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| 1 | Detection and Analysis of Routine Blood Indexes in Guike Pig显示文摘[Objectives] In this study,routine blood indexes of Guike pig were detected to clarify quantity variation and morphology distribution of different functional cells( red blood cells,white blood cells and platelets),thus providing reference data for immunological evaluation and disease resistance research of Guike pig. [Methods] Blood samples were collected from forty-four 49-day-old healthy Guike pigs( 22 males and 22 females) once a week for seven consecutive weeks to detect routine blood indexes,and the results were compared with routine blood indexes of Landrace pig and Enshi black pig. [Results]Among red blood cell indexes of Guike pig at different day ages,red blood cell count( RBC) and hemoglobin count( HGB) both reached the lowest levels( 6. 85 × 1012/L and 97. 1 g/L)at 70 days of age,which were significantly lower than the corresponding indexes at 49,56,63,84 and 91 days of age( P < 0. 05,similarly hereinafter); mean corpuscular hemoglobin( MCH) and mean corpuscular hemoglobin concentration( MCHC) demonstrated basically the same variation pattern,which both reached the lowest levels( 15. 11 pg and 275. 70 g/L) at 70 days of age and reached the highest levels( 16. 40 pg and 314. 30 g/L) at 91 days of age; hematocrit( HCT),mean corpuscular volume( MCV) and red blood cell distribution width( RDW) exhibited relatively stable changes with no significant differences among different day ages( P > 0. 05,similarly hereinafter). Among white blood cell indexes of Guike pig at different day ages,white blood cell count( WBC) reached the lowest level( 13. 71 × 109/L) at 63 days of age and reached the highest level( 17. 52 × 109/L) at 84 days of age,with significant differences; neutrophil count( NEUT)reached the lowest level( 3. 61 × 109/L) at 70 days of age,which was significantly lower than that at 49,56 and 77 days of age; lymphocyte count( LYMPH) exhibited relatively stable changes and was not significantly affected by the increase in day age. Among platelet indexes of Guike pig at different day ages,platelet count( PLT),thrombocytocrit( PCT) and platelet volume distribution width( PDW) exhibited relatively stable changes among different day ages,which were not significantly affected by the increase in day age. Compared to 35-day-old Landrace pig and 5-month-old Enshi black pig,most routine blood indexes of Guike pig were similar to or ranged between those of Landrace pig and Enshi black pig except HCT,mean platelet volume( MPV),PCT and PDW. [Conclusions]Guike pig has a certain uniqueness in routine blood indexes,which has developed a stable immune system at 49-91 days of age. | Qianlian SU Bin LI Jiaxing LIANG Yingning ZHOU Zhongwei CHEN Yibin QIN Bingxia LU Ying HE Qunpeng DUAN Dongfu JIANG Jingzhuan LU Wu ZHAO | 2018 | Agricultural Biotechnology2018，7，3： | 6 |
| 2 | Detection of Porcine Epidemic Diarrhea Virus in Guangxi Province from 2011 to 2014 and Sequence Analysis of Its M Gene显示文摘Detection of pigs epidemic diarrhea virus( PEDV) was conducted on 331 piglets diarrhea fecal samples collected in Nanning,Yulin and other 12 areas of Guangxi Province from January of 2011 to April of 2014 by the method of reverse transcription-polymerase chain reaction( RT-PCR). The results showed that the positive samples of PEDV were 210 and the positive rate was 63. 44%. The clone and sequencing of M gene was carried out on 25 positive samples. PEDV reference strains were selected from Gene Bank to conduct the sequence homology alignment analysis and the phylogenetic tree of M gene. The M gene homology and amino acid sequence identity between 25 isolated strains and 51 reference strains were 96. 0%- 99. 6% and 94. 3%- 99. 