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| 1 | miR-20b, miR-98, miR-125b-1*, and let-7e* as new potential diagnostic biomarkers in ulcerative colitis显示文摘AIM:To use microarray-based miRNA profiling of colonic mucosal biopsies from patients with ulcerative colitis (UC), Crohn's disease (CD), and controls in order to identify new potential miRNA biomarkers in inflammatory bowel disease. METHODS:Colonic mucosal pinch biopsies from the descending part were obtained endoscopically from patients with active UC or CD, quiescent UC or CD, as well as healthy controls. Total RNA was isolated and miRNA expression assessed using the miRNA microarray Geniom Biochip miRNA Homo sapiens (Febit GmbH, Heidelberg, Germany). Data analysis was carried out by principal component analysis and projection to latent structure-discriminant analysis using the SIMCA-P+12 software package (Umetrics, Umea, Sweden). The microarray data were subsequently validated by quantitative real-time polymerase chain reaction (qPCR) performed on colonic tissue samples from active UC patients (n = 20), patients with quiescent UC (n = 19), and healthy controls (n = 20). The qPCR results were analyzed with Mann-WhitneyU test.In silico prediction analysis were performed to identify potential miRNA target genes and the predicted miRNA targets were then compared with all UC associated susceptibility genes reported in the literature. RESULTS:The colonic mucosal miRNA transcriptome differs significantly between UC and controls, UC and CD, as well as between UC patients with mucosal inflammation and those without. However, no clear differences in the transcriptome of patients with CD and controls were found. The miRNAs with the strongest differential power were identified (miR-20b, miR-99a, miR-203, miR-26b, and miR-98) and found to be upregulated more than a 10-fold in active UC as compared to quiescent UC, CD, and controls. Two miRNAs, miR-125b-1* and let-7e*, were up-regulated more than 5-fold in quiescent UC compared to active UC, CD, and controls. Four of the seven miRNAs (miR-20b, miR-98, miR-125b-1*, and let-7e*) were validated by qPCR and found to be specifically upregulated in patients with UC. Usingin silico analysis we found several predicted pro-inflammatory target genes involved in various pathways, such as mitogen-activated protein kinase and cytokine signaling, which are both key signaling pathways in UC.CONCLUSION:The present study provides the first evidence that miR-20b, miR-98, miR-125b-1*, and let7e* are deregulated in patients with UC. The level of these miRNAs may serve as new potential biomarkers for this chronic disease. | Mehmet Coskun Jacob Tveiten Bjerrum Jakob Benedict Seidelin Jesper Thorvald Troelsen JΦrgen Olsen Ole Haagen Nielsen | 2013 | World Journal of Gastroenterology2013，19，27： | 19 |
| 2 | 磷酸掺杂的聚苯并咪唑复合膜在高温质子交换膜燃料电池中的应用(英文)显示文摘研制了聚苯并咪唑(PB I)的复合膜,复合膜中引入的组分包括无机质子导体磷酸氢锆(Zr(HPO4)2.nH2O,ZrP)和聚砜(PSF),以及磺化的酸型聚砜(SPSF(H))。热失重分析表明,复合膜PB I/ZrP/PSF和PB I/SPSF(H)的热稳定性可分别达400和230℃。磷酸掺杂的PB I复合膜在温度高达200℃的低湿度条件下,呈现出高质子电导率。以磷酸掺杂的PB I复合膜为电解质,在200℃常压不加湿的条件下,以氢气氧气为燃料气进行了单电池测试,其最大功率密度分别为0.34 W/cm2(PB I/ZrP/PSF(质量比80∶15∶5))和0.38 W/cm2(PB I/SPSF(0.43H))。 | 何荣桓 李庆峰 Bjerrum Niels J | 2006 | 应用化学2006，23，7： | 2 |
