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| 1 | Near-infrared fluorescence sentinel lymph node detection in gastric cancer: A pilot study显示文摘AIM: To investigate feasibility and accuracy of nearinfrared fluorescence imaging using indocyanine green: nanocolloid for sentinel lymph node(SLN) detection in gastric cancer.METHODS: A prospective, single-institution, phaseI feasibility trial was conducted. Patients suffering from gastric cancer and planned for gastrectomy were included. During surgery, a subserosal injection of 1.6 m L ICG:Nanocoll was administered around the tumor. NIR fluorescence imaging of the abdominal cavity was performed using the Mini-FLARE? NIR fluorescence imaging system. Lymphatic pathways and SLNs were visualized. Of every detected SLN, the corresponding lymph node station, signal-to-background ratio and histopathological diagnosis was determined. Patients underwent standard-of-care gastrectomy. Detected SLNs outside the standard dissection planes were also resected and evaluated.RESULTS: Twenty-six patients were enrolled. Four patients were excluded because distant metastases were found during surgery or due to technical failure of the injection. In 21 of the remaining 22 patients, at least 1 SLN was detected by NIR Fluorescence imaging(mean 3.1 SLNs; range 1-6). In 8 of the 21 patients, tumor-positive LNs were found. Overall accuracy of the technique was 90%(70%-99%; 95%CI), which decreased by higher p T-stage(100%, 100%, 100%, 90%, 0% for respectively Tx, T1, T2, T3, T4 tumors). All NIR-negative SLNs were completely effaced by tumor. Mean fluorescence signal-to-background ratio of SLNs was 4.4(range 1.4-19.8). In 8 of the 21 patients, SLNs outside the standard resection plane were identified, that contained malignant cells in 2 patients.CONCLUSION: This study shows successful use of ICG:Nanocoll as lymphatic tracer for SLN detection in gastric cancer. Moreover, tumor-containing LNs outside the standard dissection planes were identified. | Quirijn RJG Tummers Leonora SF Boogerd Wobbe O de Steur Floris PR Verbeek Martin C Boonstra Henricus JM Handgraaf John V Frangioni Cornelis JH van de Velde Henk H Hartgrink Alexander L Vahrmeijer | 2016 | World Journal of Gastroenterology2016，22，13： | 9 |
| 2 | ATG16L1 and NOD2 polymorphisms enhance phagocytosis in monocytes of Crohn's disease patients显示文摘AIM:To investigate if the presence of relevant genetic polymorphisms has effect on the effectual clearance of bacteria by monocytes and granulocytes in patients with Crohn’s disease(CD).METHODS:In this study,we assessed the differential responses in phagocytosis by measuring the phagocytic activity and the percentage of active phagocytic monocytes and granulocytes in inflammatory bowel disease patients as well as healthy controls.As both autophagy related like 1(ATG16L1)and immunityrelated guanosine triphosphatase gene are autophagy genes associated with CD and more recently nucleo-tide-binding ligomerization domain-containing protein2(NOD2)has been identified as a potent inducer of autophagy we genotyped the patients for these variants and correlated this to the phagocytic reaction.The genotyping was done with restriction fragment length polymorphisms analysis and the phagocytosis was determined with the pHrodo?Escherichia coli Bioparticles Phagocytosis kit for flowcytometry.RESULTS:In this study,we demonstrate that analysis of the monocyte and granulocyte populations of patients with CD and ulcerative colitis showed a comparable phagocytic activity(ratio of mean fluorescence intensity)between the patient groups and the healthy controls.CD patients show a significantly higher phagocytic capacity(ratio mean percentage of phagocytic cells)compared to healthy controls(51.91%±2.85%vs 37.67%±7.06%,P=0.05).The extend of disease was not of influence.However,variants of ATG16L1(WT:2.03±0.19 vs homozygoot variant:4.38±0.37,P<0.009)as well as NOD2(C-ins)(heterozygous variant:42.08±2.94 vs homozygous variant:75.58±4.34(P=0.05)are associated with the phagocytic activity in patients with CD.CONCLUSION:Monocytes of CD patients show enhanced phagocytosis associated with the presence of ATG16L1 and NOD2 variants.This could be part of the pathophysiological mechanism resulting in the disease. | Simone CS Wolfkamp Caroline Verseyden Esther WM Vogels Sander Meisner Kirsten Boonstra Charlotte P Peters Pieter CF Stokkers Anje A te Velde | 2014 | World Journal of Gastroenterology2014，20，10： | 2 |
