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| 1 | Orthotopic transplantation model of human gastrointestinal cancer and detection of micrometastases显示文摘AIM To establish a relevant animal model ofhuman gastrointestinal cancer, which can beused for repetitive investigations, so as toimprove our understanding and management ofcarcinogenesis and cancer metastasis.METHODS Intact tissues of human colorectaland pancreatic cancers were transplanted innude mice. The biological characteristics of theoriginal and the corresponding transplantedtumors were investigated by HE staining, PASstaining and immunostaining. The metastases inthe livers and lungs of nude mice wereinvestigated by immunostaining withbiotinylated mab KL-1 and by RT-PCR using CK20specific primers.RESULTS There were totally 9 of 16 surgicalspecimens growing in nude mice subcutaneouslyand/.or orthotopically (4 of 6 colorectal and 5 of10 pancreatic cancer). Tumor cell content of thespecimens and freezing of tissue specimens areimportant factors influencing the growth oftransplanted tumor. In the group of fresh tumortissues with greater than 50% tumor cellcontent, the success rate of the transplantationwas 100% (3 cases of pancreatic cancer and 3cases of colorectal cancer). The orthotopicallytransplanted tumors resemble the original tumormorphologically and biologically, including TAAexpression such as CEA byimmunohistochemistry, and CEA level in theserum of mice. Ki-67 labeling index and theexpression of TAA especially K-ras, 17-lA andRA-96, are associated with the potential of tumorgrowth in nude mice. Micrometastases in thelungs and livers of tumor bearing mice can bedetected by immunostaining with biotinylatedmab KL-1 and CK20-specific RT-PCR.CONCLUSION An orthotopic transplantationmodel for human colon and pancreatic cancer innude mice has been set up. We have alsoestablished sensitive detection methods withCK-immunohistochemistry and CK20-RT-PCR tostudy xenotransplanted human cancer and itsmetastatic cancer cells in the liver and lung ofnude mice. This study may be helpful inunderstanding the mechanism of cancermetastasis and in developing new diagnosticmethods and therapeutic strategies formetastases including micrometastases. | Jun Hui Cui~1 Uwe Krueger~2 Doris Henne-Bruns~2 Bemd Kremer~2 Holger Kalthoff~2 ~1Department of General Surgery,First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,Zhejiang Province,China ~2Department of General Surgery,Christian-Albrechts-University,Kiel,GermanyDr.Jun Hui Cui graduated from Zhejiang Medical University in 1984,earned master degree in 1990,studied in the Surgical Department of Kiel University and worked in the Lab of Molecular Oncology of Kiel University from 1994-1997achieved M.D.from Kiel University.Germany,now associate professor of surgery,specialized in colorectal oncology.Adviser of graduated student for master degree,having 20 publications published in key Chinese or English journals. | 2001 | World Journal of Gastroenterology2001，7，3： | 19 |
| 2 | New Zircon U-Pb Geochronology of the Post-kinematic Granitic Plutons in the Diancang Shan Metamorphic Massif along the Ailao Shan-Red River Shear Zone and Its Geological Implications显示文摘Ailao 掸人红河差错地区是在到东北和印度支那块的 Yangtze 块之间的边界到西南。它由于它在印度支那块的向东南的挤出的角色是一个重要构造地区在期间并且对印第安人欧亚的碰撞随后。Diancang 掸人(DCS ) 高级变形建筑群,沿着 Ailao 掸人红河(ASRR ) 在西北延期定位了砍地区,是 ASRR 构造的带的代表性的变形建筑群。结构并且在高级变形岩石中的砍的岩石的微结构分析表明他们是协调的与固态高温度的可锻的变丑,它被归因于沿着 ASRR 的左侧面的砍砍地区。新 LA-ICP-MS 锆石 U-Pb geochronological 和微结构 kinematic 以后学习花岗石的 plutons 在结束上提供直接时间限制在 ASRR 的可锻的左侧面的砍和变形山岳的发掘砍地区。这被建议沿着 ASRR 的左侧面的砍砍在 ca 结束的地区。以相对降低温度或减少的温度条件的 21 妈。在期间或在在 ca 的年轻的堤的炮兵阵地以后。21 妈,快速的易碎的变丑事件发生了,它让 DCS 山岳开始快 uplift/exhumation 并且冷却到浅外壳的水平。 | CAO Shuyun LIU Junlai Bemd LEISS ZHAO Chunqiang | 2010 | Acta Geologica Sinica(English Edition)2010，84，6： | 9 |
