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| 1 | Degradation of lipid droplets by chimeric autophagy-tethering compounds显示文摘Degrading pathogenic proteins by degrader technologies such as PROTACs(proteolysis-targeting chimeras)provides promising therapeutic strategies,but selective degradation of non-protein pathogenic biomolecules has been challenging. | Yuhua Fu Ningxie Chen Ziying Wang Shouqing Luo Yu Ding Boxun Lu | 2021 | Cell Research2021，31，9： | 5 |
| 2 | Suppression of MAPK11 or HIPK3 reduces mutant Huntingtin levels in Huntington's disease models显示文摘 | Meng Yu Yuhua Fu Yijian Liang Haikun Song Yao Yao Peng Wu Yuwei Yao Yuyin Pan Xue Wen Lixiang Ma Saiyin Hexige Yu Ding Shouqing Luo Boxun Lu | 2017 | Cell Research2017，27，12： | 5 |
| 3 | Tau Accumulation via Reduced Autophagy Mediates GGGGCC Repeat Expansion-Induced Neurodegeneration in Drosophila Model of ALS显示文摘Expansions of trinucleotide or hexanucleotide repeats lead to several neurodegenerative disorders,including Huntington disease[caused by expanded CAG repeats(CAGr)in the HTT gene],and amyotrophic lateral sclerosis[ALS,possibly caused by expanded GGGGCC repeats(G4C2r)in the C9ORF72 gene],of which the molecular mechanisms remain unclear.Here,we demonstrated that lowering the Drosophila homologue of tau protein(dtau)significantly rescued in vivo neurodegeneration,motor performance impairments,and the shortened life-span in Drosophila expressing expanded CAGr or expanded G4C2r.Expression of human tau(htau4 R)restored the disease-related phenotypes that had been mitigated by the loss of dtau,suggesting an evolutionarily-conserved role of tau in neurodegeneration.We further revealed that G4C2r expression increased tau accumulation by inhibiting autophagosome-lysosome fusion,possibly due to lowering the level of BAG3,a regulator of autophagy and tau.Taken together,our results reveal a novel mechanism by which expanded G4C2r causes neurodegeneration via an evolutionarily-conserved mechanism.Our findings provide novel autophagy-related mechanistic insights into C9ORF72-ALS and possible entry points to disease treatment. | Xue Wen Ping An Hexuan Li Zijian Zhou Yimin Sun Jian Wang Lixiang Ma Boxun Lu | 2020 | Neuroscience Bulletin2020，36，12： | 4 |
| 4 | M3SC:A Generic Dataset for Mixed Multi-Modal(MMM)Sensing and Communication Integration显示文摘The sixth generation(6G)of mobile communication system is witnessing a new paradigm shift,i.e.,integrated sensing-communication system.A comprehensive dataset is a prerequisite for 6G integrated sensing-communication research.This paper develops a novel simulation dataset,named M3SC,for mixed multi-modal(MMM)sensing-communication integration,and the generation framework of the M3SC dataset is further given.To obtain multimodal sensory data in physical space and communication data in electromagnetic space,we utilize Air-Sim and WaveFarer to collect multi-modal sensory data and exploit Wireless InSite to collect communication data.Furthermore,the in-depth integration and precise alignment of AirSim,WaveFarer,andWireless InSite are achieved.The M3SC dataset covers various weather conditions,multiplex frequency bands,and different times of the day.Currently,the M3SC dataset contains 1500 snapshots,including 80 RGB images,160 depth maps,80 LiDAR point clouds,256 sets of mmWave waveforms with 8 radar point clouds,and 72 channel impulse response(CIR)matrices per snapshot,thus totaling 120,000 RGB images,240,000 depth maps,120,000 LiDAR point clouds,384,000 sets of mmWave waveforms with 12,000 radar point clouds,and 108,000 CIR matrices.The data processing result presents the multi-modal sensory information and communication channel statistical properties.Finally,the MMM sensing-communication application,which can be supported by the M3SC dataset,is discussed. | Xiang Cheng Ziwei Huang Lu Bai Haotian Zhang Mingran Sun Boxun Liu Sijiang Li Jianan Zhang Minson Lee | 2023 | China Communications2023，20，11： | 3 |
