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| 1 | miR-20b, miR-98, miR-125b-1*, and let-7e* as new potential diagnostic biomarkers in ulcerative colitis显示文摘AIM:To use microarray-based miRNA profiling of colonic mucosal biopsies from patients with ulcerative colitis (UC), Crohn's disease (CD), and controls in order to identify new potential miRNA biomarkers in inflammatory bowel disease. METHODS:Colonic mucosal pinch biopsies from the descending part were obtained endoscopically from patients with active UC or CD, quiescent UC or CD, as well as healthy controls. Total RNA was isolated and miRNA expression assessed using the miRNA microarray Geniom Biochip miRNA Homo sapiens (Febit GmbH, Heidelberg, Germany). Data analysis was carried out by principal component analysis and projection to latent structure-discriminant analysis using the SIMCA-P+12 software package (Umetrics, Umea, Sweden). The microarray data were subsequently validated by quantitative real-time polymerase chain reaction (qPCR) performed on colonic tissue samples from active UC patients (n = 20), patients with quiescent UC (n = 19), and healthy controls (n = 20). The qPCR results were analyzed with Mann-WhitneyU test.In silico prediction analysis were performed to identify potential miRNA target genes and the predicted miRNA targets were then compared with all UC associated susceptibility genes reported in the literature. RESULTS:The colonic mucosal miRNA transcriptome differs significantly between UC and controls, UC and CD, as well as between UC patients with mucosal inflammation and those without. However, no clear differences in the transcriptome of patients with CD and controls were found. The miRNAs with the strongest differential power were identified (miR-20b, miR-99a, miR-203, miR-26b, and miR-98) and found to be upregulated more than a 10-fold in active UC as compared to quiescent UC, CD, and controls. Two miRNAs, miR-125b-1* and let-7e*, were up-regulated more than 5-fold in quiescent UC compared to active UC, CD, and controls. Four of the seven miRNAs (miR-20b, miR-98, miR-125b-1*, and let-7e*) were validated by qPCR and found to be specifically upregulated in patients with UC. Usingin silico analysis we found several predicted pro-inflammatory target genes involved in various pathways, such as mitogen-activated protein kinase and cytokine signaling, which are both key signaling pathways in UC.CONCLUSION:The present study provides the first evidence that miR-20b, miR-98, miR-125b-1*, and let7e* are deregulated in patients with UC. The level of these miRNAs may serve as new potential biomarkers for this chronic disease. | Mehmet Coskun Jacob Tveiten Bjerrum Jakob Benedict Seidelin Jesper Thorvald Troelsen JΦrgen Olsen Ole Haagen Nielsen | 2013 | World Journal of Gastroenterology2013，19，27： | 19 |
| 2 | MicroRNAs in inflammatory bowel disease-pathogenesis,diagnostics and therapeutics显示文摘The pathogenesis of inflammatory bowel disease (IBD) is complex and largely unknown. Until recently, research has focused on the study of protein regulators in inflammation to reveal the cellular and molecular networks in the pathogenesis of IBD. However, in the last few years, new and promising insights have been generated from studies describing an association between an altered expression of a specific class of non-coding RNAs, called microRNAs (miRs or miRNAs) and IBD. The short (approximately 22 nucleotides), endogenous, single-stranded RNAs are evolutionary conserved inanimals and plants, and regulate specific target mRNAs at the post-transcriptional level. MiRNAs are involved in several biological processes, including development, cell differentiation, proliferation and apoptosis. Furthermore, it is estimated that miRNAs may be responsible for regulating the expression of nearly one-third of the genes in the human genome. Thus, miRNA deregulation often results in an impaired cellular function, and a disturbance of downstream gene regulation and signaling cascades, suggesting their implication in disease etiology. Despite the identification of more than 