Shanghai Agricultural Academy, Shanghai 201106, China 4. Shanghai Anxin Research Foundation Laboratory, Shanghai 200032, China | 2003 | Chinese Science Bulletin2003，48，6： | 10 |
| 11 | Expression of the Tribolium castaneum (Coleoptera: Tenebrionidae) hsp83 gene and its relation to oogenesis during ovarian maturation显示文摘Heat shock proteins (HSP) can protect organisms and cells from thermal damage. In this study, we cloned the full length cDNA encoding the HSP83 protein (the homologue of HSP90) of Tribolium castaneum (red flour beetle). The isolated cDNA contains the full coding sequence, a partial 5′ untranslated region of 55 bp and the complete 3′ untranslated region. We found the hsp83 gene is located on chromosome 5 of the T. castaneum genome. The predicted HSP83 protein sequence has a high similarity (on average 86.77%) with that of other insect species. The expression of the hsp83 gene in the whole body and in the ovary could be induced with heat stress (40°C for 1 h) in newly hatched (within 3 h post emergence) and mature (10 days post emergence) beetles. Under normal conditions, the hsp83 expression in the ovary is about 3-fold higher than in the whole body at both stages. No significant difference in hsp83 expression was observed between the two ovarian developmental stages regardless if the beetles were treated with heat shock or not. The expression of the HSP83 protein in the whole body could also be induced with heat stress in newly hatched and mature beetles. However, in the ovary, HSP83 was only expressed in the follicle cells of mature beetles and not in newly hatched beetles, regardless if the beetles were treated with heat shock or not. Furthermore, the females were not able to produce mature oocytes after knock-down of the hsp83 expression by injecting dsRNA. These results suggest that the HSP83 protein is involved in protection against heat stress and could be involved in oogenesis during ovarian maturation of T. castaneum. | Jingjing Xu 1, Juan Shu 1, Qin Zhang Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China | 2010 | Journal of Genetics and Genomics2010，37，8： | 9 |
| 12 | Characterization of interspecific hybrids and backcross progenies from a cross between Oryza minuta and Oryza sativa显示文摘Oryza minuta, a tetraploid wild relative of cultivated rice, is an important source for the genetic improvement. Interspecific hybrids were obtained from the cross of O. sativa L. (IR24) and O. minuta (Acc. No. 101133) with 5.58% crossability, which ranged from 0.11% to 1.62% in the backcross generations. The chromosome numbers of the backcross progenies were 24 to 48. Seven yield-related traits of the parents, hybrid F1, and backcross progenies were evaluated. Simple sequence repeat markers analysis showed that the polymorphism ratio of SSR bands between IR24 and Acc. No. 101133 was 93.2%. The average donor segment number, length, donor genome size, and percentage of donor genome of 92 BC3F1 plants (2n=24) were 24.1, 17.8 cM, 438.4 cM and 26.2%, respectively. They were complex variation and uneven among the chromosomes. These introgression lines could be used to identify the favorable genes of O. minuta and provide a new platform for the genetic improvement of cultivated rice. | GUO SiBin, QIN FaLan, ZHANG DuanPin & LIN XingHua· National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan 430070, China | 2009 | Science China(Life Sciences)2009，52，12： | 9 |
| 13 | 图书馆系统市场报告(2021):在充满挑战的时代中推进图书馆技术显示文摘美国图书馆专家Marshall Breeding每年根据图书馆技术相关公司的调查报告,结合市场公开信息,总结发布图书馆系统市场年度报告。本文为2021年度报告的译文,介绍了在新冠疫情影响下,图书馆系统行业的市场动态、业绩表现、公司发展、开源产品,以及各类型图书馆系统的市场分析,详细说明各公司的产品动态和发展趋势。报告有助于我国图书馆了解当前图书馆技术行业的环境变化和发展趋势,了解国外厂商的主要产品,对我国图书馆系统厂商的发展也有借鉴意义。 | Marshall Breeding 肖铮(译) 施晓华(译) 许敬一(译) | 2021 | 图书馆论坛2021，41，10： | 8 |