6%,respectively. The genetic variation analysis of M gene showed that the genetic relationship of PEDV prevalent strains in Guangxi Province from 2013 to 2014 was close to that of the prevalent strains in Beijing,Anhui,Wuhan,Hebei and Guangdong from 2010 to 2013,and which were far from that of the Chinese early isolates CH / S( Gen Bank number: JN547228),vaccine strain CV777( Gen Bank number: AF353511) and Attenuated DR13( Gen Bank number: JQ023162). Indicating that the PEDV strains prevalent in Guangxi in recent years showed significant variation with the early isolates. | Lu Bingxia Qin Yibin He Ying Li Yingying Liang Jiaxing Li Keyu Li Bin Su Qianlian Zhou Yingning Jiang Dongfu Lu Jingzhuan Bi Bingfen Liang Baozhong Duan Qunpeng Zhao Wu | 2016 | Animal Husbandry and Feed Science2016，8，1： | 3 |
| 3 | Isolation of Swine Corynebacteria and Analysis of 16S rDNA Gene显示文摘A strain of gram-positive bacillus was isolated from suppurative lung organs of nursery pigs in a pig farm,which was further characterized by morphological observation,cultivation test,biochemical test,drug sensitivity test,pathogenicity test and 16 S rDNA gene cloning and sequence analysis. The results showed that the isolate grew well in rabbit blood agar plate and horse serum tryptone soybean agar( TSA) plate under aerobic condition,which could lead to mortality of mice and were susceptible to cephalosporin antibiotics and fluoroquinolone antibiotics. Phylogenetic analysis showed that the isolate had close genetic evolutionary relationships with Corynebacterium bacteria,and the sequence of 16 S rRNA gene shared the homology of 91. 7%-98. 3% with the representative strain of corynebacteria,indicating the isolated strain was corynebacterium. | Qin Yibin He Pingping Lu Bingxia He Ying Li Bin Liang Jiaxing Duan Qunpeng Chen Zhongwei Bi Bingfen Zhou Yingning Su Qianlian Jiang Dongfu Li Keyu Lu Jingzhuan Zhao Wu | 2016 | Animal Husbandry and Feed Science2016，8，6： | 0 |
| 4 | Construction and Preliminary Application of RT-LAMP Detection Method for Swine Japanese B Encephalitis Virus显示文摘[Objective] This study aimed to establish a rapid,sensitive and specific method using reverse transcription loop-mediated isothermal amplification(RT-LAMP) technology to detect swine Japanese B encephalitis virus(JEV).[Method] Four specific LAMP primers were designed according to six loci the conservative region of JEV E gene sequence. Positive JEV RNA sample was used as a template for one-step amplification,and the reaction conditions and reaction system were optimized.[Result] Experimental results showed that the established method had high sensitivity,with the detection limit of 0. 5pg; specificity experiments indicated that the method had high specificity and there was no amplification reaction for other viral pathogens. The coincidence rate between detection results of RT-LAMP and RT-Nested-PCR was 90. 9%. After RT-LAMP reaction,a chemiluminescent agent was added for visual observation,which greatly reduced the detection time. This method required no special equipment but only a water bath,which was a simple,sensitive and rapid detection method for swine Japanese B encephalitis virus and could be applied in primary laboratories.[Conclusion] An RT-LAMP detection method for swine Japanese B encephalitis virus was successfully established and preliminarily applied in clinical practice. | Bingxia LU Lei LIU Yibin QIN Wu ZHAO Jiaxing LIANG Xuan GUO Mulan LI Bin LI Ying HE Qianlian SU Baozhong LIANG Bingfen BI Weijian HUANG | 2013 | Agricultural Biotechnology2013，2，4： | 0 |