| 5 | AlphaLISA detection of alpha-synuclein in the cerebrospinal fluid and its potential application in Parkinson's disease diagnosis显示文摘 | Zhao, Hongli Zhao, Jue Hou, Jiapeng Wang, Siqing Ding, Yu Lu, Boxun Wang, Jian | 2017 | Protein & Cell2017，8，9： | 2 |
| 6 | Degeneration Versus Development: Hunting-Out the D-Unit of Huntington's Disease显示文摘Huntington’s disease (HD) is an autosomal dominant neurodegenerative disorder caused by a CAG trinucleotide repeat expansion exceeding a threshold length (>35repeats) in exon 1 of the huntingtin gene (HTT)[1]. The age at onset is typically 40–50 years, except for a very low percentage (approximately 6%) of juvenile-onset HD patients carrying 75 or more CAG repeats;longer CAG repeats predict an earlier onset [1]. | Shengyi Lu Boxun Lu | 2021 | Neuroscience Bulletin2021，37，5： | 1 |
| 7 | A high-throughput-compatible assay to measure the degradation of endogenous Huntingtin proteins显示文摘瞄准:引起疾病的蛋白质的累积是许多 neurodegenerative 混乱的一个普通特点。用 high-throughput-compatible 试金测量如此的蛋白质的降级高度为降级的基因、化学的调节的人的鉴定被需要。例如, Huntingtons 疾病(HD ) 是变异的 huntingtin 蛋白质(mHTT ) 的 cytotoxicity 引起的医不好的世袭 neurodegenerative 混乱。mHTT 降级的高产量的测量在 HD 药发现和研究是重要的。为如此的目的存在方法在蛋白质标签或平底锅蛋白质合成禁止者上由于他们的依赖有限制。这里,我们为蛋白质(HTT ) 降级基于按化学和同类的时间解决了的内长的没有标签的 huntingtin 的测量报导一个 high-throughput-compatible 脉搏追逐方法(CH 追逐) 荧光(HTRF ) technologies.Methods:标记脉搏的蛋白质用按反应与 biotin 被结合支持紧张的炔属羟叠氮化物 cycloaddition (SPAAC ) ,和追逐信号被与 streptavidin beads.Results 在下拉以后测量 HTT HTRF 信号的减小百分比计算:我们验证了信号在线性察觉范围以内并且是 HTT 特定的。我们成功地用 96 井盘子在一种 high-throughput-compatible 格式测量了内长的 HTT 的降级。由已知的修饰词的 HTT 降级的预言的变化被观察,它证实试金对 HTT 降级 modifiers.Conclusion 的鉴定合适:我们建立了能够测量内长的、没有标签的 HTT 降级的第一 high-throughput-compatible 试金,提供为 HD 研究和药发现的一个珍贵工具。方法能被用于另外的蛋白质并且能在另外的 neurodegenerative 混乱和 proteinopathies 上便于研究。 | Peng WU Ming-xing LU Xiao-tian CUI He-qing YANG Shen-liang YU Jian-bin ZHU Xiao-li SUN Boxun LU | 2016 | Acta Pharmacologica Sinica2016，37，10： | 1 |
| 8 | Effects of Mn O on Crystallization,Melting,and Heat Transfer of Ca O-Al2O3-Based Mold Flux Used for High Al-Trip Steel Casting显示文摘 | Huan Zhao Wanlin Wang Lejun Zhou Boxun Lu Youn-Bae Kang | 2014 | Metallurgical and Materials Transaction B2014，45，4： | 1 |
| 9 | Modeling the Degradation Effects of Autophagosome Tethering Compounds显示文摘Dear Editor,The selective degradation of specific pathogenic proteins provides exciting strategies for drug discovery.Emerging new concepts such as proteolysis-targeting chimeras and autophagosome-tethering compounds(ATTECs)are based on the design of'molecular glues'or bifunctional chimeric compounds that tether the target protein(protein of interest,POI)to a specific component of the proteindegradation machinery(PDM)[1-3]. | Hang Zhang Ping An Yiyan Fei Boxun Lu | 2021 | Neuroscience Bulletin2021，37，2： | 1 |
| 10 | Autophagic Clearance of Lipid Droplets Alters Metabolic Phenotypes in a Genetic Obesity–Diabetes Mouse Model显示文摘Lipid droplets(LDs)are intracellular organelles that store neutral lipids,and their aberrant accumulation is associated with many diseases including metabolic disorders such as obesity and diabetes.Meanwhile,the potential pathological contribu-tions of LDs in these diseases are unclear,likely due to a lack of chemical biology tools to clear LDs.We recently developed LD-clearance small molecule compounds,Lipid Droplets·AuTophagy TEthering Compounds(LD·ATTECs),that are able to induce autophagic clearance of LDs in cells and in the liver of db/db(C57BL/6J Leprdb/Leprdb)mouse model,which is a widely used genetic model for obesity–diabetes.Meanwhile,the potential effects on the metabolic phenotype remain to be elucidated.Here,using the metabolic cage assay and the blood glucose assay,we performed phenotypic characteriza-tion of the effects of the autophagic degradation of LDs by LD·ATTECs in the db/db mouse