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| 1 | Identification and classification of all potential hemolysin encoding genes and their products from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai显示文摘Aim: To identify and classify all potential hemolysin candidates of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai. Methods: All of the potential hemolysin encoding genes were characterized in silico. These genes were cloned and expressed in Escherichia coli. The hemolytic activities of the expressed proteins were assayed observing the hemolysis on sheep blood agar plates. Sphingomyelinase activities of the hemolysin candidates were measured by thin-layer chromatography (TLC) and HPLC for sphingomyelin-hydrolysis.Expression and secretion of the hemolysins in L interrogans were studied by reverse transcription polymerase chain reaction, Western blot, and enzyme-linked immunosorbent assays. Results and Conclusion: The hemolytic activities of hemolysin candidates (LA0327, LA0378, LA1027, LA1029, LA1650, LA3050, LA3937, LA4004) from L interrogans strain Lai were confirmed. They were further divided into two groups, sphingomyelinase hemolysins and non-sphingomyelinase hemolysins, based on their ability to hydrolyze sphingomyelin. Most of these hemolysins were actually expressed in living L interrogans and some of them were secreted into the environment. This study establishes an essential and complete basis for further studying the contribution of hemolysins to the pathogenesis of L interrogans. | Yi-xuanZHAN YanGENG BoBI Jian-yongHE Xiao-kuiGUO Guo-pingZHAO | 2005 | Acta Pharmacologica Sinica2005，26，4： | 12 |
| 2 | Characterization of a novel toxin-antitoxin module,VapBC,encoded by Leptospira interrogans chromosome显示文摘Comparative genomic analysis of the coding sequences (CDSs) of Leptospira interrogans revealed a pair of closely linked genes homologous to the vapBC loci of many other bacteria with respect to both deduced amino acid sequences and operon organizations. Expression of single vapC gene in Escherichia coli resulted in inhibition of bacterial growth,whereas co-expression of vapBC restored the growth effectively. This phenotype is typical for three other characterized toxin-antitoxin systems of bacteria, i.e., mazEF[1], relBE[2] and chpIK[3]. The VapC proteins of bacteria and a thermophilic archeae, Solfolobus tokodaii, form a structurally distinguished group of toxin different from the other known toxins of bacteria. Phylogenetic analysis of both toxins and antitoxins of all categories indicated that although toxins were evolved from divergent sources and may or may not follow their speciation paths (as indicated by their 16s RNA sequences), co-evolution with their antitoxins was obvious. | YiXuanZHANG XiaoKuiGUO ChuanWU BoBI ShuangXiREN ChunFuWU GuoPingZHAO | 2004 | Cell Research2004，14，3： | 6 |
| 3 | Monoclonal immunoscintigraphy for detection of metastasis and recurrence of colorectal cancer显示文摘AIM:To assess the clinical role of monoclonal immunoscintigraphy for the detection of metastasis and recurrence of colorectal cancer.METHODS:Monoclonal immunoscintigraphy was performed in patients operated on for colorectal adenocar-cinoma suspected of local recurrence and metastatic disease.The results were compared with conventional diagnostics.RESULTS:Immunoscintigraphic investigation was done in 53 patients.Tumor recurrence occurred in 38 patients,and was confirmed by other diagnostic modalities in 35.In 15 patients,immunoscintigraphic findings were negative,and confirmed in 14 with other diagnostic methods.Comparative analysis confirmed good correlation of immunoscintigraphic findings and the results of conventional diagnostics and the level of tumor marker carcinoembryonic antigen.Statistical analysis of parameters of radiopharmaceutical groups imacis,indimacis and oncoscint presented homogenous characteristics all of three radiopharmaceuticals.The analysis of immunoscintigraphic target focus was clearly improved using tomography.CONCLUSION:Immunoscintigraphy is highly specific and has a good predictive value in local recurrence of colorectal cancer. | Vera Artiko Ana Koljevic Markovi Dragana obi-aranovi Milorad Petrovi Andrija Anti Mirjana Stojkovi Marinko Zuvela Djordjije aranovi Milica Stojkovi Neboja Radovanovi Danijel Galun Aleksandar Milovanovi Jovica Milovanovi Anica Bobi-Radovanovi Zoran Krivokapic Vladimir Obradovi | 2011 | World Journal of Gastroenterology2011，17，19： | 4 |