| 5 | Phylogenetic Analysis of HN Gene of Eight Pigeon NDV Isolates in Guangxi显示文摘[Objective] The paper was to provide a basis for scientific prevention and control of pigeon Newcastle disease(ND).[Method] The HN gene of eight pigeon NDV strains isolated from different pigeon farms in Guangxi were amplified by RT-PCR,sequenced and analyzed.The molecular evolution characteristics of HN gene of pigeon NDV isolates in Guangxi was discussed.[Result] The nucleotide sequence length of HN gene of the eight NDV isolates was 1 716 bp,encoding 571 amino acids.They belonged to virulent group C,and the gene length characteristic of HN gene accorded with virulent strain.Analysis of nucleotide homologies indicated that the eight NDV isolates shared higher homology with genotype VIb,ranging from 90.4% to 99.5%.Phylogenetic tree analysis demonstrated that the genetic relationship between the eight NDV strains in Gangxi and the NDV isolates from Guangxi,Guangdong,Jilin,Liaoning,Yunnan and Heilongjiang during 2011 and 2013 was close.They were located in the same cladogram branch.[Conclusion] We assume that the eight pigeon NDV isolates in Guangxi all belong to the gene class II genotype VI b NDV. | Lu Bingxia Liang Jiaxing Duan Qunpeng Chen Zhongwei Jiang Dongfu Lu Jingzhuan Zhou Yingning Bi Bingfen He Ying Qin Yibin Li Bin Su Qianlian Zhao Wu | 2018 | Animal Husbandry and Feed Science2018，10，2： | 0 |
| 6 | Construction of Three Nucleic Acid Vaccines of Swine Hepatitis E Virus ORF2 Ger\e Continuous Fragment显示文摘In order to develop swine hepatitis E( HE) genetically engineering vaccines,specific primers of genes LB1,LB2,LB3 of swine hepatitis E virus were designed and used for amplification,DNA amplicons generated by PCR assays were directly cloned into T-A plasmid and expressed using pE ASY-M1 expression vector. Three recombinant eukaryotic expression plasmids of pE ASY-LB1,pE ASY-LB2 and pE ASY-LB3 were constructed. The eukaryotic expression plasmids of pE ASY-LB1,pE ASY-LB2,and pE ASY-LB3 were transfected into 293 T cells,and three target genes were detected by real-time fluorescent quantitative RT-PCR.The results confirmed that three eukaryotic expression plasmids were transfected into 293 Tcells and target protein was expressed. Analysis by SDS-PAGE electrophoresis and Western-blot indicated that three target proteins were expressed in 293 T cells transfected with eukaryotic expression plasmids of pE ASY-LB1,pE ASY-LB2 and pE ASY-LB3. Antigenicity studies indicated good HEV responses. Therefore,three recombinant DNAs of HEV ORF2 nucleic acid vaccine candidates were obtained,which might lay the foundation for further studies in the future. | Li Bin Su Qianlian Zhao Wu Qin Yibin Liang Jiaxing Xiao Aihuan Lu Bingxia Li Yingying He Ying Duan Qunpeng Jiang Jiajia Liang Baozhong | 2015 | Animal Husbandry and Feed Science2015，7，5： | 0 |
| 7 | Development and Application of an RT-PCR Method for Differentiation of PEDV Variant Strains and Classical Strains显示文摘In order to establish a rapid RT-PCR assay for detection of porcine epidemic diarrhea virus(PEDV),a pair of special primers was designed based on S gene sequence of PEDV published in Gen Bank.After optimization of the reaction system,a rapid RT-PCR method was established.Results showed that a fragment of 826 bp was successfully amplified only from the variant PEDV by RT-PCR,while the expected target fragment could not be amplified from TGEV,porcine rotavirus,porcine kobuvirus,PRRSV,PRV,CSFV,PPV.Sensitivity test of RT-PCR indicated that only 11.3 pg nuclear acids could be detected accurately and rapidly.A total of 123 samples collected from different farms in Guangxi Province were detected by the established RT-PCR,the positive rate of PEDV was 67.5%,and the positive rate of new PEDV was 86.7%(72/83).Therefore,the RT-PCR could be used as an effective tool for differentiating diagnosis of the highly pathogenic PEDV in epidemiological investigations. | Qin Yibin Lu Bingxia Zhao Wu He Ying Li Yingying Li Bin Liang Jiaxing Liang Baozhong Su Qianlian Jiang Dongfu Zhou Yingning | 2015 | Animal Husbandry and Feed Science2015，7，1： | 0 |
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