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| 1 | Antifibrogenic effects of vitamin D derivatives on mouse pancreatic stellate cells显示文摘AIM To study the molecular effects of three different D-vitamins, vitamin D2, vitamin D3 and calcipotriol, in pancreatic stellate cells(PSCs).METHODS Quiescent PSCs were isolated from mouse pancreas and activated in vitro by seeding on plastic surfaces. The cells were exposed to D-vitamins as primary cultures(early-activated PSCs) and upon re-culturing(fullyactivated cells). Exhibition of vitamin A-containing lipid droplets was visualized by oil-red staining. Expression of α-smooth muscle actin(α-SMA), a marker of PSC activation, was monitored by immunofluorescence and immunoblot analysis. The rate of DNA synthesis was quantified by 5-bromo-2'-deoxyuridine(Brd U) incorporation assays. Real-time PCR was employed to monitor gene expression, and protein levels of interleukin-6(IL-6) were measured by ELISA. Uptake of proline was determined using 18 F-proline.RESULTS Sustained culture of originally quiescent PSCs inducedcell proliferation, loss of lipid droplets and exhibition of stress fibers, indicating cell activation. When added to PSCs in primary culture, all three D-vitamins diminished expression of α-SMA(to 32%-39% of the level of control cells; P < 0.05) and increased the storage of lipids(scores from 1.97-2.15 on a scale from 0-3; controls: 1.49; P < 0.05). No such effects were observed when D-vitamins were added to fully-activated cells, while incorporation of Brd U remained unaffected under both experimental conditions. Treatment of re-cultured PSCs with D-vitamins was associated with lower expression of IL-6(-42% to-49%; P < 0.05; also confirmed at the protein level) and increased expression of the vitamin D receptor gene(209%-321% vs controls; P < 0.05). There was no effect of D-vitamins on the expression of transforming growth factor-β1 and collagen type 1(chain α1). The lowest uptake of proline, a main component of collagen, was observed in calcipotriol-treated PSCs.CONCLUSION The three D-vitamins inhibit, with similar efficiencies, activation of PSCs in vitro, but cannot reverse the phenotype once the cells are fully activated. | Peter Wallbaum Sarah Rohde Luise Ehlers Falko Lange Alexander Hohn Carina Bergner Sarah Marie Schwarzenbock Bernd Joachim Krause Robert Jaster | 2018 | World Journal of Gastroenterology2018，24，2： | 3 |
| 2 | Levels and effects ofdifferent forms of anxiety during pregnancy after a priormiscarriage显示文摘 | FERTL K I BERGNER A BEYER R | 2009 | Eur J Obstet Gynecol Reprod Biol2009，142，1： | 1 |
| 3 | Improved total varia- tion-based CT image reconstruction applied to clinical data显示文摘 | Ludwig Ritschl Frank Bergner | 2011 | Physics in Medicine and Biology2011，56，6： | 1 |
| 4 | Cytokeratin 5/6 immunohistochemistry assists the differential diagnosis of atypical prolixferations of the breast显示文摘 | Otterbach F Bankfalvi A Bergner S | 2000 | Histopathology2000，37，： | 1 |
| 5 | Glucosinolatehydrolysis in Lepidium sativum:identification of the thiocyanate-form-ing protein显示文摘 | BUROW M BERGNER A GERSHENZON J | 2006 | Plant Mol Biol2006，63，： | 1 |
| 6 | Neurologic compiications of Churg Strauss syndrome a prospective monocentric study显示文摘 | Wolf J Bergner R Mutallib S | 2010 | Eur-J-Neurol2010，17，4： | 1 |
| 7 | Role of chemotherapy additional to high-dose methotrexate for primary central nervous system lymphoma(PCNSL)显示文摘 | Bergner N Monsef I Illerhaus G | 2012 | Cochrane Database Syst Rev2012，11，00： | 1 |
| 8 | Levels and effects of different forms of anxiety during pregnancy after a prior miscarriage显示文摘 | Fertl KI Bergner A Beyer R | 2009 | Eur J Obstet Gynecol Reprod Biol2009，142，1： | 1 |
| 9 | Profile:validation of a health status measure显示文摘 | Bergner M Bobbitt RA Pollard W E The Sickness impact | 1976 | Med care1976，14，1： | 1 |
| 10 | Colonizing while migrating: how do individual enteric neural crest cells behave?显示文摘 | Young HM Bergner AJ Simpson MJ | 2014 | BMC BioI2014，12，： | 1 |
| 11 | Preservation of residual renal function in dialysis patients: effects of dialysis-technique-related-factors 显示文摘 | Lang SM Bergner A Topfer M | 2001 | Perit Dia Int2001，21，1： | 1 |
| 12 | Pregnancy after early pregnancy loss:a prospective study of anxiety, depressive symptomatology and coping 显示文摘 | Bergner A Beyer R Klapp BF | 2007 | J Psychosom Obstet Gynaecol2007，28，1： | 1 |
| 13 | Third order nonlinear optical properties of substituted poly (p-phenylene vinylene) derivatives 显示文摘 | H L Li S Rentsch H Bergner | 1991 | Institute of Physics Conference Series1991，126，： | 1 |
| 14 | Spatio chromatic decorrelation for color image compression显示文摘 | DREW M S BERGNER S | 2008 | Signal Processing Image Communication2008，23，8： | 1 |
| 15 | Prediction of short-term survival in patients with advanced nonsmall cell lung cancer following chemotherapy based on 2-deoxy-2- fluoro-Dglucosepositron emission tomography:a feasibility study显示文摘 | Dimitrakopoulou-Strauss A Hoffmann M Bergner R | 2007 | Mol Imaging Biol2007，9，5： | 1 |
| 16 | Levels and effects of different forms of anxiety during pregnancy after a prior miscarriage显示文摘 | Fertl KI Bergner A Beyer R | 2009 | Eur J Obstet Gynecol Reprod Biol2009，142，1： | 1 |
| 17 | Mamary gland morpholo- gy in Sprague-Dawley rats following treatment with an organochlo-rine mixture in utero and neonatal genistein 显示文摘 | Godschalk MF Unice KA Bergner D | 2004 | Toxicol Sci2004，77，1： | 1 |
| 18 | The sickness impact profile : validation of a health status measure 显示文摘 | Bergner M Bobbit RA Pollard WE | 1976 | Med Care1976，14，1： | 1 |
| 19 | High bone-binding capacity of ibandronate in hemodialysis patients 显示文摘 | Bergner R Henrich D Hoffmann M | 2005 | Int J Clin Pharmacol Res2005，25，3： | 1 |
| 20 | The Sickness impact Profile : validation of a health status measure 显示文摘 | Bergner M Bobbitt RA Pollard WE | 1976 | Med Care1976，14，1： | 1 |
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