| 3 | Single Cr atom catalytic growth of graphene显示文摘 | Huy Q. Ta Uang Zhao Wanjian Yin Darius Pohl Bemd Rellinghaus Thomas Gemming Barbara Trzebicka Justinas Palisaitis Gao Jing Per O. A. Persson Zhongfan Liu Alicja Bachmatiuk Mark H. Rummeli | 2018 | Nano Research2018，11，5： | 3 |
| 4 | Microstruring of materials by pulsed laser focusing and projection technique显示文摘 | Horst Exner Bemd Keiper Peter Meja | 1999 | SPIE1999，3618，： | 2 |
| 5 | The influence of EDDS on the uptake of heavy metal in hydroponically grown sunflowers显示文摘 | Susan T Rainer S Bemd N | 2006 | Chemosphere2006，62，： | 1 |
| 6 | Total degradation of EDTA by mixed cultures and abacterial isolate显示文摘 | | 1992 | Applied and Environmental Microbiology1992，58，2： | 1 |
| 7 | The elastic modulus of misaligned short fiber reinforced polymers显示文摘 | Shao-Yun Fu & Bemd Lauke | 1998 | Comp Sci Technol1998，58，： | 1 |
| 8 | Bacterial load in relation to vacuum-assisted closure wound therapy: a prospective randomized trial 显示文摘 | Moues CM Vos MC van den Bemd GJ | 2004 | Wound Repair Regen2004，12，1： | 1 |
| 9 | Comparing convention- al gauze therapy to vacuum - assisted closure wound therapy显示文摘 | Moues CM van den Bemd GJ Heule F et 81 | 2007 | J Plast Recons: Aesthet Stag2007，60，： | 1 |
| 10 | Comparing conventional gauze therapy to vacuum-assisted closure wound therapy:aprospective randomized trial显示文摘 | Mous CM van den Bemd GJ Heule F | 2007 | J Plast Reconstr Aesthet Surg2007，60，6： | 1 |
| 11 | Rheological characterization of water-based slurries for the tape casting process 显示文摘 | BITTERLICH Bemd LUTZ Hristiane ROOSEN Reas | 2002 | Ceram hat2002，28，： | 1 |
| 12 | Polyelectrolyte multilayer membranes for pervaporation separation of alcohol/water mixtures显示文摘 | Toutianoush Ali Krasemann Lutz Bemd Tieke | 2002 | Colloids and Surfaces A:Physicochemical and Engineering Aspects2002，，： | 1 |
| 13 | Bacterial load in relation to vacuum-assisted closure wound therapy:a prospective randomized trial显示文摘 | Moues CM Vos MC van den Bemd GJ | | 0，，： | 1 |
| 14 | Syntheses and Properties of Two Heterocyclically Substituted Hypericin Derivatives: 10, 11- Dibenzothiazolyl-10, 11-didemethylhypericin and 10, 11-Dibenzoxa- zolyl- 10, 11-didemethylhyperi-cin显示文摘 | Bemd L Yulita P Christoph E | 2005 | Monatshefte Chemie2005，136，5： | 1 |
| 15 | Effect of the Interface Morphology on the Fill Factor of Plastic Solar Cells 显示文摘 | V Djara J C Bemde | 2005 | Thin Solid Films2005，493，12： | 1 |
| 16 | Quantization effects on digital watermarks显示文摘 | Joachim J Eggers Bemd Girod | 2001 | Signal Processing2001，81，： | 1 |
| 17 | Utilization of sewage sludge ashes in the brick and tile Industry显示文摘 | Bemd Weibusch | 1997 | Wat Sci Tech1997，36，11： | 1 |
| 18 | An experimental bribery game 显示文摘 | klaus abbin k bemd irl enbusch el ke renner | 2002 | Journal of law economics & organization2002，，18： | 1 |
| 19 | Generational Accounting in Europe显示文摘 | Bemd Raffelhuschern | 1999 | American Economic Review1999，89，2： | 1 |
| 20 | Mutation rate in human microsatel- lites : influence of the structure and length of the tandem repeats显示文摘 | Bemd B Michael K Franz | 1998 | Am J Hum Genet1998，62，： | 1 |
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