| 14 | Genetical and Physiological Basis of Plant Type Model of Erect and Large Panicle Japonica Super Rice in Northern China显示文摘The historical changes in rice yields across China were explored. The physiological mechanisms and genetic basis of the erect and large panicle super-high-yield plant type model for breeding japonica super rice were analyzed mainly on the panicle type, number of large vascular bundles (LVB) in the panicle neck, and the panicle type index (PTI). In the production point of view, we suggested that, for the breeding of super-high-yield japonica rice, the erect panicle types with more LVB numbers in the panicle neck and superior upper grains in the secondary branches would be the key factors. The information has potential significance in the rice breeding and productivity not only in China but also throughout the rice production areas of the world. | XU Zheng-jin, CHEN Wen-fu, HUANG Rui-dong, ZHANG Wen-zhong, MA Dian-rong, WANG Jia-yu, XU Hai and ZHAO Ming-hui Key Lab of Northern Japonica Rice Breeding of Liaoning Province, Shenyang Agricultural University, Shenyang 110161, P.R.China | 2010 | Agricultural Sciences in China2010，9，4： | 7 |
| 15 | Plant Variety Protection and DUS Testing Technique显示文摘DUS testing technique used for plant variety protection was reviewed in the paper, and somesuggestions were made on how to establish the appropriate technology system in China. Meanwhile, the poten-tial exploitation of the technique was discussed. | LI Xiao-hui, LI Xin-hai, ZHANG Shi-huang, LI Ming-shun and LI Wen-hua(Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences /Key Laboratory of CropGenetics and Breeding , Ministry of Agriculture/AMBIONET-China Laboratory , Beijing 100081 , P. R. China College of Agronomy , Northeast Agricultural University, Harbin 150030, P.R.China) | 2002 | Agricultural Sciences in China2002，1，12： | 6 |
| 16 | 创新的周期:2019年图书馆系统市场报告显示文摘一般说来,图书馆技术行业更偏向实用性而非创新性。图书馆自动化系统并不是一个会让人兴奋的技术,但它们却是图书馆最主要的应用程序,必须要支持图书馆资源与服务的描述、获取、访问等一系列复杂任务。它们是图书馆的一笔大投资,图书馆每天都在检验它的使用效果。 | Marshall Breeding 肖铮 | 2019 | 图书馆论坛2019，39，8： | 6 |
| 17 | 2020图书馆系统市场报告:整合中的新机遇显示文摘美国图书馆专家Marshall Breeding根据其2019年度图书馆技术公司调查报告和市场上公开的信息,撰写了图书馆行业技术产品市场报告。报告从行业市场动态、业绩表现、公司发展、开源产品和各类型图书馆市场分析等方面阐述各公司的产品动态和发展趋势。报告对我国图书馆界了解图书馆技术行业发展趋势、国外各厂商主要产品信息等具有参考价值。 | Marshall Breeding 肖铮(译) | 2020 | 图书馆论坛2020，40，10： | 6 |
| 18 | Cloning and characterization of a novel R1-MYB transcription factor in maize显示文摘R1-MYB transcription regulatory factors play vital roles in transcriptional modification during higher plant metabolism and development. In the present study, an R1-MYB gene was isolated from the maize (Zea mays L.) inbred line C8605-2 based on expressed sequence tags (ESTs), which expressed differentially between the hybrid C8605-2 × W1445 and its two parents in an oligonucleotide microarray. The full-length cDNA, designated as ZmMYBE1 (GeneBank Accession No. FJ024049), consists of 1992 nucleotides and contains a 1296-bp open reading frame. One conserved MYB domain was detected near the N-terminus of the deduced amino acid sequence, where an acidic Ser/Thr-rich area was also observed in the downstream region. The sequence of ZmMYBE1, mapped to chromosome 5 (bin 5.03), was revealed belonging to a multi-gene family in the maize genome. Subcellular localization analysis determined that ZmMYBE1 was located in the nucleus. Expression analysis showed that ZmMYBE1 transcripts accumulated in various tissues examined, with high expression levels in the immature ear and low levels in the tassel, root, and stem. The expression analysis for ZmMYBE1 in immature ears showed the highest expression level at the earliest vegetative developmental stage of the plant. Expression of ZmMYBE1 higher than that of the parents was observed in the hybrid C8605-2 × W1445. And also the expression level similar to the lowest of the parents in the hybrid C8605-2 × W245 was observed, revealing different expression patterns for the same gene in various hybrids combined from diversified inbred lines. Transgenic Arabidopsis overexpressing ZmMYBE1 implies that ZmMYBE1 has an important role in developmental regulation in maize. | Guanqing Jia, Bo Li, Dengfeng Zhang, Tifu Zhang, Zhiyong Li, Jingrui Dai, Shoucai Wang* National Maize Improvement Center of China, Key Laboratory of Crop Genomics and Genetic Improvement of Agriculture Ministry, Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China | 2009 | Progress in Natural Science:Materials International2009，19，9： | 6 |