model.The study reveals that LD·ATTECs increased the oxygen uptake of mice and the release of carbon dioxide,enhanced the heat production of animals,partially enhanced the exercise during the dark phase,decreased the blood glucose level and improved insulin sensitivity.Collectively,the study characterized the metabolic phenotypes induced by LD·ATTECs in an obesity–diabetes mouse model,revealing novel functional impacts of autophagic clearance of LDs and providing insights into LD biology and obesity–dia-betes pathogenesis from the phenotypic perspective. | Ningxie Chen Boxun Lu Yuhua Fu | 2023 | Phenomics2023，3，2： | 0 |
| 11 | Hunting out the repeat expansion in Huntington's pigs显示文摘A recent study from Yan et al.(2023)provides the milestone evidence in the large animal model demonstrating genome editing as a potential therapeutic strategy for Huntington's disease treatment. | Guang Yang Boxun Lu | 2023 | Protein & Cell2023，14，12： | 0 |
| 12 | Inhibition of HIPK3 by AST487 Ameliorates Mutant HTT-Induced Neurotoxicity and Apoptosis via Enhanced Autophagy显示文摘Dear Editor,Accumulation of misfolded and aggregation-prone proteins is the common hallmark of many neurodegenerative disorders,and lowering the levels of these proteins may provide promising strategies for the potential treatment of some of these diseases[1,2].Among them,Huntington’s disease(HD)is a monogenic disease caused by mutation of the HTT(huntingtin)gene[3],which encodes the mutant HTT protein(mHTT)with an expanded polyglutamine tract(polyQ). | Xiaodan Zhang Xue Wen Ismael Al-Ramahi Juan Botas Boxun Lu Yuhua Fu | 2022 | Neuroscience Bulletin2022，38，1： | 0 |
| 13 | Correction:Inhibition of HIPK3 by AST487 Ameliorates Mutant HTT-Induced Neurotoxicity and Apoptosis via Enhanced Autophagy显示文摘We are sorry that we have made a few minor errors regarding the description of the scale bars and data presentation in the figure legends(not the figures)of the following manuscript:We’d like to make the following corrections:The authors would like to correct the following errors in the figure legends of Fig.1B–1D and Fig.2F–2I by changing“mean±SEM”to“mean±SD”. | Xiaodan Zhang Xue Wen Ismael Al-Ramahi Juan Botas Boxun Lu Yuhua Fu | 2022 | Neuroscience Bulletin2022，38，9： | 0 |
| 14 | Optogenetic control of GGGGCC repeat-containing RNA phase transition显示文摘The GGGGCC(G_(4)C_(2))hexanucleotide repeat expansion in the C9ORF72 gene is a major cause of both hereditary amyotrophic lateral sclerosis and familial frontotemporal dementia.Recent studies have shown that G_(4)C_(2)hexanucleotide repeat-containing RNA transcripts((G_(4)C_(2))_(n)RNA)could go through liquid-liquid phase separation to form RNA foci,which may elicit neurodegeneration.However,the direct causality between these abnormal RNA foci and neuronal toxicity remains to be demonstrated.Here we introduce an optogenetic control system that can induce the assembly and phase separation of(G_(4)C_(2))_(n)RNA foci with blue light illumination in human cells,by fusing a specific(G_(4)C_(2))_(n)RNA binding protein as the linker domain to Cry2,a protein that oligomerizes in response to blue light.Our results demonstrate that a higher number of G_(4)C_(2)repeats have the potential to be induced into more RNA foci in the cells.Both spontaneous and induced RNA foci display liquid-like properties according to FRAP measurements.Computational simulation shows strong consistency with the experimental results and supports the effect of our system to promote the propensity of(G_(4)C_(2))_(n)RNA towards phase separation.This system can thus be used to investigate whether(G_(4)C_(2))_(n)RNA foci would disrupt normal cellular processes and lead to pathological phenotypes relevant to repeat expansion disorders. | Xiong Li Shengyi Lu Boxun Lu Xiaoli Sun | 2022 | Fundamental Research2022，2，6： | 0 |
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