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| 1 | Using the polymerase chain reaction coupled with denaturing gradient gel electrophoresis to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis显示文摘AIM: To investigate the use of PCR and DGGE to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted severe AP.METHODS: Patients with biochemical and clinical evidence of acute pancreatitis and an APACHE Ⅱ score ≥8 were enrolled. PCR and DGGE were employed to detect bacterial translocation in blood samples collected on d1,3, and 8 after the admission. Standard microbial blood cultures were taken when there was clinical evidence of sepsis or when felt to be clinically indicated by the supervising team.RESULTS: Six patients were included. Of all the patients investigated, only one developed septic complications;the others had uneventful illness. Bacteria were detected using PCR in 4 of the 17 collected blood samples. The patient with sepsis was PCR-positive in two samples (taken on d 1 and 3), despite three negative blood cultures. Using DGGE and specific primers, the bacteria in all blood specimens which tested positive for the presence of bacterial DNA were identified as E coli.CONCLUSION: Our study confirmed thatunlike traditional microbiological techniques, PCR can detect the presence of bacteria in the blood of patients with severe AP. Therefore, this latter method in conjunction with DGGE is potentially an extremely useful tool in predicting septic morbidity and evaluating patients with the disease. Further research using increased numbers of patients, in particular those patients with necrosis and sepsis, is required to assess the reliability of PCR and DGGE in the rapid diagnosis of infection in AP. | Callum B Pearce Vitaly Zinkevich Iwona Beech Viera Funjika Ana Garcia Ruiz Afraa Aladawi Hamish D Duncan | 2005 | World Journal of Gastroenterology2005，11，45： | 11 |
| 2 | Stabilityof geosynthetic-soil layered systems on slope 显示文摘 | Giroud J P Williams N D Peite T Beech J F | 1995 | Gepsynthetics International1995，2，6： | 2 |
| 3 | Atomic force microscopy study of microbiological influenced corrosion SRB biofilms on steel surface显示文摘 | Steele A Goddard D T Beech I B | 1994 | International Biodeterioration and Biodegradation1994，34，： | 1 |
| 4 | Suppression of breast cancer metastasis through the inhibition of VEGF-rnediated tumor angiogenesis显示文摘 | ZhangJ Lu A Beech D | 2007 | Cancer Ther2007，5，： | 1 |
| 5 | Neuroprotective effect of aminoguanidine on transient focal ischemia in the rat brain显示文摘 | Beech JS Rayne RC | 2001 | Brain Res2001，905，12： | 1 |
| 6 | Neuroprotective effect of aminoguanidine on transient focal ischaemia in the rat brain显示文摘 | Cash D Beech JS Rayne RC | 2001 | Brain Res2001，905，： | 1 |
| 7 | Expression of PH-20 in normal and neoplastic breast tissue显示文摘 | Beech D J Madan A K Deng N | 2002 | J Surg Res2002，103，： | 1 |
| 8 | Soft tissue sarcoma clinical practice guidelines in oncology显示文摘 | Demetri GD Baker LH Beech D | 2005 | J Natl Compr Canc Netw2005，3，2： | 1 |
| 9 | Neuroprotective effect of aminoguanidine on transient focal ischaemia in the rat brain显示文摘 | Cash D Beech J S Rayne R C | 2001 | Brain Ret2001，29，： | 1 |
| 10 | Compara- tive studies of bacterial biofilms on steel surfaces using atomic force microscopy and environmental scanning electron microsco- py显示文摘 | BEECH I A CHEUNG C W S JOHNSON D B | 1996 | Biofouling1996，10，13： | 1 |
| 11 | Suppression of breast cancer metastasis through the inhibition of VEGF-mediated tumor an-giogenesis显示文摘 | Zhang J Lu A Beech D | | 0，，02： | 1 |
| 12 | Factors affecting heat generation in tire Mc- Spadden compaction technique显示文摘 | Figdor D Beech DR Waterson J | 1983 | J Dent Res1983，13，2： | 1 |
| 13 | Synchronous occurrence of glioblastoma multiformity and esophagealadeno-carcinoma显示文摘 | Beech D J Madan A K Aliabadi Wahle S S | 2003 | Am Surg2003，69，2： | 1 |
| 14 | The consititution of bone china显示文摘 | Beech D G | 1959 | The A T Green Book1959，13，5： | 1 |
| 15 | Molecular cloning and characterization of novel glutamate-gated chloride channel subunits from Schistosoma mansoni显示文摘 | Vanessa D Beech RN Claudia W et d | 2013 | PloS Pathogens2013，9，10： | 1 |
| 16 | Comparative studies of bacterial biofilms on steel surfaces using atomic force microscopy and environmental scanning electron microscopy 显示文摘 | Beech I B Cheung C W S Johnson D B | 1996 | Biofouling1996，10，： | 1 |
| 17 | Comparative studies of bacterial biofilms on surfaces using atomic force microscopy and environmental scanning electron microscopy显示文摘 | Beech J B Cheung C W S Johnson D B | 1996 | Biofouling1996，10，2： | 1 |
| 18 | TRPCI : store-operated cationic channel subunit 显示文摘 | Beech DJ Xu SZ McHugh D | 2003 | Cell Calcum2003，33，56： | 1 |
| 19 | Anthelmintic re- sistance : markers for resistance, or susceptibility? 显示文摘 | BEECH R N SKUCE P BARTLEY D J | 2011 | Parasitolo- gy2011，138，2： | 1 |
| 20 | Factors affecting survival and intergration following microinjection of novel DNA into rainbow trout eggs显示文摘 | Penman D J Beeching A J S Maclean N | 1990 | Aquaculture1990，85，： | 1 |
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