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6篇 您的检索式:作者名="Boris Chichkov"
    题名 作者 年代 出处 被引量
1High-aspect 3D two-photon polymerization structuring with widened objective working range (WOW-2PP)显示文摘We developed a novel two-photon polymerization(2PP)configuration for fabrication of high-aspect three-dimensional(3D)structures,with an overall height larger than working distance of the microscope objective used for laser beam focusing into a photosensitive material.This method is based on a modified optical 2PP setup,where a microscope objective(1003 high N.A.),immersion oil and cover glass can be moved together into the photosensitive material,resulting in an effective higher and wider objective working range(WOW-2PP).The proposed technique enables the fabrication of high-aspect structures with sub-micrometer process resolution.3D structures with a height of 7 mm are demonstrated,which could hardly be built with the conventional 2PP set-up due to refractive index mismatch and laser beam disturbances.Kotaro Obata Ayman El-Tamer Lothar Koch Ulf Hinze Boris N Chichkov 2013Light(Science & Applications)2013,2,1:7
2Laser-assisted bioprinting at different wavelengths and pulse durations with a metal dynamic release layer:A parametric study显示文摘For more than a decade,living cells and biomaterials(typically hydrogels)are printed via laser-assisted bioprinting.Often,a thin metal layer is applied as laser-absorbing material called dynamic release layer(DRL).This layer is vaporized by focused laser pulses generating vapor pressure that propels forward a coated biomaterial.Different lasers with laser wavelengths from 193 to 1064 nanometer have been used.As a metal DRL gold,silver,or titanium layers have been used.The applied laser pulse durations were usually in the nanosecond range from 1 to 30 ns.In addition,some studies with femtosecond lasers have been published.However,there are no studies on the effect of all these lasers parameters on bioprinting with a metal DRL,and on comparing different wavelengths and pulse durations–except one study comparing 500 femtosecond pulses with 15 ns pulses.In this paper,the effects of laser wavelength(355,532,and 1064 nm)and laser pulse duration(in the range of 8 to 200 ns)are investigated.Furthermore,the effects of laser pulse energy,intensity,and focal spot size are studied.The printed droplet volume,hydrogel jet velocity,and cell viability are analyzed.Lothar Koch Ole Brandt Andrea Deiwick Boris Chichkov 2017International Journal of Bioprinting2017,3,1:3
3Optical properties of waveguides fabricated in fused silica by femtosecond laser pulses显示文摘Matthias Will Stefan Nolte Boris N Chichkov 2002Applied Optics2002,41,21:1
4Colony development of laser printed eukaryotic(yeast and microalga)microorganisms in co-culture显示文摘Laser Induced Forward Transfer(LIFT)bioprinting is one of a group of techniques that have been largely applied for printing mammalian cells so far.Bioprinting allows precise placement of viable cells in a defined matrix with the aim of directed three-dimensional development of tissues.In this study,laser bioprinting is used to precisely place eukaryotic microorganisms in specific patterns that allow growth and microscopic observation of the organisms’micro-colonies.Saccharomyces cerevisiae var.bayanus and Chlorella vulgaris are used as model organisms for this purpose.Growth and development of the micro-colonies are studied via confocal microscopy and the colonies’growth rates are determined by image analysis.The developed protocols for printing of microorganisms and growth-rate determination of the micro-colonies are very promising for future studies of colony growth and development.Behnam Taidi Guillaume Lebernede Lothar Koch Patrick Perre Boris Chichkov 2016International Journal of Bioprinting2016,2,2:1
5Urokinase Receptor Associates With Myocardin to Control Vascular Smooth Muscle Cells Phenotype in Vascular Disease显示文摘Yulia Kiyan Anne Limbourg Roman Kiyan Sergey Tkachuk Florian P. Limbourg Aleksandr Ovsianikov Boris N. Chichkov Hermann Haller Inna Dumler 2012Arteriosclerosis Thrombosis and Vascular Biology2012,,1:1
6Two-photon polymerization:A new approach to micromachining显示文摘Andreas Ostendorf Boris N Chichkov 0,,:1
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