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22篇 您的检索式：作者名="Becker DI"

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	题名	作者	年代	出处	被引量
1	Diversity shifts of rumen bacteria induced by dietary forages in dairy cows and quantification of the changed bacteria using a new primer design strategy显示文摘The partial 16 S r RNA gene sequences(100 to 500 bp) were widely used to reveal rumen bacterial composition influenced by diets, while quantification of the changed uncultured bacteria was inconvenient due to difficult designing of specific primers based on short sequences. This study evaluated the effect of forage resources on rumen bacterial diversity and developed new strategy for primer design based on short sequences to quantify the changed uncultured bacteria. Denaturing gradient gel electrophoresis(DGGE) analysis and subsequent band sequencing were used to reveal the distinct rumen bacteria composition in cows fed with two forage sources(single corn stover vs. mixed forages including alfalfa hay and corn silage). The bacterial diversity in the rumen of dairy cows fed with corn stover was lower than that with mixed forages(P<0.05). The bacterium named R-UB affiliating to uncultured Succinivibrionaceae was identified, and it was abundant in the rumen of cows fed with mixed forages compared to corn stover. The full length 16 S r RNA gene sequences with identity of >97% to the R-UB 16 S r RNA gene sequence were obtained from Gen Bank and used to design specific primers to quantify uncultured bacterium R-UB. All sequences of amplicon from the new primers were of 100% identity to R-UB sequences indicating the high specificity of new primers. Quantitative PCR confirmed that abundance of R-UB in the rumen of cows fed with corn stover was lower than those fed with mixed forages(P<0.01). New strategy for designing primers based on partial 16 S r RNA genes to quantify targeted uncultured bacteria was successfully developed. The rumen bacteria descending significantly in the cows fed corn stover compared to those fed mixed forages was identified as uncultured R-UB from Succinivibrionaceae.	JIN Di ZHAO Sheng-guo ZHANG Yang-dong SUN Peng BU Deng-pan Yves Beckers WANG Jia-qi	2016	Journal of Integrative Agriculture2016，15，11：	2
2	A short history of MADS-box genes in plants显示文摘	THEISSEN G BECKER A DI ROSA A	2000	Plant Molecular Biology2000，42，1：	1
3	Renal malignant fibrous histiocytoma显示文摘	JosephTJ Becker DI Turton AF	1991	Urology1991，37，：	1
4	Research evaluation and prospective diagnosis of dementia with Lewy bodies显示文摘	Lopez OL Becker JT Kaufer DI	2002	Arch Neurol2002，59，：	1
5	A short history of MADS-box genes in plants显示文摘	Theissen G Becker A Rosa Di A	2000	Plant Molecular Biology2000，42，1：	1
6	Research evaluation and prospective diagnosis of dementia with Lewy bodies显示文摘	Lopez OL Becker JT Kaufer DI	2002	Arch Neurol2002，59，：	1
7	Functional studies of human skin disease and deafness-associated connexin 30 mutations显示文摘	Common JE Becker D Di WL	2002	Biochemical and Biophysical Research Communications2002，298，5：	1
8	A short history ofMADS-box genes in plants 显示文摘	Theissen G Becker A Di Rosa A	2000	Plant Molecular Biology2000，42，1：	1
9	A short history of MADS-box genes in plants显示文摘	Günter Theissen Annette Becker Alexandra Di Rosa Akira Kanno Jan T. Kim Thomas Münster Kai-Uwe Winter Heinz Saedler	2000	Plant Molecular Biology2000，，1：	1
10	A short history of MADS-box genes in plants显示文摘	Theissen G Becker A di Rosa A	2000	Plant Mol Biol2000，42，：	1
11	A suggested cause of the fire-induced collapse of the world trade towers显示文摘	Quintiere J G Di Marzom M Becker R	2002	Fire Safety Journal2002，37，：	1
12	A short history of MADS- box genes in plants 显示文摘	Theissen G Becker A Di RA	2000	Plant Mol Biol2000，42，：	1
13	Renal malignant fibrous histiocytoma 显示文摘	Joseph TJ Becker DI Turton AF	1991	Urology1991，37，：	1
14	A short history of MADS-box genes in plants显示文摘	Günter Theissen Annette Becker Alexandra Di Rosa Akira Kanno Jan T. Kim Thomas Münster Kai-Uwe Winter Heinz Saedler	2000	Plant Molecular Biology2000，，1：	1
15	A short history of MADS-box genes in plants显示文摘	Theissen G Becker A Di Rosa A	2000	Plant Mol Biol2000，42，1：	1
16	A short history of MADS-box genes in plants显示文摘	THEISSEN G Becker A Di Rosa A Kanno A Kim J Thomas Munster Winter K Saedler H	2000	Plant Mol Biol2000，42，：	1
17	A short history of MADS -box genes in plants显示文摘	THEISSEN G BECKER A DI ROSA A	2000	Plant Molecular Biology2000，42，：	1
18	A short history of MADS -box genes in plants显示文摘	TI-IEII3EN G BECKER A DI ROSA A	2000	Plant Molecular Biology2000，42，1：	1
19	A short history of MADS-box genes in plants显示文摘	Theissen G Becker A Di Rosa A	2000	Plant Molecular Biology2000，42，1：	1
20	A short history of MADS-box genes in plants显示文摘	Günter Theissen Annette Becker Alexandra Di Rosa Akira Kanno Jan T. Kim Thomas Münster Kai-Uwe Winter Heinz Saedler	2000	Plant Molecular Biology2000，，1：	1

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