| 3 | Aluminum as anode for energy storage and conversion:a review显示文摘 | LI Q F BJERRUM N J | 2002 | Journal of Power Sources2002，110，： | 1 |
| 4 | Oxygen reduction on carbon supported platinum catalysts in high temperature polymer electrolytes显示文摘 | LI Q F HJULER H A BJERRUM N J | 2000 | Electrochimica Acta2000，45，2526： | 1 |
| 5 | Aluminum as anode for energy storage and conversion: a review显示文摘 | LI Q BJERRUM N J | 2002 | J Power Sources2002，110，： | 1 |
| 6 | Geotechnical problem involved in foundations of structures in the North Sea显示文摘 | Bjerrum J | 1973 | Geotechnique1973，23，3： | 1 |
| 7 | Aluminum as anode for energy storage and conversion:a review显示文摘 | Li Q F Bjerrum N J | 2002 | Journal of Power Sources2002，110，1： | 1 |
| 8 | Massive dissociation of gas hydrate during a Jurassic oceanic anoxic event 显示文摘 | Hesselbo S P Groecke D R Jenkyns H C Bjerrum C J Farrimond P Morgans Bell H S Green O R | 2000 | Nature2000，406，： | 1 |
| 9 | Application of electroosmosis to a foundation problem in Norweygian quick clay 显示文摘 | Bjerrum L Moum J Eide O | 1967 | Geotechnique1967，17，： | 1 |
| 10 | Aluminum as anode for energy storage and conversion: a review显示文摘 | Qingfeng Li Niels J Bjerrum | 2002 | Journal of Power Sources2002，，1： | 1 |
| 11 | Application of electro-Osmosis to a foundation problem in a Norwegian quick clay 显示文摘 | BJERRUM L MOUM J EIDE O | 1967 | Geoteehnique1967，17，3： | 1 |
| 12 | Application of electro- Osmosis to a foundation problem in a Norwegian quick clay显示文摘 | Bjerrum L Moum J Eide O | 1967 | Geotechnigue1967，17，3： | 1 |
| 13 | Phosphoric acid doped polybenzimidazole membranes: Physiochemical characte- rization and fuel cell applications显示文摘 | Li Qingfeng Hjuler H A Bjerrum N J | 2001 | Journal of Applied Electrochemistry2001，31，7： | 1 |
| 14 | Hydraulic fracturing in field permeability testing显示文摘 | BJERRUM L NASH J K T L KENNARD R M | 1972 | Geotechnique1972，22，2： | 1 |
| 15 | Simul- taneous Determination of Glucose, Triglycerides, Urea, Cholesterol, Albumin and Total Protein in Human Plasma by Fourier Transform Infrared Spectroscopy: direct clinical bio- chemistry without reagents 显示文摘 | Jessen T E H? skuldsson A T Bjerrum P J | 2014 | Clinical Biochemistry2014，，47： | 1 |
| 16 | Characterization of recombinant Saccharomyces cerevisiae manganese-containing superoxide dismutase and its H30A and K170R mutants expressed in Escherichia coli显示文摘 | BORDERS C L Jr BJERRUM M J SCHIRMER M A | 1998 | Biochemistry1998，37，11： | 1 |
| 17 | 显示文摘 | Li Q F Hjuler H A Bjerrum N J | 2000 | Electrochim Acta2000，45，： | 1 |
| 18 | Application of electro-osmosis to a foundation problem in a norwegian quick clay显示文摘 | BJERRUM L MOUM J EIDE O | 1967 | Geotechnique1967，17，： | 1 |
| 19 | Phase diagram of the sodium chloride-aluminum chloride system near e- quimolar composition, with determination of the eryo- scopie constant, the enthalpy of melting, mad oxide con-taminations 显示文摘 | Berg R W Hjuler H A Bjerrum N J | 1984 | Inorganic Chemistry1984，23，5： | 1 |
| 20 | Aluminum as anode for energy storage and conversion: a review显示文摘 | LI Q F BJERRUM N J | 2002 | J Power Sources2002，110，： | 1 |
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