| 4 | E24 profile slope stability analysis in Haizhou Opencast Coal Mine of Fuxin显示文摘The E24 profile slope analyzed belongs to a series of excavated slopes of the Haizhou Opencast Coal Mine. It seems to be divided into Downslope Part and Upslope Part. Its profile comprises two noticeable coal seams, called the 8# and 9# weak layers, considered as the potential failure surfaces. In consideration of the actual configuration as in the perspective of any modification, assessing the stability of this slope with various profile forms under given conditions, and assessing the risk of instability and quantifying the influence of earthworks or other modifications to the stability of this slope, have constituted the primordial objectives carried out. From assumed potential failure surfaces, any specific profiles and specified slip surfaces are defined. A factor of safety (FoS) is computed for each specified slip surface; the smallest FoS found corresponds to the least favorable slip surface. The safety factor values obtained are compared to the suggested safety factor. Limit equilibrium methods of vertical slices implemented in Slope/W, computer program for slope stability analyses, have been adopted to perform the E24 slope stability analysis. The safety factor values computed with 9# weak layer are lower than for 8#; the factors of safety obtained with Sarma's method are the smallest; more, without groundwater (long term) overall values are greater than those determined under groundwater condition (short term). The lowest safety factor value is found for a profile depending on an adopted earthwork sequence. The E24 profile slope stability analysis shows the instability risk for the deepest weak layer, and also shows the short and long term stability of this slope for the envisaged earth movements. However it demonstrates the existence of instability risk for any earthwork firstly affecting the downslope part. | Simplice F. BOBY | 2007 | Global Geology2007，10，2： | 3 |
| 5 | Posterior reversibleencephalopathy syndrome (PRES):Features on CT and MRimaging显示文摘 | Hugonnet E Da Ines D Boby H | 2012 | Diagn Interv Imaging2012，94，1： | 1 |
| 6 | Posterior Reversi-ble Encephalopathy Syndrome(PRES):Aspects of CT and MRI imaging显示文摘 | Hugonnet E Da Ines D Boby H | | 0，，1： | 1 |
| 7 | Mesenchymal stem cells: characteris- tics and clinical applications 显示文摘 | Bobis S Jarocha D Majka M | 2006 | Folia Histochem Cytobiol2006，44，4： | 1 |
| 8 | Successful term preg- nancy after B-Lynch compression suture in a previous pregnancy on ac- count of massive primary postpartum hemorrhage显示文摘 | Habek D Vranjes M Bobi C Vukovi CM | 2006 | Fetal Diagn Ther2006，21，5： | 1 |
| 9 | Nasal obstruction and smell impairment in nasal polyp disease: correlation between objective and subjective parameters 显示文摘 | Hox V Bobie S Callebaux I | 2010 | Rhinology2010，48，4： | 1 |
| 10 | Posterior reversible encephalopathy syndrome(PRES):features on CT and MR imaging显示文摘 | Hugonneta E Inesa DD Boby H | 2013 | Diagn Interv Imaging2013，94，1： | 1 |
| 11 | Posterior re- versible encephalopathy syndrome (PRES) : features on CT and MR imaging 显示文摘 | HUGONNET E DA INES D BOBY H | 2013 | Diagnostic and Interventional Imag- ing2013，94，1： | 1 |
| 12 | Mesenchymal stem cells : characteristics and clinical applications 显示文摘 | Bobis S Jarocha D Majka M | 2006 | Folia Histochem Cytobiol2006，44，4： | 1 |
| 13 | An elastic-plastic model for the contact of rough surfaces 显示文摘 | Chang W R Etsion I Boby D B | 1987 | Journal of Tribology1987，109，： | 1 |
| 14 | Blackgram fiber (Phaseolus mungo):mechanism of hypoglycemic action显示文摘 | BOBY R G LEELAMMA S | 2003 | Plant Foods Hum Nutr2003，58，1： | 1 |
| 15 | Mesenchymal stem cells:characteristics and clinical applications显示文摘 | Bobis S Jarocha D Majka M | | 0，，04： | 1 |
| 16 | Mesenchymal stem cells: characteristics and clinical Applications 显示文摘 | Bobis S Jarocha D MajkaM | 2005 | Folia Histochemica Et Cytobiologica2005，44，4： | 1 |
| 17 | Successfulterm pregnancy after B - Lynch compression suture in a previ-ous pregnancy on account of massive primary postpartum hem-orrhage 显示文摘 | Habek D Vranjes M Bobi c Vukovi c M | 2006 | Fetal Diagnosis and Therapy2006，21，5： | 1 |
| 18 | Identification of Defects on Highly Reflective Ring Compo- nents and Analysis Using Machine Vision显示文摘 | BOBY R A SONAKAR P S SINGAPERUMAL M | 2011 | The Internation- al Journal of Advanced Manufacturing Technology2011，52，14： | 1 |
| 19 | N-methyl D-aspartate receptor encephalitis: A new addition to the spectrum of autoimmune encephalitis显示文摘 | Boby Maramattom Anu Jacob | 2011 | Annals of Indian Academy of Neurology2011，，3： | 1 |
| 20 | Detection of intimins alpha, beta, gamma, and delta, four intimin derivatives expressed by attach- ing and effacing microbial pathogens显示文摘 | Adu - Bobie J Frankel G Bain C | 1998 | J Clin Microbiol1998，36，： | 1 |
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