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| 1 | Using the polymerase chain reaction coupled with denaturing gradient gel electrophoresis to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis显示文摘AIM: To investigate the use of PCR and DGGE to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted severe AP.METHODS: Patients with biochemical and clinical evidence of acute pancreatitis and an APACHE Ⅱ score ≥8 were enrolled. PCR and DGGE were employed to detect bacterial translocation in blood samples collected on d1,3, and 8 after the admission. Standard microbial blood cultures were taken when there was clinical evidence of sepsis or when felt to be clinically indicated by the supervising team.RESULTS: Six patients were included. Of all the patients investigated, only one developed septic complications;the others had uneventful illness. Bacteria were detected using PCR in 4 of the 17 collected blood samples. The patient with sepsis was PCR-positive in two samples (taken on d 1 and 3), despite three negative blood cultures. Using DGGE and specific primers, the bacteria in all blood specimens which tested positive for the presence of bacterial DNA were identified as E coli.CONCLUSION: Our study confirmed thatunlike traditional microbiological techniques, PCR can detect the presence of bacteria in the blood of patients with severe AP. Therefore, this latter method in conjunction with DGGE is potentially an extremely useful tool in predicting septic morbidity and evaluating patients with the disease. Further research using increased numbers of patients, in particular those patients with necrosis and sepsis, is required to assess the reliability of PCR and DGGE in the rapid diagnosis of infection in AP. | Callum B Pearce Vitaly Zinkevich Iwona Beech Viera Funjika Ana Garcia Ruiz Afraa Aladawi Hamish D Duncan | 2005 | World Journal of Gastroenterology2005，11，45： | 11 |
| 2 | Resolution of stroke deficit following contralateral grafts of conditionally immortal neuroepithelial stem cells显示文摘 | Veizovic T Beech JS Stroemer RP | 2001 | Stroke2001，32，4： | 2 |
| 3 | Biocorrosion: towards understanding interactions between biofilms and metals显示文摘 | Iwona B Beech Jan Sunner | 2004 | Current Opinion in Biotechnology2004，，3： | 2 |
| 4 | Molecular and morphological characterization of the tapeworm Taenia hydatigena (Pallas, 1766) in sheep from Iran显示文摘 | S. Rostami R. Salavati R.N. Beech Z. Babaei M. Sharbatkhori M.R. Baneshi E. Hajialilo H. Shad M.F. Harandi | 2013 | Journal of Helminthology2013，，2： | 2 |
| 5 | Stabilityof geosynthetic-soil layered systems on slope 显示文摘 | Giroud J P Williams N D Peite T Beech J F | 1995 | Gepsynthetics International1995，2，6： | 2 |
| 6 | Atomic force microscopy study of microbiological influenced corrosion SRB biofilms on steel surface显示文摘 | Steele A Goddard D T Beech I B | 1994 | International Biodeterioration and Biodegradation1994，34，： | 1 |
| 7 | Origin of gas bubbles in aluminum显示文摘 | Tiwari S N Beech J | 1978 | Metal Sci1978，12，： | 1 |
| 8 | Stem cell grafts resolve sensory dysfunction following MCAO in the rat显示文摘 | Veizovic T Stroemer P Beech J | 1999 | J Cereb Blood Flou Metab1999，19，1： | 1 |
| 9 | The New Radicals显示文摘 | Beech H | 2004 | Time(Asian edition)2004，，2： | 1 |
| 10 | Maternal and neonataltetanus显示文摘 | ThwaitesC L Beeching N J Newton C R | 2015 | Lancet2015，385，9965： | 1 |
| 11 | Dental management of patients irradiated for head and neck cancer显示文摘 | BEECH N ROBINSON S PORCEDDU S | 2014 | Australian dental journal2014，59，1： | 1 |
| 12 | Evoluation of cassava (Manihot esculenta Crantz) germplasm collection using RAPD markers显示文摘 | MARMEY Philippe JOHN R Beeching | 1994 | Euphytica1994，74，： | 1 |
| 13 | Biocorrosion : towards understand- ing interactions between biofilms and metals 显示文摘 | Beech I B Sunner J | 2004 | Curr Opin Biotechnol2004，15，3： | 1 |
| 14 | Workplace violence in the health care: A review of staff training and integration of training evaluation models 显示文摘 | Beech B Leather P | 2006 | Aggression and Violent Behavior2006，11，： | 1 |
| 15 | Suppression of breast cancer metastasis through the inhibition of VEGF-rnediated tumor angiogenesis显示文摘 | ZhangJ Lu A Beech D | 2007 | Cancer Ther2007，5，： | 1 |
| 16 | Design of soil layer-geosynthetic systems overlying voids显示文摘 | Giroud J P Bonaparte R Beech J F | 1990 | Geotextiles and Geomembranes1990，9，： | 1 |
| 17 | Pyogenic lung infections : factors for predicting clinical outcome of lung abscess and thoracic empyema显示文摘 | Mwandumba HC Beeching NJ | 2000 | Curt Opin Pulm Med2000，6，3： | 1 |
| 18 | Functions of large conductanc Ca^2+ - activated ( KCa ), delayed rectifier ( Kv ) and background K^+ channels in the control of membrane potential in rabbit renal arcuate artery 显示文摘 | Prior HM Yates MS Beech DJ | 1998 | J Physiol1998，511，1： | 1 |
| 19 | Mutations in ATP2C1, encoding a calcium pump, cause Halley-Halley disease 显示文摘 | Hu Z Bonifas JM Beech J | 2000 | Nat Genet Genet2000，24，1： | 1 |
| 20 | Effect of cooling rate on the morphology of sulphide inclusions显示文摘 | Mohla P beech J | | J Iron Steel Inst0，207，： | 1 |
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