1900 mature human miRNAs, very little is known about their biological functions and functional targets. Recent studies have identified dysregulated miRNAs in tissue samples of IBD patients and have demonstrated similar differences in circulating miRNAs in the serum of IBD patients. Thus, there is great promise that miRNAs will aid in the early diagnosis of IBD, and in the development of personalized therapies. Here, we provide a short review of the current state-of-the-art of miRNAs in IBD pathogenesis, diagnostics and therapeutics. | Mehmet Coskun Jacob Tveiten Bjerrum Jakob Benedict Seidelin Ole Haagen Nielsen | 2012 | World Journal of Gastroenterology2012，18，34： | 18 |
| 3 | 磷酸掺杂的ab-PBI膜及其在高温质子交换膜燃料电池中的应用显示文摘以3,4-二氨基苯甲酸为单体合成了ab-聚苯并咪唑. 研究了磷酸掺杂的该质子交换膜在80~200 ℃, 不同湿度以及不同酸掺杂量下的质子电导率. 该质子交换膜可作为燃料电池的膜电解质, 在常压不增湿的条件下, 可使电池运行温度达到200 ℃. | 何荣桓 李庆峰 Bjerrum Niels J. | 2005 | 高等学校化学学报2005，26，12： | 11 |
| 4 | 磷酸掺杂的聚苯并咪唑复合膜在高温质子交换膜燃料电池中的应用(英文)显示文摘研制了聚苯并咪唑(PB I)的复合膜,复合膜中引入的组分包括无机质子导体磷酸氢锆(Zr(HPO4)2.nH2O,ZrP)和聚砜(PSF),以及磺化的酸型聚砜(SPSF(H))。热失重分析表明,复合膜PB I/ZrP/PSF和PB I/SPSF(H)的热稳定性可分别达400和230℃。磷酸掺杂的PB I复合膜在温度高达200℃的低湿度条件下,呈现出高质子电导率。以磷酸掺杂的PB I复合膜为电解质,在200℃常压不加湿的条件下,以氢气氧气为燃料气进行了单电池测试,其最大功率密度分别为0.34 W/cm2(PB I/ZrP/PSF(质量比80∶15∶5))和0.38 W/cm2(PB I/SPSF(0.43H))。 | 何荣桓 李庆峰 Bjerrum Niels J | 2006 | 应用化学2006，23，7： | 2 |
| 5 | Rituximab chimeric anti-CD20 monoclonal antibody treatment for adult refractory idiopathic thrombocytopenic purpura 显示文摘 | Braendstrup P Bjerrum OW Nielsen OJ | 2005 | Am J Hematol2005，78，4： | 1 |
| 6 | 7th Rankine Lecture: Engineering geology of Norwegian normally-consolidated marin clays as related to settlement of buildings显示文摘 | Bjerrum | 1967 | Geotechnique1967，17，2： | 1 |
| 7 | Engineering geology of Norwegian normally consolidated marine clays as related to the settlements of buildings显示文摘 | BJERRUM L | 1967 | Geotechnique1967，17，2： | 1 |
| 8 | Aluminum as anode for energy storage and conversion:a review显示文摘 | LI Q F BJERRUM N J | 2002 | Journal of Power Sources2002，110，： | 1 |
| 9 | An- tibiotic prescribing in general practice: striking differences between Italy (Ravenna)and Denmark(Funen)显示文摘 | Vaccheri A Bjerrum L Resi D Berman U Montanaro N | 2009 | J Antimicrob Chemother2009，50，6： | 1 |
| 10 | Oxygen reduction on carbon supported platinum catalysts in high temperature polymer electrolytes显示文摘 | LI Q F HJULER H A BJERRUM N J | 2000 | Electrochimica Acta2000，45，2526： | 1 |
| 11 | Progressive failure in slopes of overconsolidated plastic clay and clay shales显示文摘 | Bjerrum L | 1967 | ASCE Journal of the Soil Mechanics and Foundations Division1967，93，5： | 1 |
| 12 | Engineering geology of Norwegian normally consolidated marine clay as related to the settlements of buildings显示文摘 | BJERRUM L | 1967 | Geotechnique1967，17，2： | 1 |
| 13 | The influence of whole wheat feeding on Salmonella infection and gut flora compositiom in broiler显示文摘 | Bjerrum L Pedersen K Engberg R M | 2005 | Avian Disease2005，49，： | 1 |
| 14 | Aluminum as anode for energy storage and conversion: a review显示文摘 | LI Q BJERRUM N J | 2002 | J Power Sources2002，110，： | 1 |
| 15 | Engineering geology of Norwegiannormally consolidated marine clay as related to thesettlements of buildings显示文摘 | BJERRUM L | 1967 | Geotechnique1967，17，2： | 1 |
| 16 | Stability of strutted excavations in clay显示文摘 | BJERRUM L EIDE O | 1956 | Geotechnique1956，6，1： | 1 |
| 17 | The influence of whole wheat feeding on salmonella infection and gut flora composition in broilers显示文摘 | Bjerrum L Pedersen K Engberg R M | 2005 | Avian Dis2005，49，： | 1 |
| 18 | Nitrification performance and nitrifier community composition of a chemostat and a membrane-assisted bioreactor for the nitrification of sludge reject water 显示文摘 | Liebig T Wagner M Bjerrum L | 2001 | Bioproeess Biosyst Eng2001，24，4： | 1 |
| 19 | Stability of strutted excavations in clay显示文摘 | BJERRUM L EIDE O | 1956 | Geoteehaique1956，6，1： | 1 |
| 20 | Geotechnical problem involved in foundations of structures in the North Sea显示文摘 | Bjerrum J | 1973 | Geotechnique1973，23，3： | 1 |
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