| 3 | The DNA-binding factor Ctcf critically controls gene expression in macrophages显示文摘巨噬细胞在免疫和动态平衡起一个重要作用。在经由特定的受体的病原体识别之上,他们很快导致煽动性的回答。这个过程紧在 transcriptional 水平被控制。DNA 有约束力的锌手指蛋白质 CCCTC 有约束力的因素(Ctcf ) 是远程的染色质相互作用的一个关键管理者并且协调在抄写因素和基因表达式进程之间的特定的通讯。在这研究, Ctcf 基因被使用转基因的 Cre-LoxP 系统明确地在 myeloid 房间删除。在 myeloid 房间的 Ctcf 基因的有条件的删除在 vivo 导致了温和显型。显著地展出的 Ctcf 缺乏的老鼠减少了主要 histocompatibility 的表示在肝的建筑群(MHC ) 班 II。Ctcf 缺乏的巨噬细胞表明了正常表面显型和吞噬作用能力。在像使用费的受体(TLR ) 之上刺激,他们生产了支持 inflammatory cytokines IL-12 和 IL-6 的正常层次,但是表明了一个强烈损害的能力生产肿瘤坏死因素(TNF ) 和 IL-10,以及表示 IL-10 家庭成员 IL-19, IL-20 和 IL-24。一起拿,我们的数据表明涉及巨噬细胞功能调整的 Ctcf 的一个角色。 | Tatjana Nikolic Dowty Movita Margaretha EH Lambers Claudia Ribeiro de Almeida] Paula Biesta Kim Kreefft Marjolein JW de Bruijn Ingrid Bergen Niels Galjart Andre Boonstra Rudi Hendriks | 2014 | Cellular & Molecular Immunology2014，11，1： | 2 |
| 4 | The response to TLR ligation of human CD16 + CD14 ? monocytes is weakly modulated as a consequence of persistent infection with the hepatitis C virus显示文摘 | Cheng Peng Bi-Sheng Liu Robert J. de Knegt Harry L.A. Janssen André Boonstra | 2011 | Molecular Immunology2011，，12： | 2 |
| 5 | The utility of Ki-67 and BrdU as proliferative markers of adult neurogenesis显示文摘 | Kee N Siva lingam S Boonstra R | 2002 | J Neurosci Methods2002，115，： | 1 |
| 6 | Population declines in the snowshoe hare and the role of stress 显示文摘 | Boonstra R Singleton G R | 1993 | General and Comparative Endocrinology1993，91，： | 1 |
| 7 | Polymorphous lighteruption:A clinical,photobiologic,and follow-up study of l10 patients显示文摘 | Boonstra HE van Weelden H Toonstra J | 2000 | J Am Acad Dermatol2000，42，21： | 1 |
| 8 | 显示文摘 | Barat FJ Cua D J Boonstra A | 2002 | J Exp Med2002，195，： | 1 |
| 9 | UV-oxidation technology for disinfection of recirculation water in protected cultivation显示文摘 | Runia W T Boonstra S | 2004 | Acta Horticulturae2004，644，： | 1 |
| 10 | Characterisation of thermally modfied wood: molecular reasons for wood performance improvement显示文摘 | Tjeerdsma BF Boonstra M Pizzi A | 1998 | Holz als Roh-Und Werkstoff1998，56，3： | 1 |
| 11 | Measuring stress in wildlife:Techniques for quantifying glucocorticoids显示文摘 | Sheriff M J Dantzer B Delehanty B Plame R Boonstra R | 2011 | Oecologia2011，166，： | 1 |
| 12 | Spatial filtering of RFI using a reference antenna 显示文摘 | VEEN Alle-Jan Van Der BOONSTRA Albert-Jan | 2010 | RFI 20102010，3，： | 1 |
| 13 | The influence of the pretrestment of powder on the resulting properties of LaNi5 electrodes显示文摘 | Boonstra A H Bernards T M N | 1990 | J Less Common Metals1990，161，2： | 1 |
| 14 | The impact of predator-induced strees in the snowshoe hare cycles 显示文摘 | Boonstra R Hik D Singleton G R Tinnikov A | 1998 | Ecology Monographs1998，79，5： | 1 |
| 15 | 1alpha,25-Dihydroxyvitamin d3 has a direct effect on naive CD4(+) T cells to enhance the development of Th2 cells显示文摘 | Boonstra A Barrat F J Crain C | 2001 | J Immunol2001，167，9： | 1 |
| 16 | Production of monoclonal antibodies againstswine fever virus and their use in laboratory diagno-sis显示文摘 | WENSVOORT G TERPSTRA C BOONSTRA J | 1986 | Vet Microbiol1986，12，2： | 1 |
| 17 | Cofactor regeneration by a soluble pyridine nucleotide transhydrogenase for biological production of hydromorphone 显示文摘 | Boonstra B Rathbone D A | 2000 | Appl Environ Microbiol2000，66，12： | 1 |
| 18 | 1 alpha,25 -Dihydroxyvitamin D has a direct effect on naive CD ~ T cells to enhance the develop- ment of Th2 cells显示文摘 | Boonstra A Barrat FJ Crain C | 2001 | J lmmunol2001，167，9： | 1 |
| 19 | In-hospitalmortality and three-year survival after repaired acute type Aaortic dissection显示文摘 | Aalberts J J Boonstra P W van den Berg M | 2009 | Neth Heart J2009，17，6： | 1 |
| 20 | Interleukin-17 and CD40-ligand synergistically enhance cytokine and chemokine production by renal epithelial cells显示文摘 | Woltman AM de Haij S Boonstra JG | 2000 | J Am Soc Nephrol2000，11，11： | 1 |
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