| 19 | Effect of HMW-GS 5+10 on Quality Parameters in Four Leading Wheat Cultivars显示文摘HMW-GS 5+ 10 was introduced to four major wheat cultivars Kehan9, Kefeng3, Longmai20and Kenda4 through 5 or 6 consecutive backcrosses. No significant difference in protein content and dry glutencontent was observed between cultivars containing 5+ 10 and 2+ 12 or 3+12 (P>0. 1). In cuitivars contai-ning HMW-GS 5 +10, the ratio of wet gluten content to dry gluten content was 2.9 -5.0 % (P<0.01) lowerthan the ratio from the cultivars containing 2+ 12 or 3+12, the ratio of Zeleny sedimentation volume to drygluten content was 4.5 - 13.4% (P<0. 05) higher. The degree of softening in cuitivars having 5+10 was 15- 25 FU (P<0.01) lower, while the maximum resistance was 82 - 193 EU (P<0.05) higher. Backcrosseswith biochemical marker assisted selection in the improvement of quality in wheat cultivars was discussed. | ZHANG Yan-bin, SUN Lian-fa, XIN Wen-li, SONG Qin-jie, ZHANG Chun-li, ZHAO Hai-bin, XIAO Zhi-min and QI Shi-yuCrop Breeding Institute , Heilongjiang Academy of Agricultural Sciences , Harbin 150086 , P. R. China | 2003 | Agricultural Sciences in China2003，2，5： | 6 |
| 20 | Effects of Lipoxygenase Null Genes of Soybean in Controlling Beany-flavor of Soymilk and Soyflour显示文摘The flavor of the soymilk and soyflour obtained from the lipoxygenase mutant isolines was concentrated by simultaneous distillation and extraction (SDE), and its constituents were identified by gas chromatography (GC) and gas chromatography-mass spectrometry. Results showed that the same 24 flavor constituents were isolated in both soymilk and soyflour, and most of them were aldehydes and alcohols. Lox2 was most responsible for the production of the volatile and beany-flavor components, and Lox1 less responsible.Lox3 was least responsible and can reduce the yield of hexanal. Either Lx1 or Lx2 could significantly reduce the volatile and beany-flavor, and Lx3 could significantly increase the yield of hexanal. Primary and secondary interactions existed among the null mutant genes, and the major effects and interactions could be affected by processing conditions. The isoline with triple lipoxygenase null genes yielded the least volatile and beany-flavor components, and the isoline without the lipoxygenase gene Lx3 produced the greatest amount of the volatile and beany-flavor components. The amounts of volatile and beany-flavor components produced by the other isolines were between that of the isoline with triple lipoxygenase null genes and the isoline without lipoxygenase gene Lx3. According to the correlation analysis, the hexanal amount could be used as an index in evaluating the importance of lipoxygenase isozymes in the yield of beany-flavor compounds, and the effects of the different types of lipoxygenase null mutants in controlling beany-flavor compounds. The cultivars with triple lipoxygenase null genes will be a quality raw material for soyfood processing. | MA Hao, GUAN Chun-yun,HE Xiao-ling, ZHANG Guo-zheng and DIN An-lin(College of Plant Sciences and Technology , Hunan Agricultural University, Changsha 410128 ,P. R. China Soybean ResearchInstitute , Nanjing Agricultural University, Nanjing 210095 ,P.R. China Institute of Crop Breeding and Cultivation ,Chinese Academy of Agricultural Sciences , Beijing 100081 ,P.R. China ) | 2002 | Agricultural Sciences in China2002，1，9： | 5 |
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